scholarly journals Rosistilla oblonga gen. nov., sp. nov. and Rosistilla carotiformis sp. nov., isolated from biotic or abiotic surfaces in Northern Germany, Mallorca, Spain and California, USA

2020 ◽  
Vol 113 (12) ◽  
pp. 1939-1952 ◽  
Author(s):  
Muhammad Waqqas ◽  
Markus Salbreiter ◽  
Nicolai Kallscheuer ◽  
Mareike Jogler ◽  
Sandra Wiegand ◽  
...  
Keyword(s):  
Cell Biology ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Optimal Growth ◽  
The Novel ◽  
Biological Features ◽  
Abiotic Surfaces ◽  
The Family ◽  
Axenic Cultures ◽  
The Baltic

AbstractPlanctomycetes are ubiquitous bacteria with fascinating cell biological features. Strains available as axenic cultures in most cases have been isolated from aquatic environments and serve as a basis to study planctomycetal cell biology and interactions in further detail. As a contribution to the current collection of axenic cultures, here we characterise three closely related strains, Poly24T, CA51T and Mal33, which were isolated from the Baltic Sea, the Pacific Ocean and the Mediterranean Sea, respectively. The strains display cell biological features typical for related Planctomycetes, such as division by polar budding, presence of crateriform structures and formation of rosettes. Optimal growth was observed at temperatures of 30–33 °C and at pH 7.5, which led to maximal growth rates of 0.065–0.079 h−1, corresponding to generation times of 9–11 h. The genomes of the novel isolates have a size of 7.3–7.5 Mb and a G + C content of 57.7–58.2%. Phylogenetic analyses place the strains in the family Pirellulaceae and suggest that Roseimaritima ulvae and Roseimaritima sediminicola are the current closest relatives. Analysis of five different phylogenetic markers, however, supports the delineation of the strains from members of the genus Roseimaritima and other characterised genera in the family. Supported by morphological and physiological differences, we conclude that the strains belong to the novel genus Rosistilla gen. nov. and constitute two novel species, for which we propose the names Rosistilla carotiformis sp. nov. and Rosistilla oblonga sp. nov. (the type species). The two novel species are represented by the type strains Poly24T (= DSM 102938T = VKM B-3434T = LMG 31347T = CECT 9848T) and CA51T (= DSM 104080T = LMG 29702T), respectively.

scholarly journals Stieleria varia sp. nov., isolated from wood particles in the Baltic Sea, constitutes a novel species in the family Pirellulaceae within the phylum Planctomycetes

2020 ◽  
Vol 113 (12) ◽  
pp. 1953-1963
Author(s):  
Frank Surup ◽  
Sandra Wiegand ◽  
Christian Boedeker ◽  
Anja Heuer ◽  
Stijn H. Peeters ◽  
...  
Keyword(s):  
Baltic Sea ◽  
Cell Biology ◽  
Novel Species ◽  
Optimal Growth ◽  
The Baltic Sea ◽  
Abiotic Surfaces ◽  
The Family ◽  
Wood Particles ◽  
Orange Colour ◽  
The Baltic

AbstractSpecies belonging to the bacterial phylum Planctomycetes are ubiquitous members of the microbial communities in aquatic environments and are frequently isolated from various biotic and abiotic surfaces in marine and limnic water bodies. Planctomycetes have large genomes of up to 12.4 Mb, follow complex lifestyles and display an uncommon cell biology; features which motivate the investigation of members of this phylum in greater detail. As a contribution to the current collection of axenic cultures of Planctomycetes, we here describe strain Pla52T isolated from wood particles in the Baltic Sea. Phylogenetic analysis places the strain in the family Pirellulaceae and suggests two species of the recently described genus Stieleria as current closest neighbours. Strain Pla52nT shows typical features of members of the class Planctomycetia, including division by polar budding and the presence of crateriform structures. Colonies of strain Pla52nT have a light orange colour, which is an unusual pigmentation compared to the majority of members in the phylum, which show either a pink to red pigmentation or entirely lack pigmentation. Optimal growth of strain Pla52nT at 33 °C and pH 7.5 indicates a mesophilic (i.e. with optimal growth between 20 and 45 °C) and neutrophilic growth profile. The strain is an aerobic heterotroph with motile daughter cells. Its genome has a size of 9.6 Mb and a G + C content of 56.0%. Polyphasic analyses justify delineation of the strain from described species within the genus Stieleria. Therefore, we conclude that strain Pla52nT = LMG 29463T = VKM B-3447T should be classified as the type strain of a novel species, for which we propose the name Stieleria varia sp. nov.

scholarly journals Three Planctomycetes isolated from biotic surfaces in the Mediterranean Sea and the Pacific Ocean constitute the novel species Symmachiella dynata gen. nov., sp. nov. and Symmachiella macrocystis sp. nov.

2020 ◽  
Vol 113 (12) ◽  
pp. 1965-1977
Author(s):  
Markus Salbreiter ◽  
Muhammad Waqqas ◽  
Mareike Jogler ◽  
Nicolai Kallscheuer ◽  
Sandra Wiegand ◽  
...  
Keyword(s):  
Cell Biology ◽  
Novel Species ◽  
Optimal Growth ◽  
Monterey Bay ◽  
The Novel ◽  
Separate Species ◽  
The Pacific ◽  
The Family ◽  
The Pacific Ocean ◽  
Significant Attention

AbstractPlanctomycetes is a phylum of environmentally important bacteria, which also receive significant attention due to their fascinating cell biology. Access to axenic Planctomycete cultures is crucial to study cell biological features within this phylum in further detail. In this study, we characterise three novel strains, Mal52T, Pan258 and CA54T, which were isolated close to the coasts of the islands Mallorca (Spain) and Panarea (Italy), and from Monterey Bay, CA, USA. The three isolates show optimal growth at temperatures between 22 and 24 °C and at pH 7.5, divide by polar budding, lack pigmentation and form strong aggregates in liquid culture. Analysis of five phylogenetic markers suggests that the strains constitute two novel species within a novel genus in the family Planctomycetaceae. The strains Mal52T (DSM 101177T = VKM B-3432T) and Pan258 were assigned to the species Symmachiella dynata gen nov., sp. nov., while strain CA54T (DSM 104301T = VKM B-3450T) forms a separate species of the same genus, for which we propose the name Symmachiella macrocystis sp. nov.

scholarly journals Pelagicoccus croceus sp. nov., a novel marine member of the family Puniceicoccaceae within the phylum ‘Verrucomicrobia’ isolated from seagrass

2007 ◽  
Vol 57 (12) ◽  
pp. 2874-2880 ◽  
Author(s):  
Jaewoo Yoon ◽  
Naoya Oku ◽  
Satoru Matsuda ◽  
Hiroaki Kasai ◽  
Akira Yokota
Keyword(s):  
Cell Wall ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
The Novel ◽  
Antibiotic Susceptibility Test ◽  
The Family ◽  
Lactam Antibiotic

An obligately aerobic, spherical, non-motile, pale-yellow pigmented bacterium was isolated from a piece of leaf of seagrass, Enhalus acoroides (L.f.) Royle, grown in Okinawa, Japan and was subjected to a polyphasic taxonomic study. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the novel isolate N5FB36-5T shared approximately 96–98 % sequence similarity with the species of the genus Pelagicoccus of the family Puniceicoccaceae within the phylum ‘Verrucomicrobia’. The DNA–DNA relatedness values of strain N5FB36-5T with Pelagicoccus mobilis 02PA-Ca-133T and Pelagicoccus albus YM14-201T were below 70 %, which is accepted as the phylogenetic definition of a novel species. β-Lactam antibiotic susceptibility test and amino acid analysis of the cell wall hydrolysates indicated the absence of muramic acid and diaminopimelic acid in the cell walls, which suggested that this strain lacks an ordinary Gram-negative type of peptidoglycan in the cell wall. The DNA G+C content of strain N5FB36-5T was 51.6 mol%; MK-7 was the major menaquinone; and the presence of C16 : 0, C16 : 1 ω7c and anteiso-C15 : 0 as the major cellular fatty acids supported the identification of the novel isolate as a member of the genus Pelagicoccus. On the basis of polyphasic taxonomic data, it was concluded that this strain should be classified as a novel species of the genus Pelagicoccus, for which the name Pelagicoccus croceus sp. nov. is proposed. The type strain is N5FB36-5T (=MBIC08282T=KCTC 12903T).

scholarly journals Updates to the recently introduced family Lacipirellulaceae in the phylum Planctomycetes: isolation of strains belonging to the novel genera Aeoliella, Botrimarina, Pirellulimonas and Pseudobythopirellula and the novel species Bythopirellula polymerisocia and Posidoniimonas corsicana

2020 ◽  
Vol 113 (12) ◽  
pp. 1979-1997
Author(s):  
Sandra Wiegand ◽  
Mareike Jogler ◽  
Christian Boedeker ◽  
Anja Heuer ◽  
Stijn H. Peeters ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
The Novel ◽  
Generation Times ◽  
The Family ◽  
Resource Collection ◽  
The Baltic ◽  
Mallorca Island ◽  
Costa Brava ◽  
The Phylum Planctomycetes

AbstractEight novel strains of the phylum Planctomycetes were isolated from different aquatic habitats. Among these habitats were the hydrothermal vent system close to Panarea Island, a public beach at Mallorca Island, the shore of Costa Brava (Spain), and three sites with brackish water in the Baltic Sea. The genome sizes of the novel strains range from 4.33 to 6.29 Mb with DNA G+C contents between 52.8 and 66.7%. All strains are mesophilic (Topt 24–30 °C) and display generation times between 17 and 94 h. All eight isolates constitute novel species of either already described or novel genera within the family Lacipirellulaceae. Two of the novel species, Posidoniimonas polymericola (type strain Pla123aT = DSM 103020T = LMG 29466T) and Bythopirellula polymerisocia (type strain Pla144T = DSM 104841T = VKM B-3442T), belong to established genera, while the other strains represent the novel genera Aeoliella gen. nov., Botrimarina gen. nov., Pirellulimonas gen. nov. and Pseudobythopirellula gen. nov. Based on our polyphasic analysis, we propose the species Aeoliella mucimassa sp. nov. (type strain Pan181T = DSM 29370T = LMG 31346T = CECT 9840T = VKM B-3426T), Botrimarina colliarenosi sp. nov. (type strain Pla108T = DSM 103355T = LMG 29803T), Botrimarina hoheduenensis sp. nov. (type strain Pla111T = DSM 103485T = STH00945T, Jena Microbial Resource Collection JMRC), Botrimarina mediterranea sp. nov. (type strain Spa11T = DSM 100745T = LMG 31350T = CECT 9852T = VKM B-3431T), Pirellulimonas nuda sp. nov. (type strain Pla175T = DSM 109594T = CECT 9871T = VKM B-3448T) and Pseudobythopirellula maris sp. nov. (type strain Mal64T = DSM 100832T = LMG 29020T).

Boeremia parva sp. nov., a novel species of the family Didymellaceae isolated from soil

Phytotaxa ◽  
2021 ◽  
Vol 518 (1) ◽  
pp. 25-35
Author(s):  
KALLOL DAS ◽  
LEONID N. TEN ◽  
JAE-HO BAN ◽  
SEUNG-YEOL LEE ◽  
HEE-YOUNG JUNG
Keyword(s):  
Dna Sequences ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Phylogenetic Position ◽  
The Novel ◽  
Closely Related Species ◽  
The Family ◽  
Colony Color ◽  
Average Size ◽  
Isolated Species

Fungal strains, designated KNU-NL4 and KNU-OL2, belonging to the family Didymellaceae were isolated from a soil sample collected in Miryang, Korea. Phylogenetic analyses based on a concatenated dataset of DNA sequences of ITS regions and partial sequences of ACT, CAL, TEF1-α, and β-TUB genes showed that the isolates reside in a clade together with Boeremia species but occupy the distinct phylogenetic position. Morphologically, the novel strains produce bigger conidiomata (average size 169.8 μm) than the closely related B. rhapontica (126.59 μm) and smaller than the other close neighbor B. coffeae (187.5 μm). Both novel strains also differed from them by smaller colony size and colony color on OA and MEA. The detailed descriptions, illustrations, and discussions regarding the morphological and phylogenetic analyses of the closely related species are provided to support the novelty of the isolated species. The results of phylogenetic analysis and morphological observations indicate that strains KNU-NL4 and KNU-OL2 represent a novel species in the genus Boeremia, for which the name Boeremia parva sp. nov. is proposed.

scholarly journals Thalassomonas agarivorans sp. nov., a marine agarolytic bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the genus Thalassomonas

2006 ◽  
Vol 56 (6) ◽  
pp. 1245-1250 ◽  
Author(s):  
Wen Dar Jean ◽  
Wung Yang Shieh ◽  
Tung Yen Liu
Keyword(s):  
Novel Species ◽  
Optimal Growth ◽  
Anaerobic Growth ◽  
The Novel ◽  
Cellular Fatty Acids ◽  
Water Region ◽  
The Family ◽  
Emended Description ◽  
Curved Rods ◽  
Shallow Water Region

A marine agarolytic bacterium, designated strain TMA1T, was isolated from a seawater sample collected in a shallow-water region of An-Ping Harbour, Taiwan. It was non-fermentative and Gram-negative. Cells grown in broth cultures were straight or curved rods, non-motile and non-flagellated. The isolate required NaCl for growth and exhibited optimal growth at 25 °C and 3 % NaCl. It grew aerobically and was incapable of anaerobic growth by fermenting glucose or other carbohydrates. Predominant cellular fatty acids were C16 : 0 (17.5 %), C17 : 1 ω8c (12.8 %), C17 : 0 (11.1 %), C15 : 0 iso 2-OH/C16 : 1 ω7c (8.6 %) and C13 : 0 (7.3 %). The DNA G+C content was 41.0 mol%. Phylogenetic, phenotypic and chemotaxonomic data accumulated in this study revealed that the isolate could be classified in a novel species of the genus Thalassomonas in the family Colwelliaceae. The name Thalassomonas agarivorans sp. nov. is proposed for the novel species, with TMA1T (=BCRC 17492T=JCM 13379T) as the type strain.

scholarly journals Description of Polystyrenella longa gen. nov., sp. nov., isolated from polystyrene particles incubated in the Baltic Sea

2020 ◽  
Vol 113 (12) ◽  
pp. 1851-1862 ◽  
Author(s):  
Stijn H. Peeters ◽  
Sandra Wiegand ◽  
Nicolai Kallscheuer ◽  
Mareike Jogler ◽  
Anja Heuer ◽  
...  
Keyword(s):  
Cell Division ◽  
Baltic Sea ◽  
Cell Biology ◽  
Aquatic Ecosystems ◽  
Novel Species ◽  
The Baltic Sea ◽  
Motile Cells ◽  
The Family ◽  
The Baltic ◽  
Almost All

AbstractPlanctomycetes occur in almost all aquatic ecosystems on earth. They have a remarkable cell biology, and members of the orders Planctomycetales and Pirellulales feature cell division by polar budding, perform a lifestyle switch from sessile to motile cells and have an enlarged periplasmic space. Here, we characterise a novel planctomycetal strain, Pla110T, isolated from the surface of polystyrene particles incubated in the Baltic Sea. After phylogenetic analysis, the strain could be placed in the family Planctomycetaceae. Strain Pla110T performs cell division by budding, has crateriform structures and grows in aggregates or rosettes. The strain is a chemoheterotroph, grows under mesophilic and neutrophilic conditions, and exhibited a doubling time of 21 h. Based on our phylogenetic and morphological characterisation, strain Pla110T (DSM 103387T = LMG 29693T) is concluded to represent a novel species belonging to a novel genus, for which we propose the name Polystyrenella longa gen. nov., sp. nov.

scholarly journals Cryolevonia gen. nov. and Cryolevonia schafbergensis sp. nov., a cryophilic yeast from ancient permafrost and melted sea ice

2020 ◽  
Vol 70 (4) ◽  
pp. 2334-2338 ◽  
Author(s):  
Ana Pontes ◽  
Joel Ruethi ◽  
Beat Frey ◽  
Andreia Aires ◽  
Amanda Thomas ◽  
...  
Keyword(s):  
Sea Ice ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Its Region ◽  
Swiss Alps ◽  
Sexual Cycle ◽  
The Novel ◽  
Prolonged Incubation ◽  
D2 Domain ◽  
The Family

A cryophilic basidiomycetous yeast unable to grow at 18 °C or higher temperatures was isolated from a subsurface permafrost layer collected in the Eastern Swiss Alps and from melted sea ice collected in the Artic at Frobisher Bay, Nunavut, Canada. Phylogenetic analyses employing combined sequences of the D1/D2 domain and ITS region indicated that the two new isolates belong to the family Camptobasidiaceae of the class Microbotryomycetes but are distantly related to any of the currently recognized species and genera. Consequently, the novel genus Cryolevonia, and the novel species Cryolevonia schafbergensis (type strain PYCC 8347T=CBS 16055T) are proposed to accommodate this cryophilic yeast. Although sparse hyphae and teliospore-like stuctures were observed upon prolonged incubation, a sexual cycle was not observed and therefore C. schafbergensis is documented solely from its asexual stage.

scholarly journals Multiple Types of Novel Enteric Bopiviruses (Picornaviridae) with the Possibility of Interspecies Transmission Identified from Cloven-Hoofed Domestic Livestock (Ovine, Caprine and Bovine) in Hungary

Viruses ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 66
Author(s):  
Zoltán László ◽  
Péter Pankovics ◽  
Gábor Reuter ◽  
Attila Cságola ◽  
Ádám Bálint ◽  
...  
Keyword(s):  
Novel Species ◽  
Phylogenetic Analyses ◽  
Interspecies Transmission ◽  
Background Information ◽  
Type Ii ◽  
Rt Pcr ◽  
Faecal Samples ◽  
The Family ◽  
Domestic Livestock

Most picornaviruses of the family Picornaviridae are relatively well known, but there are certain “neglected” genera like Bopivirus, containing a single uncharacterised sequence (bopivirus A1, KM589358) with very limited background information. In this study, three novel picornaviruses provisionally called ovipi-, gopi- and bopivirus/Hun (MW298057-MW298059) from enteric samples of asymptomatic ovine, caprine and bovine respectively, were determined using RT-PCR and dye-terminator sequencing techniques. These monophyletic viruses share the same type II-like IRES, NPGP-type 2A, similar genome layout (4-3-4) and cre-localisations. Culture attempts of the study viruses, using six different cell lines, yielded no evidence of viral growth in vitro. Genomic and phylogenetic analyses show that bopivirus/Hun of bovine belongs to the species Bopivirus A, while the closely related ovine-origin ovipi- and caprine-origin gopivirus could belong to a novel species “Bopivirus B” in the genus Bopivirus. Epidemiological investigation of N = 269 faecal samples of livestock (ovine, caprine, bovine, swine and rabbit) from different farms in Hungary showed that bopiviruses were most prevalent among <12-month-old ovine, caprine and bovine, but undetectable in swine and rabbit. VP1 capsid-based phylogenetic analyses revealed the presence of multiple lineages/genotypes, including closely related ovine/caprine strains, suggesting the possibility of ovine–caprine interspecies transmission of certain bopiviruses.

Lecanicillium subprimulinum (Cordycipitaceae, Hypocreales), a novel species from Baoshan, Yunnan

Phytotaxa ◽  
2018 ◽  
Vol 348 (2) ◽  
pp. 99 ◽  
Author(s):  
SHIKE HUANG ◽  
SAJEEWA S.N. MAHARACHCHIKUMBURA ◽  
RAJESH JEEWON ◽  
D JAYARAMA BHAT ◽  
RUNGTIWA PHOOKAMSAK ◽  
...  
Keyword(s):  
New Species ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Its Sequence ◽  
New Taxon ◽  
Sexual Morph ◽  
The Family ◽  
Asexual Morphs ◽  
A New Species

In this paper, we report a new asexual-morph taxon belonging to the family Cordycipitaceae (Hypocreales), which is associated with a sexual morph of an ophioceras-like taxon on submerged wood collected from Baoshan, Yunnan, China. Morphologically, this new taxon is similar to known asexual morphs of Lecanicillium and characterized by conidiophores arising from hyaline hyphae, with gregarious, ellipsoid to ovoid, aseptate conidia. Phylogenetic analyses of a combined LSU, SSU, tef1-α and ITS sequence dataset positions our taxon in Cordycipitaceae and close to Lecanicillium sp. (CBS 639.85) and L. primulinum. Lecanicillium subprimulinum is introduced as a new species with support from molecular data.

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