scholarly journals Bioconversion of Mango Pulp Industrial Waste into Ellagic acid Using Aspergillus niger

2020 ◽  
Author(s):  
Athiappan Murugan ◽  
Anandan Rubavathi ◽  
Visali Kannan ◽  
Aurumugam Parthiban
Keyword(s):  
Aspergillus Niger ◽  
Carbon Source ◽  
Ellagic Acid ◽  
Acid Production ◽  
Bioactive Compound ◽  
Ft Ir ◽  
Ir Analysis ◽  
Pulp Processing ◽  
Mango Pulp ◽  
Plant Sources

AbstractEllagic acid was considered as the potential bioactive compound with many therapeutical applications. Bioconversion of tannin present in the mango pulp processing waste in to ellagic acid using fungi would be better alternate than the chemical as well as extraction from plant sources. A total of three different fungi were isolated from the soil sample and it was confirmed as Aspergillus niger. Further, the isolated strains of A. niger were identified to produce ellagic acid from ellagitannin of mango waste. Quantification of the ellagic acid production was carried out by solid-state fermentation using 3% of mango waste as substrate. Ellagic acid enzyme activity was calculated and found to be 17.6 U ml−1 The ellagic acid production was optimized to fix the various factors, that is, pH and temperature, nitrogen and carbon source. The maximum production (200 μg/g) of ellagic acid was achieved at pH 5.5, temperature 30 °C, Ammonium nitrate as nitrogen source, 0.2% of NaCl and carbon source (0.2% of sugar) with 3% of mango pulp waste. Ellagic acid produced was characterized by UV–vis spectrophotometer and by FT-IR analysis.

scholarly journals Enhancing or Inhibitory Effect of Fruit or Vegetable Bioactive Compound on Aspergillus niger and A. oryzae

2021 ◽  
Vol 8 (1) ◽  
pp. 12
Author(s):  
Gülru Bulkan ◽  
Sitaresmi Sitaresmi ◽  
Gerarda Tania Yudhanti ◽  
Ria Millati ◽  
Rachma Wikandari ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Aspergillus Niger ◽  
Bioactive Compounds ◽  
Ellagic Acid ◽  
Microbial Growth ◽  
Biomass Yield ◽  
Antimicrobial Effect ◽  
Bioactive Compound ◽  
Inhibitory Effect ◽  
Processing Wastes

Fruit and vegetable processing wastes are global challenges but also suitable sources with a variety of nutrients for different fermentative products using bacteria, yeast or fungi. The interaction of microorganisms with bioactive compounds in fruit waste can have inhibitory or enhancing effect on microbial growth. In this study, the antimicrobial effect of 10 bioactive compounds, including octanol, ellagic acid, (−)-epicatechin, quercetin, betanin, ascorbic acid, limonene, hexanal, car-3-ene, and myrcene in the range of 0–240 mg/L on filamentous fungi Aspergillus oryzae and Aspergillus niger were investigated. These fungi were both found to be resistant to all compounds except octanol, which can be used as a natural antifungal agent, specifically against A. oryzae and A. niger contamination. On the contrary, polyphenols (quercetin and ellagic acid), ascorbic acid, and hexanal enhanced A. niger biomass yield 28%, 7.8%, 16%, and 6%, respectively. Furthermore, 240 mg/L car-3-ene was found to increase A. oryzae biomass yield 8%, while a 9% decrease was observed at lower concentration, 24 mg/L. Similarly, up to 17% decrease of biomass yield was observed from betanin and myrcene. The resistant nature of the fungi against FPW bioactive compounds shows the potential of these fungi for further application in waste valorization.

Ellagic acid production by Aspergillus niger in solid state fermentation of pomegranate residues

2008 ◽  
Vol 35 (6) ◽  
pp. 507-513 ◽  
Author(s):  
Armando Robledo ◽  
Antonio Aguilera-Carbó ◽  
Raúl Rodriguez ◽  
José Luis Martinez ◽  
Yolanda Garza ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Ellagic Acid ◽  
Acid Production

scholarly journals Effect of some physiological factors on citric acidproduction bythreeisolates ofAspergillus niger acid - hydrolyzed sawdust as a carbon source

2018 ◽  
Vol 6 (2) ◽  
pp. 1-9
Author(s):  
Muna, B. Abdulazeez ◽  
Mustafa M. Haider
Keyword(s):  
Citric Acid ◽  
Aspergillus Niger ◽  
Carbon Source ◽  
Acid Production ◽  
Large Scale ◽  
Nitrogen Concentration ◽  
Tricarboxylic Acid ◽  
Ion Concentration ◽  
Citric Acid Production ◽  
Hydrogen Ion Concentration

Introduction: Citric acid (2-hydroxy-propane-1, 2, 3-tricarboxylic acid) was first isolated from lemon juice in 1784. It is a primary metabolic product which is formed in the tricarboxylic acid (Krebs) cycle. It is estimated that the market value of citric acid will exceed two billion dollars in 2019. About 70% of total citric acid produced globally is utilized in food industry, while about 12% is utilized in pharmaceuticals and cosmetic industries and the remainder in other industrial purposes. The industrial production of citric acid is undertaken by fermentation process in the presence of filamentous fungi for large scale of production. Aspergillus niger is the most efficient fungus due to its ability to produce more citric acid per unit time and ferment different inexpensive raw materials. Materials and Methods: Three isolates of the fungus Aspergillus niger (An1, An2, An3) were used throughout this study using different carbon source concentration in the form of sawdust acid hydrolysis supplemented with different concentration of (NH4)2H2SO4 as a nitrogen source. The effect of hydrogen ion concentration and addition of methanol to the fermentation medium was also investigated. Results and Discussion: The results indicated that the optimization of carbon and nitrogen concentration had stimulatingeffect on citric acid production by the three used isolates. Moreover, addition of methanol at concentration of 1% at pH of 3.5 highly increased citric acid production. Conclusion: we concluded that the agriculture waste was a favorable substrate for the production of citric acid especially it is cost effective and easily obtainable.

Ellagic Acid Production from Biodegradation of Creosote Bush Ellagitannins by Aspergillus niger in Solid State Culture

2008 ◽  
Vol 2 (2) ◽  
pp. 208-212 ◽  
Author(s):  
Antonio Aguilera-Carbo ◽  
Juan S. Hernández ◽  
Christopher Augur ◽  
Lilia A. Prado-Barragan ◽  
Ernesto Favela-Torres ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Ellagic Acid ◽  
Acid Production ◽  
Creosote Bush ◽  
State Culture ◽  
Solid State Culture

scholarly journals Microbial Production Of Ellagic Acid From Mango Pulp Processing Waste

2020 ◽  
Author(s):  
Anandan Rubavathi ◽  
Athiappan Murugan ◽  
Kannan Visali
Keyword(s):  
Ellagic Acid ◽  
Micrococcus Luteus ◽  
Microbial Production ◽  
Chemical Extraction ◽  
Glucose Carbon ◽  
Potential Applications ◽  
Anti Oxidant ◽  
Parasitic Activity ◽  
Pulp Processing ◽  
Mango Pulp

AbstractEllagic acid has gained momentum recently due to its various properties like anti-mutagenic, anti-carcinogenic, anti-oxidant, and anti-viral and many other benefits to human health. The present study focused on the microbial production of ellagic acid from mango pulp processing industrial waste an alternate method for conventional chemical extraction. Our experiments demonstrated that the 100 μg/ml of ellagic acid was produced by Micrococcus luteus from 9% of mango pulp waste and the optimization of ellagic acid production with Pontecorvo medium supplemented with 5.0 g of ellagitannin has yielded 37.80 ± 0.30 mg/g at pH 5.0, temperature 30 °C, ammonium nitrate (nitrogen source), glucose (carbon source), with 1.5% of inoculums after 24 h of incubation. Ellagic acid synthesized was further confirmed with the standard ellagic acid. Applications like drought resistant in plants, anti-microbial activity, anti-parasitic activity and anti-cancer activities have been proven. Ellagic acid exhibited potential applications and further research in product development is promising.

Exploring the Degradation of Gallotannins Catalyzed by Tannase Produced by Aspergillus niger GH1 for Ellagic Acid Production in Submerged and Solid-State Fermentation

2017 ◽  
Vol 185 (2) ◽  
pp. 476-483 ◽  
Author(s):  
Mónica L. Chávez-González ◽  
Sylvain Guyot ◽  
Raul Rodríguez-Herrera ◽  
Arely Prado-Barragán ◽  
Cristóbal N. Aguilar
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Ellagic Acid ◽  
Acid Production ◽  
Aspergillus Niger Gh1

Polarized microbeam FT-IR analysis of single fibers

1996 ◽  
Vol 54 ◽  
pp. 206-207
Author(s):  
Liling Cho ◽  
David L. Wetzel
Keyword(s):  
Molecular Orientation ◽  
Transition Point ◽  
Polymer Fibers ◽  
Single Fiber ◽  
Wire Grid ◽  
Polyester Fibers ◽  
Ft Ir ◽  
Ir Analysis ◽  
The Relationship ◽  
Wire Grid Polarizer

Polarized infrared microscopy has been used for forensic purposes to differentiate among polymer fibers. Dichroism can be used to compare and discriminate between different polyester fibers, including those composed of polyethylene terephthalate that are frequently encountered during criminal casework. In the fiber manufacturering process, fibers are drawn to develop molecular orientation and crystallinity. Macromolecular chains are oriented with respect to the long axis of the fiber. It is desirable to determine the relationship between the molecular orientation and stretching properties. This is particularly useful on a single fiber basis. Polarized spectroscopic differences observed from a single fiber are proposed to reveal the extent of molecular orientation within that single fiber. In the work presented, we compared the dichroic ratio between unstretched and stretched polyester fibers, and the transition point between the two forms of the same fiber. These techniques were applied to different polyester fibers. A fiber stretching device was fabricated for use on the instrument (IRμs, Spectra-Tech) stage. Tension was applied with a micrometer screw until a “neck” was produced in the stretched fiber. Spectra were obtained from an area of 24×48 μm. A wire-grid polarizer was used between the source and the sample.

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