scholarly journals Insect wing extract: A novel source for green synthesis of nanoparticles of antioxidant and antimicrobial potential

2020 ◽  
Author(s):  
Parameshwar Jakinala ◽  
Nageshwar Lingampally ◽  
Bee Hameeda ◽  
R. Z. Sayyed ◽  
M Yahya Khan ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Plasmon Band ◽  
Insect Wing ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Antibacterial And Antifungal Activities ◽  
Agricultural Potential ◽  
Antioxidant And Antimicrobial Activity ◽  
Gas Chromatography Mass Spectroscopy ◽  
Antibacterial And Antifungal

AbstractSilver nanoparticles (AgNPs) are among the most widely synthesized and used nanoparticles (NPs). AgNPs have been traditionally synthesized from plant extracts, cobwebs, microorganisms, etc. However, their synthesis from wing extracts of common insect; Mang mao which is abundantly available in most of the Asian countries has not been explored yet. We report the synthesis of AgNPs from M. mao wings extract and its antioxidant and antimicrobial activity. The synthesized AgNPs were spherical, 40–60 nm in size and revealed strong absorption plasmon band around at 430 nm. Highly crystalline nature of these particles as determined by Energy-dispersive X-ray analysis and X-ray diffraction further confirmed the presence of AgNPs. Hydrodynamic size and zeta potential of AgNPs were observed to be 43.9 nm and −7.12 mV, respectively. Fourier-transform infrared spectroscopy analysis revealed the presence of characteristic amide proteins and aromatic functional groups. Thin-layer chromatography (TLC) and Gas chromatography-mass spectroscopy (GC-MS) analysis revealed the presence of fatty acids in the wings extract that may be responsible for biosynthesis and stabilization of AgNPs. Further, SDS-PAGE of the insect wing extract protein showed the molecular weight of 49 kDa. M. mao silver nanoparticles (MMAgNPs) exhibit strong antioxidant, broad-range antibacterial and antifungal activities, which signifies their biomedical and agricultural potential.

scholarly journals Silver nanoparticles from insect wing extract: Biosynthesis and evaluation for antioxidant and antimicrobial potential

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0241729
Author(s):  
Parameshwar Jakinala ◽  
Nageshwar Lingampally ◽  
Bee Hameeda ◽  
R. Z. Sayyed ◽  
Yahya Khan M. ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Plasmon Band ◽  
Insect Wing ◽  
Antifungal Activities ◽  
X Ray ◽  
Antibacterial And Antifungal Activities ◽  
Agricultural Potential ◽  
Antioxidant And Antimicrobial Activity ◽  
Gas Chromatography Mass Spectroscopy ◽  
Antibacterial And Antifungal

Silver nanoparticles (AgNPs) are among the most widely synthesized and used nanoparticles (NPs). AgNPs have been traditionally synthesized from plant extracts, cobwebs, microorganisms, etc. However, their synthesis from wing extracts of common insect; Mang mao which is abundantly available in most of the Asian countries has not been explored yet. We report the synthesis of AgNPs from M. mao wings extract and its antioxidant and antimicrobial activity. The synthesized AgNPs were spherical, 40–60 nm in size and revealed strong absorption plasmon band around at 430 nm. Highly crystalline nature of these particles as determined by Energy-dispersive X-ray analysis and X-ray diffraction further confirmed the presence of AgNPs. Hydrodynamic size and zeta potential of AgNPs were observed to be 43.9 nm and -7.12 mV, respectively. Fourier-transform infrared spectroscopy analysis revealed the presence of characteristic amide proteins and aromatic functional groups. Thin-layer chromatography (TLC) and Gas chromatography-mass spectroscopy (GC-MS) analysis revealed the presence of fatty acids in the wings extract that may be responsible for biosynthesis and stabilization of AgNPs. Further, SDS-PAGE of the insect wing extract protein showed the molecular weight of 49 kDa. M. mao silver nanoparticles (MMAgNPs) exhibit strong antioxidant, broad-range antibacterial and antifungal activities, (66.8 to 87.0%), broad-range antibacterial and antifungal activities was found with maximum zone of inhibition against Staphylococcus aureus MTCC 96 (35±0.4 mm) and Fusarium oxysporum f. sp. ricini (86.6±0.4) which signifies their biomedical and agricultural potential.

scholarly journals Degradation of Methylene Blue Using Biologically Synthesized Silver Nanoparticles

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
M. Vanaja ◽  
K. Paulkumar ◽  
M. Baburaja ◽  
S. Rajeshkumar ◽  
G. Gnanajobitha ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Nitrate Solution ◽  
Reaction Medium ◽  
Silver Ions ◽  
Silver Nitrate Solution ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Aqueous Leaf Extract

Nowadays plant mediated synthesis of nanoparticles has great interest and achievement due to its eco-benign and low time consuming properties. In this study silver nanoparticles were successfully synthesized by usingMorinda tinctorialeaf extract under different pH. The aqueous leaf extract was added to silver nitrate solution; the color of the reaction medium was changed from pale yellow to brown and that indicates reduction of silver ions to silver nanoparticles. Thus synthesized silver nanoparticles were characterized by UV-Vis spectrophotometer. Dispersity and morphology was characterized by scanning electron microscope (SEM); crystalline nature and purity of synthesized silver nanoparticles were revealed by X-ray diffraction (XRD) and energy dispersive X-ray spectroscopy (EDX). FTIR spectrum was examined to identify the effective functional molecules responsible for the reduction and stabilization of silver nanoparticles synthesized by leaf extract. The photocatalytic activity of the synthesized silver nanoparticles was examined by degradation of methylene blue under sunlight irradiation. Green synthesized silver nanoparticles were effectively degrading the dye nearly 95% at 72 h of exposure time.

scholarly journals Green synthesis of Ag/TiO2 Nanocomposite Assisted by Gambier Leaf (Uncaria gambir Roxb) Extract

2019 ◽  
Vol 22 (6) ◽  
pp. 250-255 ◽  
Author(s):  
Sry Wahyuni ◽  
Syukri Syukri ◽  
Syukri Arief
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Diffuse Reflectance Spectroscopy ◽  
Irradiation Time ◽  
Rutile Phase ◽  
X Ray Diffraction ◽  
Silver Surface ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Vis Spectroscopy

Studies of green synthesis of nanoparticles mediated by plants extract is extensively explored and studied in recent time due to eco-friendliness, cost-effectiveness, and use a minimum amount of toxic chemicals in the process of inorganic material synthesis. In this study, the immobilization of silver nanoparticles on the surface of titanium dioxide (TiO2) was carried out using Uncaria gambier Roxb. leaf extract as a silver ion (Ag+) reducing agent. The synthesized Ag/TiO2 nanocomposite was characterized by UV-Vis spectroscopy, X-ray diffraction (XRD), and Diffuse Reflectance Spectroscopy (DRS). The formation of silver nanoparticles was confirmed through UV-Vis spectroscopic analysis, which showed a silver surface plasmon resonance (SPR) band at 426 nm. The X-ray diffraction pattern shows that Ag can inhibit the transition of the anatase into rutile phase. The presence of Ag particles in TiO2 can increase the absorption ability from an initial wavelength of 407 nm to 438 nm. Based on the results of Rhodamin B degradation, it can be seen that Ag/TiO2 has a higher photocatalytic activity than bare TiO2 with 99% percent degradation at 120 minutes of irradiation time.

Effect of Heating Time Duration on Synthesis of Colloidal Silver Nanoparticles

2016 ◽  
Vol 1141 ◽  
pp. 14-18 ◽  
Author(s):  
Kamakshi Patel ◽  
M.P. Deshpande ◽  
Vivek P. Gujarati ◽  
S.H. Chaki
Keyword(s):  
Silver Nanoparticles ◽  
Trisodium Citrate ◽  
Heating Time ◽  
Conventional Heating ◽  
Time Duration ◽  
Plasmon Band ◽  
X Ray ◽  
Size Controlled ◽  
Duration Of Heating ◽  
Distinct Increase

Size-controlled silver nanoparticles are prepared at two different heating time duration (30 and 60min) under conventional heating at 80 ̊C in an aqueous solution of silver nitrate (AgNO3) as a precursor and trisodium citrate (C6H5O7Na3.2H2O) as a reducing agent under continuous stirring. The size and size distribution of the resulting silver nanoparticles prepared under conventional heating are strongly dependent on the duration of heating. As the heating duration is increased, aggregation and grain growth is observed. When duration of heating was 60min a distinct increase in the particles size was observed that lead to shift in the plasmon band as confirmed by UV-Vis absorption spectroscopy. TEM images shows that silver nanoparticles are nearly spherical in shape and their sizes are ranging between 2-42 nm and their cubic structure was confirmed by X-ray diffractogram. From X-ray diffractogram we calculated crystallite size using Scherrer’s equation which comes out to about 36nm and that determined from Hall-Williamson plot turns out to be 19nm.

scholarly journals Nicotiana tabacum Mediated Green Synthesis of Silver Nanoparticles and Ag-Ni Nanohybrid: Optical and Antimicrobial Efficiency

2020 ◽  
Vol 21 (1) ◽  
pp. 179
Author(s):  
Anuoluwa Abimbola Akinsiku ◽  
Joseph Adeyemi Adekoya ◽  
Enock Olugbenga Dare
Keyword(s):  
Nicotiana Tabacum ◽  
Nanoparticle Synthesis ◽  
Phase Identification ◽  
Face Centered Cubic ◽  
Ag Nps ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Antimicrobial Efficiency ◽  
Visual Materials ◽  
Antibacterial And Antifungal

A sustainable method was considered for the preparation of nanosilver and its allied nanoparticles. Nicotiana tabacum, an abused plant, has found an application as a bio-chemical instead of lethal chemical in the synthesis of nanoparticles. As part of green chemistry implementation, double distilled water was the solvent used for extraction. The phytochemicals present were analyzed using standard procedures. Nanoparticle synthesis was carried out at varying precursor concentrations, and the reaction was monitored with a UV-visible spectrophotometer. Another optical characterization was also achieved with photoluminescence. Other characterization involved: X-ray diffraction (XRD), transmission electron microscopy (TEM), energy dispersive X-ray (EDS), and Fourier transform infrared spectroscopy techniques (FTIR). A matched phase identification of nanosilver resembled a face-centered cubic of Ag with a mean size of 11.68 nm, and a lattice constant of 4.0862 Å. The Ag NPs displayed activity against clinical isolates of fungi, Gram-positive, and Gram-negative bacteria as the growth inhibition was significant at P < 0.05. Ag NPs obtained from the Nicotiana tabacum proved to be an antibacterial and antifungal candidate, unlike the Ag NPs derived using chemical and physical methods, which were harmful for this purpose. Both the Ag NPs and Ag-Ni NPs displayed optical activity, which qualifies them for application in visual materials.

Green synthesis of chitosan capped silver nanoparticles and their antimicrobial activity

MRS Advances ◽  
2018 ◽  
Vol 3 (42-43) ◽  
pp. 2505-2517 ◽  
Author(s):  
Zondi Nate ◽  
Makwena Justice Moloto ◽  
Pierre Kalenga Mubiayi ◽  
Precious Nokwethemba Sibiya
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Spherical Particles ◽  
Cubic Phase ◽  
Face Centered Cubic ◽  
Surface Binding ◽  
Polymeric Compound ◽  
Antibacterial And Antifungal Activities ◽  
Bacteria And Fungi ◽  
Antibacterial And Antifungal

AbstractChitosan is a polymeric compound with functional groups which enable surface binding to nanoparticles and antibacterial activity. The antimicrobial activity was studied using silver nanoparticles with varied concentrations of chitosan. The nanoparticles were synthesized through a simple and environmentally friendly method at room temperature. Spherical particles with average sizes between 2 and 6 nm were obtained and their crystallinity showed a face-centered cubic phase. The evidence of chitosan presence on the nanoparticle surface was confirmed by the characteristic diffraction peak of chitosan and by FTIR spectra where the bonding of amine group could be depicted. The chitosan-capped silver nanoparticles showed good antibacterial and antifungal activities with MIC values between 0.20 and 1.5 mg.mL-1 compared to those obtained from most of references (up to 6.25 mg.mL-1) on the selected gram-positive (Staphylococcus aureus, Enterococcus faecalis), gram-negative (Klebsiella pneumoniae, Pseudomonas aeruginosa ) bacteria and fungi (Candida albicans, Cryptococcus neoformans).

scholarly journals Differential Action of Silver Nanoparticles on ABCB1 (MDR1) and ABCC1 (MRP1) Activity in Mammalian Cell Lines

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3383
Author(s):  
Damian Krzyzanowski ◽  
Marcin Kruszewski ◽  
Agnieszka Grzelak
Keyword(s):  
Silver Nanoparticles ◽  
Cell Lines ◽  
Atp Binding ◽  
Abc Proteins ◽  
Anticancer Properties ◽  
Antibacterial And Antifungal Activities ◽  
Mammalian Cell Lines ◽  
Nominal Size ◽  
Antibacterial And Antifungal ◽  
Atp Binding Cassette

Silver nanoparticles (AgNPs), due to their unique properties have been receiving immense attention in recent years. In addition to their antibacterial and antifungal activities, AgNPs also cause apoptosis, mitochondria disfunction, nucleic acid damage and show potent anticancer properties in both multidrug resistance (MDR) and sensitive tumors. The MDR phenomenon, caused by the presence of ATP-binding cassette (ABC) proteins, is responsible for the failure of chemotherapy. Thus, investigating the influence of widely used AgNPs on ABC transporters is crucial. In the present study, we have examined the cytotoxicity of silver nanoparticles of a nominal size of 20 nm (Ag20) on the cell lines of different tissue origins. In addition, we have checked the ATP-binding cassette transporters’ activity and expression under AgNP exposure. The results indicate that Ag20 shows a toxic effect on tested cells, as well as modulating the expression and transport activity of ABC proteins.

Synthesis of Silver Nanoparticles using Euphorbia wallichii Extract and Assessment of their Bio-functionalities

2020 ◽  
Vol 16 (4) ◽  
pp. 495-506 ◽  
Author(s):  
Abdul-Rehman Phull ◽  
Attarad Ali ◽  
Akhtar Ali ◽  
Sanaullah Abbasi ◽  
Muhammad Zia ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Brine Shrimp ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Cost Effective ◽  
Spectrometric Analysis ◽  
Bacterial Strains ◽  
X Ray ◽  
Synthesis Of Nanoparticles

Background: Silver nanoparticles synthesized by the bio-green method have been applied to various biomedical applications. These procedures are simple, eco-friendly and serve as an alternative to complex chemical methods for the preparation of nanomaterials. Objective: : In the present study, phytosynthesis of silver nanoparticles, to examine their antioxidant potential, toxic effects towards bacterial-, fungal-strains, brine shrimp nauplii and cancer cells was focused. Methods: Methanolic extract of Euphorbia wallichii roots was used for the synthesis of silver nanoparticles. The synthesis was monitored and confirmed by UV-visible spectroscopy, Fourier Transform Infra-Red (FTIR) spectrometric analysis, Field Emission Scanning Electron Microscope (FESEM), Energy Dispersive X-ray (EDX) and X-Ray Powder Diffraction (XRD). Results: The synthesized particles were average 63±8 nm in size. Involvement of phenolic (46.7±2.4 µg GAE/mg) and flavonoid (11.7±1.2 µg QE/mg) compounds as capping agents was also measured. Nanoparticles showed antioxidant properties in terms of free radical scavenging potential (59.63±1.0 %), reducing power (44.52±1.34 µg AAE/mg) and total antioxidant capacity (60.48±2.2 µg AAE/mg). The nanoparticles showed potent cytotoxic effects against brine shrimp nauplii (LD50 66.83 µg/ml), proliferation and cell death of HeLa cells as determined by MTT (LD50 0.3923 µg/ml) and TUNEL assays, respectively. Antimicrobial results revealed that silver nanoparticles were found to be more potent against pathogenic fungal (maximum active against A. fumigatus, MIC 15 µg/disc) and bacterial strains (maximum active against S. aureus, MIC 3.33 μg/disc) than the E. wallichii extract alone. Conclusion: These results support the advantages of using an eco-friendly and cost-effective method for synthesis of nanoparticles with antioxidant, cytotoxic and antimicrobial potential.

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