scholarly journals Cell-type heterogeneity in the zebrafish olfactory placode is generated from progenitors within preplacodal ectoderm

2017 ◽  
Author(s):  
Raphaël Aguillon ◽  
Julie Batut ◽  
Arul Subramanian ◽  
Romain Madelaine ◽  
Pascale Dufourcq ◽  
...  
Keyword(s):  
Neural Crest ◽  
Sensory Neurons ◽  
Time Lapse ◽  
Gonadotropin Releasing Hormone ◽  
Lineage Tracing ◽  
Cranial Neural Crest ◽  
Cell Type ◽  
Olfactory Placode ◽  
Cell Numbers ◽  
Neuronal Populations

AbstractVertebrate olfactory placodes consists of a variety of neuronal populations, which are thought to have distinct embryonic origins. In the zebrafish, while ciliated sensory neurons arise from preplacodal ectoderm (PPE), previous lineage tracing studies suggest that both Gonadotropin releasing hormone 3 (Gnrh3) and microvillous sensory neurons derive from cranial neural crest (CNC). We find that the expression of Islet1/2 is restricted to Gnrh3 neurons associated with the olfactory placode. Unexpectedly, however, we find no change in Islet1/2+ cell numbers in sox10 mutant embryos, calling into question their CNC origin. Lineage reconstruction based on backtracking in time-lapse confocal datasets, and confirmed by photoconversion experiments, reveals that Gnrh3 neurons derive from the anterior/medial PPE. Similarly, all of the microvillous sensory neurons we have traced arise from preplacodal progenitors. Our results suggest that rather than originating from separate ectodermal populations, cell-type heterogeneity is generated from overlapping pools of progenitors within the preplacodal ectoderm.

scholarly journals Cell-type heterogeneity in the early zebrafish olfactory epithelium is generated from progenitors within preplacodal ectoderm

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Raphaël Aguillon ◽  
Julie Batut ◽  
Arul Subramanian ◽  
Romain Madelaine ◽  
Pascale Dufourcq ◽  
...  
Keyword(s):  
Neural Crest ◽  
Sensory Neurons ◽  
Olfactory Epithelium ◽  
Time Lapse ◽  
Gonadotropin Releasing Hormone ◽  
Lineage Tracing ◽  
Cranial Neural Crest ◽  
Cell Type ◽  
Cell Numbers ◽  
Neuronal Populations

The zebrafish olfactory epithelium comprises a variety of neuronal populations, which are thought to have distinct embryonic origins. For instance, while ciliated sensory neurons arise from preplacodal ectoderm (PPE), previous lineage tracing studies suggest that both Gonadotropin releasing hormone 3 (Gnrh3) and microvillous sensory neurons derive from cranial neural crest (CNC). We find that the expression of Islet1/2 is restricted to Gnrh3 neurons associated with the olfactory epithelium. Unexpectedly, however, we find no change in Islet1/2+ cell numbers in sox10 mutant embryos, calling into question their CNC origin. Lineage reconstruction based on backtracking in time-lapse confocal datasets, and confirmed by photoconversion experiments, reveals that Gnrh3 neurons derive from the anterior PPE. Similarly, all of the microvillous sensory neurons we have traced arise from preplacodal progenitors. Our results suggest that rather than originating from separate ectodermal populations, cell-type heterogeneity is generated from overlapping pools of progenitors within the preplacodal ectoderm.

scholarly journals Sox10-dependent neural crest origin of olfactory microvillous neurons in zebrafish

eLife ◽  
2013 ◽  
Vol 2 ◽  
Author(s):  
Ankur Saxena ◽  
Brian N Peng ◽  
Marianne E Bronner
Keyword(s):  
Neural Crest ◽  
Sensory Neurons ◽  
Olfactory Epithelium ◽  
Cell Tracking ◽  
Function Analysis ◽  
Critical Role ◽  
Primary Source ◽  
Olfactory Sensory Neurons ◽  
Cranial Neural Crest ◽  
Loss Of Function

The sense of smell in vertebrates is detected by specialized sensory neurons derived from the peripheral nervous system. Classically, it has been presumed that the olfactory placode forms all olfactory sensory neurons. In contrast, we show that the cranial neural crest is the primary source of microvillous sensory neurons within the olfactory epithelium of zebrafish embryos. Using photoconversion-based fate mapping and live cell tracking coupled with laser ablation, we followed neural crest precursors as they migrated from the neural tube to the nasal cavity. A subset that coexpressed Sox10 protein and a neurogenin1 reporter ingressed into the olfactory epithelium and differentiated into microvillous sensory neurons. Timed loss-of-function analysis revealed a critical role for Sox10 in microvillous neurogenesis. Taken together, these findings directly demonstrate a heretofore unknown contribution of the cranial neural crest to olfactory sensory neurons in zebrafish and provide important insights into the assembly of the nascent olfactory system.

Delta signaling mediates segregation of neural crest and spinal sensory neurons from zebrafish lateral neural plate

Development ◽  
2000 ◽  
Vol 127 (13) ◽  
pp. 2873-2882 ◽  
Author(s):  
R.A. Cornell ◽  
J.S. Eisen
Keyword(s):  
Neural Crest ◽  
Point Mutation ◽  
Sensory Neurons ◽  
Genetic Difference ◽  
Neural Plate ◽  
Cranial Neural Crest ◽  
Neural Crest Development ◽  
Neural Crest Derivative ◽  
Trunk Neural Crest

We examined the role of Delta signaling in specification of two derivatives in zebrafish neural plate: Rohon-Beard spinal sensory neurons and neural crest. deltaA-expressing Rohon-Beard neurons are intermingled with premigratory neural crest cells in the trunk lateral neural plate. Embryos homozygous for a point mutation in deltaA, or with experimentally reduced delta signalling, have supernumerary Rohon-Beard neurons, reduced trunk-level expression of neural crest markers and lack trunk neural crest derivatives. Fin mesenchyme, a putative trunk neural crest derivative, is present in deltaA mutants, suggesting it segregates from other neural crest derivatives as early as the neural plate stage. Cranial neural crest derivatives are also present in deltaA mutants, revealing a genetic difference in regulation of trunk and cranial neural crest development.

scholarly journals Close association of olfactory placode precursors and cranial neural crest cells does not predestine cell mixing

2012 ◽  
Vol 241 (7) ◽  
pp. 1143-1154 ◽  
Author(s):  
Maegan V. Harden ◽  
Luisa Pereiro ◽  
Mirana Ramialison ◽  
Jochen Wittbrodt ◽  
Megana K. Prasad ◽  
...  
Keyword(s):  
Neural Crest ◽  
Close Association ◽  
Neural Crest Cells ◽  
Cranial Neural Crest ◽  
Olfactory Placode ◽  
Cranial Neural Crest Cells

scholarly journals Clonal analysis and dynamic imaging identify multipotency of individual Gallus gallus caudal hindbrain neural crest cells toward cardiac and enteric fates

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Weiyi Tang ◽  
Yuwei Li ◽  
Ang Li ◽  
Marianne E. Bronner
Keyword(s):  
Neural Crest ◽  
Single Cell ◽  
Cell Fate ◽  
Neural Crest Cells ◽  
Time Lapse ◽  
Cell Types ◽  
Clonal Analysis ◽  
Dynamic Imaging ◽  
Lineage Tracing ◽  
Enteric Ganglia

AbstractNeural crest stem cells arising from caudal hindbrain (often called cardiac and posterior vagal neural crest) migrate long distances to form cell types as diverse as heart muscle and enteric ganglia, abnormalities of which lead to common congenital birth defects. Here, we explore whether individual caudal hindbrain neural crest precursors are multipotent or predetermined toward these particular fates and destinations. To this end, we perform lineage tracing of chick neural crest cells at single-cell resolution using two complementary approaches: retrovirally mediated multiplex clonal analysis and single-cell photoconversion. Both methods show that the majority of these neural crest precursors are multipotent with many clones producing mesenchymal as well as neuronal derivatives. Time-lapse imaging demonstrates that sister cells can migrate in distinct directions, suggesting stochasticity in choice of migration path. Perturbation experiments further identify guidance cues acting on cells in the pharyngeal junction that can influence this choice; loss ofCXCR4signaling results in failure to migrate to the heart but no influence on migration toward the foregut, whereas loss ofRETsignaling does the opposite. Taken together, the results suggest that environmental influences rather than intrinsic information govern cell fate choice of multipotent caudal hindbrain neural crest cells.

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