scholarly journals Preparation and Characterization of Alginate and Psyllium Beads ContainingLactobacillus acidophilus

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Farzaneh Lotfipour ◽  
Shahla Mirzaeei ◽  
Maryam Maghsoodi
Keyword(s):  
Lactobacillus Acidophilus ◽  
Encapsulation Efficiency ◽  
Alginate Beads ◽  
Probiotic Bacteria ◽  
Partial Substitution ◽  
Narrow Size Distribution ◽  
Acidic Conditions ◽  
Ph Conditions

This paper describes preparation and characterization of beads of alginate and psyllium containing probiotic bacteria ofLactobacillus acidophilusDMSZ20079. Twelve different formulations containing alginate (ALG) and alginate-psyllium (ALG-PSL) were prepared using extrusion technique. The prepared beads were characterized in terms of size, morphology and surface properties, encapsulation efficiency, viabilities in acid (pH 1.8, 2 hours) and bile (0.5% w/v, 2 hours) conditions, and release in simulated colon pH conditions. The results showed that spherical beads with narrow size distribution ranging from1.59±0.04to1.67±0.09 mm for ALG and from1.61±0.06to1.80±0.07mm for ALG-PSL with encapsulation efficiency higher than 98% were achieved. Furthermore, addition of PSL into ALG enhanced the integrity of prepared beads in comparison with ALG formulations. The results indicated that incorporation of PSL into alginate beads improved viability of the bacteria in acidic conditions as well as bile conditions. Also, stimulating effect of PSL on the probiotic bacteria was observed through 20-hour incubation in simulated colonic pH solution. According to ourin vitrostudies, PSL can be a suitable polymer candidate for partial substitution with ALG for probiotic coating.

scholarly journals Establishment and Characterization of Stable Zein/Glycosylated Lactoferrin Nanoparticles to Enhance the Storage Stability and in vitro Bioaccessibility of 7,8-Dihydroxyflavone

2022 ◽  
Vol 8 ◽  
Author(s):  
Yufeng Chen ◽  
Xiaojing Gao ◽  
Shucheng Liu ◽  
Qiuxing Cai ◽  
Lijun Wu ◽  
...  
Keyword(s):  
Encapsulation Efficiency ◽  
Storage Stability ◽  
Hydrophobic Interactions ◽  
Amorphous State ◽  
Spherical Shape ◽  
Low Ph ◽  
In Vitro Bioaccessibility ◽  
Ph Conditions

In this work, the lactoferrin (LF) was glycosylated by dextran (molecular weight 10, 40, and 70 kDa, LF 10K, LF 40K, and LF 70K) via Maillard reaction as a stabilizer to establish zein/glycosylated LF nanoparticles and encapsulate 7,8-dihydroxyflavone (7,8-DHF). Three zein/glycosylated LF nanoparticles (79.27–87.24 nm) with low turbidity (<0.220) and polydispersity index (PDI) (<0.230) were successfully established by hydrophobic interactions and hydrogen bonding. Compared with zein/LF nanoparticles, zein/glycosylated LF nanoparticles further increased stability to ionic strength (0–500 mM NaCl) at low pH conditions. Zein/glycosylated LF nanoparticles had nanoscale spherical shape and glycosylated LF changed surface morphology of zein nanoparticles. Besides, encapsulated 7,8-DHF exhibited an amorphous state inside zein/glycosylated LF nanoparticles. Most importantly, zein/glycosylated LF nanoparticles had good water redispersibility, high encapsulation efficiency (above 98.50%), favorable storage stability, and bioaccessibility for 7,8-DHF, particularly LF 40K. Collectively, the above research provides a theoretical reference for the application of zein-based delivery systems.

scholarly journals Antibacterial effect of the adhering human Lactobacillus acidophilus strain LB.

1997 ◽  
Vol 41 (5) ◽  
pp. 1046-1052 ◽  
Author(s):  
M H Coconnier ◽  
V Liévin ◽  
M F Bernet-Camard ◽  
S Hudault ◽  
A L Servin
Keyword(s):  
Lactobacillus Acidophilus ◽  
Culture Supernatant ◽  
Oral Treatment ◽  
Shigella Flexneri ◽  
Lactic Acid Production ◽  
Heat Stable ◽  
Wide Range ◽  
Production Activity ◽  
Acidic Conditions

The spent culture supernatant of the human Lactobacillus acidophilus strain LB produces an antibacterial activity against a wide range of gram-negative and gram-positive pathogens. It decreased the in vitro viability of Staphylococcus aureus, Listeria monocytogenes, Salmonella typhimurium, Shigella flexneri, Escherichia coli, Klebsiella pneumoniae, Bacillus cereus, Pseudomonas aeruginosa, and Enterobacter spp. In contrast, it did not inhibit lactobacilli and bifidobacteria. The activity was heat stable and relatively sensitive to enzymatic treatments and developed under acidic conditions. The antimicrobial activity was independent of lactic acid production. Activity against S. typhimurium SL1344 infecting human cultured intestinal Caco-2 cells was observed as it was in the conventional C3H/He/oujco mouse model with S. typhimurium C5 infection and oral treatment with the LB spent culture supernatant.

Preparation and Characterization of Folate-Decorated Chitosan Microspheres as a Sustained-Release Drugs Carrier

2013 ◽  
Vol 684 ◽  
pp. 57-62 ◽  
Author(s):  
Zhi Hua Xing
Keyword(s):  
Encapsulation Efficiency ◽  
Average Particle Size ◽  
Morphological Characteristics ◽  
Average Particle ◽  
Loading Capacity ◽  
Chitosan Microspheres ◽  
Ionic Crosslinking ◽  
Artificial Gastric Juice

Folic acid-chitosan (FA-CTS) and 10-hydroxycamptothecin (HCPT)-loaded folate-conjugated chitosan (FA-CTS/HCPT) microspheres were prepared by the ionic crosslinking method.The morphological characteristics of microspheres were examined using a scanning electron microscope (SEM). The average particle size and size distribution were determined by dynamic light scattering. The drug encapsulation efficiency (EE) , loading capacity (LC)and release characteristics in vitro were determined using ultraviolet spectrophotometer.The results shown that the microspheres are uniform spherical and regular with a size between 19.79 and81.40μm.Optimized preparation parameters lead to the successful preparation of hydroxycamptothecin-loaded folate-conjugated chitosan microspheres characterized with encapsulation efficiency and loading capacity up to (86.8±0.1)% and 20.6±0.3 % respectively. More then 90% of 10-hydroxycamptothecin was released from microspheres in 4 h at artificial gastric juice, 8h at artificial small intestinal fluid with a good delayed release effect.

scholarly journals Isolation and Characterization of Fusarium moniliforme var. subglutinans from Malformed Mango

1999 ◽  
Vol 4 (2) ◽  
pp. 19
Author(s):  
K.P. Akhtar ◽  
M. Asif ◽  
M.A. Khan ◽  
M.J. Jaskani ◽  
I.A. Khan
Keyword(s):  
Fusarium Moniliforme ◽  
Culture Media ◽  
Isolation And Characterization ◽  
Ph Conditions ◽  
Fungal Association ◽  
Mango Malformation ◽  
Growth Characters ◽  
Better Than

Mango malformation occurs in most mango growing regions of the world. Floral and vegetative malformation have been reported. There is general agreement that the fungal pathogen Fusarium moniliforme var. subglutinans or Fusarium subglutinans is the causal agent. Healthy and malformed samples of both floral and vegetative tissues were collected from different varieties of mango grown in several locations to verify the association of F.moniliforme with mango malformation disease in Pakistan. The fungus was isolated and cultured. Frequency of fungal association with the disease ranged between 90- 94%, There was less recovery of fungus from asymptomatic tissue (12- 15%). There was no difference among the commercial mango varieties in the level of susceptibility to this disease. However, seedling germplasm and land races showing resistance to mango malformation were identified. The in vitro growth characters of the fungus were determined on different culture media, at varying temperatures, light and pH conditions. Mycelial growth on potato dextrose agar was better than nine other media tested. At pH 7.00, the ideal temperature for growth was between 25-30° C. Normally, the malformation is not controlled by fungicide application. The in vitro sensitivity of fungus to six fungicides at three concentrations was determined to seek potential means of chemical control.

scholarly journals Isolation and Characterization of a Bacteriophage Infecting Pseudomonas Aeruginosa and Its Application as a Potential Decontaminating Agent

2021 ◽  
Author(s):  
Sonika Sharma ◽  
Sibnarayan Datta ◽  
Soumya Chatterjee ◽  
Moumita Dutta ◽  
Jhuma Samanta ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Burst Size ◽  
Gc Content ◽  
Alginate Beads ◽  
Open Reading Frames ◽  
Isolation And Characterization ◽  
The Family ◽  
Infected Cells

Abstract To treat antibiotic resistance bacteria, bacteriophage (also called 'phage') application has recently drawn considerable attention from researchers globally. Bacteria like Pseudomonas aeruginosa are known to be associated with nosocomial infections especially in patients with compromised immune systems. In the present work, phage against P. aeruginosa (named 'DRLP1') was isolated from wastewater, enriched and characterized. Morphologically DRLP1 belongs to the family Myoviridae with a high lytic ability. DRLP1 has a burst size of approximately 100 PFU/infected cells, a rapid adsorption time when supplemented with MgCl2, and has viability in a wide temperature range and pH. Genomic sequencing and bioinformatics analysis showed that the phage genome is linear double-stranded, 66,243 bp in length and have a GC content of 54.9%. the genome encodes 93 phage related ORFs open reading frames (ORFs). Phage stability in lyophilized state, adsorption study on sodium alginate beads, and in-vitro pathogen reduction assays were also investigated. Study carried out with artificially contaminated fomites suggests that this phage has the potential for application as a biological decontaminant agent against P. aeruginosa in different conditions.

scholarly journals Preparation and in vitro characterization of gellan based floating beads of acetohydroxamic acid for eradication of H. pylori

2007 ◽  
Vol 57 (4) ◽  
pp. 413-427 ◽  
Author(s):  
Parauvathanahalli Rajinikanth ◽  
Brahmeshwar mishra
Keyword(s):  
Drug Release ◽  
Encapsulation Efficiency ◽  
Diffusion Mechanism ◽  
Oral Dosage ◽  
Acetohydroxamic Acid ◽  
Burst Release ◽  
Sustained Drug Release ◽  
H Pylori

Preparation andin vitrocharacterization of gellan based floating beads of acetohydroxamic acid for eradication ofH. pyloriGellan based floating beads of acetohydroxamic acid (AHA) were prepared by the ionotropic gellation method to achieve controlled and sustained drug release for treatment ofHelicobacter pyloriinfection. The prepared beads were evaluated for diameter, surface morphology and encapsulation efficiency. Formulation parameters like concentrations of gellan, chitosan, calcium carbonate and the drug influenced thein vitrodrug release characteristics of beads. Drug and polymer interaction studies were carried out using differential scanning calorimetry. Chitosan coating increased encapsulation efficiency of the beads and reduced the initial burst release of the drug from the beads. Kinetic treatment of the drug release data revealed a matrix diffusion mechanism. Prepared floating beads showed good antimicrobial activity (in vitro H. pyloriculture) as potent urease inhibitors. In conclusion, an oral dosage form of floating gellan beads containing AHA may form a useful stomach site specific drug delivery system for the treatment ofH. pyloriinfection.

Development of Probiotic Lactobacillus sporogenes Loaded Biological Macromolecules using Novel Assembly Design for Long-term Preservation as Bio-therapeutic Agent

2021 ◽  
Vol 14 (3) ◽  
pp. 5481-5491
Author(s):  
Himanshu Solanki ◽  
Madhavi P. Ghumare ◽  
Vipul D Prajapati ◽  
Sanjeev R Acharya
Keyword(s):  
Encapsulation Efficiency ◽  
Alginate Beads ◽  
Biological Macromolecules ◽  
Automated Production ◽  
Assembly Design ◽  
Intestinal Delivery ◽  
Structure Surface ◽  
Long Term Preservation

The goal present investigation was to formulate and characterized of biological macromolecules of alginate (ALG) and carboxymethylcellulose sodium (CMC) containing probiotic bacteria of Lactobacillus sporogenes (LS) co-encapsulated with a prebiotics, Bioecolians (α-Gluco-oligosaccharides). The prepared beads were characterized in terms of yield, size, encapsulation efficiency, viabilities in simulated gastric (pH 1.2, 2 hours) and bile (1% w/v, 3 hours) conditions. The beads were also characterized by FTIR, DSC, SEM and XRD to investigate molecular structure, surface properties and morphology of beads. The results showed that spherical beads with size distribution ranging from 1.18 ± 0.11 to 1.45 ± 0.15 mm for ALG and from 1.3 ± 0.12 to 1.5 ± 0.16 mm for ALG-CMC with encapsulation efficiency higher than 90% were achieved. The results indicated that incorporation of carboxymethylcellulose sodium into alginate beads improved viability of the bacteria in simulated gastric conditions as well as bile conditions. According to our in vitro studies, Probiotic beads using combination of ALG-CMC are suitable encapsulating polymer for gastro-intestinal delivery as designed by novel assembly using peristaltic pump for automated production.

Development of bozepinib-loaded nanocapsules for nose-to-brain delivery: preclinical evaluation in glioblastoma

Nanomedicine ◽  
2021 ◽  
Author(s):  
Amanda de Fraga Dias ◽  
Danieli Rosane Dallemole ◽  
Franciele Aline Bruinsmann ◽  
Luiz Fernando Lopes Silva ◽  
Olga Cruz-López ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Encapsulation Efficiency ◽  
Brain Delivery ◽  
Particle Sizes ◽  
Potential Treatment ◽  
Preclinical Evaluation ◽  
Glioma Growth

Aim: To develop and characterize bozepinib-loaded lipid-core nanocapsules (BZP-LNC+) as a potential treatment for glioblastoma (GBM). Methods: Characterization of nanocapsules was performed by diameter, polydispersity index, zeta potential, pH and encapsulation efficiency. GBM cell viability, cell cycle and Annexin/PI were evaluated after BZP-LNC+ treatment. Synergism between BZP-LNC+ and temozolomide (TMZ) was performed by CompuSyn software and confirmed in vitro and in vivo. Results: BZP-LNC+ showed adequate particle sizes, positive zeta potential, narrow size distribution and high encapsulation efficiency. BZP-LNC+ reduces GBM growth by inducing apoptosis. BZP-LNC+ and TMZ showed synergistic effect in vitro and reduced the in vivo glioma growth by approximately 81%. Conclusion: The present study provides proof-of-principle insights for the combination of these drugs for GBM treatment.

Survival of encapsulated probiotics in pasteurized grape juice and evaluation of their properties during storage

2018 ◽  
Vol 25 (2) ◽  
pp. 120-129 ◽  
Author(s):  
Samira Mokhtari ◽  
Seid Mahdi Jafari ◽  
Morteza Khomeiri
Keyword(s):  
Lactobacillus Acidophilus ◽  
Storage Period ◽  
Grape Juice ◽  
Alginate Beads ◽  
Bifidobacterium Bifidum ◽  
Probiotic Bacteria ◽  
Internal Gelation ◽  
Free Cells ◽  
Mean Diameter ◽  
Control Samples

In this study, probiotic bacteria such as Lactobacillus acidophilus and Bifidobacterium bifidum were encapsulated in alginate beads with a mean diameter of 54.25 ± 0.18 µm by internal gelation. Encapsulated and free cells as control samples were then added to the pasteurized grape juice and stored for 60 days. At the end of the storage period, the survivability of the bacteria in the encapsulated samples was significantly ( P<0.05) higher than that in the free cells (8.67 ± 0.12 and 7.57 ± 0.08 log cfu mL−1 for L. acidophilus and 8.27 ± 0.05 and 7.53 ± 0.07 log cfu mL−1 for B. bifidum for encapsulated and free forms, respectively). The results generally showed a decrease in °Brix, pH, and color, whereas acidity and turbidity have increased the in probiotic grape juice after the storage period of 60 days. For all treatments, the numbers of surviving cells were more than the recommended minimum (107 cfu g−1) at the end of the storage period.

scholarly journals Development, in vitro and in vivo evaluations of novel lipid drug delivery system of Newbouldia laevis (P. Beauv.)

2016 ◽  
Vol 3 ◽  
pp. 184954351667344
Author(s):  
Chukwuebuka Umeyor ◽  
Emmanuel Anaka ◽  
Franklin Kenechukwu ◽  
Chinazom Agbo ◽  
Anthony Attama
Keyword(s):  
Encapsulation Efficiency ◽  
Folk Medicine ◽  
Research Work ◽  
Lipid Matrix ◽  
Solid Lipid ◽  
Development In Vitro

Newbouldia laevis (P. Beauv.) is a tropical rainforest plant used in traditional folk medicine for the treatment of malaria, cough, joint pains, stomach ache, oedema and inflammation. The main thrust of this research work was to study the analgesic/anti-nociceptive properties of N. laevis-loaded solid lipid microdispersions. N. laevis leaves were extracted using ethanol, and the extract was formulated into solid lipid microdispersions using lipid matrix comprising a rational blend of Precirol® ATO 5 and Softisan® 154. Characterization of the solid lipid microdispersions include determination of morphology, particle size, pH, thermal property, encapsulation efficiency percentage and analgesic/anti-nociceptive property. The results obtained showed that the particles were spherical with sizes ranging from 40 µm to 125 µm. The solid lipid microdispersions maintained a stable pH within the acidic region of 5–6 with insignificant variations ( p > 0.05) over a period of 90 days. Thermal analysis showed that N. laevis was entrapped in the lipid matrix used for the formulations. Solid lipid microdispersions recorded a maximum encapsulation efficiency up to 88.1%. N. laevis-loaded solid lipid microdispersions also produced good analgesic/anti-nociceptive property comparable with the standard diclofenac potassium. N. laevis-loaded solid lipid microdispersions showed good analgesic/anti-nociceptive effect and could be used in the treatment and management of pain.

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