scholarly journals A meta-analysis and experiment assessing phage-based FMDV vaccine

2020 ◽  
Author(s):  
Peng Wu ◽  
Ningning Yang ◽  
Yueli Wang ◽  
Mingguo Xu ◽  
Yunfeng Zhang ◽  
...  
Keyword(s):  
Meta Analysis ◽  
Foot And Mouth Disease ◽  
Fixed Effect Model ◽  
Mouth Disease ◽  
Control Group ◽  
Phage Group ◽  
Mouth Disease Virus ◽  
Significant Difference ◽  
Foot And Mouth ◽  
And Control

AbstractFoot-and-mouth disease (FMD) is a pathological disease caused by the foot- and-mouth disease virus (FMDV), which mainly affects cloven-hoofed animals. This study was conducted to a meta-analysis and experiment on the effect of bacteriophages used in the development of FMDV vaccines. A systematic search was conducted for the collection of the protection effect for the phage-based FMDV vaccine using sensitive search strategies. The extracted data were analyzed using Rev-Man 5.4 software. This experiment used the T7 phage to express the capsid protein VP1 of the OHM-02 strain, and the recombinant VP1 phage was termed OHM-T7. Antibodies and cytokines levels were assessed after immunizing BALB/C mice with OHM-T7. The results showed that a total of 115 articles were retrieved, and 4 of them met the inclusion criteria. There was no heterogeneity with I2 = 0%, 20% or 43%. We used a fixed-effect model for meta-analysis, and the results showed a protective effect on FMDV between the phage group and control group (P<0.01) and between FMDV group and control group (P<0.01). Furthermore, when the phage group was compared to the FMDV group, there was also no significant difference (P>0.05). After successfully obtained the ohm-t7 strain and immunized the mice, it could induce high levels of IFN-γ levels in mice with little effect on IL-4 levels. OHM-T7 could be used to detect antibodies produced by mice immunized with different FMDV antigens and produce high levels of anti-FMD antibodies. In summary, these results showed the potential of phage-based FMDV vaccines in FMDV prevention.

Activity of different serum enzymes and hormone profilein foot and mouth disease affected cattle

2016 ◽  
Author(s):  
J. Barkakati ◽  
S. Sarma ◽  
D.J. Kalita ◽  
J. Goswami ◽  
K. Sharma
Keyword(s):  
Serum Alkaline Phosphatase ◽  
Foot And Mouth Disease ◽  
Serum Enzymes ◽  
Mouth Disease ◽  
Control Group ◽  
Indigenous Cattle ◽  
Significant Difference ◽  
Foot And Mouth ◽  
And Control ◽  
Alt Activity

Present study was undertaken to elucidate the effect of Foot and Mouth Disease on the activity of different serum enzymes and hormone profile in indigenous cattle of Assam. The serum alkaline phosphatase (ALP) and alanine transaminase (ALT) activity did not show significant difference among the affected, recovered and control group. However, significant (P is less than 0.01) increase of aspartate transaminase (AST) activity was recorded in the affected group as compared to control. The level of T3 showed a significant (Pis less than 0.05) increase during FMD infection. T4 and cortisol hormones were also found to be increased significantly (Pis less than 0.01) in FMD affected than recovered and control group. From the present study, it can be concluded that during FMD, there is a significant increase of AST , T3, T4 and cortisol. However, no significant change was recorded in the activity of ALT and ALP during FMD infection and recovered group..

scholarly journals Effect of Foot-and-Mouth Disease Vaccination on Acute Phase Immune Response and Anovulation in Hanwoo (Bos taurus coreanae)

Vaccines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 419
Author(s):  
Daehyun Kim ◽  
Joonho Moon ◽  
Jaejung Ha ◽  
Doyoon Kim ◽  
Junkoo Yi
Keyword(s):  
Immune Response ◽  
Artificial Insemination ◽  
Bos Taurus ◽  
Foot And Mouth Disease ◽  
Test Group ◽  
Mouth Disease ◽  
Control Group ◽  
Amyloid A ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Vaccination against foot-and-mouth disease is the most common method for preventing the spread of the disease; the negative effects include miscarriage, early embryo death, lower milk production, and decreased growth of fattening cattle. Therefore, in this study, we analyze the side effects of vaccination by determining the acute immune response and ovulation rate after vaccinating cows for foot-and-mouth disease. The test axis was synchronized with ovulation using 100 Hanwoo (Bos taurus coreanae) cows from the Gyeongsangbuk-do Livestock Research Institute; only individuals with estrus confirmed by ovarian ultrasound were used for the test. All test axes were artificially inseminated 21 days after the previous estrus date. The control group was administered 0.9% normal saline, the negative control was injected intramuscularly with lipopolysaccharide (LPS; 0.5 µg/kg), and the test group was administered a foot-and-mouth disease virus vaccine (FMDV vaccine; bioaftogen, O and A serotypes, inactivated vaccine) 2, 9, and 16 days before artificial insemination. White blood cells and neutrophils increased significantly 1 day after vaccination, and body temperature in the rumen increased for 16 h after vaccination. Ovulation was detected 1 day after artificial fertilization by ovarian ultrasound. The ovulation rates were as follows: control 89%, LPS 60%, FMDV vaccine (−2 d) 50%, FMDV vaccine (−9 d) 75%, and FMDV vaccine (−16 d) 75%. In particular, the FMDV vaccine (−2 d) test group confirmed that ovulation was delayed for 4 days after artificial insemination. In addition, it was confirmed that it took 9 days after inoculation for the plasma contents of haptoglobin and serum amyloid A to recover to the normal range as the main acute immune response factors. The conception rate of the FMDV vaccine (−2 d) group was 20%, which was significantly lower than that of the other test groups.

Foot-and-mouth disease virus infection of sheep: implications for diagnosis and control

2002 ◽  
Vol 150 (23) ◽  
pp. 724-727 ◽  
Author(s):  
G. J. Hughes ◽  
V. Mioulet ◽  
R. P. Kitching ◽  
S. Alexandersen ◽  
A. I. Donaldson ◽  
...  
Keyword(s):  
Virus Infection ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
And Control

scholarly journals 183 EFFECT OF THE ACIDIC ORGANIC BUFFER MES ON BOVINE IN VITRO EMBRYO PRODUCTION

2005 ◽  
Vol 17 (2) ◽  
pp. 242 ◽  
Author(s):  
K. de Haas ◽  
I. Luther ◽  
D. Gerber
Keyword(s):  
Culture Medium ◽  
Foot And Mouth Disease ◽  
Low Ph ◽  
Mouth Disease ◽  
Control Group ◽  
Maximal Volume ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Transferred embryos carry the risk of being vehicles of organisms causing diseases. Currently, the risk of in vitro-produced (IVP) embryos is more difficult to assess than the risk of in vivo-derived embryos, since less research has been published on the former. Foot and mouth disease virus (FMDV) is extremely sensitive to a low pH and is likely to be destroyed if embryos are exposed to a low pH for a short time. 2-(N-Morphalino)-ethanesulfonic acid (MES); an organic buffer with pKa 6.1; Sigma, South Africa, M2933) as been shown to destroy FMDV at a rate of 90% per minute at pH 6 and at a rate of 90% per second at pH 5 (Acharya et al. 1990 Vet. Microbiol. 23, 21–34; Thomson “Foot-and-mouth disease,” in Infectious Diseases of Livestock with Special Reference to Southern Africa, ed. Coetzer JAW, Thomson GR, and Tustin RC, Oxford University Press, Cape Town, 825–852). The aim of this study was to test whether exposing bovine oocytes and IVP zygotes to the organic buffer MES, buffered at pH 5.5, is detrimental to the development of bovine IVP embryos. IVM, IVF, and IVC was carried out with 1367 oocytes as described earlier [Jooste et al. 2003 Theriogenology 59, 443]. Oocytes were divided into three groups: 484 were used as controls (no MES exposure); 437 were in a maximal exposure group (MAX), i.e. MES treatment after washing of oocytes, after IVM and after IVF, and 446 had a minimal exposure (MIN), i.e. MES treatment after IVF only. To treat the oocytes with MES, 100 oocytes (from ten droplets) were drawn into a pipette in a maximal volume of 100 μL, and placed in 3 mL of MES, swirled around for 10 s, drawn up again in a maximal volume of 100 μL, and placed in 3 mL of culture medium. Oocytes or zygotes were then washed five times in culture medium before being processed through IVM, IVF, or IVC depending on their stage. Exposure of oocytes to MES varied from 30 to 60 s (10 s swirling and a variable time thereafter to pick up). A chi-square test was used to test for differences in cleavage and Day 7 blastocyst yield between control and treatment groups (P < 0.05). Cleavage (70%; 340/484) and blastocyst yield (32%; 156/484) in the control group were not different from those in MIN (68%; 304/446, and 29%; 131/446, respectively), but were significantly higher than for MAX (57%; 249/437, and 18%; 79/437, respectively). In MAX the MES had a harsh effect on the cumulus cells, making them granular and clumpy in appearance. Oocytes treated in MES solution adhered to the bottom of the dish, which made their handling difficult. Exposure time in MES was therefore variable and longer than initially planned. It is concluded that bovine IVP embryos can be exposed to MES without detrimental effect. Treatment with MAX still resulted in blastocysts but it did not yield good numbers. In future trials, treated dishes should be used to prevent oocyte and zygote adherence. Further research is needed to test whether FMDV can be removed from bovine IVP embryos with the described method.

scholarly journals Serum paraoxonase activity and total sialic acid in sheep with foot and mouth disease

2018 ◽  
Vol 74 (3) ◽  
pp. 199-202
Author(s):  
Haci Ahmet Deveci ◽  
Abdulsamed Kükürt ◽  
Gökhan Nur ◽  
Merve Alpay ◽  
Oğuz Merhan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sialic Acid ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Infected Group ◽  
Control Group ◽  
Paraoxonase Activity ◽  
Total Sialic Acid ◽  
Mouth Disease Virus ◽  
Foot And Mouth

The aim of this study was to investigate paraoxonase (PON1) activity and total sialic acid to be measured for the first time in sheep infected naturally with foot and mouth disease, and their relationships with oxidative stress. A total of 30 Awassi sheep (aged between 2–4), which were healthy and infected with the foot and mouth disease virus (FMDV) were used in the study. Concentrations of paraoxonase activity (PON1), which is an important antioxidant against oxidative stress, high density lipoprotein (HDL) and total sialic acid (TSA), which has a critical role for immune system and is one of the significant indicators of cellular degeneration, were measured in serum samples drawn from animals. The total oxidant capacity (TOC) and total antioxidant capacity (TAC) were evaluated to determine the oxidative balance. It was observed that serum PON1 (P&lt;0.001) and HDL (P&lt;0.01) concentrations were significantly lower compared to the control group. TSA concentration was higher in the infected group (P&lt;0.001) compared to the healthy group. TOC was higher (P&lt;0.001) and TAC was lower (P&lt;0.01) in the infected group compared to the control group. Consequently, harmful effects of the infection of foot and mouth disease were shown as cause of oxidative cell damage and the high rate of sialic acid was produced depending on the degeneration during the infection. .

scholarly journals Review on Outbreak Dynamics, the Endemic Serotypes, and Diversified Topotypic Profiles of Foot and Mouth Disease Virus Isolates in Ethiopia from 2008 to 2018

Viruses ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1076 ◽  
Author(s):  
Ashenafi Kiros Wubshet ◽  
Junfei Dai ◽  
Qian Li ◽  
Jie Zhang
Keyword(s):  
Animal Health ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Surveillance Systems ◽  
Spatial And Temporal Patterns ◽  
National Veterinary Institute ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Control And Prevention ◽  
And Control

Foot and mouth disease (FMD) endemicity in Ethiopia’s livestock remains an ongoing cause for economic concern, with new topotypes still arising even in previously unaffected areas. FMD outbreaks occur every year almost throughout the country. Understanding the outbreak dynamics, endemic serotypes, and lineage profiles of FMD in this country is very critical in designing control and prevention programs. For this, detailed information on outbreak dynamics in Ethiopia needs to be understood clearly. In this article, therefore, we review the spatial and temporal patterns and dynamics of FMD outbreaks from 2008 to 2018. The circulating serotypes and the topotypic profiles of the virus are also discussed. FMD outbreak data were obtained from; reports of MoARD (Ministry of Agriculture and Rural Development)/MoLF (Ministry of livestock and Fishery, NVI (National Veterinary Institute), and NAHDIC (National Animal Health Diagnostic and Investigation Center); published articles; MSc works; PhD theses; and documents from international organizations. To effectively control and prevent FMD outbreaks, animal health agencies should focus on building surveillance systems that can quickly identify and control ongoing outbreaks and implement efficient preventive measures.

scholarly journals Persistent Infection of African Buffalo (Syncerus caffer) with Foot-and-Mouth Disease Virus: Limited Viral Evolution and No Evidence of Antibody Neutralization Escape

2019 ◽  
Vol 93 (15) ◽  
Author(s):  
Martí Cortey ◽  
Luca Ferretti ◽  
Eva Pérez-Martín ◽  
Fuquan Zhang ◽  
Lin-Mari de Klerk-Lorist ◽  
...  
Keyword(s):  
Disease Virus ◽  
Immune Escape ◽  
Foot And Mouth Disease ◽  
Germinal Centers ◽  
Mouth Disease ◽  
African Buffalo ◽  
Syncerus Caffer ◽  
Mouth Disease Virus ◽  
Significant Difference ◽  
Foot And Mouth

ABSTRACTAfrican buffaloes (Syncerus caffer) are the principal “carrier” hosts of foot-and-mouth disease virus (FMDV). Currently, the epithelia and lymphoid germinal centers of the oropharynx have been identified as sites for FMDV persistence. We carried out studies in FMDV SAT1 persistently infected buffaloes to characterize the diversity of viruses in oropharyngeal epithelia, germinal centers, probang samples (oropharyngeal scrapings), and tonsil swabs to determine if sufficient virus variation is generated during persistence for immune escape. Most sequencing reads of the VP1 coding region of the SAT1 virus inoculum clustered around 2 subpopulations differing by 22 single-nucleotide variants of intermediate frequency. Similarly, most sequences from oropharynx tissue clustered into two subpopulations, albeit with different proportions, depending on the day postinfection (dpi). There was a significant difference between the populations of viruses in the inoculum and in lymphoid tissue taken at 35 dpi. Thereafter, until 400 dpi, no significant variation was detected in the viral populations in samples from individual animals, germinal centers, and epithelial tissues. Deep sequencing of virus from probang or tonsil swab samples harvested prior to postmortem showed less within-sample variability of VP1 than that of tissue sample sequences analyzed at the same time. Importantly, there was no significant difference in the ability of sera collected between 14 and 400 dpi to neutralize the inoculum or viruses isolated at later time points in the study from the same animal. Therefore, based on this study, there is no evidence of escape from antibody neutralization contributing to FMDV persistent infection in African buffalo.IMPORTANCEFoot-and-mouth disease virus (FMDV) is a highly contagious virus of cloven-hoofed animals and is recognized as the most important constraint to international trade in animals and animal products. African buffaloes (Syncerus caffer) are efficient carriers of FMDV, and it has been proposed that new virus variants are produced in buffalo during the prolonged carriage after acute infection, which may spread to cause disease in livestock populations. Here, we show that despite an accumulation of low-frequency sequence variants over time, there is no evidence of significant antigenic variation leading to immune escape. Therefore, carrier buffalo are unlikely to be a major source of new virus variants.

scholarly journals The Causative Agent of FMD Disease

2021 ◽  
Author(s):  
Yaxin Wang ◽  
Meijun Liu
Keyword(s):  
Disease Virus ◽  
Acute Infection ◽  
Genome Structure ◽  
Foot And Mouth Disease ◽  
Animal Husbandry ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Icosahedral Virus ◽  
Foot And Mouth ◽  
And Control

Foot-and-mouth disease (FMD) is an acute infection of cloven-hoofed animals caused by foot-and-mouth disease virus (FMDV). It is one of the most serious infectious diseases affecting animal husbandry and a major impediment to international trade in livestock and their products. Foot-and-mouth disease virus (FMDV), a member of the Picornaviridae family of Aphthovirus, is an icosahedral virus without envelope, 25–30 nm in diameter, containing about 8.4 kb of positive-sense single-stranded RNA. The virus exists in seven different serotypes: A, O, C, Asia1, SAT1, SAT2, and SAT3, but a large number of subtypes have evolved in each serotype. This chapter reviews the genome, structure, serotype, and epidemiology of FMDV, which will help people to further explore the mechanism of the interaction between foot-and-mouth disease virus and host and provide reference for scientific prevention and control of FMDV.

scholarly journals Promising Multiple-Epitope Recombinant Vaccine against Foot-and-Mouth Disease Virus Type O in Swine

2010 ◽  
Vol 18 (1) ◽  
pp. 143-149 ◽  
Author(s):  
Jun-Jun Shao ◽  
Chung Kai Wong ◽  
Tong Lin ◽  
Shuk Kwan Lee ◽  
Guo-Zheng Cong ◽  
...  
Keyword(s):  
Animal Health ◽  
Foot And Mouth Disease ◽  
Recombinant Vaccine ◽  
Mouth Disease ◽  
Inactivated Vaccine ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Heavy Chain Constant Region ◽  
World Organization ◽  
And Control

ABSTRACTIn order to develop a completely safe immunogen to replace the traditional inactivated vaccine, a tandem-repeat multiple-epitope recombinant vaccine against foot-and-mouth disease (FMD) virus (FMDV) type O was developed. It contained three copies each of residues 141 to 160 and 200 to 213 of VP1 of the O/China/99 strain of FMDV coupled with a swine immunoglobulin G heavy-chain constant region (scIgG). The data showed that the multiple-epitope recombinant vaccine elicited high titers of anti-FMDV specific antibodies in swine at 30 days postvaccination (dpv) and conferred complete protection against a challenge with 10350% swine infective doses of the O/China/99 strain. The anti-FMDV specific antibody titers were not significantly different between the multiple-epitope recombinant vaccine and the traditional vaccine (ttest,P> 0.05). The number of 50% pig protective doses was 6.47, which is higher than the number recommended by the World Organization for Animal Health. The multiple-epitope recombinant vaccine resulted in a duration of immunity of at least 6 months. We speculate that the multiple-epitope recombinant vaccine is a promising vaccine that may replace the traditional inactivated vaccine for the prevention and control of FMD in swine in the future.

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