scholarly journals Analysis of Intermolt and Postmolt Transcriptomes Provides Insight into Molecular Mechanisms of the Red Swamp Crayfish, Procambarus Clarkii Molting

2020 ◽  
Author(s):  
Shengyan Su ◽  
Brian Pelekelo Munganga ◽  
Can Tian ◽  
JianLin Li ◽  
Fan Yu ◽  
...  
Keyword(s):  
Immune Response ◽  
Hormonal Regulation ◽  
Molecular Mechanisms ◽  
Procambarus Clarkii ◽  
Average Length ◽  
Energy Requirements ◽  
Red Swamp Crayfish ◽  
Molecular Events ◽  
Molting Process ◽  
Insight Into

AbstractIn the present study, we explored expression changes in the transcriptomes of two molting stages (post-molt (M) and Intermolt (NM)) of the red swamp crayfish. A total of 307608398 clean reads, with an average length of 145bp were obtained. Further clustering and assembly of the transcripts generated 139100 unigenes. The results were searched against the NCBI, NR, KEGG, Swissprot, and KOG databases, in order to annotate gene descriptions, associate them with gene ontology terms, and assign them to pathways. Several genes and other factors involved in a number of molecular events critical for molting, such as energy requirements, hormonal regulation, immune response, and exoskeleton formation were identified, evaluated, and characterized. The information presented here provides a basic understanding of the molecular mechanisms underlying the crayfish molting process, with respect to energy requirements, hormonal regulation, immune response, and skeletal related activities during post-molt stage and the intermolt stage.

scholarly journals Comparative Analysis of the Intermolt and Postmolt Hepatopancreas Transcriptomes Provides Insight into the Mechanisms of Procambarus clarkii Molting Process

Life ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 480
Author(s):  
Shengyan Su ◽  
Brian Pelekelo Munganga ◽  
Can Tian ◽  
JianLin Li ◽  
Fan Yu ◽  
...  
Keyword(s):  
Immune Response ◽  
Differentially Expressed Genes ◽  
Hormonal Regulation ◽  
De Novo ◽  
Procambarus Clarkii ◽  
Expression Profiles ◽  
R Package ◽  
Differentially Expressed ◽  
Rna Seq ◽  
Molting Process

In the present study, we used RNA-Seq to investigate the expression changes in the transcriptomes of two molting stages (postmolt (M) and intermolt (NM)) of the red swamp crayfish and identified differentially expressed genes. The transcriptomes of the two molting stages were de novo assembled into 139,100 unigenes with a mean length of 675.59 bp. The results were searched against the NCBI, NR, KEGG, Swissprot, and KOG databases, to annotate gene descriptions, associate them with gene ontology terms, and assign them to pathways. Furthermore, using the DESeq R package, differentially expressed genes were evaluated. The analysis revealed that 2347 genes were significantly (p > 0.05) differentially expressed in the two molting stages. Several genes and other factors involved in several molecular events critical for the molting process, such as energy requirements, hormonal regulation, immune response, and exoskeleton formation were identified and evaluated by correlation and KEGG analysis. The expression profiles of transcripts detected via RNA-Seq were validated by real-time PCR assay of eight genes. The information presented here provides a transient view of the hepatopancreas transcripts available in the postmolt and intermolt stage of crayfish, hormonal regulation, immune response, and skeletal-related activities during the postmolt stage and the intermolt stage.

scholarly journals Integrated Insight into the Molecular Mechanisms of Spontaneous Abortion during Early Pregnancy in Pigs

2021 ◽  
Vol 22 (12) ◽  
pp. 6644
Author(s):  
Xupeng Zang ◽  
Ting Gu ◽  
Wenjing Wang ◽  
Chen Zhou ◽  
Yue Ding ◽  
...  
Keyword(s):  
Immune Response ◽  
Spontaneous Abortion ◽  
Molecular Mechanisms ◽  
Gene Set Enrichment Analysis ◽  
Differentially Expressed ◽  
Gene Set ◽  
Cerna Network ◽  
Major Interest ◽  
End Stage ◽  
Insight Into

Due to the high rate of spontaneous abortion (SAB) in porcine pregnancy, there is a major interest and concern on commercial pig farming worldwide. Whereas the perturbed immune response at the maternal–fetal interface is an important mechanism associated with the spontaneous embryo loss in the early stages of implantation in porcine, data on the specific regulatory mechanism of the SAB at the end stage of the implantation remains scant. Therefore, we used high-throughput sequencing and bioinformatics tools to analyze the healthy and arresting endometrium on day 28 of pregnancy. We identified 639 differentially expressed lncRNAs (DELs) and 2357 differentially expressed genes (DEGs) at the end stage of implantation, and qRT-PCR was used to verify the sequencing data. Gene set variation analysis (GSVA), gene set enrichment analysis (GSEA), and immunohistochemistry analysis demonstrated weaker immune response activities in the arresting endometrium compared to the healthy one. Using the lasso regression analysis, we screened the DELs and constructed an immunological competitive endogenous RNA (ceRNA) network related to SAB, including 4 lncRNAs, 11 miRNAs, and 13 genes. In addition, Blast analysis showed the applicability of the constructed ceRNA network in different species, and subsequently determined HOXA-AS2 in pigs. Our study, for the first time, demonstrated that the SAB events at the end stages of implantation is associated with the regulation of immunobiological processes, and a specific molecular regulatory network was obtained. These novel findings may provide new insight into the possibility of increasing the litter size of sows, making pig breeding better and thus improving the efficiency of animal husbandry production.

The red swamp crayfish, Procambarus clarkii cathepsin C, participates in the innate immune response to the viral and bacterial pathogens

2020 ◽  
Vol 100 ◽  
pp. 436-444 ◽  
Author(s):  
Qiu-Ning Liu ◽  
Saima Kausar ◽  
Isma Gul ◽  
Hai-Ling Zhou ◽  
Muhammad Nadeem Abbas ◽  
...  
Keyword(s):  
Immune Response ◽  
Innate Immune Response ◽  
Procambarus Clarkii ◽  
Bacterial Pathogens ◽  
Innate Immune ◽  
Cathepsin C ◽  
Red Swamp Crayfish

scholarly journals Microarray analysis reveals the role of matrix metalloproteinases in mouse experimental autoimmune myocarditis induced by cardiac myosin peptides

2007 ◽  
Vol 12 (2) ◽  
Author(s):  
Qizhu Tang ◽  
Ji Huang ◽  
Haiyan Qian ◽  
Ran Xiong ◽  
Difei Shen ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Microarray Analysis ◽  
Molecular Mechanisms ◽  
Global Changes ◽  
Autoimmune Myocarditis ◽  
Molecular Events ◽  
Specific Antigens ◽  
Experimental Mouse ◽  
Insight Into

AbstractAutoimmune myocarditis develops after the presentation of heart-specific antigens to autoaggressive CD4+ T cells and after inflammation has infiltrated the tissues. To shed light on global changes in the gene expression of autoimmune myocarditis and to gain further insight into the molecular mechanisms underlying the genesis of myocarditis, we conducted a comprehensive microarray analysis of mRNA using an experimental mouse autoimmune myocarditis model via immunization with α-myosin heavy chain-derived peptides. Of over 39,000 transcripts on a high density oligonucleotide microarray, 466 were under-expressed and 241 over-expressed by ≥ 1.5-fold compared with the controls in BALB/C mouse with autoimmune myocarditis. In this paper, we list the top 50 up-regulated genes related to the immune response. These altered genes encode for leukocyte-specific markers and receptors, the histocompatibility complex, cytokines/receptors, chemokines/receptors, adhesion molecules, components of the complement cascade, and signal transduction-related molecules. Interestingly, matrix metalloproteinases (MMPs) such as MMP-3 and MMP-9 were up-regulated, as further revealed by the reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry assays. This indicates that MMPs may act as major regulators of the cytokine profile. Together, these findings provide new insight into the molecular events associated with the mechanism of the autoimmune genesis of myocarditis.

C-type lectin from red swamp crayfish Procambarus clarkii participates in cellular immune response

2011 ◽  
Vol 76 (3) ◽  
pp. 168-184 ◽  
Author(s):  
Xiao-Wen Zhang ◽  
Xian-Wei Wang ◽  
Chen Sun ◽  
Xiao-Fan Zhao ◽  
Jin-Xing Wang
Keyword(s):  
Immune Response ◽  
Cellular Immune Response ◽  
Procambarus Clarkii ◽  
Red Swamp Crayfish ◽  
Cellular Immune
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