scholarly journals The effect of neural cell integrated into 3D co-axial bioprinted BMMSC structures during osteogenesis

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Yi Zhang ◽  
Haiyan Chen ◽  
Xiaoyan Long ◽  
Tao Xu
Keyword(s):  
Bone Development ◽  
Three Dimensional ◽  
Neural Cell ◽  
Bone Morphogenetic Protein 2 ◽  
Neural Function ◽  
3D Bioprinting ◽  
Support Cell ◽  
Morphogenetic Protein ◽  
New Strategy

Abstract A three-dimensional (3D) bioprinting is a new strategy for fabricating 3D cell-laden constructs that mimic the structural and functional characteristics of various tissues and provides a similar architecture and microenvironment of the native tissue. However, there are few reported studies on the neural function properties of bioengineered bone autografts. Thus, this study was aimed at investigating the effects of neural cell integration into 3D bioprinted bone constructs. The bioprinted hydrogel constructs could maintain long-term cell survival, support cell growth for human bone marrow-derived mesenchymal stem cells (BMMSCs), reduce cell surface biomarkers of stemness, and enhance orthopedic differentiation with higher expression of osteogenesis-related genes, including osteopontin (OPN) and bone morphogenetic protein-2. More importantly, the bioprinted constructs with neural cell integration indicated higher OPN gene and secretory alkaline phosphatase levels. These results suggested that the innervation in bioprinted bone constructs can accelerate the differentiation and maturation of bone development and provide patients with an option for accelerated bone function restoration.

scholarly journals Bone Regeneration Using a Three-Dimensional Hexahedron Channeled BCP Block Combined with Bone Morphogenic Protein-2 in Rat Calvarial Defects

Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2435 ◽  
Author(s):  
So-Yeun Kim ◽  
Eun-Bin Bae ◽  
Jae-Woong Huh ◽  
Jong-Ju Ahn ◽  
Hyun-Young Bae ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Alveolar Bone ◽  
Three Dimensional ◽  
Bone Morphogenetic Protein 2 ◽  
Graft Material ◽  
Implant Placement ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein ◽  
Calvarial Defects ◽  
Rat Calvarial Defect

It is important to obtain sufficient bone mass before implant placement on alveolar bone, and synthetic bone such as biphasic calcium phosphate (BCP) has been studied to secure this. This study used a BCP block bone with a specific structure of the three-dimensional (3D) hexahedron channel and coating with recombinant human bone morphogenetic protein-2 (rhBMP-2) impregnated carboxymethyl cellulose (CMC) was used to examine the enhancement of bone regeneration of this biomaterial in rat calvarial defect. After the preparation of critical-size calvarial defects in fifteen rats, defects were divided into three groups and were implanted with the assigned specimen (n = 5): Boneplant (untreated 3D hexahedron channeled BCP block), Boneplant/CMC (3D hexahedron channeled BCP block coated with CMC), and Boneplant/CMC/BMP (3D hexahedron channeled BCP block coated with CMC containing rhBMP-2). After 4 weeks, the volumetric, histologic, and histometric analyses were conducted to measure the newly formed bone. Histologically, defects in the Boneplant/CMC/BMP group were almost completely filled with new bone compared to the Boneplant and Boneplant/CMC groups. The new bone volume (P < 0.05) and area (P < 0.001) in the Boneplant/CMC/BMP group (20.12% ± 2.17, 33.79% ± 3.66) were much greater than those in the Boneplant (10.77% ± 4.8, 16.48% ± 9.11) and Boneplant/CMC (10.72% ± 3.29, 16.57% ± 8.94) groups, respectively. In conclusion, the 3D hexahedron channeled BCP block adapted rhBMP-2 with carrier CMC showed high possibility as an effective bone graft material.

scholarly journals Graphene Oxide reinforced Agarose-Hydroxyapatite Bioprinted 3-D Scaffolds for Bone Regeneration

2021 ◽  
Author(s):  
Umakant Yadav
Keyword(s):  
Graphene Oxide ◽  
Cellular Differentiation ◽  
Morphological Changes ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cells ◽  
3D Bioprinting ◽  
Promising Strategy ◽  
Morphogenetic Protein ◽  
Enhanced Expression ◽  
3D Printed

Three-dimensional (3D) bioprinting is an emerging technology for fabricating cells, biomaterials and extracellular matrix (ECM) into customized shapes and patterns. Here, we report additive manufacturing to create a customized 3D bioactive constructs for regenerative medicine. We have attempted to emphasize the use of agarose and graphene oxide as a promising material for the conceptualization of bioink unpaid to its unique physicochemical properties. The 3D printed structure is able to regenerating bone tissues and regulates the cellular differentiation without any significant morphological changes. The presence of graphene oxide enhances the osteoinductive behavior of the developed scaffolds, which is further supplemented by encapsulating human mesenchymal stem cells (hMSCs) on the 3D printed scaffolds. A significant enhanced expression of early osteogenic markers like morphogenetic protein (BMP), Runx-2, collagen-1, osteopontin, osteocalcin as well as mineralized ECM are observed on agarose-hydroxyapatite and graphene oxide 3D printed scaffolds compared to agarose-hydroxyapatite 3D printed scaffolds. Thus, the outcomes of the developed 3D bioprinted scaffolds provide a promising strategy for development of personalized bone grafts for tissue regeneration.

scholarly journals Boosting Solar Steam Generation by Using AIE Photothermal Molecule-Doped 3D Nanofibrous Aerogel with Self-Pumping Water Function

2019 ◽  
Author(s):  
Haoxuan Li ◽  
Haifei Wen ◽  
Jie Li ◽  
Jiachang Huang ◽  
Dong Wang ◽  
...  
Keyword(s):  
Solar Energy ◽  
Evaporation Rate ◽  
Sewage Treatment ◽  
Three Dimensional ◽  
Water Shortage ◽  
Steam Generation ◽  
New Strategy ◽  
Solar Steam Generation ◽  
High Evaporation Rate

<p>Utilizing solar energy to generate clean water by interface solar steam generation is considered to be a promising strategy to address the challenge of <a></a><a>water shortage globally.</a> However, <a></a><a>high evaporation rate and long-term sustainability have rarely been achieved simultaneously</a>, due to salt accumulation, discontinuous water supply and insufficient photothermal conversion. Herein, we demonstrate that a three-dimensional nanofibrous aerogel (3D NA) with Janus layers enables floating on the surface water by hydrophobic layer and continues pumping water by hydrophilic layer and interconnected porous structure. <a>More notably, an </a><a></a><a>aggregation-induced emission (AIE)</a> photothermal molecule is doped into nanofibers for the first time, which was endowed with superior capacity of transferring solar energy into heat. Combining these unique benefits, the presented 3D NA exhibits extremely high evaporation rate (1.99 kg m<sup>-2</sup> h<sup>-1</sup>) and solar-to-vapor conversion efficiency (89%) under irradiation of 1 sun. Besides, there is no significant change in evaporation performance after 21 cycles in the case of seawater treatment, suggesting that the designed 3D NA possess sustainable stability and self-cleaning function to restrain salt deposition. With highly efficient evaporation rate and long-term sustainable solar steam generation, such 3D NA can offer new strategy for desalination and sewage treatment. </p>

A Novel Deproteinized Bovine Bone Scaffold Containing Chitosan Microspheres for Controlled Delivery of BMP-2

2014 ◽  
Vol 651-653 ◽  
pp. 187-190
Author(s):  
Qing Li ◽  
Gang Zhou ◽  
Xin Yu ◽  
Tong Wang ◽  
Zhi Hui Tang
Keyword(s):  
Three Dimensional ◽  
Biologically Active ◽  
Bone Morphogenetic Protein 2 ◽  
Burst Release ◽  
Bovine Bone ◽  
Morphogenetic Protein ◽  
Novel Strategy ◽  
Novel Drug ◽  
Deproteinized Bovine Bone

The bone substation grafts, such as hydroxyapatite, TCP, have been used in the clinical. But it is found that they are not capable of enhancing bone regeneration due to their poor osteoinductive compared with allograft and autograft. In order to increase the osteoinductive, bone morphogenetic protein 2 (BMP-2) has highlighted to incorporate into a three dimensional reservoir. The purpose of the present study was to develop a novel drug delivery system with the capability of controlled releasing BMP-2. In this study, BMP-2/Chitosan (CS) microspheres were succeeded loaded on the deproteinized bovine bone (DBB) scaffold. The release profile of BMP-2 study indicated an initial burst release followed by a sustained release. In vitro bioactivity assay revealed that the encapsulated growth factor was biologically active as evidenced by cell culture. Therefore, the novel strategy of this microspheres-scaffold system is potentially to utilize the bone tissue engineered.

Sign in / Sign up

Export Citation Format

Share Document