scholarly journals Serum-free medium increases the replication rate of the avian coronavirus infectious bronchitis virus in chicken embryo kidney cells

2021 ◽  
Author(s):  
José A. Quinteros ◽  
Glenn F. Browning ◽  
Amir H. Noormohammadi ◽  
Mark A. Stevenson ◽  
Mauricio J. C. Coppo ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Infectious Bronchitis Virus ◽  
Chicken Embryo ◽  
Culture Medium ◽  
Culture Media ◽  
Cell Culture Medium ◽  
Cell Culture Media ◽  
Infectious Bronchitis ◽  
Porcine Epidemic Diarrhoea Virus

AbstractInfectious bronchitis virus (IBV), an avian coronavirus, can be isolated and cultured in tracheal organ cultures (TOCs), embryonated eggs and cell cultures. TOCs and embryonated eggs are commonly used for viral isolation but use of these is laborious and expensive. Cell cultures have been used only with IBV strains that have previously been adapted to grow under laboratory conditions, and not for primary isolation. Previous studies using the coronavirus porcine epidemic diarrhoea virus (PEDV) have suggested that foetal bovine serum (FBS), a common component of cell culture media, can inhibit the adsorption of coronaviruses onto the host cell membrane receptors. In the present study, the replication of IBV in primary chicken embryo kidney (CEK) cell cultures and the Leghorn hepatocellular carcinoma (LMH) cell line was examined using two different cell culture media, one containing FBS and the other containing yeast extract (YE). A reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assay was used to quantify viral RNA copies in cell lysates. The highest concentrations of viral genomes were observed when the cell culture medium did not contain FBS. Examination of the infectivity of virus grown in CEK cell cultures was examined by titration in embryonated chicken eggs, demonstrating that the cell lysate from CEK cell cultures in medium without FBS contained a higher median embryo infectious dose (EID50) than that from CEK cell cultures in medium containing FBS. These results suggest that improved replication of IBV in cell cultures can be achieved by the omission of FBS from the cell culture medium. This may enhance the potential for production of vaccines in cell culture and facilitate the isolation of emergent IBV strains in cell cultures.

scholarly journals Selectivity of direct plasma treatment and plasma-conditioned media in bone cancer cell lines

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Inès Hamouda ◽  
Cédric Labay ◽  
Uroš Cvelbar ◽  
Maria-Pau Ginebra ◽  
Cristina Canal
Keyword(s):  
Cell Culture ◽  
Plasma Treatment ◽  
Cancer Cell ◽  
Culture Medium ◽  
Culture Media ◽  
Bone Cancer ◽  
Reactive Species ◽  
Conditioned Media ◽  
Cell Culture Medium ◽  
Cell Culture Media

AbstractAtmospheric pressure plasma jets have been shown to impact several cancer cell lines, both in vitro and in vivo. These effects are based on the biochemistry of the reactive oxygen and nitrogen species generated by plasmas in physiological liquids, referred to as plasma-conditioned liquids. Plasma-conditioned media are efficient in the generation of reactive species, inducing selective cancer cell death. However, the concentration of reactive species generated by plasma in the cell culture media of different cell types can be highly variable, complicating the ability to draw precise conclusions due to the differential sensitivity of different cells to reactive species. Here, we compared the effects of direct and indirect plasma treatment on non-malignant bone cells (hOBs and hMSCs) and bone cancer cells (SaOs-2s and MG63s) by treating the cells directly or exposing them to previously treated cell culture medium. Biological effects were correlated with the concentrations of reactive species generated in the liquid. A linear increase in reactive species in the cell culture medium was observed with increased plasma treatment time independent of the volume treated. Values up to 700 µM for H2O2 and 140 µM of NO2− were attained in 2 mL after 15 min of plasma treatment in AdvDMEM cell culture media. Selectivity towards bone cancer cells was observed after both direct and indirect plasma treatments, leading to a decrease in bone cancer cell viability at 72 h to 30% for the longest plasma treatment times while maintaining the survival of non-malignant cells. Therefore, plasma-conditioned media may represent the basis for a potentially novel non-invasive technique for bone cancer therapy.

The biochemical effects of extracellular Zn2+and other metal ions are severely affected by their speciation in cell culture media

Metallomics ◽  
2015 ◽  
Vol 7 (1) ◽  
pp. 102-111 ◽  
Author(s):  
H. Haase ◽  
S. Hebel ◽  
G. Engelhardt ◽  
L. Rink
Keyword(s):  
Cell Culture ◽  
Bovine Serum Albumin ◽  
Culture Medium ◽  
Culture Media ◽  
Zinc Homeostasis ◽  
Cell Culture Medium ◽  
Cell Culture Media ◽  
Biochemical Effects ◽  
Cellular Environment

Differential speciation and lower zinc buffering by less bovine serum albumin (BSA) in cell culture medium lead to altered zinc homeostasis compared to the cellular environmentin vivo.

scholarly journals The Cell Culture Medium Affects Growth, Phenotype Expression and the Response to Selenium Cytotoxicity in A549 and HepG2 Cells

Antioxidants ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 130 ◽  
Author(s):  
Lisa Arodin Selenius ◽  
Marita Wallenberg Lundgren ◽  
Rim Jawad ◽  
Olof Danielsson ◽  
Mikael Björnstedt
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Culture Medium ◽  
Culture Media ◽  
A549 Cells ◽  
Culture Conditions ◽  
Cell Culture Medium ◽  
Cell Culture Media ◽  
Toxic Response ◽  
Selenium Compounds

Selenium compounds influence cell growth and are highly interesting candidate compounds for cancer chemotherapy. Over decades an extensive number of publications have reported highly efficient growth inhibitory effects with a number of suggested mechanisms f especially for redox-active selenium compounds. However, the studies are difficult to compare due to a high degree of variations in half-maximal inhibitor concentration (IC50) dependent on cultivation conditions and methods to assess cell viability. Among other factors, the variability in culture conditions may affect the experimental outcome. To address this, we have compared the maintenance effects of four commonly used cell culture media on two cell lines, A549 and HepG2, evaluated by the toxic response to selenite and seleno-methylselenocysteine, cell growth and redox homeostasis. We found that the composition of the cell culture media greatly affected cell growth and sensitivity to selenium cytotoxicity. We also provided evidence for change of phenotype in A549 cells when maintained under different culture conditions, demonstrated by changes in cytokeratin 18 (CK18) and vimentin expression. In conclusion, our results have shown the importance of defining the cell culture medium used when comparing results from different studies.

Studies on the renin-angiotensin system in primary monolayer cell cultures of the rat epididymis

1991 ◽  
Vol 131 (2) ◽  
pp. 287-293 ◽  
Author(s):  
P. Y. D. Wong ◽  
C. N. Uchendu
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Culture Medium ◽  
Renin Angiotensin System ◽  
Cell Culture Medium ◽  
Angiotensin System ◽  
Monolayer Cell ◽  
Renin Angiotensin ◽  
Cell Monolayers ◽  
Cell Lysate

ABSTRACT Monolayer cell cultures formed from the rat cauda epididymidis exhibited renin-like and angiotensin-converting enzyme (ACE) activities and contained immunoreactive angiotensin I (AI) and angiotensin II (AII). Renin-like activity, determined indirectly by radioimmunoassay of generated AI at a near-neutral pH of 6·0, was demonstrated in the cell lysate but was almost undetectable in the serum-free cell culture medium, suggesting that renin expression in epididymal cells is an intracellular phenomenon. In contrast, both AI and AII were detected in the cell lysate and cell culture medium. The level of AI was enhanced by pretreating the cells with the ACE inhibitor captopril (100 nmol/l). Incubating the cell monolayers with thoroughly washed sperm cells obtained from the intact cauda epididymides of rats increase (P < 0·01) the AII content of the cell culture medium, with a parallel decline (P < 0·01) in the AI concentration. However, adrenaline (0·23 μmol/l), which was found to stimulate electrogenic anion secretion by cell monolayers grown on pervious supports, was without effect on the renin-like activity or concentration of angiotensins. The ACE activity in cells was confirmed by its strong dependence on chloride ion and its susceptibility to inhibition by captopril (100 nmol/l). Enzyme activity was significantly (P < 0·005) higher in the culture medium than in the cell lysate and cell membrane fragments. Angiotensinogen, which is obligatory for an intrinsic renin-angiotensin system, is present in epididymal cells. Presumably, it is synthesized and processed in the cell cytosol by intracellular renin. These findings in cultured cells provide further evidence for a local renin–angiotensin system in epididymal tissue and suggest a possible role of endogenous AII in the regulation of epididymal function. Journal of Endocrinology (1991) 131, 287–293

Cell Culture Medium for Preserving Cytopathic Effects in Cell Cultures

1982 ◽  
Vol 13 (4) ◽  
pp. 244-245 ◽  
Author(s):  
Jaclyn Gurtler ◽  
Harold C. Ballew ◽  
Carol M. Preissner ◽  
Thomas F. Smith
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Cytopathic Effects

scholarly journals Corynebacterium diphtheriae Proteome Adaptation to Cell Culture Medium and Serum

Proteomes ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 14
Author(s):  
Jens Möller ◽  
Fatemeh Nosratabadi ◽  
Luca Musella ◽  
Jörg Hofmann ◽  
Andreas Burkovski
Keyword(s):  
Cell Culture ◽  
Growth Medium ◽  
Culture Medium ◽  
Culture Media ◽  
Calf Serum ◽  
Corynebacterium Diphtheriae ◽  
Protein Quantification ◽  
Cell Culture Medium ◽  
Animal Testing ◽  
Additional Advantage

Host-pathogen interactions are often studied in vitro using primary or immortal cell lines. This set-up avoids ethical problems of animal testing and has the additional advantage of lower costs. However, the influence of cell culture media on bacterial growth and metabolism is not considered or investigated in most cases. To address this question growth and proteome adaptation of Corynebacterium diphtheriae strain ISS3319 were investigated in this study. Bacteria were cultured in standard growth medium, cell culture medium, and fetal calf serum. Mass spectrometric analyses and label-free protein quantification hint at an increased bacterial pathogenicity when grown in cell culture medium as well as an influence of the growth medium on the cell envelope.

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