scholarly journals Systematic analysis of the R2R3-MYB family of transcription factors in Camellia sinensis: evidence for species-specific catechin biosynthesis regulation

2021 ◽  
Author(s):  
Jingyi Li ◽  
Shaoqun Liu ◽  
Peifen Chen ◽  
Jiarong Cai ◽  
Song Tang ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Transcription Factors ◽  
Camellia Sinensis ◽  
Myb Transcription Factor ◽  
Myb Transcription Factors ◽  
Evolutionary Diversification ◽  
Health Promoting ◽  
Myb Genes ◽  
Species Specific ◽  
R2r3 Myb

Tea from Camellia sinensis is one of the most popular beverages worldwide, lauded for its charming flavors and health-promoting properties. C. sinensis produces an abundance of specialized metabolites, which makes it an excellent model for digging into the genetic regulation of plant-specific metabolite biosynthesis. The most abundant health-promoting metabolites in tea are galloylated catechins, and the most bioactive of the galloylated catechins, epigallocatechin gallate (EGCG), is exclusively found in C. sinensis. The R2R3-MYB transcription factor family regulates metabolism of phenylpropanoids, the precursors to catechins, in various plant lineages. However, the transcriptional regulation of galloylated catechin biosynthesis remains elusive. Species-expanded or specific MYB transcription factors may regulate species-specific metabolite biosynthesis. This study mined the R2R3-MYB transcription factors associated with galloylated catechin biosynthesis in C. sinensis. A total of 118 R2R3-MYB proteins, classified into 38 subgroups, were identified. R2R3-MYB subgroups specific to or expanded in C. sinensis were hypothesized to be essential to evolutionary diversification of tea-specific metabolites. Notably, nine of these R2R3-MYB genes were expressed preferentially in apical buds and young leaves, exactly where galloylated catechins accumulate. Three putative R2R3-MYB genes displayed strong correlation with key galloylated catechin biosynthesis genes, suggesting a role in regulating biosynthesis of epicatechin gallate (ECG) and EGCG. Overall, this study paves the way to reveal the transcriptional regulation of galloylated catechins in C. sinensis.

scholarly journals Systematic Analysis of the R2R3-MYB Family in Camellia sinensis: Evidence for Galloylated Catechins Biosynthesis Regulation

2022 ◽  
Vol 12 ◽  
Author(s):  
Jingyi Li ◽  
Shaoqun Liu ◽  
Peifen Chen ◽  
Jiarong Cai ◽  
Song Tang ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Camellia Sinensis ◽  
Genetic Regulation ◽  
Epigallocatechin Gallate ◽  
Myb Transcription Factor ◽  
Epicatechin Gallate ◽  
Systematic Analysis ◽  
Specialized Metabolites ◽  
Evolutionary Diversification ◽  
R2r3 Myb

The R2R3-MYB transcription factor (TF) family regulates metabolism of phenylpropanoids in various plant lineages. Species-expanded or specific MYB TFs may regulate species-specific metabolite biosynthesis including phenylpropanoid-derived bioactive products. Camellia sinensis produces an abundance of specialized metabolites, which makes it an excellent model for digging into the genetic regulation of plant-specific metabolite biosynthesis. The most abundant health-promoting metabolites in tea are galloylated catechins, and the most bioactive of the galloylated catechins, epigallocatechin gallate (EGCG), is specifically relative abundant in C. sinensis. However, the transcriptional regulation of galloylated catechin biosynthesis remains elusive. This study mined the R2R3-MYB TFs associated with galloylated catechin biosynthesis in C. sinensis. A total of 118 R2R3-MYB proteins, classified into 38 subgroups, were identified. R2R3-MYB subgroups specific to or expanded in C. sinensis were hypothesized to be essential to evolutionary diversification of tea-specialized metabolites. Notably, nine of these R2R3-MYB genes were expressed preferentially in apical buds (ABs) and young leaves, exactly where galloylated catechins accumulate. Three putative R2R3-MYB genes displayed strong correlation with key galloylated catechin biosynthesis genes, suggesting a role in regulating biosynthesis of epicatechin gallate (ECG) and EGCG. Overall, this study paves the way to reveal the transcriptional regulation of galloylated catechins in C. sinensis.

scholarly journals Transcriptional regulation of flavonoid biosynthesis in nectarine (Prunus persica) by a set of R2R3 MYB transcription factors

2013 ◽  
Vol 13 (1) ◽  
pp. 68 ◽  
Author(s):  
Daniela Ravaglia ◽  
Richard V Espley ◽  
Rebecca A Henry-Kirk ◽  
Carlo Andreotti ◽  
Vanina Ziosi ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Transcription Factors ◽  
Prunus Persica ◽  
Flavonoid Biosynthesis ◽  
Myb Transcription Factors ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

scholarly journals Phylogenomic Analysis of R2R3 MYB Transcription Factors in Sorghum and their Role in Conditioning Biofuel Syndrome

2020 ◽  
Vol 21 (2) ◽  
pp. 138-154
Author(s):  
Vinay Singh ◽  
Neeraj Kumar ◽  
Anuj K. Dwivedi ◽  
Rita Sharma ◽  
Manoj K. Sharma
Keyword(s):  
Transcription Factors ◽  
Large Scale ◽  
Agronomic Traits ◽  
Expression Patterns ◽  
Biomass Composition ◽  
Phylogenomic Analysis ◽  
Myb Transcription Factors ◽  
Myb Genes ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

Background : Large scale cultivation of sorghum for food, feed, and biofuel requires concerted efforts for engineering multipurpose cultivars with optimised agronomic traits. Due to their vital role in regulating the biosynthesis of phenylpropanoid-derived compounds, biomass composition, biotic, and abiotic stress response, R2R3-MYB family transcription factors are ideal targets for improving environmental resilience and economic value of sorghum. Methods: We used diverse computational biology tools to survey the sorghum genome to identify R2R3-MYB transcription factors followed by their structural and phylogenomic analysis. We used inhouse generated as well as publicly available high throughput expression data to analyse the R2R3 expression patterns in various sorghum tissue types. Results: We have identified a total of 134 R2R3-MYB genes from sorghum and developed a framework to predict gene functions. Collating information from the physical location, duplication, structural analysis, orthologous sequences, phylogeny, and expression patterns revealed the role of duplications in clade-wise expansion of the R2R3-MYB family as well as intra-clade functional diversification. Using publicly available and in-house generated RNA sequencing data, we provide MYB candidates for conditioning biofuel syndrome by engineering phenylpropanoid biosynthesis and sugar signalling pathways in sorghum. Conclusion: The results presented here are pivotal to prioritize MYB genes for functional validation and optimize agronomic traits in sorghum.

scholarly journals Genome-wide analysis of R2R3-MYB transcription factors family in the autopolyploid Saccharum spontaneum: an exploration of dominance expression and stress response

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Yuan Yuan ◽  
Xiping Yang ◽  
Mengfan Feng ◽  
Hongyan Ding ◽  
Muhammad Tahir Khan ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Gene Family ◽  
Expression Profiling ◽  
Saccharum Spontaneum ◽  
Myb Transcription Factors ◽  
Genome Wide Analysis ◽  
Myb Gene ◽  
Genome Wide ◽  
Myb Genes ◽  
R2r3 Myb

Abstract Background Sugarcane (Saccharum) is the most critical sugar crop worldwide. As one of the most enriched transcription factor families in plants, MYB genes display a great potential to contribute to sugarcane improvement by trait modification. We have identified the sugarcane MYB gene family at a whole-genome level through systematic evolution analyses and expression profiling. R2R3-MYB is a large subfamily involved in many plant-specific processes. Results A total of 202 R2R3-MYB genes (356 alleles) were identified in the polyploid Saccharum spontaneum genomic sequence and classified into 15 subgroups by phylogenetic analysis. The sugarcane MYB family had more members by a comparative analysis in sorghum and significant advantages among most plants, especially grasses. Collinearity analysis revealed that 70% of the SsR2R3-MYB genes had experienced duplication events, logically suggesting the contributors to the MYB gene family expansion. Functional characterization was performed to identify 56 SsR2R3-MYB genes involved in various plant bioprocesses with expression profiling analysis on 60 RNA-seq databases. We identified 22 MYB genes specifically expressed in the stem, of which RT-qPCR validated MYB43, MYB53, MYB65, MYB78, and MYB99. Allelic expression dominance analysis implied the differential expression of alleles might be responsible for the high expression of MYB in the stem. MYB169, MYB181, MYB192 were identified as candidate C4 photosynthetic regulators by C4 expression pattern and robust circadian oscillations. Furthermore, stress expression analysis showed that MYB36, MYB48, MYB54, MYB61 actively responded to drought treatment; 19 and 10 MYB genes were involved in response to the sugarcane pokkah boeng and mosaic disease, respectively. Conclusions This is the first report on genome-wide analysis of the MYB gene family in sugarcane. SsMYBs probably played an essential role in stem development and the adaptation of various stress conditions. The results will provide detailed insights and rich resources to understand the functional diversity of MYB transcription factors and facilitate the breeding of essential traits in sugarcane.

scholarly journals Origins and Stepwise Expansion of R2R3-MYB Transcription Factors for the Terrestrial Adaptation of Plants

2020 ◽  
Vol 11 ◽  
Author(s):  
Xiaojun Chang ◽  
Shupeng Xie ◽  
Lanlan Wei ◽  
Zhaolian Lu ◽  
Zhong-Hua Chen ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Land Plants ◽  
Monophyletic Group ◽  
Myb Transcription Factors ◽  
Genes Encoding ◽  
Terrestrial Environments ◽  
Myb Genes ◽  
Myb Proteins ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

The R2R3-MYB transcription factors play critical roles in various processes in embryophytes (land plants). Here, we identified genes encoding R2R3-MYB proteins from rhodophytes, glaucophytes, Chromista, chlorophytes, charophytes, and embryophytes. We classified the R2R3-MYB genes into three subgroups (I, II, and III) based on their evolutionary history and gene structure. The subgroup I is the most ancient group that includes members from all plant lineages. The subgroup II was formed before the divergence of charophytes and embryophytes. The subgroup III genes form a monophyletic group and only comprise members from land plants with conserved exon–intron structure. Each subgroup was further divided into multiple clades. The subgroup I can be divided into I-A, I-B, I-C, and I-D. The I-A, I-B, and I-C are the most basal clades that have originated before the divergence of Archaeplastida. The I-D with the II and III subgroups form a monophyletic group, containing only green plants. The II and III subgroups form another monophyletic group with Streptophyta only. Once on land, the subgroup III genes have experienced two rounds of major expansions. The first round occurred before the origin of land plants, and the second round occurred after the divergence of land plants. Due to significant gene expansion, the subgroup III genes have become the predominant group of R2R3-MYBs in land plants. The highly unbalanced pattern of birth and death evolution of R2R3-MYB genes indicates their important roles in the successful adaptation and massive radiation of land plants to occupy a multitude of terrestrial environments.

scholarly journals Expression pattern analysis of three R2R3-MYB transcription factors for the production of anthocyanin in different vegetative stages of Arabidopsis leaves

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Yeonjong Koo ◽  
R. Scott Poethig
Keyword(s):  
Transcription Factors ◽  
Developmental Stages ◽  
Myb Transcription Factors ◽  
Expression Pattern Analysis ◽  
Myb Genes ◽  
R2r3 Myb ◽  
Post Transcriptional Regulation ◽  
Anthocyanin Production ◽  
R2r3 Myb Transcription Factors

AbstractAnthocyanin is a type of flavonoid that appears purple in plants. PAP1, PAP2, and MYB113 are the three major R2R3-MYB transcription factors that regulate flavonoid biosynthesis in Arabidopsis thaliana. In this study, we found that the three MYB genes regulate anthocyanin accumulation in different leaf stages. Under limited nutrient conditions, PAP1 and PAP2 genes were highly induced in juvenile leaves. Conversely, MYB113 was expressed mainly in adult leaves. In addition, we investigated the role of trans-acting siRNA4 (TAS4) in the post-transcriptional regulation of anthocyanin expression in Arabidopsis leaves. In plant growth, the inhibition of PAP1 and PAP2 gene expression by TAS4 was observed only in juvenile leaves, and MYB113 inhibition was observed in adult leaves. In conclusion, we found that transcription and transcript repression of the three MYB genes is differentially regulated by TAS4 in leaf developmental stages. Our results improve the understanding of the regulation of plant anthocyanin production under stress conditions.

scholarly journals Expression of MYB transcription factor gene ZmMYB59 affects seed germination in Nicotiana tabacum and Oryza sativa

2020 ◽  
Author(s):  
Kaihui Zhai ◽  
Guangwu Zhao ◽  
Hongye Jiang ◽  
Caixia Sun ◽  
Jingyu Ren
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Seed Germination ◽  
Transgenic Tobacco ◽  
Germination Rate ◽  
Myb Transcription Factor ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Myb Transcription Factors ◽  
Vigor Index

Abstract Background MYB transcription factors are involved in many biological processes, including metabolism, development and responses to biotic and abiotic stresses. In our previous work, a new MYB transcription factor gene, ZmMYB59 was induced by deep sowing and down-regulated during maize seed germination via Real-Time PCR. However, there are few reports on seed germination regulated by MYB proteins and the functions of ZmMYB59 remain unknown. Results In this study, to examine its functions, Agrobacterium -mediated transformation was exploited to generate ZmMYB59 transgenic tobacco and rice. In T 2 generation transgenic tobacco, germination rate, germination index, vigor index and hypocotyl length were significantly decreased by 25.0~50.9%, 34.5~54.4%, 57.5~88.3% and 21.9~31.2% compared to wild-type (WT) lines. In T 2 generation transgenic rice, germination rate, germination index, vigor index and mesocotyl length were notably reduced by 39.1~53.8%, 51.4~71.4%, 52.5~74.0% and 28.3~41.5%, respectively. On this basis, relative physiological indicators were determined. The activities of catalase, peroxidase, superoxide dismutase, ascorbate peroxidase and proline content of transgenic lines were significantly lower than those of WT, suggesting that ZmMYB59 reduced their antioxidant capacity. As well, ZmMYB59 expression extremely inhibited the synthesis of gibberellin A1 (GA 1 ) and cytokinin (CTK), and promoted the synthesis of abscisic acid (ABA) concurrently, which implied that seed germination was repressed by ZmMYB59 in hormone levels. Furthermore, cell length and cell number of hypocotyl/mesocotyl in transgenic plants were notably decreased. Conclusions Taken together, it proposed that ZmMYB59 plays a negative regulation during seed germination in tobacco and rice, which also contributes to illuminate the molecular mechanisms regulated by MYB transcription factors.

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