scholarly journals Whole egg protein markedly increases blood vitamin D concentrations in male Sprague‐Dawley rats (1041.9)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Korey Van Wyk ◽  
Kevin Schalinske
Keyword(s):  
Vitamin D ◽  
Sprague Dawley ◽  
Egg Protein ◽  
Sprague Dawley Rats ◽  
Whole Egg

scholarly journals Effect of Nigella sativa against cisplatin induced nephrotoxicity in rats

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Amnah M.A. Alsuhaibani
Keyword(s):  
Vitamin D ◽  
Seed Treatment ◽  
Mineral Content ◽  
Renal Toxicity ◽  
Nigella Sativa ◽  
Bioactive Components ◽  
Sprague Dawley ◽  
Nigella Sativa Oil ◽  
Sprague Dawley Rats ◽  
Nigella Sativa Seed

In this study, the gross composition and mineral content of Nigella sativa seed powder (NSP) and fatty acid composition of Nigella sativa oil (NSO) were investigated. The ability of NSP, extract (NSE) and NSO in reducing the effects of cisplatin-induced renal toxicity in Sprague-Dawley rats were examined. The obtained results showed that NSP contains high amounts of carbohydrates, protein, and fiber while NSO has higher amounts of linoleicacid, oleic acid, and myristic acid. Rats treated with NSP, NSO, and NSE exhibitedreducedserum levels of urea, creatinine, and potassium, and a significant increase of Na, Na/K, vitamin D, nutritional markers, and antioxidant enzymes compared to the cisplatin-induced renal toxicity group receiving no Nigella sativa seed treatment. This study determined that all powder, oil, and extracts of N. sativa contain potent bioactive components that may aid in treatment against cisplatininduced renal toxicity in rats.

scholarly journals Ameliorative Potential of Riboceine, Vitamin D and Calcium on Amodaiquine‐Induced Reproductive Dysfunctions in Female Sprague‐Dawley Rats

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
SUNDAY OLUWADUROTIMI MOBOLAJI-JOBI ◽  
Ini-ibehe Essien OKOKO ◽  
Leke Jacob MEDUBI ◽  
Abayomi Olugbenga OKANLAWON
Keyword(s):  
Vitamin D ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
O. Merino ◽  
R. Sánchez ◽  
B. M. Gregorio ◽  
F. J. Sampaio ◽  
J. Risopatrón
Keyword(s):  
Vitamin D ◽  
Dna Fragmentation ◽  
High Fat Diet ◽  
Control Diet ◽  
Sperm Function ◽  
Sprague Dawley ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Sprague Dawley Rats ◽  
The Impact

Obesity has adverse effects on male fertility and usually is diagnosed with a prevalence of vitamin D deficiency (VD-). Discussion on the impact of obesity/VD- on sperm function has been limited. This study analyzed the effects of diet-induced obesity/VD- on viability and plasma membrane integrity (PMI), superoxide anion (O2-) level, and DNA fragmentation (DNAfrag) in sperm Sprague-Dawley rats. The males were randomized into four groups and fed for a period of 12 weeks: G1: control diet with vitamin D (C/VD+), G2: control diet without vitamin D (C/VD-), G3: high-fat diet with vitamin D (HF/VD+), and G4: high-fat diet without vitamin D (HF/VD-). Sperm function parameters were analyzed by flow cytometry. PMI percentages and O2- levels were not affected by any of the diets. DNA fragmentation was increasing significantly (p<0.05) in the spermatozoa of animals with diets vitamin D deficient (G2) and diet-induced obesity (G4). Our results allow us to point out that diet-induced obesity and VD- produce greater damage in DNA sperm of rats. The use of nutraceuticals containing vitamin D could be reducing the risk of fragmentation of DNA in spermatozoa.

scholarly journals Comparison of the Pharmacological Effects of Paricalcitol and Doxercalciferol on the Factors Involved in Mineral Homeostasis

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
J. Ruth Wu-Wong ◽  
Masaki Nakane ◽  
Gerard D. Gagne ◽  
Kristin A. Brooks ◽  
William T. Noonan
Keyword(s):  
Vitamin D ◽  
Cell Proliferation ◽  
Mrna Expression ◽  
Active Form ◽  
Parathyroid Cell ◽  
Pharmacological Effects ◽  
Sprague Dawley ◽  
Mineral Homeostasis ◽  
Sprague Dawley Rats ◽  
Serum Pth

Vitamin D receptor agonists (VDRAs) directly suppress parathyroid hormone (PTH) mRNA expression. Different VDRAs are known to have differential effects on serum calcium (Ca), which may also affect serum PTH levels since serum Ca regulates PTH secretion mediated by the Ca-sensing receptor (CaSR). In this study, we compared the effects of paricalcitol and doxercalciferol on regulating serum Ca and PTH, and also the expression of PTH, VDR, and CaSR mRNA. The 5/6 nephrectomized (NX) Sprague-Dawley rats on a normal or hyperphosphatemia-inducing diet were treated with vehicle, paricalcitol, or doxercalciferol for two weeks. Both drugs at the tested doses (0.042–0.33 g/kg) suppressed PTH mRNA expression and serum PTH effectively in the 5/6 NX rats, but paricalcitol was less potent in raising serum Ca than doxercalciferol. In pig parathyroid cells, paricalcitol and the active form of doxercalciferol induced VDR translocation from the cytoplasm into the nucleus, suppressed PTH mRNA expression and inhibited cell proliferation in a similar manner, although paricalcitol induced the expression of CaSR mRNA more effectively. The multiple effects of VDRAs on modulating serum Ca, parathyroid cell proliferation, and the expression of CaSR and PTH mRNA reflect the complex involvement of the vitamin D axis in regulating the mineral homeostasis system.

scholarly journals The Impact of Adult Vitamin D Deficiency on Behaviour and Brain Function in Male Sprague-Dawley Rats

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e71593 ◽  
Author(s):  
Jacqueline H. Byrne ◽  
Meggie Voogt ◽  
Karly M. Turner ◽  
Darryl W. Eyles ◽  
John J. McGrath ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Brain Function ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
The Impact

Therapeutic Effect of Vitamin D3 in a Rat Diffuse Axonal Injury Model

2005 ◽  
Vol 33 (1) ◽  
pp. 90-95 ◽  
Author(s):  
UA Malcok ◽  
G Sengul ◽  
HH Kadioglu ◽  
IH Aydin
Keyword(s):  
Vitamin D ◽  
Therapeutic Effect ◽  
Head Trauma ◽  
Axonal Injury ◽  
Diffuse Axonal Injury ◽  
Sprague Dawley ◽  
Post Injury ◽  
Sprague Dawley Rats ◽  
Injury Model ◽  
Impact Acceleration

We investigated the therapeutic effect of vitamin D3 in a rat diffuse axonal injury model. A total of 60 male Sprague-Dawley rats weighing 175 − 200 g were anaesthetized and subjected to head trauma using Marmarou's impact-acceleration model. The rats were then separated into two groups; one group was treated with vitamin D3 and the other with saline for up to 4 days after the head trauma. Rats from both groups were killed 1, 3 or 8 days post-injury. The brains were examined histopathologically and scored according to the level of neuronal, vascular and axonal damage. There were no significant differences between the groups after 1 or 3 days, but evaluation after 8 days revealed a significant improvement in the group treated with vitamin D3. Our data indicate that vitamin D3 has a beneficial effect in diffuse axonal injury and may be useful in the management of this condition.

Effects of 3,3′,4,4′,5-pentachlorobiphenyl (PCB126) on vertebral bone mineralization and on thyroxin and vitamin D levels in Sprague–Dawley rats

2009 ◽  
Vol 187 (2) ◽  
pp. 63-68 ◽  
Author(s):  
Pedro Alvarez-Lloret ◽  
P. Monica Lind ◽  
Ingrid Nyberg ◽  
Jan Örberg ◽  
Alejandro B. Rodríguez-Navarro
Keyword(s):  
Vitamin D ◽  
Bone Mineralization ◽  
Sprague Dawley ◽  
Vertebral Bone ◽  
Sprague Dawley Rats ◽  
Vitamin D Levels

scholarly journals Parental vitamin D deficiency during pregnancy is associated with increased blood pressure in offspring via Panx1 hypermethylation

2016 ◽  
Vol 311 (6) ◽  
pp. H1459-H1469 ◽  
Author(s):  
Laura M. G. Meems ◽  
Hasan Mahmud ◽  
Hendrik Buikema ◽  
Jörg Tost ◽  
Sven Michel ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Nutritional Deficiencies ◽  
Sprague Dawley ◽  
Pannexin 1 ◽  
Genome Wide ◽  
Sprague Dawley Rats ◽  
Large Vessels ◽  
Increased Susceptibility

Vitamin D deficiency is one of the most common nutritional deficiencies worldwide. Maternal vitamin D deficiency is associated with increased susceptibility to hypertension in offspring, but the reasons for this remain unknown. The aim of this study was to determine if parental vitamin D deficiency leads to altered DNA methylation in offspring that may relate to hypertension. Male and female Sprague-Dawley rats were fed a standard or vitamin D-depleted diet. After 10 wk, nonsibling rats were mated. The conceived pups received standard chow. We observed an increased systolic and diastolic blood pressure in the offspring from depleted parents (F1-depl). Genome-wide methylation analyses in offspring identified hypermethylation of the promoter region of the Pannexin-1 ( Panx1) gene in F1-depl rats. Panx1 encodes a hemichannel known to be involved in endothelial-dependent relaxation, and we demonstrated that in F1-depl rats the increase in blood pressure was associated with impaired endothelial relaxation of the large vessels, suggesting an underlying biological mechanism of increased blood pressure in children from parents with vitamin deficiency. Parental vitamin D deficiency is associated with epigenetic changes and increased blood pressure levels in offspring.

scholarly journals Vitamin D attenuates cerebral artery remodeling through VDR/AMPK/eNOS dimer phosphorylation pathway after subarachnoid hemorrhage in rats

2017 ◽  
Vol 39 (2) ◽  
pp. 272-284 ◽  
Author(s):  
Budbazar Enkhjargal ◽  
Jay Malaguit ◽  
Wing M Ho ◽  
Wu Jiang ◽  
Weifeng Wan ◽  
...  
Keyword(s):  
Vitamin D ◽  
Subarachnoid Hemorrhage ◽  
Cerebral Artery ◽  
Therapeutic Strategy ◽  
Cerebral Arteries ◽  
Sprague Dawley ◽  
Enos Expression ◽  
Sprague Dawley Rats ◽  
Artery Remodeling

The role of vitamin D3 (VitD3) in the upregulation of osteopontin (OPN) and eNOS in the endothelium of cerebral arteries after subarachnoid hemorrhage (SAH) is investigated. The endovascular perforation SAH model in Sprague-Dawley rats ( n = 103) was used. The VitD3 pretreatment (30 ng/kg) increased endogenous OPN and eNOS expression in cerebral arteries compared with naïve rats ( n = 5 per group). Neurobehavioral scores were significantly improved in Pre-SAH+VitD3 group compared with the SAH group. The effects of VitD3 were attenuated by intracerebroventricular (i.c.v) injections of siRNA for the vitamin D receptor (VDR) and OPN in Pre-SAH+VitD3+VDR siRNA and Pre-SAH+VitD3+OPN siRNA rats, respectively ( n = 5 per group). The significant increase of VDR, OPN and decrease of C44 splicing in the cerebral arteries of Pre-SAH+VitD3 rats lead to an increase in basilar artery lumen. The increase in VDR expression led to an upregulation and phosphorylation of AMPK and eNOS, especially dimer form, in endothelium of cerebral artery. The results provide that VitD3 pretreatment attenuates cerebral artery remodeling and vasospasm through the upregulation of OPN and phosphorylation of AMPK (p-AMPK) and eNOS (p-eNOS) at Ser1177-Dimer in the cerebral arteries. Vitamin D may be a useful new preventive and therapeutic strategy against cerebral artery remodeling in stroke patients.

Abstract P091: Vitamin D Supplementation Inhibits Blood Pressure and Uterine Artery Resistance Induced by Autoantibodies to the AT1 Receptor

Hypertension ◽  
2015 ◽  
Vol 66 (suppl_1) ◽  
Author(s):  
Jessica L Faulkner ◽  
Mark W Cunningham ◽  
Lorena M Amaral ◽  
Tarek Ibrahim ◽  
Denise C Cornelius ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Vitamin D ◽  
Uterine Artery ◽  
Resistance Index ◽  
Vitamin D Supplementation ◽  
At1 Receptor ◽  
Angiogenic Factor ◽  
Sprague Dawley ◽  
Pregnant Rats ◽  
Sprague Dawley Rats

Studies in our lab have previously shown that Vitamin D supplementation in the RUPP rat model of preeclampsia lowers blood pressure and reduces autoantibodies to the AT1 receptor (AT1-AA). Therefore, we sought to determine if the effects of Vitamin D supplementation to inhibit endothelial dysfunction and hypertension during pregnancy were mediated by AT1-AA reduction. We hypothesized that Vitamin D supplementation to AT1-AA-induced hypertensive pregnant rats would reduce anti-angiogenic factor soluble FMS-like tyrosine kinase-1 (sflt-1) and uterine artery resistance index (UARI) while improving blood pressure (MAP). Purified rat AT1-AA was infused (1:40) into Sprague-Dawley rats via miniosmotic pump from gestational day (GD) 12 to GD19. On GD14-18 we administered Vitamin D2 or D3 (VD2 or VD3) to AT1-AA rats (50ul/ml) by oral gavage. On GD18 indwelling carotid catheters were inserted and UARI assessed by Doppler sonography and MAP was measured on GD19. Consistent with previous studies, MAP was 119.0 mmHg in AT1-AA infused pregnant rats. MAP was unchanged with VD2 treatment at 121.7 mmHg (n=3), however, reduced to 109.3 mmHg (n=3) in AT1-AA+VD3 rats. Pup and placental weights were 1.79 and 0.46 g (n=3), respectively, in AT1-AA rats and were increased with VD2 treatment to 2.33 and 0.54 g (n=3) and to 2.39 and 0.56 g (n=3) in AT1-AA+VD3 rats. UARI was 0.577 (n=2) in AT1-AA rats but reduced with VD2 treatment to 0.491 (n=3) and VD3 to 0.452 (n=2). Plasma sflt-1, which is increased with AT1-AA infusion, was measured with ELISA and was >1050 pg/ml in AT1-AA rats (n=3) and greatly reduced in both AT1-AA+VD2 at 42.3 pg/ml (n=2) and AT1-AA+VD3 at 241.0 pg/ml (n=3). Our preliminary data demonstrate that Vitamin D supplementation improves uterine artery vascular resistance and sflt-1 and that these are potential mechanisms for improving fetal weight and hypertension that is induced by AT1-AA during pregnancy.

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