scholarly journals High quality genome assembly of the amitochondriate eukaryote Monocercomonoides exilis

2021 ◽  
Vol 7 (12) ◽  
Author(s):  
Sebastian Cristian Treitli ◽  
Priscila Peña-Diaz ◽  
Paweł Hałakuc ◽  
Anna Karnkowska ◽  
Vladimír Hampl
Keyword(s):  
Genome Assembly ◽  
Complete Genome ◽  
Untranslated Region ◽  
Gene Prediction ◽  
Draft Genome ◽  
High Quality ◽  
Kozak Sequence ◽  
Oxford Nanopore ◽  
High Quality Genome ◽  
Polyadenylation Signals

Monocercomonoides exilis is considered the first known eukaryote to completely lack mitochondria. This conclusion is based primarily on a genomic and transcriptomic study which failed to identify any mitochondrial hallmark proteins. However, the available genome assembly has limited contiguity and around 1.5 % of the genome sequence is represented by unknown bases. To improve the contiguity, we re-sequenced the genome and transcriptome of M. exilis using Oxford Nanopore Technology (ONT). The resulting draft genome is assembled in 101 contigs with an N50 value of 1.38 Mbp, almost 20 times higher than the previously published assembly. Using a newly generated ONT transcriptome, we further improve the gene prediction and add high quality untranslated region (UTR) annotations, in which we identify two putative polyadenylation signals present in the 3′UTR regions and characterise the Kozak sequence in the 5′UTR regions. All these improvements are reflected by higher BUSCO genome completeness values. Regardless of an overall more complete genome assembly without missing bases and a better gene prediction, we still failed to identify any mitochondrial hallmark genes, thus further supporting the hypothesis on the absence of mitochondrion.

scholarly journals Chromosome-scale assembly of the yellow mealworm genome

2021 ◽  
Vol 1 ◽  
pp. 94
Author(s):  
Evangelia Eleftheriou ◽  
Jean-Marc Aury ◽  
Benoît Vacherie ◽  
Benjamin Istace ◽  
Caroline Belser ◽  
...  
Keyword(s):  
Gene Prediction ◽  
Scale Production ◽  
Rna Seq ◽  
High Quality ◽  
Yellow Mealworm ◽  
Promising Alternative ◽  
Alternative Protein ◽  
Oxford Nanopore ◽  
Mealworm Beetle ◽  
High Quality Genome

Background: The yellow mealworm beetle, Tenebrio molitor, is a promising alternative protein source for animal and human nutrition and its farming involves relatively low environmental costs. For these reasons, its industrial scale production started this century. However, to optimize and breed sustainable new T. molitor lines, the access to its genome remains essential. Methods: By combining Oxford Nanopore and Illumina Hi-C data, we constructed a high-quality chromosome-scale assembly of T. molitor. Then, we combined RNA-seq data and available coleoptera proteomes for gene prediction with GMOVE. Results: We produced a high-quality genome with a N50 = 21.9Mb with a completeness of 99.5% and predicted 21,435 genes with a median size of 1,780 bp. Gene orthology between T. molitor and Tribolium castaneaum showed a highly conserved synteny between the two coleoptera. Conclusions: The present genome will greatly help fundamental and applied research such as genetic breeding and will contribute to the sustainable production of the yellow mealworm.

scholarly journals Complete Genome Sequence of Pseudomonas sp. Strain SGAir0191, Isolated from Tropical Air Collected in Singapore

2019 ◽  
Vol 8 (34) ◽  
Author(s):  
Anthony Wong ◽  
Ana Carolina M. Junqueira ◽  
Ankur Chaturvedi ◽  
Akira Uchida ◽  
Rikky W. Purbojati ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Genome Assembly ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Pseudomonas Sp ◽  
High Quality ◽  
Protein Coding ◽  
Protein Coding Genes ◽  
Air Sample ◽  
High Quality Genome

Pseudomonas sp. strain SGAir0191 was isolated from an air sample collected in Singapore, and its genome was sequenced using a combination of long and short reads to generate a high-quality genome assembly. The complete genome is approximately 5.07 Mb with 4,370 protein-coding genes, 19 rRNAs, and 73 tRNAs.

scholarly journals High-Quality Genome Resource of Clonostachys rosea strain CanS41 by Oxford Nanopore Long-Read Sequencing

Plant Disease ◽  
2021 ◽  
Author(s):  
Haoming Wang ◽  
Yongrong Dong ◽  
Weixue Liao ◽  
Xin Zhang ◽  
Qinhu Wang ◽  
...  
Keyword(s):  
Biological Control ◽  
Genome Assembly ◽  
Plant Pathogens ◽  
Gene Annotation ◽  
Gc Content ◽  
Clonostachys Rosea ◽  
High Quality ◽  
Fungal Plant Pathogens ◽  
Oxford Nanopore ◽  
High Quality Genome

Clonostachys rosea is a necrotrophic mycoparasitic fungus with excellent biological control ability against numerous fungal plant pathogens. Here, we performed genomic sequencing of C. rosea strain CanS41 using Oxford Nanopore sequencing technology. We generated a high-quality genome assembly (>99.99% accuracy), which comprised 26 contigs containing 60.68 Mb sequences with a GC content of 48.55% and a repeat content of 8.38%. The N50 contig length is 3.02 Mb. In total, 20,818 protein-coding genes were identified and functionally annotated. Genes encoding secreted proteins and carbohydrate-active enzymes as well as secondary metabolic gene clusters were also identified and analyzed. In summary, the high-quality genome assembly and gene annotation provided here will allow further exploration of biological functions and enhance biological control ability of C. rosea.

scholarly journals High-Quality Draft Genome Sequence of Fusarium oxysporum f. sp. cubense Strain 160527, a Causal Agent of Panama Disease

2019 ◽  
Vol 8 (29) ◽  
Author(s):  
Shuta Asai ◽  
Yu Ayukawa ◽  
Pamela Gan ◽  
Sachiko Masuda ◽  
Ken Komatsu ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Genome Sequence ◽  
Genome Assembly ◽  
Draft Genome ◽  
Causal Agent ◽  
High Quality ◽  
Panama Disease ◽  
Content Type ◽  
High Quality Genome

Fusarium oxysporum f. sp. cubense is the causal agent of banana Fusarium wilt, also known as Panama disease. Here, we present a high-quality genome sequence of F. oxysporum f. sp. cubense strain 160527. The genome assembly is composed of 12 contigs with a total assembly length of 51,139,495 bp (N 50 contig length, 4,884,632 bp).

High-quality genome assembly of Huazhan and Tianfeng, the parents of an elite rice hybrid Tian-you-hua-zhan

2021 ◽  
Author(s):  
Hui Zhang ◽  
Yuexing Wang ◽  
Ce Deng ◽  
Sheng Zhao ◽  
Peng Zhang ◽  
...  
Keyword(s):  
Genome Assembly ◽  
High Quality ◽  
Rice Hybrid ◽  
High Quality Genome

scholarly journals Whole-Genome Sequencing of Chinese Yellow Catfish Provides a Valuable Genetic Resource for High-Throughput Identification of Toxin Genes

Toxins ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 488 ◽  
Author(s):  
Shiyong Zhang ◽  
Jia Li ◽  
Qin Qin ◽  
Wei Liu ◽  
Chao Bian ◽  
...  
Keyword(s):  
High Throughput ◽  
Genome Assembly ◽  
Raw Materials ◽  
Pelteobagrus Fulvidraco ◽  
Yellow Catfish ◽  
High Quality ◽  
Protein Coding ◽  
Toxin Genes ◽  
Sequencing Platforms ◽  
High Quality Genome

Naturally derived toxins from animals are good raw materials for drug development. As a representative venomous teleost, Chinese yellow catfish (Pelteobagrus fulvidraco) can provide valuable resources for studies on toxin genes. Its venom glands are located in the pectoral and dorsal fins. Although with such interesting biologic traits and great value in economy, Chinese yellow catfish is still lacking a sequenced genome. Here, we report a high-quality genome assembly of Chinese yellow catfish using a combination of next-generation Illumina and third-generation PacBio sequencing platforms. The final assembly reached 714 Mb, with a contig N50 of 970 kb and a scaffold N50 of 3.65 Mb, respectively. We also annotated 21,562 protein-coding genes, in which 97.59% were assigned at least one functional annotation. Based on the genome sequence, we analyzed toxin genes in Chinese yellow catfish. Finally, we identified 207 toxin genes and classified them into three major groups. Interestingly, we also expanded a previously reported sex-related region (to ≈6 Mb) in the achieved genome assembly, and localized two important toxin genes within this region. In summary, we assembled a high-quality genome of Chinese yellow catfish and performed high-throughput identification of toxin genes from a genomic view. Therefore, the limited number of toxin sequences in public databases will be remarkably improved once we integrate multi-omics data from more and more sequenced species.

scholarly journals Genome Assembly and Population Resequencing Reveal the Geographical Divergence of 'Shanmei'(Rubus corchorifolius)

2021 ◽  
Author(s):  
Yinqing Yang ◽  
Kang Zhang ◽  
Ya Xiao ◽  
Lingkui Zhang ◽  
Yile Huang ◽  
...  
Keyword(s):  
Genome Assembly ◽  
Ancestral Population ◽  
Effective Population ◽  
High Quality ◽  
Local Environments ◽  
Rubus Species ◽  
Rubus Chingii ◽  
Rubus Chingii Hu ◽  
Genomic Regions ◽  
High Quality Genome

Rubus corchorifolius (Shanmei or mountain berry, 2n =14) is widely distributed in China, and its fruit has high nutritional and medicinal values. Here, we report a high-quality chromosome-scale genome assembly of Shanmei, with a size of 215.69 Mb and encompassing 26696 genes. Genome comparisons among Rosaceae species show that Shanmei and Fupenzi(Rubus chingii Hu) are most closely related, and then is blackberry (Rubus occidentalis). Further resequencing of 101 samples of Shanmei collected from four regions in provinces of Yunnan, Hunan, Jiangxi and Sichuan in South China reveals that the Hunan population of Shanmei possesses the highest diversity and may represent the relatively more ancestral population. Moreover, the Yunnan population undergoes strong selection based on nucleotide diversity, linkage disequilibrium and the historical effective population size analyses. Furthermore, genes from candidate genomic regions that show strong divergence are significantly enriched in flavonoid biosynthesis and plant hormone signal transduction, indicating the genetic basis of adaptation of Shanmei to the local environments. The high-quality genome sequences and the variome dataset of Shanmei provide valuable resources for breeding applications and for elucidating the genome evolution and ecological adaptation of Rubus species.

scholarly journals Complete High-Quality Genome Sequence of Clostridium limosum (Hathewaya limosa) Isolate 14S0207, Recovered from a Cow with Suspected Blackleg in Germany

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Prasad Thomas ◽  
Mostafa Y. Abdel-Glil ◽  
Anne Busch ◽  
Lothar H. Wieler ◽  
Inga Eichhorn ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Intestinal Tract ◽  
High Quality ◽  
Circular Chromosome ◽  
Content Type ◽  
High Quality Genome

Clostridium limosum can be found in soil and the intestinal tract of animals. In 2014, C. limosum was isolated from a suspected blackleg outbreak in cattle in Schleswig-Holstein, Germany. We present a complete genome sequence of a C. limosum strain represented by a circular chromosome and three plasmids.

scholarly journals Genome Sequence Resource of Magnaporthe oryzae Laboratory Strain 2539

2020 ◽  
Vol 33 (8) ◽  
pp. 1029-1031
Author(s):  
Meilian Chen ◽  
Baohua Wang ◽  
Guodong Lu ◽  
Zhenhui Zhong ◽  
Zonghua Wang
Keyword(s):  
Genome Sequence ◽  
Magnaporthe Oryzae ◽  
Genome Assembly ◽  
Laboratory Strain ◽  
Model System ◽  
Blast Disease ◽  
High Quality ◽  
Host Pathogen Interaction ◽  
Host Pathogen ◽  
High Quality Genome

Magnaporthe oryzae causes blast disease on more than 50 species of monocot plants, including important crops such as rice, millet, and most recently wheat. Additionally, it is an important model system for studying host-pathogen interaction. Here, we report a high-quality genome assembly and annotation of a laboratory strain 2539 of M. oryzae, which is a widely used progeny of a rice-infecting isolate and a grass-infecting isolate. The genome sequence of strain 2539 will be useful for studying the evolution, host adaption, and pathogenicity of M. oryzae, which will be beneficial for a better understanding of the mechanisms of host-pathogen interaction.

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