scholarly journals The Use of Hierarchical Sampling in the Surveillance Program for Plum pox virus Incidence in the United States

Plant Disease ◽  
2002 ◽  
Vol 86 (3) ◽  
pp. 259-263 ◽  
Author(s):  
G. Hughes ◽  
T. R. Gottwald ◽  
L. Levy

Plum pox virus (PPV) was first reported in North America in late 1999. As part of the eradication effort mounted in response to this finding, a Plum Pox Virus National Surveillance Program has been implemented in the United States. The survey method adopted for this program is a form of “group testing” that utilizes relationships between disease incidence at two levels in a spatial hierarchy, known as hierarchical sampling. Leaf material is collected from groups of four trees and tested by enzyme-linked immunosorbent assay (ELISA) for PPV infection without distinguishing between the individual trees in a group. PPV incidence at the scale of the individual tree is then calculated by means of a formula. Incidence data were collected during an initial survey conducted by the Pennsylvania Department of Agriculture from nine PPV-infected orchards in the fall of 1999 and from an additional 11 PPV-infected orchards during the spring and summer of 2000. The pattern of PPV-infected trees at the within-group scale was weakly aggregated. As a result, an effective sample size of v = 3.2, rather than the nominal group size of four, should be used in the formula to calculate an estimate of incidence at the scale of the individual tree from observations of incidence collected at the group scale.

Plant Disease ◽  
2014 ◽  
Vol 98 (7) ◽  
pp. 916-923 ◽  
Author(s):  
C. H. Bock ◽  
B. W. Wood ◽  
K. L. Stevenson ◽  
R. S. Arias

Fusicladium effusum causes pecan scab, which is the most destructive disease of pecan orchards in the United States. Conidia of the pathogen are spread by rain splash and wind. The fungus is pathogenically diverse; yet there is no information on its genetic diversity or population genetics. Universally primed polymerase chain reaction (UP-PCR) was used to investigate the genetic diversity and population structure on a hierarchical sample of 194 isolates collected from 11 orchard locations from Florida to Texas, consisting of three to four isolates from each of five to six trees at each location. Genetic variation was high throughout the region, with all but nine of the multilocus haplotypes being unique. Nei's average gene diversity ranged from 0.083 for a population from Mississippi to 0.160 for a population from Kansas. An analysis of molecular variance of the hierarchically sampled populations found that the majority of the genetic variability (82.6%) occurred at the scale of the individual tree and only relatively small amounts among populations in trees from an orchard (5.0%) or within groups (i.e., orchard location populations) (12.5%). The results suggest little population differentiation in F. effusum in the southeastern United States, although φpt values of genetic distance for pairwise comparisons indicated some populations could be differentiated from others. There was evidence of linkage disequilibrium in certain populations, and the common occurrence of asexual reproduction in F. effusum could lead to measurable linkage disequilibrium under certain circumstances. However, the degree of genetic diversity and the scale over which diversity is distributed is evidence that F. effusum undergoes regular recombination despite no known sexual stage.


2015 ◽  
Vol 36-37 (1) ◽  
pp. 163-183
Author(s):  
Paul Taylor

John Rae, a Scottish antiquarian collector and spirit merchant, played a highly prominent role in the local natural history societies and exhibitions of nineteenth-century Aberdeen. While he modestly described his collection of archaeological lithics and other artefacts, principally drawn from Aberdeenshire but including some items from as far afield as the United States, as a mere ‘routh o’ auld nick-nackets' (abundance of old knick-knacks), a contemporary singled it out as ‘the best known in private hands' (Daily Free Press 4/5/91). After Rae's death, Glasgow Museums, National Museums Scotland, the University of Aberdeen Museum and the Pitt Rivers Museum in Oxford, as well as numerous individual private collectors, purchased items from the collection. Making use of historical and archive materials to explore the individual biography of Rae and his collection, this article examines how Rae's collecting and other antiquarian activities represent and mirror wider developments in both the ‘amateur’ antiquarianism carried out by Rae and his fellow collectors for reasons of self-improvement and moral education, and the ‘professional’ antiquarianism of the museums which purchased his artefacts. Considered in its wider nineteenth-century context, this is a representative case study of the early development of archaeology in the wider intellectual, scientific and social context of the era.


1980 ◽  
Vol 1 (8) ◽  
pp. 3-6
Author(s):  
George J. Annas

In an extraordinary and highly controversial 5-4 decision, the United States Supreme Court decided on June 30, 1980, that the United States Constitution does not require either the federal government or the individual states to fund medically necessary abortions for poor women who qualify for Medicaid.At issue in this case is the constitutionality of the Hyde Amendment. The applicable 1980 version provides:|N]one of the funds provided by this joint resolution shall be used to perform abortions except where the life of the mother would be endangered if the fetus were carried to term; or except for such medical procedures necessary for the victims of rape or incest when such rape or incest has been reported promptly to a law enforcement agency or public health service, (emphasis supplied)


2020 ◽  
pp. 000313482096005
Author(s):  
Michael Sarap ◽  
Julie Conyers ◽  
Crystal Cunningham ◽  
Adam Deutchman ◽  
Glenn Levine ◽  
...  

Rural surgeons from disparate areas of the United States report on the effects of the COVID-19 pandemic in their communities as the virus has spread across the country. The pandemic has brought significant changes to the professional, economic, and social lives of the individual surgeons and their communities.


2020 ◽  
Vol 96 (2) ◽  
pp. 419-437
Author(s):  
Xiangfeng Yang

Abstract Ample evidence exists that China was caught off guard by the Trump administration's onslaught of punishing acts—the trade war being a prime, but far from the only, example. This article, in addition to contextualizing their earlier optimism about the relations with the United States under President Trump, examines why Chinese leaders and analysts were surprised by the turn of events. It argues that three main factors contributed to the lapse of judgment. First, Chinese officials and analysts grossly misunderstood Donald Trump the individual. By overemphasizing his pragmatism while downplaying his unpredictability, they ended up underprepared for the policies he unleashed. Second, some ingrained Chinese beliefs, manifested in the analogies of the pendulum swing and the ‘bickering couple’, as well as the narrative of the ‘ballast’, lulled officials and scholars into undue optimism about the stability of the broader relationship. Third, analytical and methodological problems as well as political considerations prevented them from fully grasping the strategic shift against China in the US.


Plant Disease ◽  
2013 ◽  
Vol 97 (1) ◽  
pp. 44-52 ◽  
Author(s):  
Vessela Mavrodieva ◽  
Delano James ◽  
Karen Williams ◽  
Sarika Negi ◽  
Aniko Varga ◽  
...  

Four of 19 Prunus germplasm accessions hand carried from the Ukraine into the United States without authorization were found to be infected with Plum pox virus (PPV). Of the three isolates characterized, isolates UKR 44189 and UKR 44191 were confirmed to be isolates of PPV strain W, and UKR 44188 was confirmed to be an isolate of PPV strain D. UKR 44189 and UKR 44191 are very closely related to the PPV strain W isolate LV-145bt (HQ670748) from Latvia. Nucleotide and amino acid sequence identities between these three isolates were greater than 99%. This indicates that the isolates are very closely related and likely originated from a common source. The high genetic diversity among PPV-W strain isolates allowed the identification of potential recombination events between PPV isolates. It appears also that GF 305 peach and Prunus tomentosa are not hosts for the PPV isolate UKR 44189.


1995 ◽  
Vol 8 (1) ◽  
pp. 175-196 ◽  
Author(s):  
Debora Kamrat-Lang

The ArgumentAmerican eugenics developed out of a cultural tradition independent of medicine. However, the eugenicist Harry Hamilton Laughlin and some legal experts involved in eugenic practice in the United States used medical language in discussing and evaluating enforced eugenic sterilizations. They built on medicine as a model for healing, while at the same time playing down medicine's concern with its traditional client: the individual patient. Laughlin's attitude toward medicine was ambivalent because he wanted expert eugenicists, rather than medical experts, to control eugenic practice. In contrast, legal experts saw eugenics as an integral part of medicine, though one expert challenged basing the judicial system on eugenically minded medicine. All in all, the medicalization of American eugenics involved expanding the scope of medicine to include the mutilation of individuals for the benefit of society. The judicial system was medicalized in that an expanded medicine became the basis of legislation in the thirty states that permitted eugenic sterilizations


PEDIATRICS ◽  
1996 ◽  
Vol 97 (2) ◽  
pp. 166-173 ◽  
Author(s):  
Gregory B. Rodgers

Objective. To describe and evaluate the helmet use patterns of children younger than 15 years of age in the United States. Methods. A national telephone survey of bicycle riders was conducted by means of the Mitofsky-Waksberg method of random-digit dialing, a survey method intended to give all telephone numbers in the continental United States an equal probability of selection. Based on information collected in the survey, a logistic regression model was used to determine and quantify the factors associated with helmet use. Results. Information was collected on the bicycle and helmet use patterns of a national sample of 399 children younger than 15 years of age who rode bicycles during the year preceding the survey. This sample projects to the approximately 26.4 million children who are estimated to have ridden bicycles in 1991. About 26% of all child riders owned or had the use of bicycle helmets, and about 15% were reported to have used their helmets all or more than half of the time when riding. Information is provided on the reasons the children did or did not wear helmets. The logistic regression analysis shows that helmet use by children is systematically related to their personal characteristics (eg, age and whether they had previously had bicycle-related accidents requiring medical attention), riding patterns (eg, riding surface), and household demographic characteristics (eg, geographic location and whether household members had attended college). Conclusions. Helmet use rates among children remain low. Less than one fifth of the children who rode bicycles wore helmets all or more than half of the time in 1991. However, based on comparisons with earlier studies, the results of the analysis suggest that helmet use rates have been rising.


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