scholarly journals Involvement of Beet western yellows virus, Cauliflower mosaic virus, and Turnip mosaic virus in Internal Disorders of Stored White Cabbage

2002 ◽  
Vol 92 (8) ◽  
pp. 816-826 ◽  
Author(s):  
P. J. Hunter ◽  
J. E. Jones ◽  
J. A. Walsh
Keyword(s):  
Mosaic Virus ◽  
Leaf Tissue ◽  
Subsequent Development ◽  
Turnip Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
Beet Western Yellows Virus ◽  
Growing Seasons ◽  
White Cabbage ◽  
Necrotic Spots ◽  
Internal Necrosis

Experiments over two growing seasons clearly showed that Turnip mosaic virus (TuMV) infection was associated with internal necrosis (sunken necrotic spots 5 to 10 mm in diameter) and Beet western yellows virus (BWYV) infection was associated with collapse of leaf tissue at the margins (tipburn) in heads of stored white cabbage (Brassica oleracea var. capitata). Virtually no tipburn was seen in cv. Polinius, whereas cv. Impala was affected severely. Internal necrotic spots were seen in both cultivars. BWYV appeared to interact with TuMV. Plants infected with both viruses showed a lower incidence of external symptoms and had less internal necrosis than plants infected with TuMV alone. Cauliflower mosaic virus (CaMV) did not induce significant amounts of internal necrosis or tipburn, but did, in most cases, exacerbate symptoms caused by TuMV and BWYV. BWYV-induced tipburn worsened significantly during storage. Post-transplanting inoculation with TuMV induced more internal necrosis than pre-transplant inoculation. There was a significant association between detection of TuMV just prior to harvest and subsequent development of internal necrotic spots. Individually, all three viruses significantly reduced the yield of cv. Polinius, whereas only BWYV and CaMV treatments reduced the yield of cv. Impala.

scholarly journals Threat of Turnip mosaic virus strains to rare and endangered native Lepidium spp in the South Island New Zealand

2011 ◽  
Vol 64 ◽  
pp. 289-289 ◽  
Author(s):  
J.D. Fletcher ◽  
S.R. Bulman ◽  
J. Van_Vianen ◽  
P.B. Heenan ◽  
G.J. Houliston
Keyword(s):  
New Zealand ◽  
Mosaic Virus ◽  
Turnip Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
The South ◽  
Beet Western Yellows Virus ◽  
Pcr Products ◽  
Rare And Endangered ◽  
Survey Results ◽  
Comparative Phylogenetic Analysis

Cooks scurvy grass (Lepidium oleraceum agg) is an endangered species of native Brassicaceae that is considered threatened by extinction Viruslike disease symptoms were observed in a newly introduced plant of L oleraceum at Stony Bay Banks Peninsula Canterbury New Zealand in 2008 Turnip mosaic virus (TuMV) was subsequently confirmed as the cause of symptoms A survey was undertaken at seven isolated South Island sites where L oleraceum and other Lepidium species were growing TuMV was detected in around 20 of plants at two sites Cauliflower mosaic virus (CaMV) was also detected at three sites with up to 50 incidence at one site and there was some evidence of a Beet western yellows virus (BWYV) infection Further isolates of TuMV were also collected from commercial brassica crops in the South Island RT PCR products for all of the virusinfected plant material were sequenced analysed phylogenetically and compared This poster reports on survey results and the comparative phylogenetic analysis of the TuMV isolates

Internal necrosis in stored white cabbage caused by turnip mosaic virus

1978 ◽  
Vol 89 (3) ◽  
pp. 435-441 ◽  
Author(s):  
D. G. A. WALKEY ◽  
M. J. W. WEBB
Keyword(s):  
Mosaic Virus ◽  
Turnip Mosaic Virus ◽  
White Cabbage ◽  
Internal Necrosis

scholarly journals The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia

2016 ◽  
Vol 31 (1-2) ◽  
pp. 37-44 ◽  
Author(s):  
Dragana Milosevic ◽  
Maja Ignjatov ◽  
Zorica Nikolic ◽  
Ivana Stankovic ◽  
Aleksandra Bulajic ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Oilseed Rape ◽  
Sequence Data ◽  
Cauliflower Mosaic Virus ◽  
Rt Pcr ◽  
Brassica Napus L ◽  
Beet Western Yellows Virus ◽  
Turnip Yellows Virus ◽  
Test Plants ◽  
Das Elisa

A total of 86 oilseed rape samples from six crops in different localities were collected during 2014 and analyzed for the presence of Turnip yellows virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was serologically detected in 60 collected samples (69.77%), and none of the samples tested were positive for CaMV and TuMV. Six selected TuYV isolates were successfully transmitted by Myzus persicae to three test plants, confirming the infectious nature of the disease. In the selected ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR and sequencing. A comparison of the obtained sequence with those available in GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis of P0 gene sequence data for a subset of these isolates showed they clustered with the known TuYV and were distinct from Beet western yellows virus (BWYV) isolates.

scholarly journals Temporal and Spatial Appearance of Recombinant Viruses Formed Between Cauliflower Mosaic Virus (CaMV) and CaMV Sequences Present in Transgenic Nicotiana bigelovii

1998 ◽  
Vol 11 (4) ◽  
pp. 309-316 ◽  
Author(s):  
Lorant Király ◽  
June E. Bourque ◽  
James E. Schoelz
Keyword(s):  
Transgenic Plants ◽  
Mosaic Virus ◽  
Leaf Tissue ◽  
Cauliflower Mosaic Virus ◽  
Recombinant Viruses ◽  
Predominant Type ◽  
Nicotiana Bigelovii ◽  
Temporal And Spatial ◽  
Systemic Symptoms ◽  
Symptom Type

Cauliflower mosaic virus (CaMV) strain CM1841 is able to recombine with a CaMV transgene sequence present in Nicotiana bigelovii. In the present study we have characterized the temporal and spatial appearance of recombinant viruses formed between CM1841 and the transgene within individual transgenic plants. CM1841 infections were initiated by mechanical inoculation and by agro-inoculation to nontransformed N. bigelovii and transgenic N. bigelovii that expressed the gene VI product of CaMV strain D4. In agroinoculated transgenic plants, inoculated leaf tissue turned necrotic around the point of agroinocu-lation, while chlorotic lesions appeared in the leaves inoculated with CM1841 virions. The first systemic symptoms in both agroinoculated and mechanically inoculated transgenic N. bigelovii consisted of necrotic patches. The predominant type of virus recovered from the inoculated and first systemically infected leaves was the wild-type CM1841 rather than a recombinant. As the infection progressed in the transgenic plants, symptoms changed from necrosis in the lower leaves to a chlorotic mosaic in the upper leaves. This shift in symptom type was associated with the recovery of recombinant viruses, indicating that the recombinants predominated only in later stages of pathogenesis.

scholarly journals First Report of Turnip ringspot virus in Field Mustard (Brassica chinensis) in Taiwan

Plant Disease ◽  
2011 ◽  
Vol 95 (8) ◽  
pp. 1036-1036
Author(s):  
Y.-K. Chen ◽  
Y.-S. Chang ◽  
H.-J. Bau
Keyword(s):  
Amino Acids ◽  
Mosaic Virus ◽  
Amino Acid Sequences ◽  
Cauliflower Mosaic Virus ◽  
Ringspot Virus ◽  
Coat Proteins ◽  
Degenerate Primers ◽  
Brassica Chinensis ◽  
First Report ◽  
Beet Western Yellows Virus

Crucifer crops (Brassica spp.) are important winter vegetables in Taiwan. Five viruses, including Turnip mosaic virus (TuMV), Cucumber mosaic virus (CMV), Radish mosaic virus (RaMV), Beet western yellows virus (BWYV), and Cauliflower mosaic virus (CaMV), have been detected in a range of domestic-grown crucifers during past decades (1). Field mustard plants (Brassica chinensis) showing mosaic in the leaves were collected in the ChiaYi area in December 2007. Spherical virus-like particles, approximately 30 nm in diameter, were readily observed in crude sap of symptomatic plants. Tests by ELISA failed to detect any of the aforementioned viruses. A spherical agent was isolated through mechanical inoculation onto Chenopodium quinoa, and a virus culture was established and inoculated mechanically back to the original host as well as other crucifers. Systemic mosaic appeared on inoculated B. campestris, B. chinensis, and B. juncea, whereas ringspots appeared on inoculated leaves of B. oleracea. Total RNA was extracted from symptomatic leaves and used for reverse transcription (RT)-PCR amplification using degenerate primers for comoviruses (2). Other successive fragments of RNAs 1 and 2 were amplified by specific or degenerate primers designed on the basis of sequences of published Turnip ringspot virus (TuRSV). The RNA 1 (GenBank Accession No. GU968732) and RNA 2 (No. GU968731) of the isolated virus consisted of 6,076 and 3,960 nucleotides, respectively. The number of nucleotides and the arrangement of open reading frames on both RNA 1 and RNA 2 were similar to those of comoviruses. Sequence analysis revealed that the nucleotide sequences of RNA 1 and RNA 2 shared 54.2 to 82.5% and 50.2 to 79.3% similarities, respectively, to those of comoviruses and were most similar to Turnip ringspot virus. The deduced peptides of large and small coat proteins (LCP and SCP) contain 375 amino acids (41.2 kDa) and 251 amino acids (28.5 kDa), respectively. The deduced amino acid sequences of RNA-dependent RNA polymerase (RdRp), LCP, and SCP share 92.0 to 94.5%, 93.1 to 93.3% and 87.3 to 89.6% similarity, respectively, to those of published TuRSV isolates, i.e., -B (GenBank Accession No. GQ222382), -M12 (No. FJ516746), and -Toledo (No. FJ712027) indicating that the newly isolated virus from field mustard in Taiwan is an isolate of TuRSV, hence TuRSV-TW. Comparison of LCP and SCP between current TuRSV-TW and Radish mosaic virus (RaMV; GenBank Accession No. AB295644) showed 74% similarity, which is below the species demarcation level of 75% (3), indicating its discrimination from RaMV. To our knowledge, this is the first report of the occurrence of TuRSV in Taiwan and in the subtropics. References: (1) T. H. Chen et al. Plant Pathol. Bull. 9:39, 2000. (2) V. Maliogka et al. J. Phytopathol. 152:404, 2004. (3) K. Petrzik and I. Koloniuk. Virus Genes 40:290, 2010.

Resistance to turnip mosaic virus in white cabbage

Euphytica ◽  
1990 ◽  
Vol 51 (2) ◽  
pp. 101-107
Author(s):  
D. A. C. Pink ◽  
D. G. A. Walkey
Keyword(s):  
Mosaic Virus ◽  
Turnip Mosaic Virus ◽  
White Cabbage

Economic impact of Turnip mosaic virus, Cauliflower mosaic virus and Beet mosaic virus in three Kenyan vegetables

2007 ◽  
Vol 56 (2) ◽  
pp. 317-323 ◽  
Author(s):  
N. J. Spence ◽  
N. A. Phiri ◽  
S. L. Hughes ◽  
A. Mwaniki ◽  
S. Simons ◽  
...  
Keyword(s):  
Economic Impact ◽  
Mosaic Virus ◽  
Turnip Mosaic Virus ◽  
Cauliflower Mosaic Virus

scholarly journals Pharmacological analysis of transmission activation of two aphid-vectored plant viruses, turnip mosaic virus and cauliflower mosaic virus

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Edwige Berthelot ◽  
Jean-Luc Macia ◽  
Alexandre Martinière ◽  
Alexandre Morisset ◽  
Romain Gallet ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Plant Viruses ◽  
Turnip Mosaic Virus ◽  
Cauliflower Mosaic Virus ◽  
Pharmacological Analysis

scholarly journals DETEKSI TURNIP MOSAIC VIRUS PADA JARINGAN BENIH DAN DAUN

2006 ◽  
Vol 6 (1) ◽  
pp. 32-40
Author(s):  
Kartiningtyas Kartiningtyas ◽  
Sri Hendrastuti Hidayat
Keyword(s):  
Mosaic Virus ◽  
Leaf Tissue ◽  
Seed Transmission ◽  
Transmission Efficiency ◽  
Turnip Mosaic Virus ◽  
Rt Pcr ◽  
Detection Techniques ◽  
Central Java ◽  
Time Of Infection ◽  
Pcr Techniques

Detection of Turnip mosaic virus in seed and leaf tissue. The study was conducted to test the seed transmission efficiency of Turnip mosaic virus (TuMV) on caisin (Brassica rapa) and the susceptibility of plant to the virus at different ages.  Two detection techniques, ELISA and RT-PCR,  were used to determine the more appropriate method for detection of TuMV. Two different sources of seeds involved those from farmer and commercial seeds were collected from West Java and Central Java.   TuMV was inoculated on test plants at 2, 4, 6, 8, and 10 weeks after transplanting.  Infected plants were confirmed using ELISA and RT-PCR techniques with specific antiserum and primer.  TuMV was detected from farmer seeds originated from Ciherang and Cinangneng with percent infection of 15% and 2% , respectively.  Plant growth and symptom development were affected by time of infection.  In general, TuMV infection caused symptoms, mosaic, malformation, vein clearing, and blister on the leaf.  The youngest plants were more susseptble and shown more severe symptoms.  Absorbent value of ELISA from infected plants was in the range of 2.1 – 2.4.  Spesific DNA band, 800 bp, was amplified from infected plants. 

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