scholarly journals Thalassomonas agarivorans sp. nov., a marine agarolytic bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the genus Thalassomonas

2006 ◽  
Vol 56 (6) ◽  
pp. 1245-1250 ◽  
Author(s):  
Wen Dar Jean ◽  
Wung Yang Shieh ◽  
Tung Yen Liu
Keyword(s):  
Novel Species ◽  
Optimal Growth ◽  
Anaerobic Growth ◽  
The Novel ◽  
Cellular Fatty Acids ◽  
Water Region ◽  
The Family ◽  
Emended Description ◽  
Curved Rods ◽  
Shallow Water Region

A marine agarolytic bacterium, designated strain TMA1T, was isolated from a seawater sample collected in a shallow-water region of An-Ping Harbour, Taiwan. It was non-fermentative and Gram-negative. Cells grown in broth cultures were straight or curved rods, non-motile and non-flagellated. The isolate required NaCl for growth and exhibited optimal growth at 25 °C and 3 % NaCl. It grew aerobically and was incapable of anaerobic growth by fermenting glucose or other carbohydrates. Predominant cellular fatty acids were C16 : 0 (17.5 %), C17 : 1 ω8c (12.8 %), C17 : 0 (11.1 %), C15 : 0 iso 2-OH/C16 : 1 ω7c (8.6 %) and C13 : 0 (7.3 %). The DNA G+C content was 41.0 mol%. Phylogenetic, phenotypic and chemotaxonomic data accumulated in this study revealed that the isolate could be classified in a novel species of the genus Thalassomonas in the family Colwelliaceae. The name Thalassomonas agarivorans sp. nov. is proposed for the novel species, with TMA1T (=BCRC 17492T=JCM 13379T) as the type strain.

scholarly journals Pseudidiomarina taiwanensis gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the family Idiomarinaceae

2006 ◽  
Vol 56 (4) ◽  
pp. 899-905 ◽  
Author(s):  
Wen Dar Jean ◽  
Wung Yang Shieh ◽  
Hsiu-Hui Chiu
Keyword(s):  
Marine Bacterium ◽  
Sea Water ◽  
Coastal Region ◽  
Optimal Growth ◽  
Anaerobic Growth ◽  
Rrna Gene ◽  
The Novel ◽  
Novel Genus ◽  
The Family ◽  
Emended Description

Two strains of heterotrophic, aerobic, marine bacteria, designated strains PIT1T and PIT2, were isolated from sea-water samples collected at the shallow coastal region of An-Ping Harbour, Tainan, Taiwan. Both strains were Gram-negative. Cells grown in broth cultures were straight rods that were non-motile, lacking flagella. Both strains required NaCl for growth and exhibited optimal growth at 30–35 °C, 1–4 % NaCl and pH 8. They grew aerobically and were incapable of anaerobic growth by fermentation of glucose or other carbohydrates. Cellular fatty acids were predominantly iso-branched, with C15 : 0 iso and C17 : 0 iso representing the most abundant components. The DNA G+C contents of strains PIT1T and PIT2 were 49·3 and 48·6 mol%, respectively. Phylogeny based on 16S rRNA gene sequences, together with data from phenotypic and chemotaxonomic characterization, revealed that the two isolates could be assigned to a novel genus in the family Idiomarinaceae, for which the name Pseudidiomarina gen. nov. is proposed. Pseudidiomarina taiwanensis sp. nov. is the type species of the novel genus (type strain PIT1T=BCRC 17465T=JCM 13360T).

scholarly journals Three Planctomycetes isolated from biotic surfaces in the Mediterranean Sea and the Pacific Ocean constitute the novel species Symmachiella dynata gen. nov., sp. nov. and Symmachiella macrocystis sp. nov.

2020 ◽  
Vol 113 (12) ◽  
pp. 1965-1977
Author(s):  
Markus Salbreiter ◽  
Muhammad Waqqas ◽  
Mareike Jogler ◽  
Nicolai Kallscheuer ◽  
Sandra Wiegand ◽  
...  
Keyword(s):  
Cell Biology ◽  
Novel Species ◽  
Optimal Growth ◽  
Monterey Bay ◽  
The Novel ◽  
Separate Species ◽  
The Pacific ◽  
The Family ◽  
The Pacific Ocean ◽  
Significant Attention

AbstractPlanctomycetes is a phylum of environmentally important bacteria, which also receive significant attention due to their fascinating cell biology. Access to axenic Planctomycete cultures is crucial to study cell biological features within this phylum in further detail. In this study, we characterise three novel strains, Mal52T, Pan258 and CA54T, which were isolated close to the coasts of the islands Mallorca (Spain) and Panarea (Italy), and from Monterey Bay, CA, USA. The three isolates show optimal growth at temperatures between 22 and 24 °C and at pH 7.5, divide by polar budding, lack pigmentation and form strong aggregates in liquid culture. Analysis of five phylogenetic markers suggests that the strains constitute two novel species within a novel genus in the family Planctomycetaceae. The strains Mal52T (DSM 101177T = VKM B-3432T) and Pan258 were assigned to the species Symmachiella dynata gen nov., sp. nov., while strain CA54T (DSM 104301T = VKM B-3450T) forms a separate species of the same genus, for which we propose the name Symmachiella macrocystis sp. nov.

scholarly journals Rosistilla oblonga gen. nov., sp. nov. and Rosistilla carotiformis sp. nov., isolated from biotic or abiotic surfaces in Northern Germany, Mallorca, Spain and California, USA

2020 ◽  
Vol 113 (12) ◽  
pp. 1939-1952 ◽  
Author(s):  
Muhammad Waqqas ◽  
Markus Salbreiter ◽  
Nicolai Kallscheuer ◽  
Mareike Jogler ◽  
Sandra Wiegand ◽  
...  
Keyword(s):  
Cell Biology ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Optimal Growth ◽  
The Novel ◽  
Biological Features ◽  
Abiotic Surfaces ◽  
The Family ◽  
Axenic Cultures ◽  
The Baltic

AbstractPlanctomycetes are ubiquitous bacteria with fascinating cell biological features. Strains available as axenic cultures in most cases have been isolated from aquatic environments and serve as a basis to study planctomycetal cell biology and interactions in further detail. As a contribution to the current collection of axenic cultures, here we characterise three closely related strains, Poly24T, CA51T and Mal33, which were isolated from the Baltic Sea, the Pacific Ocean and the Mediterranean Sea, respectively. The strains display cell biological features typical for related Planctomycetes, such as division by polar budding, presence of crateriform structures and formation of rosettes. Optimal growth was observed at temperatures of 30–33 °C and at pH 7.5, which led to maximal growth rates of 0.065–0.079 h−1, corresponding to generation times of 9–11 h. The genomes of the novel isolates have a size of 7.3–7.5 Mb and a G + C content of 57.7–58.2%. Phylogenetic analyses place the strains in the family Pirellulaceae and suggest that Roseimaritima ulvae and Roseimaritima sediminicola are the current closest relatives. Analysis of five different phylogenetic markers, however, supports the delineation of the strains from members of the genus Roseimaritima and other characterised genera in the family. Supported by morphological and physiological differences, we conclude that the strains belong to the novel genus Rosistilla gen. nov. and constitute two novel species, for which we propose the names Rosistilla carotiformis sp. nov. and Rosistilla oblonga sp. nov. (the type species). The two novel species are represented by the type strains Poly24T (= DSM 102938T = VKM B-3434T = LMG 31347T = CECT 9848T) and CA51T (= DSM 104080T = LMG 29702T), respectively.

scholarly journals Saccharicrinis marinus sp. nov., isolated from marine sediment

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3427-3432 ◽  
Author(s):  
Qian-Qian Liu ◽  
Juan Li ◽  
Di Xiao ◽  
Jin-Xing Lu ◽  
Guan-Jun Chen ◽  
...  
Keyword(s):  
Marine Sediment ◽  
Type Strain ◽  
Novel Species ◽  
Optimal Growth ◽  
Rrna Gene ◽  
The Novel ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Cellular Fatty Acids ◽  
Motile By Gliding

A novel bacterial strain, designated Y11T, was isolated from marine sediment at Weihai in China. Comparative analysis of 16S rRNA gene sequences demonstrated that the novel isolate showed highest similarity to Saccharicrinis fermentans DSM 9555T (94.0 %) and Saccharicrinis carchari SS12T (92.7 %). Strain Y11T was a Gram-stain-negative, rod-shaped, non-endospore-forming, yellow-pigmented bacterium and was able to hydrolyse agar weakly. It was catalase-negative, oxidase-positive, facultatively anaerobic and motile by gliding. Optimal growth occurred at 28–30 °C, at pH 7.0–7.5 and in the presence of 2–3 % (w/v) NaCl. The DNA G+C content was 34.4 mol%. The strain contained MK-7 as the prevalent menaquinone. The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C15 : 1ω6c. The predominant polar lipids were phosphatidylethanolamine and two unknown lipids. Data from the present polyphasic taxonomic study clearly place the strain as representing a novel species within the genus Saccharicrinis, for which the name Saccharicrinis marinus sp. nov. is proposed. The type strain is Y11T ( = CICC10837T = KCTC42400T).

scholarly journals Idiomarina aquatica sp. nov., a moderately halophilic bacterium isolated from salterns

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4595-4600 ◽  
Author(s):  
María José León ◽  
Fernando Martínez-Checa ◽  
Antonio Ventosa ◽  
Cristina Sánchez-Porro
Keyword(s):  
Novel Species ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
The Novel ◽  
Bacterial Strains ◽  
Moderately Halophilic Bacterium ◽  
Curved Rods ◽  
Content Range

Four bacterial strains, SN-14T, SN-4, M6-46 and M6-58B, were isolated from water of ponds of two salterns located in Huelva (Spain). They were Gram-stain-negative, aerobic and slightly curved rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the four strains belong to the genus Idiomarina, being related most closely to Idiomarina fontislapidosi F23T (98.4–98.0% sequence similarity), Idiomarina seosinensis CL-SP19T (98.3–98.0%), Idiomarina piscisalsi TPS4-2T (97.9–97.4%), Idiomarina baltica OS145T (97.5–97.4%) and Idiomarina zobellii KMM 231T (97.6–97.0%). The level of similarity with the type species of the genus, Idiomarina abyssalis KMM 227T, was 97.2–96.7%. The novel strains exhibited optimal growth at 5–10% (w/v) total salts, pH 7 and 37 °C. The major fatty acids of strain SN-14T were iso-C15 : 0, iso-C17 : 0, C18 : 1ω7c/C18 : 1ω6c, C16 : 0 and iso-C17 : 1ω9c/C16 : 0 10-methyl. The DNA G+C content range was 47.6–50.8 mol%. The level of DNA–DNA relatedness between strain SN-14T and I. fontislapidosi F23T was 13%, while those between strain SN-14T and the other three new isolates were between 77 and 99%. These data demonstrated that the four isolates constitute a novel species of the genus Idiomarina. Based on the phylogenetic, genotypic, phenotypic and chemotaxonomic data, the four strains represent a novel species of the genus Idiomarina, for which the name Idiomarina aquatica sp. nov. is proposed. The type strain is SN-14T ( = CCM 8471T = CECT 8360T = LMG 27613T).

scholarly journals Neptunomonas concharum sp. nov., isolated from a dead ark clam, and emended description of the genus Neptunomonas

2012 ◽  
Vol 62 (Pt_11) ◽  
pp. 2657-2661 ◽  
Author(s):  
Hae-Won Lee ◽  
Na-Ri Shin ◽  
Jina Lee ◽  
Seong Woon Roh ◽  
Tae Woong Whon ◽  
...  
Keyword(s):  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
The Novel ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
Gram Staining ◽  
Emended Description

A novel Gram-staining-negative, facultatively anaerobic, motile and rod-shaped bacterium, designated strain LHW37T, was isolated from a dead ark clam collected on the south coast of Korea. The novel strain grew optimally at 37 °C, at pH 7.0–8.0 and with 2 % (w/v) NaCl. The predominant cellular fatty acids were C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The major isoprenoid quinone was ubiquinone-8 (Q-8) and the predominant polar lipids were phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain was most closely related to Neptunomonas japonica JAMM 0745T (97.1 % sequence similarity). The genomic DNA G+C content of strain LHW37T was 48.2 mol%. The DNA–DNA relatedness values recorded in hybridization experiments between the novel strain and its closest known relative were ≤18 %. Based on the phenotypic, genotypic and phylogenetic data, strain LHW37T represents a novel species belonging to the genus Neptunomonas for which the name Neptunomonas concharum sp. nov. is proposed. The type strain is LHW37T ( = KACC 15543T  = JCM 17730T). An emended description of the genus Neptunomonas is also provided.

scholarly journals Carboxylicivirga linearis sp. nov., isolated from a sea cucumber culture pond

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3271-3275 ◽  
Author(s):  
Feng-Qing Wang ◽  
Yan-Xia Zhou ◽  
Xue-Zheng Lin ◽  
Guan-Jun Chen ◽  
Zong-Jun Du
Keyword(s):  
Sea Cucumber ◽  
Novel Species ◽  
Optimal Growth ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Characteristics ◽  
Respiratory Quinone ◽  
Emended Description

A yellow-pigmented, Gram-stain-negative and facultatively anaerobic bacterium, designated FB218T, was isolated from a sediment sample collected from a sea cucumber culture pond in Rongcheng, China (36° 54′ 36″ N 122° 14′ 34″ E). Cells of strain FB218T were slender, gliding, catalase-positive and oxidase-negative. Optimal growth occurred at 30 °C, pH 6.5–7.0 and in medium containing 2–3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain FB218T belonged to the genus Carboxylicivirga, family Marinilabiliaceae. The predominant fatty acids were iso-C15 : 0 and anteiso-C15 : 0. MK-7 was the main respiratory quinone. The major polar lipids of strain FB218T were two unidentified lipids and a phospholipid. The genomic DNA G+C content was 40.0 mol%. Based on the distinct phylogenetic position and the combination of physiological and phenotypic characteristics, strain FB218T represents a novel species of the genus Carboxylicivirga, for which the name Carboxylicivirga linearis sp. nov. is proposed. The type strain is FB218T ( = KCTC 42254T = MCCC 1H00106T). An emended description of the genus Carboxylicivirga is also provided.

scholarly journals Characterization and Description of Anaeromyxobacter dehalogenans gen. nov., sp. nov., an Aryl-Halorespiring Facultative Anaerobic Myxobacterium

2002 ◽  
Vol 68 (2) ◽  
pp. 893-900 ◽  
Author(s):  
Robert A. Sanford ◽  
James R. Cole ◽  
James M. Tiedje
Keyword(s):  
Electron Acceptor ◽  
Ammonium Acetate ◽  
Optimal Growth ◽  
Gliding Motility ◽  
Anaerobic Growth ◽  
16S Rdna Sequence ◽  
Rdna Sequences ◽  
Low Concentrations ◽  
Soils And Sediments ◽  
The Family

ABSTRACT Five strains were isolated which form a physiologically and phylogenetically coherent group of chlororespiring microorganisms and represent the first taxon in the Myxobacteria capable of anaerobic growth. The strains were enriched and isolated from various soils and sediments based on their ability to grow using acetate as an electron donor and 2-chlorophenol (2-CPh) as an electron acceptor. They are slender gram-negative rods with a bright red pigmentation that exhibit gliding motility and form spore-like structures. These unique chlororespiring myxobacteria also grow with 2,6-dichlorophenol, 2,5-dichlorophenol, 2-bromophenol, nitrate, fumarate, and oxygen as terminal electron acceptors, with optimal growth occurring at low concentrations (<1 mM) of electron acceptor. 2-CPh is reduced by all strains as an electron acceptor in preference to nitrate, which is reduced to ammonium. Acetate, H2, succinate, pyruvate, formate, and lactate were used as electron donors. None of the strains grew by fermentation. The 16S ribosomal DNA (rDNA) sequences of the five strains form a coherent cluster deeply branching within the family Myxococcaceae within the class Myxobacteria and are mostly closely associated with the Myxococcus subgroup. With the exception of anaerobic growth and lack of a characteristic fruiting body, these strains closely resemble previously characterized myxobacteria and therefore should be considered part of the Myxococcus subgroup. The anaerobic growth and 9.0% difference in 16S rDNA sequence from those of other myxobacterial genera are sufficient to place these strains in a new genus and species designated Anaeromyxobacter dehalogenans. The type strain is 2CP-1 (ATCC BAA-258).

scholarly journals Niabella tibetensis sp. nov., isolated from soil, and emended description of the genus Niabella

2011 ◽  
Vol 61 (5) ◽  
pp. 1201-1205 ◽  
Author(s):  
Jun Dai ◽  
Fan Jiang ◽  
Yang Wang ◽  
Bo Yu ◽  
Huan Qi ◽  
...  
Keyword(s):  
Novel Species ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
The Family ◽  
Emended Description ◽  
Taxonomic Approach ◽  
Physiological And Biochemical ◽  
Sequence Similarities ◽  
Biochemical Features

Strain 15-4T, a Gram-stain-negative, rod-shaped, non-motile bacterial strain that produced flexirubin-type pigments, was isolated from Tibet Province, China, and characterized by using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the family Chitinophagaceae, phylum ‘Bacteroidetes’, and was related to members of the genus Niabella, with sequence similarities ranging from 94.1 to 96.4 %. Strain 15-4T contained MK-7 as the predominant menaquinone and its DNA G+C content was 46.9 mol%. The major fatty acids of strain 15-4T were iso-C15 : 0 (41.3 %), iso-C15 : 1 G (14.9 %), iso-C17 : 0 3-OH (13.2 %) and summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH, 16.0 %). These chemotaxonomic data supported the affiliation of strain 15-4T to the genus Niabella. However, a number of physiological and biochemical features enabled the isolate to be differentiated phenotypically from recognized species of the genus Niabella. On the basis of the evidence presented, it is proposed that strain 15-4T represents a novel species, Niabella tibetensis sp. nov.; the type strain is 15-4T ( = CCTCC AB 209167T = NRRL B-59394T). On the basis of these data, an emended description of the genus Niabella is also proposed.

Herbiconiux moechotypicola sp. nov., a xylanolytic bacterium isolated from the gut of hairy long-horned toad beetles, Moechotypa diphysis (Pascoe)

2012 ◽  
Vol 62 (1) ◽  
pp. 90-95 ◽  
Author(s):  
Byung-Chun Kim ◽  
Doo-Sang Park ◽  
Hyangmi Kim ◽  
Hyun-Woo Oh ◽  
Kang Hyun Lee ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
Culturable Bacteria ◽  
16S Rrna Gene Sequences ◽  
Cellular Fatty Acids ◽  
The Family ◽  
Diamino Acid ◽  
Isoprenoid Quinones

A novel Gram-positive, non-motile, rod-shaped bacterium, designated strain RB-62T, was isolated during a study of culturable bacteria from the gut of Moechotypa diphysis (Pascoe) and its taxonomic position was investigated. Strain RB-62T grew at 15–30 °C and pH 5.0–8.5. The isoprenoid quinones were menaquinones MK-11 (77.1 %), MK-10 (11.7 %) and MK-12 (11.2 %). The major cellular fatty acids were anteiso-C15 : 0 (34.6 %), anteiso-C17 : 0 (29.8 %), iso-C16 : 0 (17.0 %) and cyclohexyl-C17 : 0 (11.4 %). The diagnostic diamino acid of the cell-wall peptidoglycan was 2,4-diaminobutyric acid. The G+C content of the genomic DNA of strain RB-62T was 70.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RB-62T was affiliated with the genus Herbiconiux cluster within the family Microbacteriaceae, and was related most closely to Herbiconiux ginsengi wged11T (98.08 % similarity). The level of DNA–DNA relatedness between strain RB-62T and H. ginsengi wged11T was 43.2 % (reciprocal 66.7 %). Phenotypic and phylogenetic characteristics clearly distinguished strain RB-62T from recognized species of the genus Herbiconiux. Based on data from the present polyphasic study, strain RB-62T is considered to represent a novel species of the genus Herbiconiux, for which the name Herbiconiux moechotypicola sp. nov. is proposed. The type strain is RB-62T ( = KCTC 19653T = JCM 16117T).

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