SOME STUDIES OF WOUND HEALING: EXPERIMENTAL METHODS, EFFECT OF ASCORBIC ACID AND EFFECT OF DEUTERIUM OXIDE

AbstrakJeruk Nipis (Citrus aurantifolia S.) merupakan salah satu tanaman obat keluarga yang banyak terdapat ditengah masyarkat dan banyak digunakan sebagai ramuan tradisional. Bagian yang sering digunakan adalah air perasannya, dengan salah satu manfaat dapat digunakan untuk menghilangkan jerawat serta penyembuhan luka agar tidak terjadi abses. Jerawat dan abses pada luka merupakan salah satu infeksi yang disebabkan oleh bakteri Staphylococcus aureus.Tujuan Penelitian ini adalah untuk mengetahui daya hambat air perasan buah jeruk nipis (Citrus aurantifolia S.) terhadap pertumbuhan bakteri Staphylococcus aureus secara invitro. Penelitian dilakukan dengan metoda eksperimental laboratorium dengan desain postest only control group design yang dilakukan di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas.Hasil penelitian menunjukan bahwa air perasan buah jeruk nipis memiliki daya hambat terhadap pertumbuhan bakteri Staphylococcus aureus dengan berbagai konsentrasi yaitu 25%, 50%, 75%, dan 100% dan terdapat pengaruh lama kontak terhadap pertumbuhan bakteri dimana bakteri tidak tumbuh seteleh kontak 5 menit pertama dan diikuti menit-menit berikutnya dengan air perasan buah jeruk nipis konsentrasi 100%. Jadi, semakin tinggi konsentrasi air perasan buah jeruk nipis dan semakin lama kontak dengan bakteri Staphylococcus aureus maka daya hambatnya semakin baik.Kata kunci: Uji Daya Hambat, Air Perasan Buah Jeruk Nipis, Staphylococcus aureus.Abstract Lime (Citrus aurantifolia S.) is kind of family’s herbal medicine, most using in the community is widely used as a traditional herb. The most common used part is the lime fruit squeeze with one of the function is used for removing acne and wound healing to prevent the form of abscess. Pimples and abscesses of the wound is one of the infections caused by the bacterium Staphylococcus aureus.The purpose of this study was to determine the inhibition of lime fruit (Citrus aurantifolia S.) squeeze towards the growth of the bacteria Staphylococcus aureus in vitro condition. The study was conducted with laboratory experimental methods to the design of control group design postest only performed at the Laboratory of Microbiology Faculty of Medicine, University of Andalas.The results showed that the lime fruit (Citrus aurantifolia S.) squeeze has the ability to inhibite the bacterial growth of Staphylococcus aureus with various concentrations of 25%, 50%, 75%, and 100% and there is the effect of contact time on the growth of bacteria which the bacteria do not grow after contact the first 5 minutes and the next minute followed by lime fruit squeeze with 100% concentration lime fruit squeeze. Thus, the higher the concentration of lime fruit squeeze and the longer the contact with the bacteria Staphylococcus aureus is the better towards.Keywords:Inhibition test, The Lime Fruit Squeeze, Staphylococcus Aureus.

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Ascorbic acid deficiency impairs wound healing in surgical patients: Four case reports

International Journal of Surgery Open ◽

10.1016/j.ijso.2016.02.009 ◽

2016 ◽

Vol 2 ◽

pp. 15-18 ◽

Cited By ~ 9

Author(s):

A. Bikker ◽

J. Wielders ◽

R. van Loo ◽

M. Loubert

Keyword(s):

Wound Healing ◽

Ascorbic Acid ◽

Case Reports ◽

Surgical Patients ◽

Ascorbic Acid Deficiency ◽

Acid Deficiency

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Ascorbic acid in the normal and regenerating tail of the house lizard, Hemidactylus flaviviridis

Development ◽

10.1242/dev.26.2.285 ◽

1971 ◽

Vol 26 (2) ◽

pp. 285-293

Author(s):

R. V. Shah ◽

P. K. Hiradhar ◽

D. K. Magon

Keyword(s):

Wound Healing ◽

Ascorbic Acid ◽

Matrix Material ◽

Growth Period ◽

Tail Regeneration ◽

The Matrix ◽

Metabolic Activities ◽

Vitamin Level ◽

Hemidactylus Flaviviridis

The concentration of ascorbic acid (AA) and the histochemical distribution of the vitamin in the normal and regenerating tail of the gekkonid lizard, Hemidactylus flaviviridis, have been investigated. In the regenerating tail of the lizard the AA concentration almost doubles during wound healing and becomes fivefold during differentiation. However, it falls almost to the normal level during the blastema phase (i.e. period between wound healing and differentiation). Again, during the growth period (i.e. after differentiation) the AA concentration gradually becomes reduced, reaching the normal mark as the regenerate regains the full length of the original tail. Nevertheless, the vitamin level does not fall below the normal mark at any stage of regeneration. Increase of ascorbic acid during wound healing is thought to be mainly due to increased demand for the vitamin at the broken ends of the stump tissues, for their repair and formation of wound epithelium; the vitamin is known to help these processes. A fivefold increase of the vitamin during the differentiation period corresponds to an increased pace of laying down of the matrix material for the connective tissues, suggesting the role of ascorbic acid in the formation of collagen and mucopolysaccharides. Besides, the role of ascorbic acid in lipid and carbohydrate metabolism is also important during tail regeneration. Fluctuations in the vitamin level during different phases of tail regeneration are correlated with various states of metabolic activities of the corresponding phases.

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In Vitro Effect of Ascorbic Acid on the Proliferation of Bovine Scleral and Tenon's Capsule Fibroblasts

European Journal of Ophthalmology ◽

10.1177/112067219800800109 ◽

1998 ◽

Vol 8 (1) ◽

pp. 37-41 ◽

Cited By ~ 12

Author(s):

P.O. Denk ◽

M. Knorr

Keyword(s):

Wound Healing ◽

Ascorbic Acid ◽

Healing Process ◽

Wound Healing Process ◽

Filtration Surgery ◽

Glaucoma Filtration Surgery ◽

Tenon’S Capsule ◽

Tenon's Capsule ◽

Vitro Effect

The success of glaucoma filtration surgery depends mainly on an incomplete wound healing process in the area of the fistula. Since Kornblueth and Tenenbaum's investigations in 1956 it has been known that aqueous humour has intrinsic antiproliferative properties. It is assumed that ascorbic acid is involved in the regulation of the wound healing process after filtration surgery. To evaluate the antiproliferative effect of ascorbic acid in vitro, we used cultured fibroblasts of bovine Tenon's capsule and bovine sclera. Incubation of these cells with ascorbic acid at concentrations ranging from 0.5 to 3 mM/L led to dose-dependent inhibition of cell proliferation. The half-maximal inhibitory concentration was 1.0 mM/L for both cell types. Physiological concentrations of ascorbic acid may be valuable in the pharmacological prevention of failure of glaucoma filtration surgery. However, extensive clinical investigations are needed to clarify whether topical intraoperative or postoperative as well as oral administration of ascorbic acid inhibits fibroblast proliferation after glaucoma filtration surgery.

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EFFECT OF MANIHOT ESCULENTA AQUEOUS EXTRACT AND THERAPEUTIC ULTRASOUND IN ACCELERATING THE WOUND HEALING PROCESS IN VITRO

Jurnal Teknologi ◽

10.11113/jt.v81.13135 ◽

2019 ◽

Vol 81 (4) ◽

Author(s):

Ulfah Anwar ◽

Siti Pauliena Mohd Bohari

Keyword(s):

Wound Healing ◽

Ascorbic Acid ◽

Aqueous Extract ◽

Manihot Esculenta ◽

Wound Closure ◽

Healing Process ◽

Therapeutic Ultrasound ◽

Wound Healing Process ◽

Time Interval

The aim of this research is to investigate the wound healing process in in vitro by combining the Manihot esculenta aqueous extract and therapeutic ultrasound. Firstly, the optimization seeding densities of HSF cell 1184 in six-well plate, and then followed by the scratch assay experiment. The scratched that made was treated with the remedial treatments (Manihot esculenta aqueous extract only; ascorbic acid+ therapeutic ultrasound; Manihot esculenta aqueous extract+ ascorbic acid; Manihot esculenta aqueous extract+ therapeutic ultrasound and also the combination of these three materials). The rate of wound closure was observed and analysed at a time interval of 0, 2, 4, 6, 8, 10 and 24 h by using image J software. Then, the cells viability were analysed using the MTT assay. The result showed that Manihot esculenta aqueous extract coupled with specific dose therapeutic ultrasound represents a significantly high rate of wound closure at 96.10 % with the cell numbers at 5.44×105 cells/mL when compared to the other combination therapy. The finding of this study revealed that Manihot esculenta aqueous extract 200 µg/mL and the therapeutic ultrasound specific dose (3 MHz, 300 mWatt/cm2, 50% in 5 min) have the potential in accelerating wound healing process of cells in in vitro.

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