The Administration of Recombinant Thyrotrophic Hormone (rTSH) to Culture Medium Improves Islet Cell Viability in Rats.

2014 ◽  
Vol 98 ◽  
pp. 690
Author(s):  
Colak N. Bozkurt ◽  
F. Pinarli ◽  
T. Ulus ◽  
S. Mercan ◽  
E. Cakal ◽  
...  
Keyword(s):  
Cell Viability ◽  
Islet Cell ◽  
Culture Medium ◽  
Thyrotrophic Hormone

Indirect Cytotoxicity Evaluation of Silver Doped Bioglass Ag-S70C30 on Human Primary Keratinocytes

2005 ◽  
Vol 284-286 ◽  
pp. 431-434 ◽  
Author(s):  
U. Lohbauer ◽  
G. Jell ◽  
Priya Saravanapavan ◽  
Julian R. Jones ◽  
Larry L. Hench
Keyword(s):  
Cell Viability ◽  
Glass Powder ◽  
Culture Medium ◽  
Neutral Red ◽  
Epidermal Keratinocytes ◽  
Mitochondrial Activity ◽  
Human Epidermal Keratinocytes ◽  
Negative Effect ◽  
Healing Agent ◽  
Gel Glasses

Bioactive gel-glasses, such as the silver-doped Ag-S70C30 glass, can be used to modify the inflammatory response in a local body compartment such as in acne lesions and in nonhealing dermal wounds. In this study, the cytotoxicity of soluble silver, calcium and silica ions on human epidermal keratinocytes was investigated by measurements of mitochondrial activity (MTT assay) and neutral red dye uptake (NR assay). Ag-S70C30 extracts were prepared by soaking glass powder in complete culture medium at concentrations of 1 mg/ml and 2 mg/ml (mg of glass powder per ml of culture medium). Silver concentrations for both concentrations of approximately 1 ppm were detected by inductive coupled plasma analysis (ICP). No negative effect on the cell viability was measured for an initial gel-glass concentration of 1 mg/ml and for the two shortest extraction times at a concentration of 2 mg/ml. Based on the results from MTT/ NR assays, a pH rise of approximately one unit had no negative effect on the NHEK-A cell viability. This preliminary study on keratinocyte viability merits future investigations on silver bioglass as a novel antimicrobial wound healing agent.

scholarly journals The effects of 50 Hz magnetic field–exposed cell culture medium on cellular functions in FL cells

2019 ◽  
Vol 60 (4) ◽  
pp. 424-431 ◽  
Author(s):  
Yue Fei ◽  
Liling Su ◽  
Haifeng Lou ◽  
Chuning Zhao ◽  
Yiqin Wang ◽  
...  
Keyword(s):  
Cell Viability ◽  
Signaling Pathways ◽  
Relative Permittivity ◽  
Culture Medium ◽  
Biological Effects ◽  
Mapk Signaling ◽  
Mitogen Activated Protein Kinase ◽  
Cell Counting ◽  
International Agency ◽  
Dependent Manner

Abstract Although extremely low frequency magnetic fields (ELF-MFs) have been classified as a possible carcinogen for humans by the International Agency for Research on Cancer (IARC), their biological effects and underlying mechanisms are still unclear. Our previous study indicated that ELF-MF exposure influenced the relative permittivity of the saline solution, suggesting that the MF exposure altered physical properties of the solution. To explore the biophysical mechanism of ELF-MF–induced biological effects, this study examined the effects of 50 Hz sinusoidal MF at 0–4.0 mT on the permittivity of culture medium with phase-interrogation surface plasmon resonance (SPR) sensing. Then, the biological effects of MF pre-exposed culture medium on cell viability, the mitogen-activated protein kinase (MAPK) signaling pathways, oxidative stress, and genetic stabilities were analyzed using Cell Counting Kit-8, western blot, flow cytometry, γH2AX foci formation, and comet assay. The results showed that SPR signals were decreased under MF exposure in a time- and dose-dependent manner, and the decreased SPR signals were reversible when the exposure was drawn off. However, MF pre-exposed culture medium did not significantly change cell viability, intracellular reactive oxygen species level, activation of the MARK signaling pathways, or genetic stabilities in human amniotic epithelial cells (FL cells). In conclusion, our data suggest that the relative permittivity of culture medium was influenced by 50 Hz MF exposure, but this change did not affect the biological processes in FL cells.

Osteogenic differentiation of equine cord blood multipotent mesenchymal stromal cells within coralline hydroxyapatite scaffolds in vitro

2011 ◽  
Vol 24 (05) ◽  
pp. 354-362 ◽  
Author(s):  
R. J. Figueroa ◽  
T. G. Koch ◽  
D. H. Betts
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Viability ◽  
Osteogenic Differentiation ◽  
Alkaline Phosphatase Activity ◽  
Stromal Cells ◽  
Culture Medium ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Coralline Hydroxyapatite ◽  
Osteogenic Induction

SummaryObjective: To investigate the osteogenic differentiation potential of equine umbilical cord blood-derived multipotent mesenchymal stromal cells (CB-MSC) within coralline hydro-xyapatite scaffolds cultured in osteogenic induction culture medium.Methods: Scaffolds seeded with equine CBMSC were cultured in cell expansion culture medium (control) or osteogenic induction medium (treatment). Cell viability and distribution were confirmed by the MTT cell viability assay and DAPI nuclear fluorescence staining, respectively. Osteogenic differentiation was evaluated after 10 days using reverse transcription polymerase chain reaction, alkaline phosphatase activity, and secreted osteocalcin concentration. Cell morphology and matrix deposition were assessed by scanning electron microscopy (SEM) after 14 days in culture.Results: Cells showed viability and adequate distribution within the scaffold. Successful osteogenic differentiation within the scaffolds was demonstrated by the increased expression of osteogenic markers such as Runx2, osteopontin, osteonectin, collagen IA increased levels of alkaline phosphatase activity increased osteocalcin protein secretion and bone-like matrix presence in the scaffold pores upon SEM evaluation.Clinical significance: These results demonstrate that equine CB-MSC maintain viability and exhibit osteogenic potential in coralline hydroxyapatite scaffolds when induced in vitro. Equine CB-MSC scaffold constructs deserve further investigation for their potential role as biologically active fillers to enhance bone-gap repair in the horse.

In vitroeffects of ghrelin on gastric H+-K+-ATPase and pepsin activity and mRNA expression of gastrin, somatostatin, receptors for GH and IGF-1 in cultured gastric mucosal cells of weanling piglets

2006 ◽  
Vol 82 (6) ◽  
pp. 823-828 ◽  
Author(s):  
G.-M. Du ◽  
M.-J. Liu ◽  
Z.-M. Shi ◽  
L. Zhang ◽  
X.-H. Wei ◽  
...  
Keyword(s):  
Atpase Activity ◽  
Cell Viability ◽  
Mrna Expression ◽  
Culture Medium ◽  
Somatostatin Receptors ◽  
Gastric Mucosal Cells ◽  
Pepsin Activity ◽  
Mucosal Cells ◽  
Weanling Piglets

AbstractThe present study was conducted to investigate the effects of ghrelin on gastric acid and pepsin secretion, as well as the mRNA expression of gastrin, somatostatin (SS) and receptors for growth hormone (GH) and insulin-like growth factor-1 (IGF-1) in gastric mucosal cells in vitro. Gastric mucosal cells were isolated from 5-week-old weanling piglets and exposed for 4 h to 3×10−2, 3×10−1, 3, 3×10 and 3×102nmol/l of h-ghrelin, respectively after 30-h incubation in DMEM/F-12. Pepsin activity in culture medium, cell viability and H+-K+-ATPase activity, as well as mRNA expression of gastrin, SS, GHR and IGF-1R in gastric mucosal cells were determined as response criteria. The experiment was repeated three times. Ghrelin significantly (P<0·05) increased H+-K+-ATPase activity of gastric mucosal cells at 3×10−1, 3 and 3×10 nmol/l of h-ghrelin. However, no significant changes were observed either in pepsin activity or the cell viability after ghrelin treatment. The mRNA expression of gastrin and SS was significantly increased in gastric mucosal cells exposed to h-ghrelin at 3×10−1and 3 nmol/l (P<0·05). H-ghrelin significantly increased IGF-1R but not GHR mRNA expression at 3×10−1, 3 and 3×10 nmol/l of h-ghrelin (P<0·05). Ghrelin acts on gastric mucosal cells from weanling piglets to regulate the H+-K+-ATPase activity and mRNA expression of gastrin, SS, and IGF-1Rin vitro.

scholarly journals Solvent and HEMA Increase Adhesive Toxicity and Cytokine Release from Dental Pulp Cells

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2750 ◽  
Author(s):  
Helder Massaro ◽  
Lígia Zambelli ◽  
Auriléia Britto ◽  
Rodolfo Vieira ◽  
Ana Ligeiro-de-Oliveira ◽  
...  
Keyword(s):  
Cell Viability ◽  
Dental Pulp ◽  
Culture Medium ◽  
Control Group ◽  
Dental Pulp Cells ◽  
Cytokine Release ◽  
Human Dental Pulp Cells ◽  
Medium Components ◽  
Tnf Α ◽  
Pulp Cells

The aim of the present study was to evaluate the effect of the hydroxyethyl-methacrylate (HEMA) concentration and solvent content of dental adhesives on cell viability and cytokine (IL-1b, IL-6, IL-10, TNF-α) release by human dental pulp cells (HDPCs). HDPCs were obtained from fresh extracted human third molars. Experimental adhesives were prepared containing different concentrations of HEMA (0%, 10%, and 20%) with and without solvent (ethanol 10%). Cylindrical specimens were immersed on culture medium during 24 h to obtain the extracts. The cells were incubated with extracts (culture medium + components leached from the adhesives) of different adhesives, and cell viability and cytokine release were evaluated after 6 and 24 h of exposure. Adhesives containing HEMA promoted high cell viability reduction after 6 h of exposure; but after 24 h, the results were similar to the ones found among control group cells. These effects on cell viability were prominently increased with the addition of solvent. Although IL-1b release was not affected by exposure to eluates, other cytokines (IL-10, IL-6, TNF-α) were modulated by the different experiment conditions, directly influenced by the HEMA concentration and presence of solvent. Higher HEMA concentrations, combined with the presence of solvent, can promote significant reduction on HDPC viability, increasing the release of anti- and pro-inflammatory mediators.

Forskolin-Stimulated Adenylylcyclase Activity: A Marker to Assess Islet Cell Viability Following Cold Storage in Different Solutions and to Predict Islet Cell Function following Transplantation

1999 ◽  
Vol 8 (4) ◽  
pp. 383-388 ◽  
Author(s):  
Shigeo Kanazawa ◽  
Tsukasa Tsunoda ◽  
Masakazu Murakami ◽  
Sueharu Iwamoto ◽  
Masatoshi Kimoto ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cold Storage ◽  
Islet Cell ◽  
Cell Function ◽  
Islet Cell Function

scholarly journals Cytotoxicity of Etch-and-Rinse, Self-Etch, and Universal Dental Adhesive Systems in Fibroblast Cell Line 3T3

Scanning ◽  
2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Yasmine Mendes Pupo ◽  
Cintia Fernanda de Freitas Bernardo ◽  
Francielly Fernanda de Freitas A. de Souza ◽  
Milton Domingos Michél ◽  
Camila Nunes de Morais Ribeiro ◽  
...  
Keyword(s):  
Cell Viability ◽  
Culture Medium ◽  
Fibroblast Cell ◽  
Adhesive System ◽  
Negative Control ◽  
Adhesive Systems ◽  
Universal Adhesive ◽  
Glass Cover ◽  
Etch And Rinse ◽  
Self Etch

The aim of this study was to evaluate in fibroblast cultures the direct cytotoxic effects of etch-and-rinse, self-etch, and universal adhesive systems. The sterile glass cover slips (n=3) were then immersed in culture medium to obtain the eluates for the experimental groups: (1) Adper™ Single Bond 2; (2) Ambar; (3) Adper™ Scotchbond™ Multi-Purpose; (4) Scotchbond™ Universal; (5) Ambar Universal; and (6) OptiBond All-In-One. As a negative control, sterile glass cover slips were immersed in culture medium only. After 24 h, the eluate obtained was applied on fibroblast culture. Cell viability and cell morphology were evaluated by MTT assay and SEM, respectively. Data were analyzed by Kruskal–Wallis and Mann–Whitney tests (α=0.05). All adhesive systems except universal reduced cell viability in 3T3 cells to between 26.04% and 56.57%, and Scotchbond Universal and Ambar Universal reduced cell viability to 2.13% and 3.57%, respectively, when compared to the negative control. Cytoplasmic membrane shrinkage and cell-free areas with residual membrane fragments from dead cells were observed. In conclusion, improvements in universal adhesive system formulations and their mechanisms of action are not accompanied by increased toxicity compared with those in other systems, warranting commitment to the use of these dentin-pulp complexes.

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