scholarly journals Effect of Nigella sativa against cisplatin induced nephrotoxicity in rats

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Amnah M.A. Alsuhaibani
Keyword(s):  
Vitamin D ◽  
Seed Treatment ◽  
Mineral Content ◽  
Renal Toxicity ◽  
Nigella Sativa ◽  
Bioactive Components ◽  
Sprague Dawley ◽  
Nigella Sativa Oil ◽  
Sprague Dawley Rats ◽  
Nigella Sativa Seed

In this study, the gross composition and mineral content of Nigella sativa seed powder (NSP) and fatty acid composition of Nigella sativa oil (NSO) were investigated. The ability of NSP, extract (NSE) and NSO in reducing the effects of cisplatin-induced renal toxicity in Sprague-Dawley rats were examined. The obtained results showed that NSP contains high amounts of carbohydrates, protein, and fiber while NSO has higher amounts of linoleicacid, oleic acid, and myristic acid. Rats treated with NSP, NSO, and NSE exhibitedreducedserum levels of urea, creatinine, and potassium, and a significant increase of Na, Na/K, vitamin D, nutritional markers, and antioxidant enzymes compared to the cisplatin-induced renal toxicity group receiving no Nigella sativa seed treatment. This study determined that all powder, oil, and extracts of N. sativa contain potent bioactive components that may aid in treatment against cisplatininduced renal toxicity in rats.

scholarly journals Nigella sativa oil extracts improves dyslipidaemia in high fat diet (HFD) animal model

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Roslina Abdul Rahim ◽  
Muhammad Taufiq Badaruddin ◽  
Qamar Uddin Ahmed ◽  
Siti Aesah @ Naznin Muhammad
Keyword(s):  
Animal Model ◽  
High Fat Diet ◽  
Nigella Sativa ◽  
Density Lipoprotein ◽  
Sprague Dawley ◽  
High Fat ◽  
Nigella Sativa Oil ◽  
Blood Biochemical ◽  
Sprague Dawley Rats ◽  
Oil Extract

Introduction: Nigella sativa has been reported to have anti-inflammatory and antioxidative properties. We aimed to investigate the effects of Nigella sativa oil extract on the high fat diet (HFD) fed animal model. Methods: Thirty male Sprague-Dawley rats were used. Four groups (n=7) were fed with high fat diet (HFD) rat pellet orally for 10 weeks. The rats with HFD groups were subsequently randomly divided again into 4 groups. One group was continued with HFD while the other 3 groups were continued with the HFD in addition to Nigella sativa oil extract treatment at three different concentrations (0.5, 1 and 1.5 ml/kg/day) for another 4 weeks. Blood biochemical analysis and histological assessment of liver were subsequently performed. Results: Animals fed with HFD had increased aspartate aminotransferase (AST), alkaline phosphatase (ALP), albumin, cholesterol/HDL ratio, triglyceride (TG) levels, but decreased globulin, albumin/globulin ratio and high density lipoprotein (HDL) levels. Meanwhile, HFD animals treated with Nigella sativa oil extract showed a significant increase in the globulin and HDL levels, but reduced ALP, albumin, cholesterol/HDL ratio and TG levels. Nigella sativa oil extract at the higher doses displayed much better effect. Histologically the liver in all groups exhibited micro vesicular steatosis. Conclusions: Nigella sativa oil extracts improve the dyslipidaemia in animal models with HFD.

scholarly journals Ameliorative Potential of Riboceine, Vitamin D and Calcium on Amodaiquine‐Induced Reproductive Dysfunctions in Female Sprague‐Dawley Rats

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
SUNDAY OLUWADUROTIMI MOBOLAJI-JOBI ◽  
Ini-ibehe Essien OKOKO ◽  
Leke Jacob MEDUBI ◽  
Abayomi Olugbenga OKANLAWON
Keyword(s):  
Vitamin D ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
O. Merino ◽  
R. Sánchez ◽  
B. M. Gregorio ◽  
F. J. Sampaio ◽  
J. Risopatrón
Keyword(s):  
Vitamin D ◽  
Dna Fragmentation ◽  
High Fat Diet ◽  
Control Diet ◽  
Sperm Function ◽  
Sprague Dawley ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Sprague Dawley Rats ◽  
The Impact

Obesity has adverse effects on male fertility and usually is diagnosed with a prevalence of vitamin D deficiency (VD-). Discussion on the impact of obesity/VD- on sperm function has been limited. This study analyzed the effects of diet-induced obesity/VD- on viability and plasma membrane integrity (PMI), superoxide anion (O2-) level, and DNA fragmentation (DNAfrag) in sperm Sprague-Dawley rats. The males were randomized into four groups and fed for a period of 12 weeks: G1: control diet with vitamin D (C/VD+), G2: control diet without vitamin D (C/VD-), G3: high-fat diet with vitamin D (HF/VD+), and G4: high-fat diet without vitamin D (HF/VD-). Sperm function parameters were analyzed by flow cytometry. PMI percentages and O2- levels were not affected by any of the diets. DNA fragmentation was increasing significantly (p<0.05) in the spermatozoa of animals with diets vitamin D deficient (G2) and diet-induced obesity (G4). Our results allow us to point out that diet-induced obesity and VD- produce greater damage in DNA sperm of rats. The use of nutraceuticals containing vitamin D could be reducing the risk of fragmentation of DNA in spermatozoa.

Effect of Poly(D, L-lactic acid) Nanoparticles as Triptolide Carrier on Abating Rats Renal Toxicity by NMR-Based Metabolic Analysis

2008 ◽  
Vol 8 (7) ◽  
pp. 3493-3499 ◽  
Author(s):  
Mingxing Liu ◽  
Jing Dong ◽  
Yajiang Yang ◽  
Xiangliang Yang ◽  
Huibi Xu
Keyword(s):  
Lactic Acid ◽  
Renal Toxicity ◽  
Circulatory System ◽  
Renal Lesion ◽  
Sprague Dawley ◽  
H Nmr ◽  
Metabolic Analysis ◽  
Sprague Dawley Rats ◽  
New Formulation

The aim of this study is to investigate the effect of poly(D, L-lactic acid) (PLA) nanoparticles as triptolide carrier on abating renal toxicity for Sprague Dawley rats after oral administration. Triptolide has severe toxicities on digestive, urogenital and blood circulatory system. High-resolution 600-MHz 1H-nuclear magnetic resonance (1H-NMR)-based metabolic analysis was performed on urine samples obtained from five groups of Sprague Dawley rats administrated with free triptolide and triptolide-loaded PLA nanoparticles at day 5, 10 and 15. The relative concentrations of biomarkers for renal lesion caused by triptolide were determined by 1H-NMR. The disorder of metabolism was characterized by the exceptional changes of the relative concentrations of succinate, 2-oxoglutarate and citrate. Similarly, the renal lesion was characterized by an increase of the relative concentrations of trimethylamine N-oxide and dimethylglycine, and a decrease of that of urea and allantoin. These results revealed that triptolide-loaded PLA nanoparticles might abate the renal toxicity of triptolide in comparison with identical doses of the free drug. The higher the dose (0.6 mg/kg), the more pronounced was this trend during long-term application. These results were further confirmed by histopathological changes. These results indicated that PLA nanoparticles provided a promising new formulation to abate the renal toxicity caused by triptolide.

scholarly journals Comparison of the Pharmacological Effects of Paricalcitol and Doxercalciferol on the Factors Involved in Mineral Homeostasis

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
J. Ruth Wu-Wong ◽  
Masaki Nakane ◽  
Gerard D. Gagne ◽  
Kristin A. Brooks ◽  
William T. Noonan
Keyword(s):  
Vitamin D ◽  
Cell Proliferation ◽  
Mrna Expression ◽  
Active Form ◽  
Parathyroid Cell ◽  
Pharmacological Effects ◽  
Sprague Dawley ◽  
Mineral Homeostasis ◽  
Sprague Dawley Rats ◽  
Serum Pth

Vitamin D receptor agonists (VDRAs) directly suppress parathyroid hormone (PTH) mRNA expression. Different VDRAs are known to have differential effects on serum calcium (Ca), which may also affect serum PTH levels since serum Ca regulates PTH secretion mediated by the Ca-sensing receptor (CaSR). In this study, we compared the effects of paricalcitol and doxercalciferol on regulating serum Ca and PTH, and also the expression of PTH, VDR, and CaSR mRNA. The 5/6 nephrectomized (NX) Sprague-Dawley rats on a normal or hyperphosphatemia-inducing diet were treated with vehicle, paricalcitol, or doxercalciferol for two weeks. Both drugs at the tested doses (0.042–0.33 g/kg) suppressed PTH mRNA expression and serum PTH effectively in the 5/6 NX rats, but paricalcitol was less potent in raising serum Ca than doxercalciferol. In pig parathyroid cells, paricalcitol and the active form of doxercalciferol induced VDR translocation from the cytoplasm into the nucleus, suppressed PTH mRNA expression and inhibited cell proliferation in a similar manner, although paricalcitol induced the expression of CaSR mRNA more effectively. The multiple effects of VDRAs on modulating serum Ca, parathyroid cell proliferation, and the expression of CaSR and PTH mRNA reflect the complex involvement of the vitamin D axis in regulating the mineral homeostasis system.

Nigella sativa Seed Protects Against Cadmium-induced Renal Toxicity in Rats

2020 ◽  
Vol 14 (2) ◽  
pp. 140-149
Author(s):  
Ikenna Kingsley Uchendu ◽  
Henshaw Uchechi Okoroiwu
Keyword(s):  
Low Dose ◽  
Renal Toxicity ◽  
Histopathological Examination ◽  
Nigella Sativa ◽  
Intraperitoneal Administration ◽  
Albino Rats ◽  
High Dose ◽  
Protective Effects ◽  
Oral Group ◽  
Nigella Sativa Seed

Background: Prevalence of chemical-induced renal injuries has been on a fast rise over the years and has become the leading cause of mortality and morbidity in the society, with environmental pollutants, heavy metals inclusive, seen as the causal agents. Recently, the role of medicinal foods in human health has gained considerable attention. Objective: We investigated the protective effects of methanolic extract of Nigella sativa (MENS) (Black seed) against cadmium-induced renal toxicity in albino rats. Methods: Twenty-five (25) male albino rats, weighing (150-170g), were randomly grouped into five groups: A-E. Group B (Negative Control) received intraperitoneal administration of cadmium chloride (CdCl2, 5mg/kg) only, group C received CdCl2 and low dose MENS (300mg/kg, oral), group D received CdCl2 and high dose MENS (600mg/kg, oral), group E (Positive control) received CdCl2 and Vitamin C (200mg/kg, oral), for 7 days. No treatment was administered to group A (Normal control). Renal injury was assessed by measuring serum levels of Na+, K+, creatinine and blood urea nitrogen (BUN) using standard methods. The kidneys were harvested for histopathological examination. Results: CdCl2 induced significant nephrotoxicity with marked elevation in the levels of biochemical markers of renal functions (p<0.05 or p<0.01); these were, however, ameliorated by a low dose of MENS. Histopathological examination of the kidney sections supported the biochemical findings. Conclusion: We conclude that Nigella sativa seed extract, at a low dose, is potentially nephroprotective against harmful chemical toxins such as cadmium.

scholarly journals The Impact of Adult Vitamin D Deficiency on Behaviour and Brain Function in Male Sprague-Dawley Rats

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e71593 ◽  
Author(s):  
Jacqueline H. Byrne ◽  
Meggie Voogt ◽  
Karly M. Turner ◽  
Darryl W. Eyles ◽  
John J. McGrath ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Brain Function ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
The Impact

Therapeutic Effect of Vitamin D3 in a Rat Diffuse Axonal Injury Model

2005 ◽  
Vol 33 (1) ◽  
pp. 90-95 ◽  
Author(s):  
UA Malcok ◽  
G Sengul ◽  
HH Kadioglu ◽  
IH Aydin
Keyword(s):  
Vitamin D ◽  
Therapeutic Effect ◽  
Head Trauma ◽  
Axonal Injury ◽  
Diffuse Axonal Injury ◽  
Sprague Dawley ◽  
Post Injury ◽  
Sprague Dawley Rats ◽  
Injury Model ◽  
Impact Acceleration

We investigated the therapeutic effect of vitamin D3 in a rat diffuse axonal injury model. A total of 60 male Sprague-Dawley rats weighing 175 − 200 g were anaesthetized and subjected to head trauma using Marmarou's impact-acceleration model. The rats were then separated into two groups; one group was treated with vitamin D3 and the other with saline for up to 4 days after the head trauma. Rats from both groups were killed 1, 3 or 8 days post-injury. The brains were examined histopathologically and scored according to the level of neuronal, vascular and axonal damage. There were no significant differences between the groups after 1 or 3 days, but evaluation after 8 days revealed a significant improvement in the group treated with vitamin D3. Our data indicate that vitamin D3 has a beneficial effect in diffuse axonal injury and may be useful in the management of this condition.

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