scholarly journals Visualising Cholesterol in Brain by On-Tissue Derivatisation and Quantitative Mass Spectrometry Imaging

2020 ◽  
Author(s):  
Roberto Angelini ◽  
Eylan Yutuc ◽  
Mark F Wyatt ◽  
Jillian Newton ◽  
Fowzi Adam Yusuf ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Brain Structures ◽  
Brain Regions ◽  
Null Mouse ◽  
Brain Health ◽  
Quantitative Mass Spectrometry ◽  
Tissue Sections ◽  
Niemann Pick ◽  
The Brain

SummaryDespite being a critical molecule for neurobiology and brain health, mass spectrometry imaging (MSI) of cholesterol has been under reported compared to other lipids, due to the difficulty in ionising the sterol molecule. In the present work we have employed an on-tissue enzyme-assisted derivatisation strategy to improve detection of cholesterol in brain tissue sections. We report distribution and levels of cholesterol across specific brain structures of the mouse brain, in a model of Niemann-Pick type C1 (NPC1) disease, and during brain development. MSI revealed how cholesterol changes during development and that in the adult is highest in pons and medulla of the brain stem. Cholesterol was significantly reduced in the corpus callosum and other brain regions in the Npc1 null mouse, confirming hypomyelination at the molecular level. Our study demonstrates the potential of MSI to the study of sterols in neuroscience.

Quantitative mass spectrometry imaging of small-molecule neurotransmitters in rat brain tissue sections using nanospray desorption electrospray ionization

The Analyst ◽  
2016 ◽  
Vol 141 (12) ◽  
pp. 3686-3695 ◽  
Author(s):  
Hilde-Marléne Bergman ◽  
Erik Lundin ◽  
Malin Andersson ◽  
Ingela Lanekoff
Keyword(s):  
Mass Spectrometry ◽  
Nervous System ◽  
Small Molecule ◽  
Mass Spectrometry Imaging ◽  
Quantitative Imaging ◽  
Desorption Electrospray Ionization ◽  
Quantitative Mass Spectrometry ◽  
Tissue Sections ◽  
System P ◽  
Rat Brain Tissue

Nano-DESI mass spectrometry imaging enables quantitative imaging of small-molecule neurotransmitters which are essential to the function of the nervous system.

scholarly journals Mass spectrometry imaging identifies abnormally elevated brain l-DOPA levels and extrastriatal monoaminergic dysregulation in l-DOPA–induced dyskinesia

2021 ◽  
Vol 7 (2) ◽  
pp. eabe5948
Author(s):  
Elva Fridjonsdottir ◽  
Reza Shariatgorji ◽  
Anna Nilsson ◽  
Theodosia Vallianatou ◽  
Luke R. Odell ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Side Effects ◽  
Mass Spectrometry Imaging ◽  
Brain Regions ◽  
Stria Terminalis ◽  
Brain Areas ◽  
Bed Nucleus ◽  
Neuronal Signaling ◽  
Cortical Areas ◽  
The Brain

l-DOPA treatment for Parkinson’s disease frequently leads to dyskinesias, the pathophysiology of which is poorly understood. We used MALDI-MSI to map the distribution of l-DOPA and monoaminergic pathways in brains of dyskinetic and nondyskinetic primates. We report elevated levels of l-DOPA, and its metabolite 3-O-methyldopa, in all measured brain regions of dyskinetic animals and increases in dopamine and metabolites in all regions analyzed except the striatum. In dyskinesia, dopamine levels correlated well with l-DOPA levels in extrastriatal regions, such as hippocampus, amygdala, bed nucleus of the stria terminalis, and cortical areas, but not in the striatum. Our results demonstrate that l-DOPA–induced dyskinesia is linked to a dysregulation of l-DOPA metabolism throughout the brain. The inability of extrastriatal brain areas to regulate the formation of dopamine during l-DOPA treatment introduces the potential of dopamine or even l-DOPA itself to modulate neuronal signaling widely across the brain, resulting in unwanted side effects.

scholarly journals Visualizing Cholesterol in the Brain by On-Tissue Derivatization and Quantitative Mass Spectrometry Imaging

2021 ◽  
Author(s):  
Roberto Angelini ◽  
Eylan Yutuc ◽  
Mark F. Wyatt ◽  
Jillian Newton ◽  
Fowzi A. Yusuf ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Quantitative Mass Spectrometry ◽  
The Brain

scholarly journals Quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

The Analyst ◽  
2018 ◽  
Vol 143 (3) ◽  
pp. 654-661 ◽  
Author(s):  
Milad Nazari ◽  
Mark T. Bokhart ◽  
Philip L. Loziuk ◽  
David C. Muddiman
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Mass Spectrometry Imaging ◽  
Ovarian Tissue ◽  
Desorption Electrospray Ionization ◽  
Laser Desorption ◽  
Quantitative Mass Spectrometry ◽  
Tissue Sections ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

IR-MALDESI quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissues.

scholarly journals Mass Spectrometry Imaging for Glycome in the Brain

2021 ◽  
Vol 15 ◽  
Author(s):  
Md. Mahmudul Hasan ◽  
Mst. Afsana Mimi ◽  
Md. Al Mamun ◽  
Ariful Islam ◽  
A. S. M. Waliullah ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Imaging Techniques ◽  
Enzymatic Digestion ◽  
Brain Regions ◽  
Enzymatic System ◽  
Biological Processes ◽  
Label Free ◽  
Post Translational Modification ◽  
The Brain

Glycans are diverse structured biomolecules that play crucial roles in various biological processes. Glycosylation, an enzymatic system through which various glycans are bound to proteins and lipids, is the most common and functionally crucial post-translational modification process. It is known to be associated with brain development, signal transduction, molecular trafficking, neurodegenerative disorders, psychopathologies, and brain cancers. Glycans in glycoproteins and glycolipids expressed in brain cells are involved in neuronal development, biological processes, and central nervous system maintenance. The composition and expression of glycans are known to change during those physiological processes. Therefore, imaging of glycans and the glycoconjugates in the brain regions has become a “hot” topic nowadays. Imaging techniques using lectins, antibodies, and chemical reporters are traditionally used for glycan detection. However, those techniques offer limited glycome detection. Mass spectrometry imaging (MSI) is an evolving field that combines mass spectrometry with histology allowing spatial and label-free visualization of molecules in the brain. In the last decades, several studies have employed MSI for glycome imaging in brain tissues. The current state of MSI uses on-tissue enzymatic digestion or chemical reaction to facilitate successful glycome imaging. Here, we reviewed the available literature that applied MSI techniques for glycome visualization and characterization in the brain. We also described the general methodologies for glycome MSI and discussed its potential use in the three-dimensional MSI in the brain.

scholarly journals Transcriptomic, peptidomic and mass spectrometry imaging analysis of the brain in the ant Cataglyphis nodus

2021 ◽  
Author(s):  
Jens Habenstein ◽  
Franziska Schmitt ◽  
Sander Liessem ◽  
Alice Ly ◽  
Dennis Trede ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Imaging Analysis ◽  
The Brain

scholarly journals Exemplifying the Screening Power of Mass Spectrometry Imaging over Label-Based Technologies for Simultaneous Monitoring of Drug and Metabolite Distributions in Tissue Sections

2015 ◽  
Vol 21 (2) ◽  
pp. 187-193 ◽  
Author(s):  
Richard J. A. Goodwin ◽  
Anna Nilsson ◽  
C. Logan Mackay ◽  
John G. Swales ◽  
Maria K. Johansson ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Cyclic Peptide ◽  
Analysis Data ◽  
Resolving Power ◽  
High Mass ◽  
Whole Body ◽  
Ion Cyclotron Resonance ◽  
Label Free ◽  
Tissue Sections

Mass spectrometry imaging (MSI) provides pharmaceutical researchers with a suite of technologies to screen and assess compound distributions and relative abundances directly from tissue sections and offer insight into drug discovery–applicable queries such as blood-brain barrier access, tumor penetration/retention, and compound toxicity related to drug retention in specific organs/cell types. Label-free MSI offers advantages over label-based assays, such as quantitative whole-body autoradiography (QWBA), in the ability to simultaneously differentiate and monitor both drug and drug metabolites. Such discrimination is not possible by label-based assays if a drug metabolite still contains the radiolabel. Here, we present data exemplifying the advantages of MSI analysis. Data of the distribution of AZD2820, a therapeutic cyclic peptide, are related to corresponding QWBA data. Distribution of AZD2820 and two metabolites is achieved by MSI, which [14C]AZD2820 QWBA fails to differentiate. Furthermore, the high mass-resolving power of Fourier transform ion cyclotron resonance MS is used to separate closely associated ions.

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