The glycosylation site in the envelope protein of West Nile virus (Sarafend) plays an important role in replication and maturation processes
2006 ◽
Vol 87
(3)
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pp. 613-622
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Keyword(s):
The complete genome of West Nile (Sarafend) virus [WN(S)V] was sequenced. Phylogenetic trees utilizing the complete genomic sequence, capsid gene, envelope gene and NS5 gene/3′ untranslated region of WN(S)V classified WN(S)V as a lineage II virus. A full-length infectious clone of WN(S)V with a point mutation in the glycosylation site of the envelope protein (pWNS-S154A) was constructed. Both growth kinetics and the mode of maturation were affected by this mutation. The titre of the pWNS-S154A virus was lower than the wild-type virus. This defect was corrected by the expression of wild-type envelope protein in trans. The pWNS-S154A virus matured intracellularly instead of at the plasma membrane as shown for the parental WN(S)V.
2003 ◽
Vol 77
(22)
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pp. 12266-12275
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Keyword(s):
2018 ◽
Vol 5
(suppl_1)
◽
pp. S69-S69
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Keyword(s):
2001 ◽
Vol 75
(19)
◽
pp. 9297-9301
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Keyword(s):
2008 ◽
Vol 89
(12)
◽
pp. 3086-3096
◽
Keyword(s):
2011 ◽
Vol 92
(10)
◽
pp. 2286-2296
◽
Keyword(s):
1999 ◽
Vol 73
(12)
◽
pp. 10164-10172
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Keyword(s):