Enhancing or Inhibitory Effect of Fruit or Vegetable Bioactive Compound on Aspergillus niger and A. oryzae

Fruit and vegetable processing wastes are global challenges but also suitable sources with a variety of nutrients for different fermentative products using bacteria, yeast or fungi. The interaction of microorganisms with bioactive compounds in fruit waste can have inhibitory or enhancing effect on microbial growth. In this study, the antimicrobial effect of 10 bioactive compounds, including octanol, ellagic acid, (−)-epicatechin, quercetin, betanin, ascorbic acid, limonene, hexanal, car-3-ene, and myrcene in the range of 0–240 mg/L on filamentous fungi Aspergillus oryzae and Aspergillus niger were investigated. These fungi were both found to be resistant to all compounds except octanol, which can be used as a natural antifungal agent, specifically against A. oryzae and A. niger contamination. On the contrary, polyphenols (quercetin and ellagic acid), ascorbic acid, and hexanal enhanced A. niger biomass yield 28%, 7.8%, 16%, and 6%, respectively. Furthermore, 240 mg/L car-3-ene was found to increase A. oryzae biomass yield 8%, while a 9% decrease was observed at lower concentration, 24 mg/L. Similarly, up to 17% decrease of biomass yield was observed from betanin and myrcene. The resistant nature of the fungi against FPW bioactive compounds shows the potential of these fungi for further application in waste valorization.

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Bioconversion of Mango Pulp Industrial Waste into Ellagic acid Using Aspergillus niger

10.1101/2020.03.17.996074 ◽

2020 ◽

Author(s):

Athiappan Murugan ◽

Anandan Rubavathi ◽

Visali Kannan ◽

Aurumugam Parthiban

Keyword(s):

Aspergillus Niger ◽

Carbon Source ◽

Ellagic Acid ◽

Acid Production ◽

Bioactive Compound ◽

Ft Ir ◽

Ir Analysis ◽

Pulp Processing ◽

Mango Pulp ◽

Plant Sources

AbstractEllagic acid was considered as the potential bioactive compound with many therapeutical applications. Bioconversion of tannin present in the mango pulp processing waste in to ellagic acid using fungi would be better alternate than the chemical as well as extraction from plant sources. A total of three different fungi were isolated from the soil sample and it was confirmed as Aspergillus niger. Further, the isolated strains of A. niger were identified to produce ellagic acid from ellagitannin of mango waste. Quantification of the ellagic acid production was carried out by solid-state fermentation using 3% of mango waste as substrate. Ellagic acid enzyme activity was calculated and found to be 17.6 U ml−1 The ellagic acid production was optimized to fix the various factors, that is, pH and temperature, nitrogen and carbon source. The maximum production (200 μg/g) of ellagic acid was achieved at pH 5.5, temperature 30 °C, Ammonium nitrate as nitrogen source, 0.2% of NaCl and carbon source (0.2% of sugar) with 3% of mango pulp waste. Ellagic acid produced was characterized by UV–vis spectrophotometer and by FT-IR analysis.

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The Antibacterial Activity of Thermoactinomyces sp. (H24) Extract Against Escherichia coli and Staphylococcus aureus

Indonesian Journal of Medical Laboratory Science and Technology ◽

10.33086/ijmlst.v3i1.1700 ◽

2021 ◽

Vol 3 (1) ◽

pp. 56-63

Author(s):

Julia Nanda Puspita ◽

Rikhsan Kurniatuhadi ◽

Rahmawati Rahmawati

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Bioactive Compounds ◽

Bioactive Compound ◽

Inhibitory Effect ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

And Staphylococcus Aureus

Bacteria of the genus Thermoactinomyces have the ability to produce antibacterial bioactive compounds. This bioactive compound can be used for combating diarrheal agents such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antibacterial activity of the metabolite extract from Thermoactinomyces sp. (H24) against Escherichia coli and Staphylococcus aureus. Methanol was used as a solvent for the extraction of bacterial bioactive compounds. Antibacterial activity was analyzed by the diffusion method with several extract concentrations (0.75 mL, 1.5 mL, 2.25 mL, and 3 mL), 10% DMSO as the negative control, and ciprofloxacin as the positive control. Our result shows that Termoactinomyces sp. (H24) extract has an inhibitory effect on the growth of E. coli and S. aureus with an effective concentration of 2.25 mL (inhibition strength: very strong).

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Bioactive compound retention in frozen red and yellow Strawberry guava pulps added with L-Ascorbic acid

Revista Brasileira de Fruticultura ◽

10.1590/0100-29452018032 ◽

2018 ◽

Vol 40 (6) ◽

Cited By ~ 1

Author(s):

Lisiane Pintanela Vergara ◽

Gabriela Niemeyer Reissig ◽

Rodrigo Cezar Franzon ◽

Rosane da Silva Rodrigues ◽

Josiane Freitas Chim

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Bioactive Compounds ◽

Frozen Storage ◽

Bioactive Compound ◽

Freezing Process ◽

Limiting Factor ◽

Protective Agent ◽

Total Carotenoids ◽

Total Anthocyanins

Abstract Brazil presents a great variety of native fruits, which have aroused commercial interest, such as the strawberry guava. In this sense, many researches have looked for alternatives that allow postharvest availability of fruits, considering that perishability is a limiting factor for its commercialization. The freezing process is an important alternative in the sense of making these fruits available for longer on the market. The use of L-ascorbic acid is a potent protective agent in the stability of bioactive compounds in fruit pulps. The objective of this study was to evaluate potentially bioactive compounds present in red and yellow strawberry guava pulps added with L-ascorbic acid (1% m / m) and verify its effect on retention of these compounds and in its antioxidant activity during the frozen storage (-20 ± 2ºC). In the pulps were analyzed: antioxidant activity by the capture of the DPPH° and ABTS° radicals, phenols, anthocyanins and total carotenoids. L-ascorbic acid positively influences the retention of bioactive compounds (total phenols, total anthocyanins and total carotenoids) in red and yellow strawberry guava pulps during 120 days of frozen storage (-20 ± 2 ºC).

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Chemical Preservatives Action on Microbial Growth in a Model System of Refrigerated Prepeeled Potatoes

Journal of Food Protection ◽

10.4315/0362-028x-56.9.801 ◽

1993 ◽

Vol 56 (9) ◽

pp. 801-807 ◽

Cited By ~ 15

Author(s):

L. GIANNUZZI ◽

N. E. ZARITZKY

Keyword(s):

Ascorbic Acid ◽

Citric Acid ◽

Storage Time ◽

Microbial Growth ◽

Total Concentration ◽

Inhibitory Effect ◽

Model System ◽

Sodium Bisulfite ◽

Chemical Preservatives ◽

Aerobic And Anaerobic

The effect of different concentrations of citric acid and ascorbic acid (applied individually or in mixture's) on microbial growth in potato homogenate was analyzed and compared to the sodium bisulfite action during storage at 4°C in low gaseous permeability films. These experiments allowed one to simulate the behavior of prepeeled potatoes but with a known amount of added preservative to evaluate additive or synergic effects. Total viable microorganisms, Enterobacteriaceae, Pseudomonas sp., Lactobacillus sp., molds, yeasts, Clostridium sulfite reducers, psychrotropic microorganisms, and aerobic and anaerobic viable spores were analyzed during storage time. Inhibition indexes produced by the tested preservatives were calculated for the different microorganisms. Sodium bisulfite solutions (100 ppm) had no inhibitory effect. Concentrations of 3,500 ppm citric acid and 10,000 ppm ascorbic acid showed antimicrobial action as well as mixtures of citric acid/ascorbic acid of the following compositions (in terms of total acids concentration): 2,700/2,000, 3,500/2,000, and 2,700/3,000 ppm. A higher effect on Enterobacteriaceae was observed in comparison with other microorganisms. The apparent synergic effect of these acids when they were applied together was demonstrated to be actually an additive effect when concentrations of undissociated acid in the mixtures were considered instead of total concentration.

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Screening of Major Bioactive Compounds Profile of Pomegranate Peel Waste by Using HPLC

10.21203/rs.3.rs-1068729/v1 ◽

2021 ◽

Author(s):

Nishant Kumar ◽

Pratibha ◽

Neeraj ◽

Rokayya Sami ◽

Ebtihal Khojah ◽

...

Keyword(s):

Gallic Acid ◽

Bioactive Compounds ◽

Ellagic Acid ◽

Freeze Drying ◽

Animal Health ◽

Bioactive Compound ◽

The Other ◽

Drying Methods ◽

Pomegranate Peel ◽

Freeze Dried

Abstract Bioactive compound characterization is an important steps for utilization of pomegranate peel waste as food and nutraceutical ingredients. In the present investigation the effects of different drying (freeze, tray-oven and sun) and solvents such as methanol, ethanol, water, acetone and hexane were investigated on the extraction and recovery of major bioactive compounds (ellagic acid, gallic acid, quercetin and punicalagin) of pomegranate peel for two pomegranate varieties (i.e. Bhagwa and Ganesh) using high performance liquid chromatography (HPLC). The results indicated that the peel powder obtained by freeze drying (-45°C) method which extracted by methanol was higher amount in bioactive compounds compared to the other drying methods and solvents. Freeze dried peel powder of Bhagwa pomegranate showed higher amount of gallic acid (32.2 mg/g), ellagic acid (13.6 mg/g), punicalagin (15.2 mg/g) and quercetin (2.5 mg/g) with methanol solvent as compared to the other extract of Bhagwa and Ganesh varieties. Basis on the results of current study it can be concluded that the freeze drying method for drying pomegranate peel powder and methanol as a solvent are effectives to recover higher extraction of bioactive compounds which can be utilize to develop human and animal health.

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Bioactive Compound Contents and Antioxidant Activity of of Fermented Lead Tree (Leucaena leucocephala (lmk.) de Wit) Seeds

Molekul ◽

10.20884/1.jm.2021.16.3.756 ◽

2021 ◽

Vol 16 (3) ◽

pp. 194

Author(s):

Venty Suryanti ◽

Soerya Dewi Marliyana ◽

Gracia Lasma Rohana ◽

Elyna Wahyu Trisnawati ◽

Widiyanti Widiyanti

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Petroleum Ether ◽

Bioactive Compounds ◽

Leucaena Leucocephala ◽

Bioactive Compound ◽

Total Phenolic ◽

Tree Seeds ◽

Β Carotene ◽

Tree Seed

Leucaena leucocephala (lmk.) de Wit) (leed tree) seeds have been fermented by Indonesian as a traditional food which is called tempeh. The fermentation products of legume seeds are good functional foods, since they contain many bioactive compounds. The objective of this study was to determine the bioactive compound contents of fermented lead tree seed, such as total phenolic, ascorbic acid, β-carotene and a-tocopherol. Antioxidant activity testing was carried out using the thiobarbituric acid reactive substances (TBARS) method. Analysis of total phenolics, ascorbic acid, β-carotene and a-tocopherol were carried out using the folin-ciocalteu method, iodine titration, spectrophotometry and iron (III) chloride-thiocyanate, respectively. Rhizopus oligosporus was used to ferment lead tree seeds for 0, 1, 2, 3 and 4 days. Fermented leed tree seed powder was extracted with petroleum ether followed by ethanol. In comparison to unfermented leed tree seed powder, the β-carotene and a-tocopherol of the fermented seed powder increased. The greatest enhancement of β-carotene and a-tocopherol contents were observed at day 2 of fermentation. The highest antioxidant activities of fermented leed tree seeds were also obained at day 2 of fermentation with 55.55±4.59 and 74.46±3.49% inhibition in petroleum ether and ethanol extracts, respectively. The results show that lead tree seed tempeh has high antioxidant activity due to the bioactive compounds contains, such as phenolics, β-carotene and a-tocopherol. Therefore, leed tree seed tempeh could be used for possible commercial production of functional food to alleviate oxidative stress.

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Effects of Ellagic Acid upon the Rabbit Fibrinolytic System

Thrombosis and Haemostasis ◽

10.1055/s-0038-1649010 ◽

1972 ◽

Vol 27 (01) ◽

pp. 063-071

Author(s):

S. G Iatridis ◽

P. G Iatridis

Keyword(s):

Continuous Infusion ◽

Ellagic Acid ◽

Animal Experimentation ◽

Inhibitory Effect ◽

In Vivo Studies ◽

Clot Lysis ◽

Hageman Factor ◽

Lysis Activity ◽

Site Of Action

SummaryThe present investigation deals with in vivo studies of possible relations of active Hageman factor (HFa) to the problems of thrombolysis. The study is based upon animal experimentation in which 40 normal, 5 dicumarolized and 5 heparinized rabbits each received ellagic acid (Elac 10-2 M) by intravenous continuous infusion at a rate of 1 ml/min for a period of 25 min. The data suggest that the Elac infusion induced in vivo activation of HF. Streptokinase (SK) injection 25 min from the start of Elac i. v. infusion failed to induce clot lysis in blood drawn one min after its injection. The phenomenon was more prominent with low (SK 250 U or 500 U) concentrations of SK. With higher concentrations, SK-induced clot lysis activity was not affected by Elac infusion.In dicumarolized and heparinized rabbits Elac infusion still counteracted the fibrinolysis activating effect of low concentration of SK. The possibility that the above described phenomenon was due to either hypercoagulability or to a non-specific inhibitory effect of Elac upon SK was explored and excluded.It is concluded that HFa and SK have the same site of action. Thus it seems that HFa may block the precursor upon which SK acts by forming a complex with it. It is stressed that activation of this precursor by HFa requires a suitable surface.

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Inhibitory Effect of Ascorbic Acid 2-O-.ALPHA.-Glucoside on the Pigmentation of Skin by Exposure to Ultraviolet Light.

Nishi Nihon Hifuka ◽

10.2336/nishinihonhifu.58.439 ◽

1996 ◽

Vol 58 (3) ◽

pp. 439-443 ◽

Cited By ~ 3

Author(s):

Eriko MIYAI ◽

Itaru YAMAMOTO ◽

Jun-ichi AKIYAMA ◽

Mitsuhiro YANAGIDA

Keyword(s):

Ascorbic Acid ◽

Ultraviolet Light ◽

Inhibitory Effect

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In Vitro Coliform Resistance to Bioactive Compounds in Urinary Infection, Assessed in a Lab Catheterization Model

Applied Sciences ◽

10.3390/app11094315 ◽

2021 ◽

Vol 11 (9) ◽

pp. 4315

Author(s):

Emanuel Vamanu ◽

Laura Dorina Dinu ◽

Cristina Mihaela Luntraru ◽

Alexandru Suciu

Keyword(s):

Urinary Tract ◽

Bioactive Compounds ◽

Urinary Tract Infections ◽

Antimicrobial Effect ◽

Biologically Active ◽

Bacterial Strains ◽

E Coli ◽

Functional Product ◽

Tract Infections

Bioactive compounds and phenolic compounds are viable alternatives to antibiotics in recurrent urinary tract infections. This study aimed to use a natural functional product, based on the bioactive compounds’ composition, to inhibit the uropathogenic strains of Escherichia coli. E. coli ATCC 25922 was used to characterize the IVCM (new in vitro catheterization model). As support for reducing bacterial proliferation, the cytotoxicity against a strain of Candida albicans was also determined (over 75% at 1 mg/mL). The results were correlated with the analysis of the distribution of biologically active compounds (trans-ferulic acid-268.44 ± 0.001 mg/100 g extract and an equal quantity of Trans-p-coumaric acid and rosmarinic acid). A pronounced inhibitory effect against the uropathogenic strain E. coli 317 (4 log copy no./mL after 72 h) was determined. The results showed a targeted response to the product for tested bacterial strains. The importance of research resulted from the easy and fast characterization of the functional product with antimicrobial effect against uropathogenic strains of E. coli. This study demonstrated that the proposed in vitro model was a valuable tool for assessing urinary tract infections with E. coli.

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The Use of a Micro Near Infrared Portable Instrument to Predict Bioactive Compounds in a Wild Harvested Fruit—Kakadu Plum (Terminalia ferdinandiana)

Sensors ◽

10.3390/s21041413 ◽

2021 ◽

Vol 21 (4) ◽

pp. 1413

Author(s):

Eshetu Bobasa ◽

Anh Dao T. Phan ◽

Michael Netzel ◽

Heather E. Smyth ◽

Yasmina Sultanbawa ◽

...

Keyword(s):

Vitamin C ◽

Bioactive Compounds ◽

Ellagic Acid ◽

Near Infrared ◽

Wide Range ◽

Fruit Samples ◽

Nutritional Characteristics ◽

Analytical Quantification ◽

Kakadu Plum ◽

Terminalia Ferdinandiana

Kakadu plum (KP; Terminalia ferdinandiana Exell, Combretaceae) is an emergent indigenous fruit originating from Northern Australia, with valuable health and nutritional characteristics and properties (e.g., high levels of vitamin C and ellagic acid). In recent years, the utilization of handheld NIR instruments has allowed for the in situ quantification of a wide range of bioactive compounds in fruit and vegetables. The objective of this study was to evaluate the ability of a handheld NIR spectrophotometer to measure vitamin C and ellagic acid in wild harvested KP fruit samples. Whole and pureed fruit samples were collected from two locations in the Kimberley region (Western Australia, Australia) and were analysed using both reference and NIR methods. The standard error in cross validation (SECV) and the residual predictive deviation (RPD) values were 1.81% dry matter (DM) with an RPD of 2.1, and 3.8 mg g−1 DM with an RPD of 1.9 for the prediction of vitamin C and ellagic acid, respectively, in whole KP fruit. The SECV and RPD values were 1.73% DM with an RPD of 2.2, and 5.6 mg g−1 DM with an RPD of 1.3 for the prediction of vitamin C and ellagic acid, respectively, in powdered KP samples. The results of this study demonstrated the ability of a handheld NIR instrument to predict vitamin C and ellagic acid in whole and pureed KP fruit samples. Although the RPD values obtained were not considered adequate to quantify these bioactive compounds (e.g., analytical quantification), this technique can be used as a rapid tool to screen vitamin C in KP fruit samples for high and low quality vitamin C.

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