scholarly journals Three-Dimensional (3D) Fibronectin Nano-Array Presented on Fibrin Matrix Accelerates Mice Skin Wound Healing

2020 ◽  
Author(s):  
Carlos Poblete Jara ◽  
Ou Wang ◽  
Thais Paulino do Prado ◽  
Ayman Ismail ◽  
Frank Marco Fabian ◽  
...  
Keyword(s):  
Wound Healing ◽  
Umbilical Vein ◽  
Human Fibroblasts ◽  
Three Dimensional ◽  
Fibrin Clot ◽  
Skin Wound ◽  
Molar Ratio ◽  
Skin Wound Healing ◽  
Fibrin Matrix ◽  
Highly Active

AbstractPlasma fibrinogen (F1) and fibronectin (pFN) polymerize to form a fibrin clot that is both a hemostatic and provisional matrix for wound healing. About 90% of plasma F1 has a homodimeric pair of γ chains (γγF1) and 10% has a heterodimeric pair of γ and more acidic γ’ chains (γγ’F1). We have synthesized a novel fibrin matrix exclusively from a 1:1 (molar ratio) complex of γγ’F1 and pFN in the presence of highly active thrombin and recombinant Factor XIII (rFXIIIa). In this matrix, the fibrin nanofibers were wrapped with periodic 200-300 nm wide pFN nanobands (termed γγ’F1:pFN fibrin). In contrast, fibrin made from 1:1 mixture of γγF1 and pFN formed a sporadic distribution of “pFN droplets” (termed γγF1 +pFN fibrin). The γγ’F1:pFN fibrin enhanced the adhesion of primary human umbilical vein endothelium cells (HUVECs) relative to the γγF1+FN fibrin. Three dimensional (3D) culturing showed that the γγ’F1:pFN complex fibrin matrix enhanced the proliferation of both HUVECs and primary human fibroblasts. HUVECs in the 3D γγ’F1:pFN fibrin exhibited a starkly enhanced vascular morphogenesis while an apoptotic growth profile was observed in the γγF1 +pFN fibrin. Relative to γγF1 +pFN fibrin, mouse dermal wounds that were sealed by γγ’F1:pFN fibrin exhibited accelerated and enhanced healing. This study suggests that a 3D pFN nano-array presented on a fibrin matrix can promote wound healing.

scholarly journals Enzyme-Crosslinked Gelatin Hydrogel with Adipose-Derived Stem Cell Spheroid Facilitating Wound Repair in the Murine Burn Model

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2997
Author(s):  
Ting-Yu Lu ◽  
Kai-Fu Yu ◽  
Shuo-Hsiu Kuo ◽  
Nai-Chen Cheng ◽  
Er-Yuan Chuang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Three Dimensional ◽  
Stromal Vascular Fraction ◽  
Skin Wound ◽  
3D Scaffold ◽  
Skin Wound Healing ◽  
Cell Spheroid ◽  
Cell Spheroids ◽  
Adipose Derived Stem Cell

Engineered skin that can facilitate tissue repair has been a great advance in the field of wound healing. A well-designed dressing material together with active biological cues such as cells or growth factors can overcome the limitation of using auto-grafts from patients. Recently, many studies showed that human adipose-derived stem cells (hASCs) can be used to promote wound healing and skin tissue engineering. hASCs have already been widely applied for clinical trials. hASCs can be harvested abundantly because they can be easily isolated from fat tissue known as the stromal vascular fraction (SVF). On the other hand, increasing studies have proven that cells from spheroids can better simulate the biological microenvironment and can enhance the expression of stemness markers. However, a three-dimensional (3D) scaffold that can harbor implanted cells and can serve as a skin-repaired substitute still suffers from deficiency. In this study, we applied a gelatin/microbial transglutaminase (mTG) hydrogel to encapsulate hASC spheroids to evaluate the performance of 3D cells on skin wound healing. The results showed that the hydrogel is not toxic to the wound and that cell spheroids have significantly improved wound healing compared to cell suspension encapsulated in the hydrogel. Additionally, a hydrogel with cell spheroids was much more effective than other groups in angiogenesis since the cell spheroid has the possibility of cell–cell signaling to promote vascular generation.

scholarly journals Plasma Fibrin Clot Properties as Determinants of Bleeding Time in Human Subjects: Association with Histidine-Rich Glycoprotein

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Konstanty Szułdrzyński ◽  
Miłosz Jankowski ◽  
Daniel P. Potaczek ◽  
Anetta Undas
Keyword(s):  
Wound Healing ◽  
Bleeding Time ◽  
Human Subjects ◽  
Fibrin Clot ◽  
Skin Wound ◽  
Clot Lysis ◽  
Skin Wound Healing ◽  
Plasma Clot ◽  
Primary Hemostasis ◽  
Complex Relationships

Aims. Fibrin formation and histidine-rich glycoprotein (HRG) are involved in primary hemostasis and wound healing. Little is known regarding the relationship of clot characteristics, bleeding time, and wound healing. Methods and Results. We studied 154 patients with coronary artery disease (CAD) and 154 subjects free of CAD matched for age, obesity, and current smoking. We evaluated bleeding time (BT) using standardized skin incisions on a forearm, along with plasma clot permeability (Ks), clot lysis time (CLT), and histidine-rich glycoprotein (HRG). Compared with controls, BT was 45% shorter in CAD cases. CAD patients had 32% lower Ks and 17% longer CLT as well as 50% lower HRG compared with controls (all p<0.001). After adjusting for potential confounders, Ks and HRG levels were independent predictors of prolonged BT in CAD patients (OR 23.70, 95% CI 4.65-120.8 and OR 10.27, 95% CI 2.05-51.31, respectively) and controls (OR 10.89, 95% CI 2.31-51.11 and OR 4.54, 95% CI 1.07-19.27, respectively). Scar formation (n=79, 25.6%) was independently predicted by both short and prolonged BT in CAD cases (OR 21.87, 95% CI 7.41-64.55 and OR 10.17, 95% CI 2.88-35.97) and controls (OR 5.94, 95% CI 2.29-15.41 and OR 14.76, 95% CI 4.29-50.77, respectively). Conclusions. The study shows that plasma fibrin clot density and HRG may influence BT and that appropriate skin wound healing is associated with medium BT. Translational Perspective. Elucidation of the complex relationships between plasma fibrin clot phenotype and wound healing might have important practical implications.

Mesoglycan induces the secretion of microvesicles by keratinocytes able to activate human fibroblasts and endothelial cells: A novel mechanism in skin wound healing

2020 ◽  
Vol 869 ◽  
pp. 172894 ◽  
Author(s):  
Raffaella Belvedere ◽  
Emanuela Pessolano ◽  
Amalia Porta ◽  
Alessandra Tosco ◽  
Luca Parente ◽  
...  
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Human Fibroblasts ◽  
Skin Wound ◽  
Skin Wound Healing

Potential Beneficial Effects of Digitaria ciliaris Flower Absolute on the Wound Healing-Linked Activities of Fibroblasts and Keratinocytes

Planta Medica ◽  
2020 ◽  
Vol 86 (05) ◽  
pp. 348-355 ◽  
Author(s):  
Soo Min Park ◽  
Kyung Jong Won ◽  
Dae Il Hwang ◽  
Do Yoon Kim ◽  
Ha Bin Kim ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Fibroblasts ◽  
Skin Wound ◽  
Skin Regeneration ◽  
Mitogen Activated Protein Kinase ◽  
Boyden Chamber ◽  
Type I ◽  
Skin Wound Healing ◽  
Beneficial Effects ◽  
Ms Analysis

Abstract Digitaria ciliaris is widely reported to be a problematic weed in agricultural areas and is mainly used as an indicator plant for the development of herbicides. However, its bioactivities on skin regeneration and wound healing have not been investigated. In the present study, we investigated the effects of D. ciliaris flower absolute on skin wound healing and skin regeneration-related events, that is, proliferation, migration, and collagen biosynthesis, in human fibroblasts and keratinocytes. For this study, we extracted absolute from the D. ciliaris flower by solvent extraction and identified the composition of D. ciliaris flower absolute using GC/MS analysis. We also tested the effect of D. ciliaris flower absolute in CCD986sk fibroblasts and/or HaCaT keratinocytes using the WST assay and 5-bromo-2′-deoxyuridine incorporation assay, Boyden chamber assay, ELISA, sprouting assay, and immunoblotting. GC/MS analysis of D. ciliaris flower absolute revealed that it contained 15 compounds. The absolute increased the proliferations of keratinocytes and fibroblasts and the migration of fibroblasts but did not affect cell viabilities. In addition, it enhanced the syntheses of type I and IV collagen in fibroblasts, but not in keratinocytes. The sprouting assay showed increased sprout outgrowth of fibroblasts. In addition, D. ciliaris flower absolute induced the phosphorylation of extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase in fibroblasts. These results indicate that D. ciliaris flower absolute may promote skin wound healing/regeneration by inducing the proliferation, migration, and collagen synthesis of fibroblasts, as well as the proliferation of keratinocytes. Therefore, D. ciliaris flower absolute may be a potential natural source for cosmetic or pharmaceutical agents that promote skin wound healing/regeneration.

scholarly journals Skin Wound-Healing Potential of Polysaccharides from Medicinal Mushroom Auricularia auricula-judae (Bull.)

2021 ◽  
Vol 7 (4) ◽  
pp. 247
Author(s):  
Sariya Mapoung ◽  
Sonthaya Umsumarng ◽  
Warathit Semmarath ◽  
Punnida Arjsri ◽  
Pilaiporn Thippraphan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Fibroblasts ◽  
Healing Process ◽  
Skin Wound ◽  
Water Soluble ◽  
Wound Management ◽  
Migration And Invasion ◽  
Wound Healing Process ◽  
Skin Wound Healing ◽  
Auricularia Auricula

Auricularia auricula-judae, a nutrient-rich mushroom used in traditional medicine, is a macrofungi that exhibits various biological properties. In this study, we have reported on the mechanisms that promote the wound-healing effects of a water-soluble polysaccharide-rich extract obtained from A. auricula-judae (AAP). AAP contained high amounts of polysaccharides (349.83 ± 5.00 mg/g extract) with a molecular weight of 158 kDa. The main sugar composition of AAP includes mannose, galactose, and glucose. AAP displayed antioxidant activity in vitro and was able to abort UVB-induced intracellular ROS production in human fibroblasts in cellulo. AAP significantly promoted both fibroblast and keratinocyte proliferation, migration, and invasion, along with augmentation of the wound-healing process by increasing collagen synthesis and decreasing E-cadherin expression (All p < 0.05). Specifically, the AAP significantly accelerated the wound closure in a mice skin wound-healing model on day 9 (2.5%AAP, p = 0.031 vs. control) and day 12 (1% and 2.5%AAP with p = 0.009 and p < 0.001 vs. control, respectively). Overall, our results indicate that the wound-healing activities of AAP can be applied in an AAP-based product for wound management.

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