Interaction in the interface between domain 3 (D3) and domain 4 (D4) regulate the conformational changes in type IA topoisomerases

This study aimed to evaluate the general quality of life (QoL) of Brazilian vegetarians. A cross-sectional study was conducted with Brazilian vegetarian adults (18 years old and above). Individuals were recruited to participate in a nationwide online survey that comprised the WHOQOL-BREF as well as sociodemographic and characterization questions related to vegetarianism. The WHOQOL-BREF is composed of 24 items which are divided into four domains (domain 1: physical health; domain 2: psychological well-being; domain 3: social relationships; and domain 4: environment), plus two general items which were analyzed separately, totaling 26 items. The answers from the questionnaire were converted into scores with a 0–100 scale range, with separate analyses for each domain. Results were compared among groups based on the different characteristics of the vegetarian population. A total of 4375 individuals completed the survey. General average score results were 74.67 (domain 1), 66.71 (domain 2), 63.66 (domain 3) and 65.76 (domain 4). Vegans showed better scores when compared to the other vegetarians, except in domain four, where the statistical difference was observed only for semi-vegetarians (lower score). Individuals adopting a vegetarian diet for longer (>1 year) showed better results for domains one and two, with no difference for the other domains. Having close people also adopting a vegetarian diet positively influenced the results for all domains. On the other hand, it was not possible to distinguish any clear influence of the motivation for adopting a vegetarian diet on the scores’ results. Adopting a vegetarian diet does not have detrimental effects on one’s QoL. In fact, the more plant-based the diet, and the longer it was adopted, the better the results were.

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The glucose-6-phosphatase system

Biochemical Journal ◽

10.1042/bj3620513 ◽

2002 ◽

Vol 362 (3) ◽

pp. 513-532 ◽

Cited By ~ 170

Author(s):

Emile van SCHAFTINGEN ◽

Isabelle GERIN

Keyword(s):

Endoplasmic Reticulum ◽

Conformational Changes ◽

Transport Model ◽

Glycogen Storage Disease Type ◽

Glycogen Storage ◽

Water Soluble ◽

Gene Encoding ◽

Genes Encoding ◽

Type Ia ◽

Micro Organisms

Glucose-6-phosphatase (G6Pase), an enzyme found mainly in the liver and the kidneys, plays the important role of providing glucose during starvation. Unlike most phosphatases acting on water-soluble compounds, it is a membrane-bound enzyme, being associated with the endoplasmic reticulum. In 1975, W. Arion and co-workers proposed a model according to which G6Pase was thought to be a rather unspecific phosphatase, with its catalytic site oriented towards the lumen of the endoplasmic reticulum [Arion, Wallin, Lange and Ballas (1975) Mol. Cell. Biochem. 6, 75–83]. Substrate would be provided to this enzyme by a translocase that is specific for glucose 6-phosphate, thereby accounting for the specificity of the phosphatase for glucose 6-phosphate in intact microsomes. Distinct transporters would allow inorganic phosphate and glucose to leave the vesicles. At variance with this substrate-transport model, other models propose that conformational changes play an important role in the properties of G6Pase. The last 10 years have witnessed important progress in our knowledge of the glucose 6-phosphate hydrolysis system. The genes encoding G6Pase and the glucose 6-phosphate translocase have been cloned and shown to be mutated in glycogen storage disease type Ia and type Ib respectively. The gene encoding a G6Pase-related protein, expressed specifically in pancreatic islets, has also been cloned. Specific potent inhibitors of G6Pase and of the glucose 6-phosphate translocase have been synthesized or isolated from micro-organisms. These as well as other findings support the model initially proposed by Arion. Much progress has also been made with regard to the regulation of the expression of G6Pase by insulin, glucocorticoids, cAMP and glucose.

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Pengaruh Konseling Home care Terhadap Kualitas Hidup Penderita DM Tipe 2 Di Puskesmas Talise Kota Palu

Preventif : Jurnal Kesehatan Masyarakat ◽

10.22487/preventif.v12i2.449 ◽

2021 ◽

Vol 12 (2) ◽

pp. 332

Author(s):

Nur Hikmah Buchair ◽

Ridwan Amiruddin ◽

Indar Indar

Keyword(s):

Diabetes Mellitus ◽

Home Care ◽

Domain 3 ◽

Diabetes Melitus ◽

Domain 4

Penderita Diabetes Melitus umumnya memiliki kualitas hidup yang buruk dibandingkan dengan orang yang tanpa penyakit sehingga membutuhkan perawatan diabetes yang tepat melalui intervensi perilaku berupa dukungan pendidikan dan konseling untuk mengaktifkan penderita diabetes untuk mengelola penyakitnya. Penelitian ini bertujuan untuk mengetahui pengaruh konseling dalam home care terhadap kualitas hidup penderita Diabetes Mellitus Tipe 2. Penelitian ini dilaksanakan di wilayah kerja Puskesmas Talise dan wilayah kerja Puskesmas Birobuli Kota Palu. Desain penelitian adalah Eksperimen Semu dengan jumlah sampel sebanyak 40 responden yang dipilih secara acak. Analisis data menggunakan Uji Beda Dua Mean yaitu Uji Wilcoxon dan Uji Beda Dua Mean Independen yaitu Uji Mann Whitney. Hasil penelitian menunjukkan ada pengaruh konseling home care terhadap kualitas hidup penderita DM baik pada Domain 1 (Kesehatan Fisik), Domain 2 (Kondisi Psikologis), Domain 3 (Hubungan Sosial), Domain 4 (Lingkungan) dimana skor rata- rata (mean) sebelum intervensi adalah 60,55 dengan standar deviasi 2,605 meningkat menjadi 71,35 dengan standar deviasi 4,107 setelah intervensi. Secara statistik terjadi perbedaan kualitas hidup sebelum dan setelah diberikan konseling dengan nilai p= 0,000 (p<0,05) yang berarti bahwa konseling home care efektif diberikan pada penderita DM untuk meningkatkan kualitas hidup penderita DM.

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Crystal structures of Sulfolobus solfataricus topoisomerase III reveal that its C-terminal novel zinc finger part is a unique decatenation domain

10.1101/706986 ◽

2019 ◽

Author(s):

Hanqian Wang ◽

Junhua Zhang ◽

Xin Zheng ◽

ZhenFeng Zhang ◽

Zhiyong Zhang ◽

...

Keyword(s):

Crystal Structures ◽

Zinc Finger ◽

Conformational Changes ◽

Sulfolobus Solfataricus ◽

Dna Topoisomerases ◽

Cellular Processes ◽

Topoisomerase Iii ◽

Type Ia ◽

Strand Passage ◽

Domain V

AbstractDNA topoisomerases are essential enzymes for a variety of cellular processes involved in DNA transactions. Many of the mechanistic insights into type IA DNA topoisomerases have principally come from studies on the prokaryotes and eukaryotes. However, a structural understanding of type IA topoisomerases in the Archaeal is lacking. Here we report the crystal structures of full-length Sulfolobus solfataricus topoisomerase III (Sso topo III) both by itself and in complex with an 8-base single-stranded DNA fragment, which were determined at 2.1 Å and 2.5 Å, respectively. The structures show that, as a member of type IA topoisomerases, Sso topo III adopts a torus-like architecture consisting of a four-domain core region and a novel C-terminal zinc finger domain (domain V). Upon binding to ssDNA, Sso topo III undergoes dramatic conformational changes, similar to those of other type IA topoisomerases. Structural analyses and biochemical assays revealed that domain V is essential for the DNA decatenation activity of Sso topo III. These findings establish Sso topo III as an alternative prototype of type IA topoisomerases to further understand the loop-independent decatenation mechanism in the enzyme-bridged strand passage model.

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Direct Observation of Topoisomerase IA Gate Dynamics

10.1101/356659 ◽

2018 ◽

Author(s):

Maria Mills ◽

Yuk-Ching Tse-Dinh ◽

Keir C. Neuman

Keyword(s):

Single Molecule ◽

Conformational Changes ◽

Mechanistic Model ◽

Cellular Functions ◽

Single Stranded Dna ◽

Gate Opening ◽

Type Ia ◽

Dna Strand ◽

Dynamics Simulations ◽

Strand Passage

AbstractType IA topoisomerases cleave single-stranded DNA and relieve negative supercoils in discrete steps corresponding to the passage of the intact DNA strand through the cleaved strand. Although it is assumed type IA topoisomerases accomplish this strand passage via a protein-mediated DNA gate, opening of this gate has never been observed. We developed a single-molecule assay to directly measure gate opening of the E. coli type IA topoisomerases I and III. We found that following cleavage of single-stranded DNA, the protein gate opens by as much as 6.6 nm and can close against forces in excess of 16 pN. Key differences in the cleavage, ligation and gate dynamics of these two enzymes provide insights into their different cellular functions. The single-molecule results are broadly consistent with conformational changes obtained from molecular dynamics simulations. These results allow us to develop a mechanistic model of type IA topoisomerase-ssDNA interactions.

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The Implication of Inquiry Learning Approach Toward Students’ Achievement in Biology of X Grade Students of SMA Negeri 2 Surakarta

BIO-PEDAGOGI ◽

10.20961/bio-pedagogi.v2i1.5266 ◽

2012 ◽

Vol 2 (1) ◽

pp. 16

Author(s):

Astri Setiawati ◽

Alvi Rosyidi ◽

Bowo Sugiyarto

Keyword(s):

Inquiry Learning ◽

Affective Domain ◽

Cognitive Domain ◽

Learning Approach ◽

Free Inquiry ◽

Inquiry Approach ◽

Randomized Control ◽

Domain 3 ◽

Cluster Random Sampling ◽

Domain 4

This research aims are to find out: 1) the influence of inquiry approach toward student’s achievement in cognitive domain; 2) the influence of inquiry approach toward student’s achievement in affective domain; 3) the influence of inquiry approach toward student’s achievement in pshycomotor domain; 4) the best approach toward student’s achievement; and 5) the most effective approach toward student’s achievement of studying biology on X grade of SMA Negeri 2 Surakarta. This research is a quasi experiment research with randomized control only design using inquiry approach and conventional approach as independent variables and student’s achievement of study biology as the dependent variable. Here, it is acquired three classes as the samples, there are the student’s of class X-1 as the control class, student’s of class X-2 as the first experimental class, and student’s of class X-3 as the second experimental class. The population of this research is all of the student’s was X grade of SMA Negeri 2 Surakarta. The sample of this research was established by cluster random sampling. The techniques for collecting data are by using test, questionnaire, and documentation. The data were analyzed by anova and then by scheffe test. The conclusion of this research are: 1) There is an influence of inquiry approach toward student’s achievement in cognitive domain; 2) There is no influence of inquiry approach toward student’s achievement in affective domain; 3) There is an influence of inquiry approach toward student’s achievement in pshycomotor domain; 4) Modified free inquiry is the best approach to influence the student’s achievement based on cognitive domain, guided inquiry approach and modified free approach are better to influence the student’s achievement on pshycomotor domain; and 5) The most effective approach to influence the student’s achievement is modified free inquiry. Key Words: inquiry learning approach, achievement, Biology.

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Three-Dimensional Analysis of Component Stability of the Nellix Endovascular Aneurysm Sealing System After Treatment of Infrarenal Abdominal Aortic Aneurysms

Journal of Endovascular Therapy ◽

10.1177/1526602816678201 ◽

2016 ◽

Vol 24 (2) ◽

pp. 201-209 ◽

Cited By ~ 11

Author(s):

Bernhard Dorweiler ◽

Christian Boedecker ◽

Friedrich Dünschede ◽

Christian F. Vahl ◽

Marwan Youssef

Keyword(s):

Stent Graft ◽

Conformational Changes ◽

Three Dimensional ◽

Aortic Aneurysms ◽

3D Analysis ◽

Short Term ◽

Abdominal Aortic ◽

Overall Stability ◽

Type Ia ◽

Instructions For Use

Purpose: To assess short-term stability and conformational changes of the Nellix EndoVascular Aneurysm Sealing (EVAS) System using 3-dimensional (3D) analysis. Methods: Postoperative computed tomography (CT) scans obtained at 0, 3, and 12 months in 24 patients (mean age 75±7 years; 22 men) who underwent EVAS between December 2013 and December 2014 for intact abdominal aortic aneurysm (within the instructions for use) were evaluated for stent-graft deviation in multiple planes using dedicated 3D analysis software. In addition, 2D analysis using an anatomically fixed reference landmark was performed to assess craniocaudal migration. Clinical and follow-up data of the patients were recorded and matched with results of the imaging analysis. Results: Overall stability of the Nellix endografts was promising. Relevant conformational changes in the majority of cases were limited to the iliac graft segment and were clinically benign in all cases. Conversely, the only deviation of the proximal stent-graft segment was found in a patient with type Ia endoleak. Additional 2D analysis found relevant (≥5 mm) caudal migration of the Nellix stent-graft in 6 patients, including the one with the type Ia endoleak. In 3 patients, 3D analysis demonstrated the absence of relevant conformational changes of the endografts despite caudal migration. Conclusion: Overall stability of the separate EVAS stent-grafts is promising in the short term. Relevant conformational changes (stent-graft deviation) in the majority of cases were benign and confined to the iliac segment.

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Conformational Changes in Transmembrane Domain 4 of Presenilin 1 Are Associated with Altered Amyloid- 42 Production

Journal of Neuroscience ◽

10.1523/jneurosci.5090-14.2016 ◽

2016 ◽

Vol 36 (4) ◽

pp. 1362-1372 ◽

Cited By ~ 15

Author(s):

A. Tominaga ◽

T. Cai ◽

S. Takagi-Niidome ◽

T. Iwatsubo ◽

T. Tomita

Keyword(s):

Conformational Changes ◽

Transmembrane Domain ◽

Presenilin 1 ◽

Domain 4

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High resolution immunoelectron microscopic localization of functional domains of laminin, nidogen, and heparan sulfate proteoglycan in epithelial basement membrane of mouse cornea reveals different topological orientations.

The Journal of Cell Biology ◽

10.1083/jcb.107.4.1599 ◽

1988 ◽

Vol 107 (4) ◽

pp. 1599-1610 ◽

Cited By ~ 99

Author(s):

J C Schittny ◽

R Timpl ◽

J Engel

Keyword(s):

Basement Membrane ◽

Heparan Sulfate ◽

Heparan Sulfate Proteoglycan ◽

Low Density ◽

Sulfate Proteoglycan ◽

Lamina Densa ◽

Epithelial Basement Membrane ◽

Domain 3 ◽

Epithelial Basement ◽

Domain 4

Thin and ultrathin cryosections of mouse cornea were labeled with affinity-purified antibodies directed against either laminin, its central segments (domain 1), the end of its long arm (domain 3), the end of one of its short arms (domain 4), nidogen, or low density heparan sulfate proteoglycan. All basement membrane proteins are detected by indirect immunofluorescence exclusively in the epithelial basement membrane, in Descemet's membrane, and in small amorphous plaques located in the stroma. Immunoelectron microscopy using the protein A-gold technique demonstrated laminin domain 1 and nidogen in a narrow segment of the lamina densa at the junction to the lamina lucida within the epithelial basement membrane. Domain 3 shows three preferred locations at both the cellular and stromal boundaries of the epithelial basement membrane and in its center. Domain 4 is located predominantly in the lamina lucida and the adjacent half of the lamina densa. The low density heparan sulfate proteoglycan is found all across the basement membrane showing a similar uniform distribution as with antibodies against the whole laminin molecule. In Descemet's membrane an even distribution was found with all these antibodies. It is concluded that within the epithelial basement membrane the center of the laminin molecule is located near the lamina densa/lamina lucida junction and that its long arm favors three major orientations. One is close to the cell surface indicating binding to a cell receptor, while the other two are directed to internal matrix structures. The apparent codistribution of laminin domain 1 and nidogen agrees with biochemical evidence that nidogen binds to this domain.

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Perbedaan pekembangan kualitas hidup penderita Tb paru menggunakan instrumen indonesianwhoqol-breffquestionareterhadap fase pengobatan tuberculosis

Jurnal Kedokteran dan Kesehatan Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya ◽

10.32539/jkk.v5i3.6314 ◽

2018 ◽

Vol 5 (3) ◽

pp. 124-132

Author(s):

Pariyana Pariyana ◽

Iche Andriyani Liberty ◽

Bahrun Indawan Kasim ◽

Achmad Ridwan

Keyword(s):

Mycobacterium Tuberculosis ◽

T Test ◽

Domain 3 ◽

Domain 4

Tuberkulosis merupakan penyakit menular yang disebabkan oleh infeksi bakteri mycobacterium tuberculosis. Kualitas hidup pasien tuberkulosis merupakan hal penting untuk dinilai karena penurunan kualitas hidup penderita TB paru berhubungan dengan status kesehatan yang menyebabkan keterlambatan pengobatan dan berdampak negatif terhadap kelangsungan pengobatan sehingga menyebabkan pengobatan menjadi terputus atau tidak tuntas. Tujuan penelitian ini adalah untuk mengetahui perbedaan kualitas hidup penderita Tb paru terhadap fase pengobatan. Penelitian ini dilakukan pada 116 pasien Tb paru yang berobat ke Puskesmas Kota Palembanng. Analisis data menggunaka analisis deskriptif dan analisis inferensial (uji t test). Hasil penelitian didapatkan ada perbedaan kesehatan psikologis penderita Tb paru yang menjalani pengobatan fase awal dan fase lanjutan (p=0,036) dan tidak terdapat perbedaan kualitas hidup domain1 (kesejahteraan fisik) p=0,201, domain 3 (hubungan sosial) p=0,283, domain 4 (hubungan dengan lingkungan) p= 0,633 dan total domain(p=0,307) penderita Tb paru yang menjalani pengobatan fase awal dan fase lanjutan.Kesimpulan penelitian ini menunjukkan bahwa kualitas hidup penderita TB paru di Kota Palembang pada dimensi/domain kesejahteraan fisik, sosial dan lingkungankualitas hidup penderita Tb paru yang menjalani pengobatan fase awal dan fase lanjutan tidak berbeda bermakna, namun pada domain psikologis didapatkan ada perbedaan bermakna kualitas hidup penderita Tb paru yang menjalani pengobatan fase awal dan fase lanjutan, kualitas hidup Penderita Tb paru pada fase awal lebih buruk/kurang baik dibandingkan dengan kualitas hidup penderita Tb pada fase lanjutan.

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