Can C-Reactive Protein Function as a Surrogate Marker of Preeclampsia?

2002 ◽  
Vol 99 (Supplement) ◽  
pp. 14S
Author(s):  
Deena S. Tajran ◽  
Harrihar A. Pershadsingh
Keyword(s):  
Protein Function ◽  
Surrogate Marker ◽  
C Reactive Protein ◽  
Reactive Protein

scholarly journals 1716. Baseline Serum C-Reactive Protein Level Predicts Mortality in Cryptococcal Meningitis

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S629-S629
Author(s):  
Supavit Chesdachai ◽  
Nicole Engen ◽  
Joshua Rhein ◽  
Lillian Tugume ◽  
Tadeo kiiza. Kandole ◽  
...  
Keyword(s):  
Cryptococcal Meningitis ◽  
Surrogate Marker ◽  
First Episode ◽  
Event Analysis ◽  
C Reactive Protein ◽  
Serum Samples ◽  
Hiv Viral Load ◽  
Baseline Serum ◽  
Reactive Protein ◽  
Serum Crp

Abstract Background C-reactive protein (CRP) is an acute-phase protein produced by the liver in response to systemic inflammation. CRP is a helpful surrogate biomarker widely used in various infections, particularly for following the progression and resolution of infection. We aimed to determine the association between baseline CRP level and cryptococcal meningitis outcome. Methods We reviewed 168 prospectively enrolled HIV-infected Ugandans with confirmed first-episode cryptococcal meningitis. Baseline serum samples collected within 5 days from diagnosis had CRP levels measured and categorized into quartiles. We compared baseline serum CRP with 18-week survival using unadjusted time-to-event analysis. Results Of 168 participants, the first quartile of baseline serum CRP was 83.6 mg/L. Baseline CD4 count, HIV viral load, and cerebrospinal fluid results did not differ by quartile. Participants with CRP > 49.5 mg/L more likely presented with Glasgow Coma Scale <15 (P = 0.03). The 18-week mortality rate was 54.8% (46/84) in the highest two quartile CRP groups (49.5 mg/L), 40.5% (17/42) in the mid-range CRP group (29–49.5 mg/L), and 14.3% (6/42) in the low CRP group (<29 mg/L) (P < 0.001) (Figure 1). Conclusion Higher baseline serum CRP is associated with increased mortality in HIV-infected individuals with first-episode cryptococcal meningitis. The serum CRP could be a surrogate marker for undiagnosed co-infections or may reflect immune dysregulation leading to worse outcomes in persons with advanced AIDS and concomitant cryptococcal meningitis. Additional studies investigating more specific inflammatory biomarkers and the longitudinal trend in CRP with effective therapy would be informative. Disclosures All authors: No reported disclosures.

scholarly journals Baseline Serum C-Reactive Protein Level Predicts Mortality in Cryptococcal Meningitis

2020 ◽  
Vol 7 (12) ◽  
Author(s):  
Supavit Chesdachai ◽  
Nicole W Engen ◽  
Joshua Rhein ◽  
Lillian Tugume ◽  
Tadeo Kiiza Kandole ◽  
...  
Keyword(s):  
Cryptococcal Meningitis ◽  
Surrogate Marker ◽  
First Episode ◽  
Event Analysis ◽  
C Reactive Protein ◽  
Hiv Viral Load ◽  
Baseline Serum ◽  
Reactive Protein ◽  
Serum Crp ◽  
Fourth Quartile

Abstract Background C-reactive protein (CRP) is an acute phase protein produced by the liver in response to systemic inflammation. CRP is a helpful surrogate biomarker used for following the progression and resolution of infection. We aimed to determine the association of baseline CRP level and the temporal change in CRP over time with cryptococcal meningitis outcome. Methods We reviewed 168 prospectively enrolled HIV-infected Ugandans with confirmed first-episode cryptococcal meningitis. Baseline plasma CRP collected within 5 days of meningitis diagnosis was categorized into quartiles. We compared baseline CRP with 18-week survival using time-to-event analysis. Results Of 168 participants, the baseline first quartile of serum CRP was &lt;29.0 mg/L, second quartile 29.0–49.5 mg/L, third quartile 49.6–83.6 mg/L, and fourth quartile &gt;83.6 mg/L. Baseline CD4 count, HIV viral load, and cerebrospinal fluid results did not differ by CRP quartile. Participants with CRP &gt;49.5 mg/L more likely presented with Glasgow Coma Scale (GCS) &lt;15 (P = .03). The 18-week mortality rate was 55% (46/84) in the highest 2 quartile CRP groups (&gt;49.5 mg/L), 41% (17/42) in the mid-range CRP group (29.0–49.5 mg/L), and 14% (6/42) in the low-CRP group (&lt;29.0 mg/L; P &lt; .001). After adjustment for possible confounding factors including GCS &lt;15, CRP remained significantly associated with mortality (adjusted hazard ratio, 1.084 per 10 mg/L; 95% CI, 1.031–1.139; P = .0016). Conclusions Higher baseline CRP is associated with increased mortality in HIV-infected individuals with first-episode cryptococcal meningitis. CRP could be a surrogate marker for undiagnosed coinfections or may reflect immune dysregulation, leading to worse outcomes in persons with advanced AIDS and concomitant cryptococcal meningitis.

scholarly journals Absolute Lymphocytes, Ferritin, C-Reactive Protein, and Lactate Dehydrogenase Predict Early Invasive Ventilation in Patients With COVID-19

2020 ◽  
Author(s):  
Salvador Payán-Pernía ◽  
Lucía Gómez Pérez ◽  
Ángel F Remacha Sevilla ◽  
Jordi Sierra Gil ◽  
Silvana Novelli Canales
Keyword(s):  
Lactate Dehydrogenase ◽  
Classification Tree ◽  
Surrogate Marker ◽  
Area Under The Curve ◽  
Absolute Lymphocyte Count ◽  
Limited Resources ◽  
Invasive Ventilation ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
A Value

Abstract Objective Early detection of patients with COVID-19 who will need mechanical invasive ventilation (MIV) may aid in delivering proper care and optimizing the use of limited resources. Methods In this single-center retrospective observational study, we aimed to identify simple laboratory parameters that in combination with ferritin (a surrogate marker of severe inflammation) may help predict early (first 48 hours) MIV. A total of 160 patients with COVID-19 in whom serum ferritin, absolute lymphocyte count (ALC), platelet count, C-reactive protein (CRP), and lactate dehydrogenase (LDH) had been analyzed at admission were included. Results We found that ferritin, LDH, ALC, and CRP predicted with 88% accuracy the probability of early MIV. Results indicated that LDH showed the greater area under the curve (AUC), with a value of 89.1%. Using the AUC, we established cutoff values for clinical application. Finally, we developed a classification tree based on LDH for its clinical use. Conclusion Ferritin, LDH, ALC, and CRP predict with 88% accuracy the probability of early MIV.

scholarly journals To study the correlation of high sensitivity C reactive protein levels with asthma control in children

2016 ◽  
Vol 4 (1) ◽  
pp. 145
Author(s):  
Nithiyanantham Ramakrishnan ◽  
Shobhana Sivathanu ◽  
Sowmya Sampath ◽  
Suresh David JH ◽  
Thuthi Mohan
Keyword(s):  
Asthma Control ◽  
Inhaled Corticosteroids ◽  
Surrogate Marker ◽  
Persistent Asthma ◽  
Inverse Correlation ◽  
High Sensitivity ◽  
Mean Value ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Significant Difference

Background: Childhood asthma appears to be increasing in prevalence despite advancements in the care of asthma. In asthma, local as well as systemic inflammation contributes to the pathogenesis. Thus successful management depends on controlling this inflammation by appropriate doses of inhaled corticosteroids. High sensitivity C reactive protein (hsCRP) is an easily measurable marker of inflammation and its level can be used as a diagnostic tool in assessing control of asthma. The objective of this study was study the correlation of serum hsCRP levels with asthma control in childrenMethods: It is an observational study conducted in the Pediatric asthma clinic of a Government Medical College in south India. The study population consisted of 75 asthmatic children aged 5-15 years. Children with persistent asthma were classified into three groups based on GINA guidelines. Serum hsCRP levels were measured in all the three groups.Results: Among the 75 children 33% belonged to controlled group, 35% to uncontrolled group and 33% to partly controlled group. There was a significant difference in hsCRP levels between controlled and uncontrolled groups. The mean value of hsCRP in controlled group was 0.93±1.3 mg/L whereas in the uncontrolled group it was 2.73±2.46 mg/L. Higher levels of hsCRP were found in the uncontrolled asthma group.Conclusions: There is an inverse correlation between hsCRP levels and asthma control in children. High hsCRP levels have a potential to be used as a surrogate marker for poor control of asthma and can thus be used as a guide for adjustment of dosage of inhaled corticosteroids. 

Purification of Recombinant Mouse C-Reactive Protein from Pichia Pastoris GS115 by Nickel Chelating Sepharose Fast-Flow Affinity Chromatography and P-Aminophenyl Phosphoryl Choline Agarose Resin Affinity Chromatography in Tandem

2021 ◽  
Author(s):  
Bin Cheng ◽  
Di Wu ◽  
Ke Wu ◽  
Xiao-Ping Huang ◽  
Jian-Min Lv ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Affinity Chromatography ◽  
Protein Function ◽  
Recombinant Expression ◽  
Expression System ◽  
Post Translational Modification ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Phosphoryl Choline ◽  
Fast Flow

Abstract C-reactive protein (CRP) is a circulating marker of inflammation yet with ill-defined biological functions. This is partly due to the uncharacterized activities of endogenous CRP in mice, the major animal model used to define protein function. The hurdles for purification and characterization of mouse CRP are its low circulating levels and the lack of specific antibodies. To clear these hurdles, here we developed an efficient expression system by constructing recombinant Pichia pastoris cells for secretion of native conformation mouse CRP. The recombinant expression of mouse CRP in Escherichia coli failed to yield sufficient amount of native protein, reflecting the importance of post-translational modification of glycosylation in aiding proper folding. By contrast, sufficient amount of native mouse CRP was successfully purified from P. pastoris. Preliminary purification was performed by Nickel Chelating Sepharose Fast-Flow affinity chromatography with 6 × His tags attached to the protein. Subsequently, p-Aminophenyl Phosphoryl Choline Agarose resin affinity chromatography was used for tandem purification. The purified mouse CRP showed native pentamer and capabilities of PC binding. Moreover, the 6 × His tag provides a convenient tool for detecting the interactions of mouse CRP with ligands.

Highly sensitive C reactive protein (hsCRP) as a surrogate marker of head and neck cancer.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e17034-e17034
Author(s):  
Preethi Bangalore Lakshmangowda ◽  
Adarsh K ◽  
T.R Arul Ponni
Keyword(s):  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer ◽  
Inflammatory Responses ◽  
Surrogate Marker ◽  
Cancer Case ◽  
Control Group ◽  
Case Group ◽  
C Reactive Protein ◽  
Reactive Protein

e17034 Background: High-sensitivity C-reactive protein (hsCRP) an acute phase inflamatory reactant protein, has been used to predict the risk of cardiovascular disease healthy individuals and to monitor treatment responses. Epidemiological evidence also points to link between inflammation and development of cancer, i.e. long-term inflammation and dysplasia. Worldwide ~ 15 % of the cancer incidence is associated with microbial infection. NSAIDs have been used for cancer preventionin familial adenomatous polyposis. The objective of study is to evaluate the association between hs-CRP and head and neck cancer (SCCHN). Methods: Prospective, cross sectional case-control study was under taken involving 36 SCCHN (cases) and 36 normal volunteers age matched (controls). Cases staged as per UICC TNM. The study subjects 4ml clotted blood, centrifuged, serum stored at -20°C. hsCRP (mg/L) determined using Quantitative Immunoturbidimetric method. Statistical analysis was performed using SPSS. Results: Evaluable subjects = 72, Cases (Cancer patients) = 36 & Control = 36. Age group range 18 to 80 yrs. Mean Age Cases 59.5+9.9 v/s Control 59+96. M: F Cases- 25:9 v/s Control 27:9. In Cases Oral Ca – 22(61.1%) & Oropharynx - 14 (38.9%).T4 – 20(55.6%), T3-8(22.2%) & T2-8(22.2%). N3-3(8.3%), N2-14(38.9%), N1-5(13.9%) & N0-14(38.9%). The hsCRP of Control group v/s Cancer Case group = 3.03 ± 2.61 v/s19.23 ± 19.003 respectively (P < 0.0001). hsCRP for Oral Cancer – 15.07+7.66 & OPX – 25.77+28.31. In the case group, the mean hsCRP levels with tumor size T2 was 119.36 ± 7.0, T3 was 21.32 ± 23.27 , T4 was 18.3350 ± 21.0915 and with Node size N0- 20.3 ± 35.07, N1- 13.8 ± 5.37, N2 -21.6 ± 25.022, N3 - 122.1 ± 1.7. Conclusions: Inflammatory responses play decisive roles at different stages of tumor development, initiation, promotion, malignant conversion, invasion, and metastasis. This study shows serum hsCRP levels were significantly elevated in SCCHN cases compared to age matched normal control subjects. hsCRP Can be used as a surrogate marker of SCCHN. Measuring and charting hsCRP values can prove useful in determining disease progress or the effectiveness of treatments, Furthers studies are contemplated.

Prognostic role of serum concentrations of high-sensitivity C-reactive protein in patients with metastatic colon rectal cancer: Results from the ITACa trial.

2015 ◽  
Vol 33 (3_suppl) ◽  
pp. 617-617 ◽  
Author(s):  
Andrea Casadei Gardini ◽  
Silvia Carloni ◽  
Emanuela Scarpi ◽  
Paolo Maltoni ◽  
Romolo Dorizzi ◽  
...  
Keyword(s):  
Surrogate Marker ◽  
High Sensitivity ◽  
Serum Levels ◽  
Phase Iii ◽  
C Reactive Protein ◽  
Prognostic Role ◽  
Carcinoma Of The Colon ◽  
Reactive Protein ◽  
Hs Crp

617 Background: Serum levels of interleukine-6 and C-reactive protein are significatively higher in patients with neoplastic conditions. Therefore, the determination of high-sensitivity C-reactive protein (hs-PCR) has been widely used as a surrogate marker for chronic elevation of circulating cytokines. Increased hs-CRP concentrations have been reported in many conditions, in particular in patients with cardiovascular diseases, obesity, diabetes, autoimmunity, inflammatory bowel diseases and cancer risk. Some authors, on the basis of these findings, have encouraged further studies to clarify the etiologic and prognostic role of the aforementioned test. Our study has been conducted in patients enrolled in the phase III prospective multicentric randomized “Italian Trial in Advanced Colorectal Cancer (ITACa),” in order to assess hs-CRP levels at diagnosis and their significance with respect to overall survival (OS) and progresion free survival. Methods: Peripheral blood samples from 133 consecutive patients were collected into EDTA tubes. The collection was obtained before the beginning of first line chemotherapy. The supernatant was immediately transfer into a cryovial and stored at –80°C. Samples were thawed and hs-CRP has been measured with Cobas c501 analyzer. Results: Levels of hs-CRP >13.1 mg/L were associated with a worse median PFS, 8.9 months (95% CI 6.8-9.6) vs. 12.1 months (95% CI 9.3-14.9) in patients with levels <13.1 mg/L (p<0.0001). Similarly, levels >13.1 mg/L were associated with worse median OS, 14.4 (95% CI 11.5-17.1) vs. 28.8 (95% CI 24.3-36.6) in patients with a concentration <13.1 mg/L (p<0.0001). In multivariate analysis, hs-PCR adjusted for baseline factors including age (<70, ≥70 years), gender, ECOG perfomance status (0,1-2), tumor localization (rectum, colon), stage at diagnosis (I-III, IV), CT regimen (Folfiri, Folfox), KRAS status (wild type, mutant), site of metastases (liver, other metastases), was found to be independently associated with PFS and OS. Conclusions: Our study demonstrates the prognostic value of hs-CRP in patients with metastatic carcinoma of the colon and rectum.

scholarly journals Vitamin A and systemic inflammation as protective factors in multiple sclerosis

2013 ◽  
Vol 19 (8) ◽  
pp. 1046-1051 ◽  
Author(s):  
Jonatan Salzer ◽  
Göran Hallmans ◽  
Maria Nyström ◽  
Hans Stenlund ◽  
Göran Wadell ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Vitamin A ◽  
Surrogate Marker ◽  
High Sensitivity ◽  
Retinol Binding Protein ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Young Subjects ◽  
Hs Crp

Background: Vitamin A is important for the immune system, and might suppress inflammatory activity in multiple sclerosis (MS). Objectives: We aimed to examine if vitamin A levels were associated with MS risk in samples collected prospectively and during gestation. Methods: We measured Retinol Binding Protein (RBP – a surrogate marker for vitamin A) and high-sensitivity C-reactive protein (hs-CRP) levels, in (1) prospectively collected biobank blood samples from MS cases and controls, and (2) gestational samples where the offspring had later developed MS, and gestational control samples. The risk of MS was calculated using matched multivariable logistic regression adjusted for confounders. Results: In prospective samples, RBP levels within the second quintile (vs. the first) were associated with a lower MS risk (OR = 0.38, 95% CI 0.19–0.74). No effect on MS risk in the offspring by gestational RBP levels was found. In young subjects hs-CRP levels ≥10 mg/l in prospective samples were associated with a lower MS risk (OR = 0.36, 95% CI 0.14–0.95). Conclusions: Our results suggest that sub-optimal vitamin A levels may be associated with MS risk. The association between hs-CRP levels and MS risk in young subjects may support the role of the hygiene hypothesis in MS aetiology.

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