P0571 / #307: ASSOCIATION BETWEEN SEVERE ACUTE RESPIRATORY INFECTION BY HUMAN METAPNEUMOVIRUS, RAINFALL SEASON AND CLINICAL OUTCOMES IN CHILDREN

Abstract Background Human metapneumovirus (hMPV) is one of the important pathogens in infant respiratory tract infection. However, the molecular epidemiology of hMPV among children < 14 years of age hospitalized with severe acute respiratory infection (SARI) is unclear. We investigated the hMPV infection status and genotypes of children hospitalized with SARI from January 2016 to December 2020 in Huzhou, China. Methods A nasopharyngeal flocked swab, nasal wash, or nasopharyngeal swab/or opharyngeal swab combination sample was collected from children with SARI in Huzhou from January 2016 to December 2020. Quantitative reverse transcription-polymerase chain reaction was performed to detect hMPV RNA. The hMPV F gene was amplified and sequenced, followed by analysis using MEGA software (ver. 7.0). Epidemiological data were analyzed using Microsoft Excel 2010 and SPSS (ver. 22.0) software. Results A total of 1133 children with SARI were recruited from 2016 to 2020. Among them, 56 (4.94%) were positive for hMPV-RNA. Children < 5 years of age accounted for 85.71% of the positive cases. The hMPV incidence was high in spring and winter, especially in December and January to March. Phylogenetic analysis of the F-gene sequences of 28 hMPV strains showed that the A1, B1, and B2 genotypes were prevalent in Huzhou, and the dominant hMPV genotype varied according to surveillance year. Conclusions HMPV is an important respiratory pathogen in children in Huzhou, with a high incidence in winter and spring in children < 5 years of age. In this study, genotypes A1, B1, and B2 were the most prevalent.

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Emerging Human Metapneumovirus Gene Duplication Variants in Patients with Severe Acute Respiratory Infection, China, 2017–2019

Emerging Infectious Diseases ◽

10.3201/eid2701.201043 ◽

2021 ◽

Vol 27 (1) ◽

pp. 275-277

Author(s):

Zhibo Xie ◽

Jin Xu ◽

Yunhui Ren ◽

Aili Cui ◽

Huiling Wang ◽

...

Keyword(s):

Gene Duplication ◽

Respiratory Infection ◽

Acute Respiratory Infection ◽

Human Metapneumovirus ◽

Severe Acute Respiratory Infection

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Molecular Epidemiology of Human Metapneumovirus Infection among Children with Severe Acute Respiratory Infection in Huzhou from 2016 to 2020

10.21203/rs.3.rs-578670/v1 ◽

2021 ◽

Author(s):

Lei Ji ◽

Liping Chen ◽

deshun xu ◽

Xiaofang Wu

Keyword(s):

Nucleic Acid ◽

Respiratory Infection ◽

Acute Respiratory Infection ◽

Human Metapneumovirus ◽

Hospitalized Patients ◽

Nasopharyngeal Swab ◽

Gene Sequences ◽

F Gene ◽

Severe Acute Respiratory Infection ◽

Hmpv Infection

Abstract Background: Human metapneumovirus (hMPV) is one of the important pathogens of infant respiratory tract infection. However, the molecular epidemiological information relating to hMPV among children hospitalized patients with severe acute respiratory infection (SARI) have not been thoroughly studied as yet. To investigate the infection status and genotypes of hMPV among children hospitalized patients with SARI from January 2016 to December 2020 in Huzhou, China. Methods: A nasopharyngeal flocked swab, nasal wash or combination of nasopharyngeal swab and oropharyngeal swab samples were collected from children with SARI in Huzhou from January 2016 to December 2020. RT-PCR was used to detect the nucleic acid of hMPV. F gene was amplified and sequenced for the positive nucleic acid samples of hMPV. The obtained gene sequences were analyzed by MEGA software (version 7.0). Epidemiological data were analyzed using Microsoft Excel 2010 and service solutions (SPSS) 21.0 software.Results: A total of 1133 children with SARI were collected from 2016 to 2020. Among them, 56 cases were positive for hMPV nucleic acid, with a positive rate of 4.94%. Children under 5 years old accounted for 85.71% of the total positive cases. Higher activity of hMPV infection could be seen in the period in Spring and Winter. and the main epidemic months were December and January-March. The F gene sequences of 28 strains of hMPV were obtained by co-sequencing. Phylogenetic analysis showed that there were A1, B1 and B2 genotypes of hMPV prevalent in Huzhou, and the dominant genotype of hMPV during our study period varied according to surveillance year.Conclusions: HMPV is one of the important pathogens causing acute respiratory virus infection in children in Huzhou, with high incidence in winter and spring seasons and children under the age of 5，A1, B1 and B2 are three prevalent genotypes.

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Molecular Typing and Epidemiology Profiles of Human Metapneumovirus Infection Among Children With Severe Acute Respiratory Infection in Huzhou, China

10.21203/rs.3.rs-538269/v2 ◽

2021 ◽

Author(s):

Lei Ji ◽

Liping Chen ◽

Deshun Xu ◽

Xiaofang Wu

Keyword(s):

Nucleic Acid ◽

Respiratory Infection ◽

Acute Respiratory Infection ◽

Human Metapneumovirus ◽

Hospitalized Patients ◽

Nasopharyngeal Swab ◽

Gene Sequences ◽

F Gene ◽

Severe Acute Respiratory Infection ◽

Hmpv Infection

Abstract Background: Human metapneumovirus (hMPV) is one of the important pathogens of infant respiratory tract infection. However, the molecular epidemiological information relating to hMPV among children hospitalized patients with severe acute respiratory infection (SARI) have not been thoroughly studied as yet. To investigate the infection status and genotypes of hMPV among children hospitalized patients with SARI from January 2016 to December 2020 in Huzhou, China. Methods: A nasopharyngeal flocked swab, nasal wash or combination of nasopharyngeal swab and oropharyngeal swab samples were collected from children with SARI in Huzhou from January 2016 to December 2020. RT-PCR was used to detect the nucleic acid of hMPV. F gene was amplified and sequenced for the positive nucleic acid samples of hMPV. The obtained gene sequences were analyzed by MEGA software (version 7.0). Epidemiological data were analyzed using Microsoft Excel 2010 and service solutions (SPSS) 21.0 software.Results: A total of 1133 children with SARI were collected from 2016 to 2020. Among them, 56 cases were positive for hMPV nucleic acid, with a positive rate of 4.94%. Children under 5 years old accounted for 85.71% of the total positive cases. Higher activity of hMPV infection could be seen in the period in Spring and Winter. and the main epidemic months were December and January-March. The F gene sequences of 28 strains of hMPV were obtained by co-sequencing. Phylogenetic analysis showed that there were A1, B1 and B2 genotypes of hMPV prevalent in Huzhou, and the dominant genotype of hMPV during our study period varied according to surveillance year.Conclusions: HMPV is one of the important pathogens causing acute respiratory virus infection in children in Huzhou, with high incidence in winter and spring seasons and children under the age of 5，A1, B1 and B2 are three prevalent genotypes.

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Prevalence, clinical outcomes and rainfall association of acute respiratory infection by human metapneumovirus in children in Bogotá, Colombia

BMC Pediatrics ◽

10.1186/s12887-019-1734-x ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 1

Author(s):

Obando Evelyn ◽

Fernández-Sarmiento Jaime ◽

Montoya David ◽

Acevedo Lorena ◽

Arroyave Jenifer ◽

...

Keyword(s):

Clinical Outcomes ◽

Respiratory Infection ◽

Acute Respiratory Infection ◽

Respiratory Infections ◽

Human Metapneumovirus ◽

Acute Respiratory Infections ◽

Rt Pcr ◽

Clinical Behavior ◽

Hmpv Infection ◽

Viral Coinfection

Abstract Background Acute respiratory infections (ARIs) are one of the main causes of morbidity and mortality in children. Viruses are the main etiological agents, and their behavior tends to be seasonal and vary by geographical location. Human metapneumovirus (HMPV) has recently been described as a cause of severe acute respiratory infection and its prevalence and clinical behavior in children at moderate altitudes is unknown. Methods A cross-sectional study was carried out on patients seen at a university hospital in Bogotá, Colombia between October 2015 and December 2017 in a city at a moderate altitude above sea level. Children with acute respiratory infections who had had a multiplex RT-PCR assay were selected. The prevalence of HMPV infection, its clinical outcomes and its relationship to rainfall were evaluated. Results Out of a total of 14,760 discharged patients, multiplex RT-PCR was performed on 502 and a virus was detected in 420 children with acute respiratory infection (ARI). The study group had a median age of 21 months (IQR 7–60), with similar proportion of males and females (56.4 and 43.6% respectively) and 5.2% (CI 95 3.3–7.8%) prevalence of HMPV infection. The group with HMPV infection showed a greater frequency of viral coinfection (22.7% vs 14% P = 0.03) compared with ARI caused by other viruses. The rate of bacterial coinfection (P = 0.31), presence of comorbidities (p = 0.75), length of hospital stay (P = 0.42), need for mechanical ventilation (P = 0.75) and mortality (P = 0.22) were similar for HMPV and other viral infections. A moderate correlation was established between HMPV infection and rainfall peaks (Spearman’s Rho 0.44 p = 0.02). Conclusions Human metapneumovirus was the fifth most frequently isolated virus in children with ARI, had similar clinical behavior and severity to other viruses but a higher rate of viral coinfection. Its peaks seem to correlate to rainy seasons.

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Viral causes of severe acute respiratory infection in hospitalized children and association with outcomes: A two-year prospective surveillance study in Suriname

PLoS ONE ◽

10.1371/journal.pone.0247000 ◽

2021 ◽

Vol 16 (2) ◽

pp. e0247000

Author(s):

Amadu E. Juliana ◽

Ming-Jan Tang ◽

Lex Kemps ◽

Albert C. Noort ◽

Sandra Hermelijn ◽

...

Keyword(s):

Developing Countries ◽

Clinical Outcomes ◽

Respiratory Infection ◽

Acute Respiratory Infection ◽

Respiratory Support ◽

Specific Patient ◽

Course Of Disease ◽

Clinical Variables ◽

Severe Acute Respiratory Infection ◽

Syncytial Virus

Background Viruses are the most frequent cause of severe acute respiratory infection (SARI) in children. It is currently unknown whether presence of a virus, the number of viruses, or type of virus, are associated with clinical outcomes of pediatric SARI in developing countries. Methods Between 2012 and 2014 nasopharyngeal swabs and demographic and clinical variables were prospectively collected for surveillance of viral causes of SARI in Surinamese children within 48 hours after hospitalization. These swabs were tested for 18 respiratory viruses using a multiplex polymerase chain reaction (PCR) panel to identify the specific viral causes of SARI, unknown to the treating physicians. In post hoc analyses we evaluated if the PCR results, and demographic and clinical characteristics, were associated with course of disease, duration of respiratory support, and length of stay (LOS). Results Of a total of 316 analyzed children, 290 (92%) had one or more viruses. Rhinovirus/enterovirus (43%) and respiratory syncytial virus (34%) were most prevalent. Course of disease was mild in 234 (74%), moderate in 68 (22%), and severe in 14 (4%) children. Neither presence of a single virus, multiple viruses, or the type of virus, were different between groups. Prematurity and lower weight-for-age-z-score were independent predictors of a severe course of disease, longer duration of respiratory support, and longer LOS. Conclusions Viruses are common causes of pediatric SARI in Suriname, yet not necessarily associated with clinical outcomes. In developing countries, demographic and clinical variables can help to identify children at-risk for worse outcome, while PCR testing may be reserved to identify specific viruses, such as influenza, in specific patient groups or during outbreaks.

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