scholarly journals A low cost electromechanical device injury model for hind limb murine muscles

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Daniel Perez-Hernandez ◽  
Bertha Segura-Alegria ◽  
Karla Garcia-Pelagio
Keyword(s):  
Hind Limb ◽  
Low Cost ◽  
Injury Model ◽  
Electromechanical Device

In Vitro Characterization of Umbilical Cord Blood CD133+ and In Vivo Functionality in Response to Ischemia in a Murine Hind-Limb Injury Model.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3693-3693
Author(s):  
Marcie R. Finney ◽  
Nicholas G. Greco ◽  
Matthew E. Joseph ◽  
Daniel G. Winter ◽  
Shyam Bhakta ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Hind Limb ◽  
Study Groups ◽  
Chemotactic Migration ◽  
Limb Injury ◽  
Injury Model

Abstract Previous reports have demonstrated efficacy of cellular therapy in mediating therapeutic angiogenesis in response to ischemia. We sought to determine the potential efficacy of adult umbilical cord blood (UCB) derived selected CD133+ cells in the murine hind limb ischemia model and to characterize these cells by surface phenotype and functionality prior to injection. Methods and Results: Mononuclear cells (MNC) from UCB were labeled with CD133+ conjugated magnetic beads, followed by automated sorting through magnetic columns (Miltenyi). Routine yield of CD133+ cells was 0.51 ± 0.2% of MNC, with a purity of 78.7 ± 2.4% (n=30). Surface expression in the UCB CD133+ population was 3.59 ± 1.49% KDR(VEGFR2), 8.66 ± 3.79% CXCR4 and 22.74 ± 2.84% CD105 compared to 7.14 ± 2.15% KDR, 28.54 ± 5.81% CXCR4 and 6.74 ± 2.07% CD105 in the UCB MNC population. Transwell plates with 5μm collagen coated filters (Costar) were used to observe chemotactic migration of MNC or CD133+ cells towards SDF-1 (100ng/mL) compared to control wells containing media alone. Following a 3 hour incubation, the cells migrating to the bottom wells were counted by flow cytometry with TruCOUNT™ tubes (BD Biosciences). MNC and CD133+ cells migration to SDF showed a 4.9 ± 2.9 and 1.8 ± 0.7 fold increase over the negative control respectively. To test vasculogenic functionality of these selected cell populations, NOD/SCID mice underwent ligation of the right femoral artery and were randomized into 3 study groups: control (endothelial media with cytokines), non-selected MNC (1 x 106 cells/mouse) or CD133+ (0.5 x 106 cells/mouse) given via intracardiac injection immediately after injury. Doppler flow measurements were taken on both limbs each week for 4 weeks and the ratio of perfusion in the ischemic/healthy limb was calculated. At 28 days, perfusion ratios were statistically higher in study groups receiving CD133+ cells from UCB, 0.55 ± 0.07 (n=8) compared to cytokine controls 0.39 ± 0.02 (n=10, p=0.019). Mice receiving MNC did not show statistically significant improvement over control animals 0.42 ± 0.06 (n=7, p=0.27). Conclusion: Surface phenotyping was notable for increased expression of the receptor for SDF-1, CXCR4 on MNC when compared to CD133+ cells. In vitro functional assays showed that CD133+ and MNC exhibited increased chemotactic migration to SDF-1. In vivo studies showed that injection of UCB CD133+ cells improved blood flow compared with cytokines alone in the murine hind limb injury model, highlighting the vasculogenic potential of CD133+ cells from UCB.

scholarly journals Effects of C1 inhibitor on endothelial cell activation in a rat hind limb ischemia-reperfusion injury model

2018 ◽  
Vol 68 (6) ◽  
pp. 209S-221S.e2 ◽  
Author(s):  
Shengye Zhang ◽  
Jane Shaw-Boden ◽  
Yara Banz ◽  
Anjan K. Bongoni ◽  
Adriano Taddeo ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Reperfusion Injury ◽  
Hind Limb ◽  
Cell Activation ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Limb Ischemia ◽  
C1 Inhibitor ◽  
Endothelial Cell Activation ◽  
Injury Model

Human embryonic stem cell-derived mesenchymal stromal cell transplantation in a rat hind limb injury model

Cytotherapy ◽  
2009 ◽  
pp. 1-12
Author(s):  
Juha Laurila ◽  
Lilja Laatikainen ◽  
Maria Castellone ◽  
Parul Trivedi ◽  
Jari Heikkila ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Transplantation ◽  
Mesenchymal Stromal Cell ◽  
Hind Limb ◽  
Human Embryonic Stem Cell ◽  
Stromal Cell ◽  
Embryonic Stem ◽  
Limb Injury ◽  
Injury Model

scholarly journals Schisandrin B Prevents Hind Limb from Ischemia-Reperfusion-Induced Oxidative Stress and Inflammation via MAPK/NF-κB Pathways in Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Ning Zhu ◽  
Changhong Cai ◽  
Aiming Zhou ◽  
Xuyong Zhao ◽  
Yijia Xiang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Hind Limb ◽  
Ischemia Reperfusion ◽  
Limb Ischemia ◽  
Schisandrin B ◽  
Sod Activity ◽  
Neuroprotective Effects ◽  
Beneficial Effects ◽  
Injury Model

Schisandrin B (ScB), isolated from Schisandra chinensis (S. chinensis), is a traditional Chinese medicine with proven cardioprotective and neuroprotective effects. However, it is unclear whether ScB also has beneficial effects on rat hind limb ischemia/reperfusion (I/R) injury model. In this study, ScB (20 mg/kg, 40 mg/kg, and 80 mg/kg) was administered via oral gavage once daily for 5 days before the surgery. After 6 h ischemia and 24 h reperfusion of left hind limb, ScB reduced I/R induced histological changes and edema. ScB also suppressed the oxidative stress through decreasing MDA level and increasing SOD activity. Moreover, above changes were associated with downregulated TNF-α mRNA expression and reduced level of IL-1β in plasma. Meanwhile, ScB treatment downregulated activation of p38MAPK, ERK1/2, and NF-κB in ischemic skeletal muscle. These results demonstrate that ScB treatment could prevent hind limb I/R skeletal muscle injury possibly by attenuating oxidative stress and inflammation via p38MAPK, ERK1/2, and NF-κB pathways.

scholarly journals Bletilla striataMicron Particles Function as a Hemostatic Agent by Promoting Rapid Blood Aggregation

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Chen Zhang ◽  
Rui Zeng ◽  
Zhencheng Liao ◽  
Chaomei Fu ◽  
Hui Luo ◽  
...  
Keyword(s):  
Low Cost ◽  
In Vivo Studies ◽  
Hemostatic Agent ◽  
Severe Bleeding ◽  
Promising Alternative ◽  
Bletilla Striata ◽  
Visible Particle ◽  
Injury Model

The human body cannot control blood loss without treatment. Available hemostatic agents are ineffective at treating cases of severe bleeding and are expensive or raise safety concerns.Bletilla striataserve as an inexpensive, natural, and promising alternative. However, no detailed studies on its hemostatic approach have been performed. The aim of this study was to examine the hemostatic effects ofB. striataMicron Particles (BSMPs) and their hemostatic mechanisms. We prepared and characterized BSMPs of different size ranges and investigated their use as hemostatic agent. BSMPs of different size ranges were characterized by scanning electron microscope. In vitro coagulation studies revealed BSMP-blood aggregate formation via stereoscope and texture analyzers. In vivo studies based on rat injury model illustrated the BSMP capabilities under conditions of hemostasis. Compared to other BSMPs of different size ranges, BSMPs of 350–250 μm are most efficient in hemostasis. As powder sizes decrease, the degree of aggregation between particles and hemostatic BSMP effects declines. The BSMP in contact with a bleeding surface locally forms a visible particle/blood aggregate as a physical barrier that facilitates hemostasis. Considering the facile preparation, low cost, and long shelf life ofB. striata, BSMPs offer great potential as mechanisms of trauma treatment.

Recovery from hind limb ischemia enhances rhBMP-2-mediated segmental bone defect repair in a rat composite injury model

Bone ◽  
2013 ◽  
Vol 55 (2) ◽  
pp. 410-417 ◽  
Author(s):  
Brent A. Uhrig ◽  
Joel D. Boerckel ◽  
Nick J. Willett ◽  
Mon-Tzu A. Li ◽  
Nathaniel Huebsch ◽  
...  
Keyword(s):  
Bone Defect ◽  
Hind Limb ◽  
Limb Ischemia ◽  
Segmental Bone Defect ◽  
Hind Limb Ischemia ◽  
Bone Defect Repair ◽  
Injury Model ◽  
Defect Repair ◽  
Segmental Bone

scholarly journals Human embryonic stem cell-derived mesenchymal stromal cell transplantation in a rat hind limb injury model

Cytotherapy ◽  
2009 ◽  
Vol 11 (6) ◽  
pp. 726-737 ◽  
Author(s):  
Juha P. Laurila ◽  
Lilja Laatikainen ◽  
Maria D. Castellone ◽  
Parul Trivedi ◽  
Jari Heikkila ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Transplantation ◽  
Mesenchymal Stromal Cell ◽  
Hind Limb ◽  
Human Embryonic Stem Cell ◽  
Stromal Cell ◽  
Embryonic Stem ◽  
Limb Injury ◽  
Injury Model

Design and Synthesis of an Aniline Derivative with Biological Activity on Heart Failure

2020 ◽  
Vol 23 ◽  
Author(s):  
Garcimarero-Espino E. Alejandra ◽  
Figueroa-Valverde Lauro ◽  
Rosas-Nexticapa Marcela ◽  
Lopez-Ramos Maria ◽  
Diaz Cedillo Francisco ◽  
...  
Keyword(s):  
Heart Failure ◽  
Biological Activity ◽  
Ischemia Reperfusion Injury ◽  
Simple Procedure ◽  
Low Cost ◽  
Ischemia Reperfusion ◽  
Aniline Derivative ◽  
Design And Synthesis ◽  
Aniline Derivatives ◽  
Injury Model

Background: Several compounds have been synthesized as a therapeutic alternative for heart failure; however, its preparation requires special conditions. Objective: The aim of this study, was to synthesize some aniline derivatives (4-9) from 3-ethynylaniline to evaluate their biological activity against heart failure. Methods: The synthesis of aniline derivatives involved a series of reactions such as etherification, addition, and cyclization. The structure of all compounds obtained was confirmed by spectroscopic and spectrometric methods. In addition, to evaluate the biological activity of compounds an ischemia/reperfusion injury model was used. Results: The results showed that compound 8 decreases heart failure, which translates into a decrease in the infarction area compared to compounds 4-7 and 9. Conclusion: This study is reported a facile method for the preparation of aniline derivatives. This method offers some advantages such as; a simple procedure, low cost, and ease of work. In addition, compound 8 showed an interesting biological activity against heart failure. This phenomenon is particularly interesting because the biological activity induced by this compound could involve a molecular mechanism different compared with other drugs used for the treatment of heart failure.

Decomposition of the metastable beta titanium alloy Ti-15-3

1983 ◽  
Vol 41 ◽  
pp. 264-265
Author(s):  
Y. L. Chen ◽  
S. Fujlshiro
Keyword(s):  
Low Cost ◽  
High Strength ◽  
Alpha Phase ◽  
Thick Sheet ◽  
Omega Phase ◽  
Beta Titanium Alloy ◽  
Beta Titanium ◽  
Beta Matrix ◽  
Metastable Beta ◽  
Very High

Metastable beta titanium alloys have been known to have numerous advantages such as cold formability, high strength, good fracture resistance, deep hardenability, and cost effectiveness. Very high strength is obtainable by precipitation of the hexagonal alpha phase in a bcc beta matrix in these alloys. Precipitation hardening in the metastable beta alloys may also result from the formation of transition phases such as omega phase. Ti-15-3 (Ti-15V- 3Cr-3Al-3Sn) has been developed recently by TIMET and USAF for low cost sheet metal applications. The purpose of the present study was to examine the aging characteristics in this alloy.The composition of the as-received material is: 14.7 V, 3.14 Cr, 3.05 Al, 2.26 Sn, and 0.145 Fe. The beta transus temperature as determined by optical metallographic method was about 770°C. Specimen coupons were prepared from a mill-annealed 1.2 mm thick sheet, and solution treated at 827°C for 2 hr in argon, then water quenched. Aging was also done in argon at temperatures ranging from 316 to 616°C for various times.

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