scholarly journals Graphene Oxide reinforced Agarose-Hydroxyapatite Bioprinted 3-D Scaffolds for Bone Regeneration

2021 ◽  
Author(s):  
Umakant Yadav
Keyword(s):  
Graphene Oxide ◽  
Cellular Differentiation ◽  
Morphological Changes ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cells ◽  
3D Bioprinting ◽  
Promising Strategy ◽  
Morphogenetic Protein ◽  
Enhanced Expression ◽  
3D Printed

Three-dimensional (3D) bioprinting is an emerging technology for fabricating cells, biomaterials and extracellular matrix (ECM) into customized shapes and patterns. Here, we report additive manufacturing to create a customized 3D bioactive constructs for regenerative medicine. We have attempted to emphasize the use of agarose and graphene oxide as a promising material for the conceptualization of bioink unpaid to its unique physicochemical properties. The 3D printed structure is able to regenerating bone tissues and regulates the cellular differentiation without any significant morphological changes. The presence of graphene oxide enhances the osteoinductive behavior of the developed scaffolds, which is further supplemented by encapsulating human mesenchymal stem cells (hMSCs) on the 3D printed scaffolds. A significant enhanced expression of early osteogenic markers like morphogenetic protein (BMP), Runx-2, collagen-1, osteopontin, osteocalcin as well as mineralized ECM are observed on agarose-hydroxyapatite and graphene oxide 3D printed scaffolds compared to agarose-hydroxyapatite 3D printed scaffolds. Thus, the outcomes of the developed 3D bioprinted scaffolds provide a promising strategy for development of personalized bone grafts for tissue regeneration.

scholarly journals Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment

2021 ◽  
Vol 7 (4) ◽  
pp. 444
Author(s):  
Pei Zhuang ◽  
Yi-Hua Chiang ◽  
Maria Serafim Fernanda ◽  
Mei He
Keyword(s):  
Tumor Microenvironment ◽  
Prediction Models ◽  
Three Dimensional ◽  
3D Culture ◽  
Building Blocks ◽  
3D Bioprinting ◽  
Multicellular Spheroids ◽  
Tumor Models ◽  
3D Printed

Cancer still ranks as a leading cause of mortality worldwide. Although considerable efforts have been dedicated to anticancer therapeutics, progress is still slow, partially due to the absence of robust prediction models. Multicellular tumor spheroids, as a major three-dimensional (3D) culture model exhibiting features of avascular tumors, gained great popularity in pathophysiological studies and high throughput drug screening. However, limited control over cellular and structural organization is still the key challenge in achieving in vivo like tissue microenvironment. 3D bioprinting has made great strides toward tissue/organ mimicry, due to its outstanding spatial control through combining both cells and materials, scalability, and reproducibility. Prospectively, harnessing the power from both 3D bioprinting and multicellular spheroids would likely generate more faithful tumor models and advance our understanding on the mechanism of tumor progression. In this review, the emerging concept on using spheroids as a building block in 3D bioprinting for tumor modeling is illustrated. We begin by describing the context of the tumor microenvironment, followed by an introduction of various methodologies for tumor spheroid formation, with their specific merits and drawbacks. Thereafter, we present an overview of existing 3D printed tumor models using spheroids as a focus. We provide a compilation of the contemporary literature sources and summarize the overall advancements in technology and possibilities of using spheroids as building blocks in 3D printed tissue modeling, with a particular emphasis on tumor models. Future outlooks about the wonderous advancements of integrated 3D spheroidal printing conclude this review.

Electrochemical Determination of Bisphenol A in Saliva by a Novel Three-Dimensional (3D) Printed Gold-Reduced Graphene Oxide (rGO) Composite Paste Electrode

2019 ◽  
Vol 52 (16) ◽  
pp. 2583-2606 ◽  
Author(s):  
Livia Alexandra Gugoasa ◽  
Raluca-Ioana Stefan-van Staden ◽  
Jacobus Frederick van Staden ◽  
Maria Coroș ◽  
Stela Pruneanu
Keyword(s):  
Graphene Oxide ◽  
Bisphenol A ◽  
Reduced Graphene Oxide ◽  
Three Dimensional ◽  
Electrochemical Determination ◽  
Reduced Graphene ◽  
3D Printed ◽  
Paste Electrode

The Fabrication of Tissue Engineering Scaffolds by Inkjet Printing Technology

2018 ◽  
Vol 934 ◽  
pp. 129-133 ◽  
Author(s):  
Chao Fan Lv ◽  
Li Ya Zhu ◽  
Jian Ping Shi ◽  
Zong An Li ◽  
Wen Lai Tang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Sodium Alginate ◽  
Inkjet Printing ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Tissue Engineering Scaffolds ◽  
Printing Technology ◽  
3D Printed ◽  
Inkjet Printing Technology ◽  
Alginate Scaffold

Three-dimensional (3D) printing has been playing an important role in diverse areas in medicine. In order to promote the development of tissue engineering, this study attempts to fabricate tissue engineering scaffolds using the inkjet printing technology. Sodium alginate, exhibiting similar properties to the native human extracellular matrix (ECM), was used as bioink. The jetted fluid of sodium alginate would be gelatinized when printed into the calcium chloride solution. The characteristics of the 3D-printed sodium alginate scaffold were systematically measured and analyzed. The results show that, the pore size, porosity and degradation property of these scaffolds could be well controlled. This study indicates the capability of 3D bioprinting technology for preparing tissue engineering scaffolds.

scholarly journals Dynamic 3D tissue architecture directs BMP morphogen signaling during Drosophila wing morphogenesis

2018 ◽  
Author(s):  
Jinghua Gui ◽  
Yunxian Huang ◽  
Martin Kracklauer ◽  
Daniel Toddie-Moore ◽  
Kenji Kikushima ◽  
...  
Keyword(s):  
Growth Factor ◽  
Morphological Changes ◽  
Three Dimensional ◽  
Bmp Signaling ◽  
Growth Factor Signaling ◽  
Extracellular Signaling ◽  
Morphogenetic Protein ◽  
Pupal Wing ◽  
3D Architecture ◽  
Wing Morphogenesis

SummaryAt the level of organ formation, tissue morphogenesis drives developmental processes in animals, often involving the rearrangement of two-dimensional (2D) structures into more complex three-dimensional (3D) tissues. These processes can be directed by growth factor signaling pathways. However, little is known about how such morphological changes affect the spatiotemporal distribution of growth factor signaling. Here, using the Drosophila pupal wing, we address how Decapentaplegic (Dpp) / Bone Morphogenetic Protein (BMP) signaling and 3D wing morphogenesis are coupled. Dpp, expressed in the longitudinal veins (LVs) of the pupal wing, initially diffuses laterally during the inflation stage to regulate cell proliferation. Dpp localization is then refined to the LVs within each epithelial plane, but with active interplanar signaling for vein patterning, as the two epithelia appose. Our data further suggest that the 3D architecture of the wing epithelia directs the spatial distribution of BMP signaling, revealing how 3D morphogenesis is an emergent property of the interactions between extracellular signaling and tissue shape changes.

scholarly journals Three-Dimensional Bioprinting and Its Potential in the Field of Articular Cartilage Regeneration

Cartilage ◽  
2016 ◽  
Vol 8 (4) ◽  
pp. 327-340 ◽  
Author(s):  
Vivian H. M. Mouser ◽  
Riccardo Levato ◽  
Lawrence J. Bonassar ◽  
Darryl D. D’Lima ◽  
Daniel A. Grande ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Three Dimensional ◽  
Cartilage Regeneration ◽  
Biologically Active ◽  
3D Bioprinting ◽  
Clinical Images ◽  
3D Printed ◽  
Current Article ◽  
Hybrid Printing

Three-dimensional (3D) bioprinting techniques can be used for the fabrication of personalized, regenerative constructs for tissue repair. The current article provides insight into the potential and opportunities of 3D bioprinting for the fabrication of cartilage regenerative constructs. Although 3D printing is already used in the orthopedic clinic, the shift toward 3D bioprinting has not yet occurred. We believe that this shift will provide an important step forward in the field of cartilage regeneration. Three-dimensional bioprinting techniques allow incorporation of cells and biological cues during the manufacturing process, to generate biologically active implants. The outer shape of the construct can be personalized based on clinical images of the patient’s defect. Additionally, by printing with multiple bio-inks, osteochondral or zonally organized constructs can be generated. Relevant mechanical properties can be obtained by hybrid printing with thermoplastic polymers and hydrogels, as well as by the incorporation of electrospun meshes in hydrogels. Finally, bioprinting techniques contribute to the automation of the implant production process, reducing the infection risk. To prompt the shift from nonliving implants toward living 3D bioprinted cartilage constructs in the clinic, some challenges need to be addressed. The bio-inks and required cartilage construct architecture need to be further optimized. The bio-ink and printing process need to meet the sterility requirements for implantation. Finally, standards are essential to ensure a reproducible quality of the 3D printed constructs. Once these challenges are addressed, 3D bioprinted living articular cartilage implants may find their way into daily clinical practice.

scholarly journals 3D Bioprinting Photo-Crosslinkable Hydrogels for Bone and Cartilage Repair

2021 ◽  
Vol 7 (3) ◽  
pp. 367
Author(s):  
Quanjing Mei ◽  
Jingdong Rao ◽  
Ho Pan Bei ◽  
Yaxiong Liu ◽  
Xin Zhao
Keyword(s):  
Three Dimensional ◽  
3D Bioprinting ◽  
Hydrogel Scaffolds ◽  
Cartilage Engineering ◽  
Promising Strategy ◽  
Cartilage Defect Repair ◽  
Defect Repair ◽  
Excellent Control ◽  
Degradation Properties ◽  
And Cartilage

Three-dimensional (3D) bioprinting has become a promising strategy for bone manufacturing, with excellent control over geometry and microarchitectures of the scaffolds. The bioprinting ink for bone and cartilage engineering has thus become the key to developing 3D constructs for bone and cartilage defect repair. Maintaining the balance of cellular viability, drugs or cytokines’ function, and mechanical integrity is critical for constructing 3D bone and/or cartilage scaffolds. Photo-crosslinkable hydrogel is one of the most promising materials in tissue engineering; it can respond to light and induce structural or morphological transition. The biocompatibility, easy fabrication, as well as controllable mechanical and degradation properties of photo-crosslinkable hydrogel can meet various requirements of the bone and cartilage scaffolds, which enable it to serve as an effective bio-ink for 3D bioprinting. Here, in this review, we first introduce commonly used photo-crosslinkable hydrogel materials and additives (such as nanomaterials, functional cells, and drugs/cytokine), and then discuss the applications of the 3D bioprinted photo-crosslinkable hydrogel scaffolds for bone and cartilage engineering. Finally, we conclude the review with future perspectives about the development of 3D bioprinting photo-crosslinkable hydrogels in bone and cartilage engineering.

scholarly journals The effect of neural cell integrated into 3D co-axial bioprinted BMMSC structures during osteogenesis

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Yi Zhang ◽  
Haiyan Chen ◽  
Xiaoyan Long ◽  
Tao Xu
Keyword(s):  
Bone Development ◽  
Three Dimensional ◽  
Neural Cell ◽  
Bone Morphogenetic Protein 2 ◽  
Neural Function ◽  
3D Bioprinting ◽  
Support Cell ◽  
Morphogenetic Protein ◽  
New Strategy

Abstract A three-dimensional (3D) bioprinting is a new strategy for fabricating 3D cell-laden constructs that mimic the structural and functional characteristics of various tissues and provides a similar architecture and microenvironment of the native tissue. However, there are few reported studies on the neural function properties of bioengineered bone autografts. Thus, this study was aimed at investigating the effects of neural cell integration into 3D bioprinted bone constructs. The bioprinted hydrogel constructs could maintain long-term cell survival, support cell growth for human bone marrow-derived mesenchymal stem cells (BMMSCs), reduce cell surface biomarkers of stemness, and enhance orthopedic differentiation with higher expression of osteogenesis-related genes, including osteopontin (OPN) and bone morphogenetic protein-2. More importantly, the bioprinted constructs with neural cell integration indicated higher OPN gene and secretory alkaline phosphatase levels. These results suggested that the innervation in bioprinted bone constructs can accelerate the differentiation and maturation of bone development and provide patients with an option for accelerated bone function restoration.

scholarly journals Influence of Crosslinking on the Mechanical Behavior of 3D Printed Alginate Scaffolds: Experimental and Numerical Approaches

2018 ◽  
Author(s):  
Saman Naghieh ◽  
Mohammad Reza Karamooz-Ravari ◽  
Md Sarker ◽  
Eva Karki ◽  
Xiongbiao Chen
Keyword(s):  
Mechanical Properties ◽  
Elastic Modulus ◽  
Mechanical Behavior ◽  
Three Dimensional ◽  
Decisive Role ◽  
Element Model ◽  
Tissue Scaffolds ◽  
3D Bioprinting ◽  
3D Printed ◽  
Numerical Approaches

Tissue scaffolds fabricated by three-dimensional (3D) bioprinting are attracting considerableattention for tissue engineering applications. Because the mechanical properties of hydrogelscaffolds should match the damaged tissue, changing various parameters during 3D bioprintinghas been studied to manipulate the mechanical behavior of the resulting scaffolds. Crosslinkingscaffolds using a cation solution (such as CaCl2) is also important for regulating the mechanicalproperties, but has not been well documented in the literature. Here, the effect of variedcrosslinking agent volume and crosslinking time on the mechanical behavior of 3D bioplottedalginate scaffolds was evaulated using both experimental and numerical methods. Compressiontests were used to measure the elastic modulus of each scaffold, then a finite element model wasdeveloped and a power model used to predict scaffold mechanical behavior. Results showed thatcrosslinking time and volume of crosslinker both play a decisive role in modulating the mechanicalproperties of 3D bioplotted scaffolds. Because mechanical properties of scaffolds can affect cellresponse, the findings of this study can be implemented to modulate the elastic modulus ofscaffolds according to the intended application.

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