scholarly journals Analysis of ACE, AGTR1, eNOS, MTHFR, MTRR and APOE genes polymorphisms in the population of North-West of Russia

2004 ◽  
Vol 2 (3) ◽  
pp. 32-35
Author(s):  
Oleg S Glotov ◽  
Andrey S Glotov ◽  
Olga A Tarasenko ◽  
Tatyana E Ivashchenko ◽  
Vladislav S Baranov
Keyword(s):  
High Frequency ◽  
Middle Age ◽  
Low Frequency ◽  
Rflp Analysis ◽  
The Other ◽  
North West ◽  
Multifactorial Diseases ◽  
Pcr Rflp ◽  
Obvious Similarity ◽  
European Populations

Estimation of population-specific frequencies of relevant polymorphisms in normal control population remains the major prerequisite of these studies. The polymorphisms of seven genes (ACE, AGTR1, eNOS, MTHFR, MTRR, APOE) associated with some multifactorial diseases (cardiovascular diseases, diabetes) were studied by PCR-RFLP analysis in North-West population of Russia. The group consisted of unrelated individuals of middle age (25-45) (59 men and 58 women). Distribution of relevant polymorphisms frequencies for MTHFR, MTRR, APOE, eNOS and AGTR1 genes was similar in our samples compared to these ones in some and European populations (Spain and Germany). Low frequency of D/D genotype and high frequency of I/I genotype were found in Russian compared to Spain population (D/D - 29,6 and 41%, I/I - 26,1 and 15%, respectively). No significant statistical differences in distribution of ACE polymorphisms were found between Russian and Scotland populations (D/D - 31%, I/I - 20%). This population specific differences for the polymorphisms of some genes and its obvious similarity for the other ones were disclosed

Genetic polymorphisms of cytochrome P450 enzymes: CYP2C9, CYP2C19, CYP2D6, CYP3A4, and CYP3A5 in the Croatian population

2017 ◽  
Vol 32 (1) ◽  
Author(s):  
Lana Ganoci ◽  
Tamara Božina ◽  
Nikica Mirošević Skvrce ◽  
Mila Lovrić ◽  
Petar Mas ◽  
...  
Keyword(s):  
Rflp Analysis ◽  
The Other ◽  
Published Data ◽  
Cytochrome P450 Enzymes ◽  
Chain Reaction ◽  
Allelic Variants ◽  
Mediterranean Populations ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
European Populations

AbstractBackground:Data on the frequency of pharmacogenetic polymorphisms in the Croatian population are limited. We determined and analyzed frequencies for the most importantMethods:2637 subjects were included. Genotyping was performed by real-time polymerase chain reaction (PCR) using TaqMan® DME or TaqMan® SNP Genotyping Assays, and by PCR, and PCR-RFLP analysis.Results:ForConclusions:The frequency of the CYP allelic variants, genotypes, and predicted phenotypes in the Croatian population is in accordance with the other European populations, between the values of published data for Middle European and Mediterranean populations.

Ion-cyclotron modes in weakly relativistic plasmas

1994 ◽  
Vol 51 (3) ◽  
pp. 371-379 ◽  
Author(s):  
Chandu Venugopal ◽  
P. J. Kurian ◽  
G. Renuka
Keyword(s):  
Dispersion Relation ◽  
High Frequency ◽  
Low Frequency ◽  
Dispersion Relations ◽  
The Other ◽  
Larmor Radius ◽  
Relativistic Plasmas ◽  
Ion Plasma ◽  
Maxwellian Plasma ◽  
Relativistic Dispersion

We derive a dispersion relation for the perpendicular propagation of ioncyclotron waves around the ion gyrofrequency ω+ in a weaklu relaticistic anisotropic Maxwellian plasma. These waves, with wavelength greater than the ion Larmor radius rL+ (k⊥ rL+ < 1), propagate in a plasma characterized by large ion plasma frequencies (). Using an ordering parameter ε, we separated out two dispersion relations, one of which is independent of the relativistic terms, while the other depends sensitively on them. The solutions of the former dispersion relation yield two modes: a low-frequency (LF) mode with a frequency ω < ω+ and a high-frequency (HF) mode with ω > ω+. The plasma is stable to the propagation of these modes. The latter dispersion relation yields a new LF mode in addition to the modes supported by the non-relativistic dispersion relation. The two LF modes can coalesce to make the plasma unstable. These results are also verified numerically using a standard root solver.

scholarly journals Characterisation of avian Pasteurella multocida strains with PCR-RFLP analysis of the ompH gene

2013 ◽  
Vol 61 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Boglárka Sellyei ◽  
Éva Ivanics ◽  
Tibor Magyar
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Pasteurella Multocida ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
The Other ◽  
Pcr Rflp ◽  
H Gene ◽  
Geographic Regions ◽  
Type Strains ◽  
Restriction Fragment Length

The 16 somatic serotype type strains and 60 field isolates of Pasteurella multocida, representing various avian species and geographic regions in Hungary, were characterised by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the ompH gene with DraI restriction endonuclease. The type strains yielded eight different (I-VIII) profiles. Strains whose PCR fragment was uncut by DraI (profile IV) could be differentiated with HindIII and PvuII restriction endonucleases. Five of the eight PCR-RFLP profiles (I, III, V, VI and VII) were detected among the field strains. Only a correlation of limited strength was found between the classical somatic serotypes and the PCR-RFLP profiles. However, the results confirmed that molecular methods could confidently distinguish serotype A:1 strains from the other serotypes. Moreover, the specific relationship between somatic serotypes and PCR-RFLP types among isolates from turkey raises the possibility of the existence of host-specific clones within the P. multocida population.

P3392Potential of imaging-guided PCI for event suppression in Japanese acute myocardial infarction patients: J-MINUET substudy

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
T Yamashita ◽  
K Sakamoto ◽  
K Tsujita ◽  
K Nakao ◽  
Y Ozaki ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
High Frequency ◽  
Low Frequency ◽  
The Other ◽  
Intravascular Imaging ◽  
Clinical Events ◽  
Coronary Imaging ◽  
Usage Frequency ◽  
Moderate Frequency ◽  
Event Rates

Abstract Background Intravascular ultrasound (IVUS) and Optical Coherence Tomography (OCT) has been widely used in clinical settings. Although favorable results of imaging-guided percutaneous coronary intervention (PCI) compared with angio-guided PCI were observed in several studies, impacts of institutional-based usage frequency, about imaging-guided PCI, have not been well elucidated. Methods To elucidate the impact of imaging-guided PCI and the effects of frequency of its usage, we analyzed data of the Japanese registry of acute Myocardial INfarction diagnosed by Universal dEfiniTion (J-MINUET). This was a prospective and multicenter registry consisting of 3,283 AMI patients, who were hospitalized within 48 hours of onset from July 2012 to March 2014. Clinical follow-up data was obtained for 3 years. In this sub-study, a total of 2,788 patients who underwent urgent PCI having detailed procedural information were enrolled. We analyzed the differences of utilization rates of imaging-guided PCI among the participating institutions and the impacts for the clinical events. The participating institutions were divided into 3 groups by the frequency of IVUS usage: low frequency institutions: under 50%; moderate frequency institutions: 50% to 90%; and, high frequency institutions: over 90%. Results In this cohort registry, patients were enrolled from 28 institutions. The utilization rate of coronary imaging varied widely depending on each institution from 15.4% to 100% (mean 85.7%±24.3, median 97.4%). When the institutions were divided into 3 groups by the frequency of intravascular imaging usage, four low frequency institutions enrolled 295 patients, five moderate frequency institutions enrolled 624 patients, and 19 high frequency institutions enrolled 1,491 patients. Although the incidence of MACE (death, MI, stroke, cardiac failure, or revascularization for unstable angina) decreased stepwise (33.2%, 23.7%, and 19.7%) (gray bar in the Figure), the event rates of the imaging-guided PCI cases among the 3 groups were comparable (21.6%, 21.9%, and 19.6%) (white bar in the Figure). On the other hand, a gradual event reduction between the 3 groups was observed in the angio-guided PCI cases (black bar in the Figure). In comparison of MACE rate between imaging-guided and angio-guided PCI, there were statistically significant differences in the low frequency and moderate frequency institutions (p=0.001 and p=0.012, respectively). In contrast, comparable event rates were observed in the high frequency institutions (p=0.441). MACE rate by imaging usage frequency Conclusions In Japanese ACS patients treated with imaging-guided PCI, better suppression of clinical events during 3-year was found in the institutions with the more frequent use of intravascular imaging, mainly due to stepwise event suppression in the cases of angio-guided PCI. On the other hand, the clinical benefit of coronary imaging was obtained independently of the frequency of use and its experience.

scholarly journals Optimizing smoke alarm signals: Testing the effectiveness of children’s smoke alarms for sleeping adults

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Gary A. Smith ◽  
Sandhya Kistamgari ◽  
Mark Splaingard
Keyword(s):  
Median Time ◽  
High Frequency ◽  
Low Frequency ◽  
The Other ◽  
Wilcoxon Test ◽  
Smoke Alarm ◽  
Smoke Alarms ◽  
Alarm Signals ◽  
Frequency Tone ◽  
Residential Fire

Abstract Background Being asleep is an important risk factor for death during a residential fire; however, the high-frequency tone smoke alarms in many homes will not adequately awaken children who are old enough to self-rescue. In a series of previous studies, we identified smoke alarm signals that effectively awaken children 5–12 years old and prompt their escape. Because it is impractical to have separate alarms for children and adults in a household, the purpose of this study is to test whether alarms that are effective in awakening children and prompting their escape are also effective among adults. Methods Using a randomized, non-blinded, repeated measures design, 150 adults 20–49 years old were exposed during stage 4 sleep to four different smoke alarms. Statistical tests included the Kaplan-Meier estimator, generalized Wilcoxon test, and hazard ratios with Wald’s 95% confidence intervals. Results The median age of study subjects was 30.0 years and 67.3% were female. Almost all (n = 149) subjects awakened and performed the escape procedure to all four alarms; one individual did not awaken or escape to the high-frequency tone alarm. The median time-to-awaken was 2.0 s for the high-frequency tone alarm and 1.0 s for the other three alarms. The median time-to-escape for the high-frequency tone alarm was 12.0 s, compared with 10.0 s for the low-frequency tone alarm and 9.0 s each for the female and male voice alarms. All pairwise comparisons between the high-frequency tone alarm and each of the other three alarms were statistically significant for the probability functions for time-to-awaken and time-to-escape. There were no significant differences in these outcome measures between the latter three alarms, except for female voice versus low-frequency tone alarms for time-to-escape. Conclusions All alarms performed well, demonstrating that smoke alarms developed for the unique developmental requirements of sleeping children are also effective among sleeping adults. Compared with a high-frequency tone alarm, use of these alarms may reduce residential fire-related injuries and deaths among children, while also successfully alerting adult members of the household.

scholarly journals THE AGRO-PASTORAL CONFLICTS AND FORAGE SPECIES PRIZED BY CATTLE: CASE OF THE NORTHERN PERIPHERY OF THE MBAM AND DJEREM NATIONAL PARK (ADAMAOUA-CAMEROON)

2021 ◽  
Vol 9 (02) ◽  
pp. 965-985
Author(s):  
Marie Mvu Njoya ◽  
◽  
Ambahe Duplex Rufin ◽  
Keyword(s):  
High Frequency ◽  
National Park ◽  
Low Frequency ◽  
Partial Solution ◽  
The Other ◽  
Survey Method ◽  
Other Hand ◽  
Forage Species ◽  
The One ◽  
The Relationship

In the outskirts of the northern periphery of Mbam and Djerem National Park, agro-pastoral conflicts have become very frequent in recent years. The resulting damage is so surprising that it leaves no one indifferent. Unfortunately, many writings analyze the causes of these agro-pastoral conflicts without, however, dwelling on the fodder resources which constitute not only the staple food of cattle but also one of the primary factors of these antagonisms. To overcome this knowledge gap, the authors adopted the survey method in order to identify, on the one hand, the attributes of agro-pastoral conflicts and the plant species consumed in all seasons by cattle on the northern periphery of the PNMD and, on the other hand, the relationship between these forage species with the frequency of agro-pastoral conflicts according to the protagonists and the seasons. Thus, from field observations and a questionnaire sent to 44 breeders and 103 farmers in four control villages, it results, after processing the data, that the high frequency of conflicts experienced by the farmers in the dry season (35.9 %) and especially by the breeders (70.5%) is due to the location of the forage species prized in this season at the bottom of the valleys where the herds are concentrated and compete. On the other hand, the low frequency of conflicts experienced during the rainy season especially by breeders, except in Mbitom, is more justified by the extension of fodder registers to woody leaves from the start of this season. Finally, the fodder fields of Brachiaria constitute a partial solution to the shortage of pastures even if it sometimes gives rise to new conflicts between herders.

scholarly journals Estrus Behaviour and Reproductive Traits of Pulawska Gilts Associated with Selected Gene Polymorphisms

2021 ◽  
Vol 21 (1) ◽  
pp. 159-172
Author(s):  
Marek Babicz ◽  
Marcin Pastwa ◽  
Magdalena Szyndler-Nędza ◽  
Anna Kozubska-Sobocińska ◽  
Barbara Danielak-Czech ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Gene Polymorphisms ◽  
Rflp Analysis ◽  
Reproductive Traits ◽  
The Other ◽  
Molecular Analyses ◽  
Native Breed ◽  
Hair Roots ◽  
Pcr Rflp ◽  
Pcr Conditions

Abstract Searching for the associations between the gene polymorphism and the reproductive traits is essential in defining the genetic native breed specificity, which distinguishes them from the other breeds. The aim of our study was to determine the associations between mutations in the PRL, PRLR, PTGS2, FUT1 genes and sexual and periparturient activity in native Pulawska gilts. The analysis included 72 animals which gave birth to the first litter. Evaluation of the productive value of gilts accounted for indicators of sexual and periparturient activity as well as reproductive traits. The biological material for molecular analyses was obtained from the hair roots of the gilts. The genotype was verified by PCR RFLP analysis. The primers and PCR conditions were determined on the basis of available literature data. Statistically significant differences (P≤0.05) were found at the PRL locus: gilts of AA genotypes (Ins/Ins) at the PRL locus were characterised by longest farrowing duration compared to gilts of AB genotype (P≤0.05). The analysis of PRLR gene showed that gilts of TT genotype revealed a tendency for later occurrence of estrus signs (first and second estrus) and for the markedly longest farrowings (P≤0.05). With regard to PTGS2 and FUT1 loci, no significant differences were found in the parameters of sexual and periparturient activity of the gilts. However, gilts of FUT1 GG genotype gave birth to and reared the largest first litters (P≤0.05). The results of the studies expand the knowledge about the genetic structure and productivity of Pulawska gilts.

Existence of Dominant Players and their Role in the Formation of a Cabinet Coalition

2017 ◽  
Vol 19 (01) ◽  
pp. 1750005
Author(s):  
Artyom Jelnov
Keyword(s):  
Empirical Evidence ◽  
High Frequency ◽  
Winning Coalition ◽  
Low Frequency ◽  
The Other ◽  
Dominant Party ◽  
Frequency Occurrence

A party is dominant if there is a majority coalition to which that party belongs such that it affords this party more possibilities to form an alternative winning coalition than any of the other members of the coalition. I present empirical evidence showing that an allocation of seats in a parliament is biased toward the high frequency occurrence of a dominant party and the low frequency occurrence of a dictator. If a dominant party forms a cabinet coalition, and if that cabinet coalition has a majority in parliament, then the dominant party tends to form a coalition which it dominates.

scholarly journals Modifikasi Permukaan Elemen Peredam Bising Dengan Profil Berbentuk Prisma

2016 ◽  
Vol 6 (01) ◽  
Author(s):  
Hanif Azimut
Keyword(s):  
High Frequency ◽  
Sound Absorption ◽  
Coupling Effect ◽  
Low Frequency ◽  
The Other ◽  
Frequency Range ◽  
Closed Type ◽  
Wave Resonance ◽  
Half Wave ◽  
Absorption Performance

<p class="AbstractText">The influence of surface modification by using prism shaped profile on the sound absorption of absorber element was investigated experimentally. A prime number based configuration of the two types opened and closed type rism shaped profile inclusion was tested by using impedance tube according ASTM E1050 standard. The result shows that sound absorption at low frequency band below 200 Hz increased with the increasing of closed prism number. It is related to the coupling effect between the cavities of the absorber element and the prisms that changes reactance of the coupled structure. In the other side, a half wave resonance like effect occur on the use of opened prisms inclusion, which is increase the sound absorption performance at mid to high frequency range between 800 Hz to 1,25 kHz.  </p>

scholarly journals Abiotrophia elegans Strains Comprise 8% of the Nutritionally Variant Streptococci Isolated from the Human Mouth

1999 ◽  
Vol 37 (8) ◽  
pp. 2553-2556 ◽  
Author(s):  
Setsuko Sato ◽  
Taisei Kanamoto ◽  
Masakazu Inoue
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Rflp Analysis ◽  
The Other ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Biochemical Tests ◽  
Pcr Rflp ◽  
Adh Activity

Ninety-one isolates of nutritionally variant streptococci (NVS) that were previously isolated from the human mouth were regarded as consisting of 7 Streptococcus defectivus isolates, 78Streptococcus adjacens isolates, and 6 Gemella morbillorum isolates. However, recent references to the taxonomic reclassification of NVS, from S. defectivusto Abiotrophia defectiva and from S. adjacensto Abiotrophia adiacens, and the newly introduced speciesAbiotrophia elegans as a third Abiotrophiaspecies, emphasize the need for genetic analyses for identification of NVS. When PCR-restriction fragment length polymorphism (RFLP) and phylogenetic distances were examined based on 16S rRNA gene sequences, the results indicated that 7 of the 91 NVS isolates were closely related to A. elegans. These seven isolates consisted of four isolates previously identified as G. morbillorum and three isolates previously identified as S. adjacens. Two isolates previously identified as G. morbillorum were related to A. adiacens. In biochemical tests, A. elegans and the seven isolates related to it possessed arginine dihydrolase (ADH) activity but the other Abiotrophia species did not. As a result, A. elegans strains comprised 8% of the 91 NVS isolates. Our findings suggest that A. elegans, A. adiacens, and A. defectiva exist in the human mouth in proportions of about 1:11:1 and that A. elegans can be genetically distinguished from the other twoAbiotrophia species by PCR-RFLP analysis of 16S rRNA gene sequences and can be biochemically distinguished by ADH activity.

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