Mapping for adult-plant resistance against Septoria tritici blotch in a common wheat line Murga

2020 ◽  
Author(s):  
Xinyao He ◽  
Gustavo Azzimonti ◽  
Mariel del Rosario Sánchez-Vidaña ◽  
Silvia Pereyra ◽  
Carolina Sansaloni ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Resistance Mechanism ◽  
Septoria Tritici Blotch ◽  
Septoria Tritici ◽  
Adult Plant ◽  
Wheat Line ◽  
Synthetic Wheat ◽  
Broad Spectrum Resistance ◽  
Fast Development ◽  
The One

Septoria tritici blotch (STB) is a major foliar disease globally, which is notorious in the fast development of fungicide resistance, making host resistance an indispensable component in mitigating STB. CIMMYT wheat line Murga is well known for its high, durable, and broad-spectrum resistance against STB infection, and the purpose of this study was to investigate its resistance mechanism to facilitate its utilization in breeding. A recombinant inbred line population was derived from a cross between Murga and a STB susceptible line Huirivis#1, comprising 297 progenies. The population was evaluated for adult-plant STB resistance in Toluca, Mexico (from 2017 to 2019), and in La Estanzuela, Uruguay (from 2016 to 2018). Genotyping was performed with the DArTSeq platform. QTL mapping indicated a major and stable QTL on chromosome 3DL, explaining a phenotypic variation for STB of 41.2-62.5% in Mexico and 27.5-40.3% in Uruguay. This QTL was regarded as Stb16 based on comparison of its physical position, the possible origin from synthetic wheat, and its broad-spectrum resistance. Additional QTL with minor effects were identified on chromosomes 2B, 2D, 3A, 3B, and 5B. The one on 5BS was significant in four out of the six environments and must be new. Murga was the resistant donor for all QTL, except for those on 2B and 3A. Being an elite breeding line, the Stb16 carrier Murga could be used as a promising STB resistance donor. The rational employment of Stb16 could contribute to STB management yet avoid the rapid emergence of Stb16-virulent isolates.

scholarly journals A wheat cysteine-rich receptor-like kinase confers broad-spectrum resistance against Septoria tritici blotch

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Cyrille Saintenac ◽  
Florence Cambon ◽  
Lamia Aouini ◽  
Els Verstappen ◽  
Seyed Mahmoud Tabib Ghaffary ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Durable Resistance ◽  
Septoria Tritici Blotch ◽  
Septoria Tritici ◽  
Disease Resistance Gene ◽  
Evolutionary Dynamic ◽  
Zymoseptoria Tritici ◽  
Broad Spectrum Resistance ◽  
Receptor Like Kinase ◽  
Synthetic Wheats

AbstractThe poverty of disease resistance gene reservoirs limits the breeding of crops for durable resistance against evolutionary dynamic pathogens. Zymoseptoria tritici which causes Septoria tritici blotch (STB), represents one of the most genetically diverse and devastating wheat pathogens worldwide. No fully virulent Z. tritici isolates against synthetic wheats carrying the major resistant gene Stb16q have been identified. Here, we use comparative genomics, mutagenesis and complementation to identify Stb16q, which confers broad-spectrum resistance against Z. tritici. The Stb16q gene encodes a plasma membrane cysteine-rich receptor-like kinase that was recently introduced into cultivated wheat and which considerably slows penetration and intercellular growth of the pathogen.

scholarly journals New broad-spectrum resistance to septoria tritici blotch derived from synthetic hexaploid wheat

2011 ◽  
Vol 124 (1) ◽  
pp. 125-142 ◽  
Author(s):  
S. Mahmod Tabib Ghaffary ◽  
Justin D. Faris ◽  
Timothy L. Friesen ◽  
Richard G. F. Visser ◽  
Theo A. J. van der Lee ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Hexaploid Wheat ◽  
Septoria Tritici Blotch ◽  
Septoria Tritici ◽  
Synthetic Hexaploid Wheat ◽  
Broad Spectrum Resistance ◽  
Synthetic Hexaploid

scholarly journals QTL Mapping of Seedling and Adult Plant Resistance to Septoria Tritici Blotch in Winter Wheat cv. Mandub (Triticum aestivum L.)

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1108
Author(s):  
Dominika Piaskowska ◽  
Urszula Piechota ◽  
Magdalena Radecka-Janusik ◽  
Paweł Czembor
Keyword(s):  
Mapping Population ◽  
Triticum Aestivum L ◽  
Seedling Stage ◽  
Septoria Tritici Blotch ◽  
Septoria Tritici ◽  
Adult Plant ◽  
Resistance Locus ◽  
Phenotypic Variance ◽  
Breeding Programs ◽  
Plant Stage

Septoria tritici blotch (STB) is one of the most devastating foliar diseases of wheat worldwide. Host resistance is the most economical and safest method of controlling the disease, and information on resistance loci is crucial for effective breeding for resistance programs. In this study we used a mapping population consisting of 126 doubled-haploid lines developed from a cross between the resistant cultivar Mandub and the susceptible cultivar Begra. Three monopycnidiospore isolates of Z. tritici with diverse pathogenicity were used to test the mapping population and parents’ STB resistance at the seedling stage (under a controlled environment) and adult plant stage (polytunnel). For both types of environments, the percentage leaf area covered by necrosis (NEC) and pycnidia (PYC) was determined. A linkage map comprising 5899 DArTSNP and silicoDArT markers was used for the quantitative trait loci (QTL) analysis. The analysis showed five resistance loci on chromosomes 1B, 2B and 5B, four of which were derived from cv. Mandub. The location of QTL detected in our study on chromosomes 1B and 5B may suggest a possible identity or close linkage with Stb2/Stb11/StbWW and Stb1 loci, respectively. QStb.ihar-2B.4 and QStb.ihar-2B.5 detected on chromosome 2B do not co-localize with any known Stb genes. QStb.ihar-2B.4 seems to be a new resistance locus with a moderate effect (explaining 29.3% of NEC and 31.4% of PYC), conferring resistance at the seedling stage. The phenotypic variance explained by QTL detected in cv. Mandub ranged from 11.9% to 70.0%, thus proving that it is a good STB resistance source and can potentially be utilized in breeding programs.

scholarly journals Molecular Mapping of the Stb4 Gene for Resistance to Septoria tritici Blotch in Wheat

2004 ◽  
Vol 94 (11) ◽  
pp. 1198-1206 ◽  
Author(s):  
Tika B. Adhikari ◽  
Jessica R. Cavaletto ◽  
Jorge Dubcovsky ◽  
Jorge Omar Gieco ◽  
Ana Rosa Schlatter ◽  
...  
Keyword(s):  
Molecular Mapping ◽  
Bulked Segregant Analysis ◽  
Recombinant Inbred Lines ◽  
Single Gene ◽  
Effective Means ◽  
Wheat Chromosome ◽  
Septoria Tritici Blotch ◽  
Septoria Tritici ◽  
Adult Plant ◽  
Field Season

Breeding wheat for resistance is the most effective means to control Septoria tritici blotch (STB), caused by the ascomycete Mycosphaerella graminicola (anamorph Septoria tritici). At least eight genes that confer resistance to STB in wheat have been identified. Among them, the Stb4 locus from the wheat cv. Tadinia showed resistance to M. graminicola at both seedling and adult-plant stages. However, no attempt has been made to map the Stb4 locus in the wheat genome. A mapping population of 77 F10 recombinant-inbred lines (RILs) derived from a three-way cross between the resistant cv. Tadinia and the susceptible parent (Yecora Rojo × UC554) was evaluated for disease resistance and molecular mapping. The RILs were tested with Argentina isolate I 89 of M. graminicola for one greenhouse season in Brazil during 1999, with an isolate from Brazil (IPBr1) for one field season in Piracicaba (Brazil) during 2000, and with Indiana tester isolate IN95-Lafayette-1196-WW-1-4 in the greenhouse during 2000 and 2001. The ratio of resistant:susceptible RILs was 1:1 in all three tests, confirming the single-gene model for control of resistance to STB in Tadinia. However, the patterns of resistance and susceptibility were different between the Indiana isolate and those from South America. For example, the ratio of RILs resistant to both the Indiana and Argentina isolates, resistant to one but susceptible to the other, and susceptible to both isolates was approximately 1:1:1:1, indicating that Tadinia may contain at least two genes for resistance to STB. A similar pattern was observed between the Indiana and Brazil isolates. The gene identified with the Indiana tester isolate was assumed to be the same as Stb4, whereas that revealed by the South American isolates may be new. Bulked-segregant analysis was used to identify amplified fragment length polymorphism (AFLP) and microsatellite markers linked to the presumed Stb4 gene. The AFLP marker EcoRI-ACTG/MseI-CAAA5 and microsatellite Xgwm111 were closely linked to the Stb4 locus in coupling at distances of 2.1 and 0.7 centimorgans (cM), respectively. A flanking marker, AFLP EAGG/ M-CAT10, was 4 cM from Stb4. The Stb4 gene was in a potential supercluster of resistance genes near the centromere on the short arm of wheat chromosome 7D that also contained Stb5 plus five previously identified genes for resistance to Russian wheat aphid. The microsatellite marker Xgwm111 identified in this study may be useful for facilitating the transfer of Stb4 into improved cultivars of wheat.

scholarly journals Genome-Wide Association Study Reveals Novel Genetic Loci for Quantitative Resistance to Septoria Tritici Blotch in Wheat (Triticum aestivum L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Tilahun Mekonnen ◽  
Clay H. Sneller ◽  
Teklehaimanot Haileselassie ◽  
Cathrine Ziyomo ◽  
Bekele G. Abeyo ◽  
...  
Keyword(s):  
Genome Wide Association Study ◽  
Agronomic Traits ◽  
Defense Responses ◽  
Septoria Tritici Blotch ◽  
Septoria Tritici ◽  
Adult Plant ◽  
Putative Qtls ◽  
Association Panel ◽  
A Genome ◽  
Trait Associations

Septoria tritici blotch, caused by the fungus Zymoseptoria titici, poses serious and persistent challenges to wheat cultivation in Ethiopia and worldwide. Deploying resistant cultivars is a major component of controlling septoria tritici blotch (STB). Thus, the objective of this study was to elucidate the genomic architecture of STB resistance in an association panel of 178 bread wheat genotypes. The association panel was phenotyped for STB resistance, phenology, yield, and yield-related traits in three locations for 2 years. The panel was also genotyped for single nucleotide polymorphism (SNP) markers using the genotyping-by-sequencing (GBS) method, and a total of 7,776 polymorphic SNPs were used in the subsequent analyses. Marker-trait associations were also computed using a genome association and prediction integrated tool (GAPIT). The study then found that the broad-sense heritability for STB resistance ranged from 0.58 to 0.97 and 0.72 to 0.81 at the individual and across-environment levels, respectively, indicating the presence of STB resistance alleles in the association panel. Population structure and principal component analyses detected two sub-groups with greater degrees of admixture. A linkage disequilibrium (LD) analysis in 338,125 marker pairs also detected the existence of significant (p ≤ 0.01) linkage in 27.6% of the marker pairs. Specifically, in all chromosomes, the LD between SNPs declined within 2.26–105.62 Mbp, with an overall mean of 31.44 Mbp. Furthermore, the association analysis identified 53 loci that were significantly (false discovery rate, FDR, <0.05) associated with STB resistance, further pointing to 33 putative quantitative trait loci (QTLs). Most of these shared similar chromosomes with already published Septoria resistance genes, which were distributed across chromosomes 1B, 1D, 2A, 2B, 2D, 3A,3 B, 3D, 4A, 5A, 5B, 6A, 7A, 7B, and 7D. However, five of the putative QTLs identified on chromosomes 1A, 5D, and 6B appeared to be novel. Dissecting the detected loci on IWGSC RefSeq Annotation v2.1 revealed the existence of disease resistance-associated genes in the identified QTL regions that are involved in plant defense responses. These putative QTLs explained 2.7–13.2% of the total phenotypic variation. Seven of the QTLs (R2 = 2.7–10.8%) for STB resistance also co-localized with marker-trait associations (MTAs) for agronomic traits. Overall, this analysis reported on putative QTLs for adult plant resistance to STB and some important agronomic traits. The reported and novel QTLs have been identified previously, indicating the potential to improve STB resistance by pyramiding QTLs by marker-assisted selection.

scholarly journals Partial Resistance to Septoria Tritici Blotch (Mycosphaerella graminicola) in Wheat Cultivars Arina and Riband

2004 ◽  
Vol 94 (5) ◽  
pp. 497-504 ◽  
Author(s):  
L. Chartrain ◽  
P. A. Brading ◽  
J. P. Widdowson ◽  
J. K. M. Brown
Keyword(s):  
Partial Resistance ◽  
Single Gene ◽  
Heading Date ◽  
Wheat Breeding ◽  
Septoria Tritici Blotch ◽  
Septoria Tritici ◽  
Adult Plant ◽  
Doubled Haploid Population ◽  
Seedling Resistance ◽  
Plant Trials

Partial resistance to Septoria tritici blotch (STB) and its inheritance were investigated in a doubled-haploid population of a cross between cvs. Arina and Riband. The former has good partial resistance whereas the latter is susceptible. In adult plant trials in polytunnels, STB disease scores were negatively correlated with heading date. Resistance was not specific to any of the three fungal isolates used in these tests. A quantitative trait locus (QTL) for partial resistance to STB was identified in Riband on chromosome 6B and is named QStb.psr-6B-1. No QTL controlling a major part of the Arina resistance was identified, suggesting that its resistance may be dispersed and polygenic. There was no correlation between the lines' mean disease scores at the seedling and adult stages, implying that partial resistance to STB is developmentally regulated. Seedling resistance to the isolate IPO323 was isolate-specific and controlled by a single gene in Arina, probably allelic with the Stb6 gene in cv. Flame that confers resistance to the same isolate. The implications of these results for wheat breeding programs are discussed.

scholarly journals Wheat Line “Selection 212” A Source of Broad Spectrum Resistance to Leaf Rust Pathotypes

2019 ◽  
Vol 8 (01) ◽  
pp. 2012-2019 ◽  
Author(s):  
Omkar M. Limbalkar ◽  
J.B. Sharma ◽  
S.K. Jha ◽  
Mandeep Singh ◽  
Pandurang R. Divte ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Broad Spectrum ◽  
Wheat Line ◽  
Broad Spectrum Resistance ◽  
Line Selection

scholarly journals QTL Mapping and Transcriptome Analysis to Identify Differentially Expressed Genes Induced by Septoria Tritici Blotch Disease of Wheat

Agronomy ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 510 ◽  
Author(s):  
Odilbekov ◽  
He ◽  
Armoniené ◽  
Saripella ◽  
Henriksson ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Differentially Expressed Genes ◽  
Resistance Genes ◽  
Transcriptome Analysis ◽  
Resistance Mechanism ◽  
Transcriptome Profiling ◽  
Wheat Breeding ◽  
Differentially Expressed ◽  
Septoria Tritici Blotch ◽  
Septoria Tritici

Resistance to Septoria tritici blotch (STB) is an economically important trait in many wheat-breeding programs across the world. Several quantitative trait loci (QTL) for STB resistance were identified in wheat but due to the dynamic pathogen population it is necessary to continuously identify new resistance genes/QTL and determine the underlying resistance mechanism. In this work, we integrated QTL mapping and transcriptome profiling to identify candidate genes underlying QTL associated with STB resistance in bread wheat at the seedling stage. The results revealed four QTL on chromosomes 1BS, 1BL, 3AS and 3DL for STB resistance. Among these, two QTL on 2BL and 3DL were mapped for chlorosis, necrosis and pycnidia while the other two on 1BS and 3AS were associated with necrosis and pycnidia. Among the four identified QTL, genes were identified in three QTL (1BS, 2BL and 3DL). In total, 238 differentially expressed genes (DEGs) were localized in 1BS, 16 DEGs in 2BL and 80 DEGs in 3DL QTL region respectively. F-box protein, NBS-LRR disease resistance genes and receptor-like protein kinase were the most over-represented. The results emphasize the importance of integrating QTL and transcriptome analysis to accelerate the identification of key genes underlying the traits of interest.

scholarly journals Additional Sources of Broad-Spectrum Resistance to Puccinia coronata f. sp. avenae from Canadian Accessions of Avena barbata

Plant Disease ◽  
2010 ◽  
Vol 94 (12) ◽  
pp. 1405-1410 ◽  
Author(s):  
M. L. Carson
Keyword(s):  
United States ◽  
Broad Spectrum ◽  
Rust Resistance ◽  
The United States ◽  
Crown Rust ◽  
Puccinia Coronata ◽  
Adult Plant ◽  
United States Department ◽  
Ploidy Levels ◽  
Broad Spectrum Resistance

Crown rust (Puccinia coronata f. sp. avenae) is considered the most damaging disease of oat and the use of race-specific seedling (Pc) genes for resistance has been the primary means of control. As these resistance genes from cultivated oat, Avena sativa, and the wild hexaploid animated oat, A. sterilis, were deployed in oat cultivars, corresponding virulence in the U.S. crown rust population increased rapidly, such that the effective lifespan of a resistant cultivar in the United States is now 5 years or less. Introgression of resistance from diploid and tetraploid Avena spp. into hexaploid oat has been difficult due to the difference in ploidy levels and the lack of pairing of homeologous chromosomes between species. The wild tetraploid slender oat, A. barbata, has been a source of powdery mildew and stem rust resistance in cultivated oat but has largely been unexploited for crown rust resistance. A relatively high percentage of A. barbata accessions from the United States Department of Agriculture (USDA) National Small Grains Collection were resistant to a highly diverse crown rust population in recent tests. Tests of 1,099 A. barbata accessions from the Canadian Plant Gene Resources Center not represented in the USDA collection revealed that a similar percentage (11.4%) were at least moderately resistant at the seedling and adult plant stage when tested with a highly diverse bulk inoculum derived from the St. Paul buckthorn nursery. Eighteen accessions were rated as highly resistant or a mix of highly resistant and resistant plants in both seedling and adult plant tests. Three accessions (CN21531 from Italy and CN26271 and CN26305 from Spain) displayed a unique “blotchy” resistant reaction as adult plants. Resistant accessions were found from throughout much of the natural range of A. barbata but the Western Mediterranean and Lebanon had the highest frequency of accessions with broad-spectrum resistance.

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