scholarly journals Transmural impedance detects graded changes of inflammation in experimental colitis

2020 ◽  
Vol 7 (2) ◽  
pp. 191819
Author(s):  
Sophie C. Payne ◽  
Jack Alexandrovics ◽  
Ross Thomas ◽  
Robert K. Shepherd ◽  
John B. Furness ◽  
...  
Keyword(s):  
Normal Tissue ◽  
Electrical Impedance ◽  
Intestinal Inflammation ◽  
Experimental Colitis ◽  
Endoscopic Examination ◽  
Qualitative Assessment ◽  
Impedance Measurements ◽  
Mucosal Integrity ◽  
Histological Damage ◽  
Trinitrobenzene Sulphonic Acid

Ulcerative colitis is a chronic disease in which the mucosa of the colon or rectum becomes inflamed. An objective biomarker of inflammation will provide quantitative measures to support qualitative assessment during an endoscopic examination. Previous studies show that transmural electrical impedance is a quantifiable biomarker of inflammation. Here, we hypothesize that impedance detects spatially restricted areas of inflammation, thereby allowing the distinction between regions that differ in their severity of inflammation. A platinum ball electrode was placed into minimally inflamed (i.e. normal) or 2,4,6-trinitrobenzene sulphonic acid (TNBS)-inflamed colonic regions of rats and impedance measurements obtained by passing current between the intraluminal and subcutaneous return electrode. Histology of the colon was correlated with impedance measurements. The impedance of minimally inflamed (normal) tissue was 1.5–1.9 kΩ. Following TNBS injection, impedance significantly decreased within the inflammatory penumbra ( p < 0.05), and decreased more in the inflammatory epicentre ( p = 0.02). Histological damage correlated with impedance values ( p < 0.05). Thus, impedance values of 1.5–1.9, 1.3–1.4 and 0.9–1.1 kΩ corresponded to minimally inflamed, mildly inflamed and moderately inflamed tissue, respectively. In conclusion, transmural impedance is an objective, spatially localized biomarker of mucosal integrity, and distinguishes between severities of intestinal inflammation.

scholarly journals Loss of Nckx3 Exacerbates Experimental DSS-Induced Colitis in Mice through p53/NF-κB Pathway

2021 ◽  
Vol 22 (5) ◽  
pp. 2645
Author(s):  
Dinh Nam Tran ◽  
Seon Myeong Go ◽  
Seon-Mi Park ◽  
Eui-Man Jung ◽  
Eui-Bae Jeung
Keyword(s):  
Immune Response ◽  
Cellular Proliferation ◽  
Intestinal Inflammation ◽  
Critical Role ◽  
Experimental Colitis ◽  
Innate Immune ◽  
Inflammatory Conditions ◽  
Bowel Diseases ◽  
Inflammatory Bowel ◽  
Microarray Analyses

Inflammatory bowel diseases (IBDs) comprises a range of chronic inflammatory conditions of the intestinal tract. The incidence and prevalence of IBDs are increasing worldwide, but the precise etiology of these diseases is not completely understood. Calcium signaling plays a regulatory role in cellular proliferation. Nckx3, a potassium-dependent Na+/Ca2+ exchanger, is not only expressed in the brain but also in the aortic, uterine, and intestinal tissues, which contain abundant smooth muscle cells. This study investigated the role of Nckx3 in intestinal inflammation. Microarray analyses revealed the upregulation of the innate immune response-associated genes in the duodenum of Nckx3 knockout (KO) mice. The Nckx3 KO mice also showed an increase in IBD- and tumorigenesis-related genes. Using dextran sodium sulfate (DSS)-induced experimental colitis mice models, the Nckx3 KO mice showed severe colitis. Furthermore, the pathways involving p53 and NF-κB signaling were significantly upregulated by the absence of Nckx3. Overall, Nckx3 plays a critical role in the innate immune and immune response and may be central to the pathogenesis of IBD.

scholarly journals Dry Film Resist Laminated Microfluidic System for Electrical Impedance Measurements

Micromachines ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 632
Author(s):  
Yuan Cao ◽  
Julia Floehr ◽  
Sven Ingebrandt ◽  
Uwe Schnakenberg
Keyword(s):  
Microfluidic Chip ◽  
Electrical Impedance ◽  
Microfluidic System ◽  
Equivalent Circuits ◽  
Proof Of Concept ◽  
Impedance Measurements ◽  
Close Match ◽  
Aspect Ratios ◽  
Lamination Process ◽  
Dry Film

In micro-electrical-mechanical systems (MEMS), thick structures with high aspect ratios are often required. Dry film photoresist (DFR) in various thicknesses can be easily laminated and patterned using standard UV lithography. Here, we present a three-level DFR lamination process of SUEX for a microfluidic chip with embedded, vertically arranged microelectrodes for electrical impedance measurements. To trap and fix the object under test to the electrodes, an aperture is formed in the center of the ring-shaped electrodes in combination with a microfluidic suction channel underneath. In a proof-of-concept, the setup is characterized by electrical impedance measurements with polystyrene and ZrO2 spheres. The electrical impedance is most sensitive at approximately 2 kHz, and its magnitudes reveal around 200% higher values when a sphere is trapped. The magnitude values depend on the sizes of the spheres. Electrical equivalent circuits are applied to simulate the experimental results with a close match.

Gluten intake increases bacterial translocation and aggravates intestinal inflammation in experimental colitis

2018 ◽  
Vol 37 ◽  
pp. S69
Author(s):  
P.L. Menta ◽  
M.E. Andrade ◽  
P. Leocardio ◽  
J. Fraga ◽  
M. Silva ◽  
...  
Keyword(s):  
Bacterial Translocation ◽  
Intestinal Inflammation ◽  
Experimental Colitis

Electrical impedance measurements for pulmonary disease diagnosis

1992 ◽  
Vol 13 (A) ◽  
pp. 171-174 ◽  
Author(s):  
J N Sahalos ◽  
E Vlachogiannis ◽  
C Koukourlis ◽  
G Kyriacou ◽  
K Batas
Keyword(s):  
Pulmonary Disease ◽  
Electrical Impedance ◽  
Disease Diagnosis ◽  
Impedance Measurements

scholarly journals Low Cost Instrumentation for Electrical Impedance Tomography in Biomedical Applications

2015 ◽  
Author(s):  
Stewart Smith ◽  
Hancong Wu ◽  
Jiabin Jia
Keyword(s):  
Electrical Impedance Tomography ◽  
Integrated Circuit ◽  
Biomedical Applications ◽  
Electrical Impedance ◽  
Low Cost ◽  
Impedance Measurement ◽  
Impedance Tomography ◽  
Impedance Measurements ◽  
Additional Circuitry ◽  
Initial Results

This poster reports the design, implementation and testing of a portable and inexpensive bio-impedance measurement system intended for electrical impedance tomography (EIT) in cell cultures. The system is based on the AD5933 impedance analyser integrated circuit with additional circuitry to enable four-terminal measurement. Initial results of impedance measurements are reported along with an EIT image reconstructed using the open source EIDORS package.

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