scholarly journals CalR: A Web-based Analysis Tool for Indirect Calorimetry Experiments

2017 ◽  
Author(s):  
Amir I. Mina ◽  
Raymond A. LeClair ◽  
Katherine B. LeClair ◽  
David E. Cohen ◽  
Louise Lantier ◽  
...  
Keyword(s):  
Energy Balance ◽  
Energy Expenditure ◽  
Indirect Calorimetry ◽  
Statistical Tests ◽  
General Linear Model ◽  
Analysis Tool ◽  
Analysis Software ◽  
Web Based ◽  
Standard Tool ◽  
Data Files

AbstractWe report a web-based tool for analysis of indirect calorimetry experiments which measure physiological energy balance. CalR easily imports raw data files, generates plots, and determines the most appropriate statistical tests for interpretation. Analysis with the general linear model (which includes ANOVA and ANCOVA) allows for flexibility to interpret experiments of obesity and thermogenesis. Users may also produce standardized output files of an experiment which can be shared and subsequently re-evaluated using CalR. This framework will provide the transparency necessary to enhance consistency and reproducibility in experiments of energy expenditure. CalR analysis software will greatly increase the speed and efficiency with which metabolic experiments can be organized, analyzed according to accepted norms, and reproduced—and will likely become a standard tool for the field. CalR is accessible at https://CalR.bwh.harvard.edu.Graphical Abstract

scholarly journals An evaluation of the IDEEA™ activity monitor for estimating energy expenditure

2012 ◽  
Vol 109 (1) ◽  
pp. 173-183 ◽  
Author(s):  
Stephen Whybrow ◽  
Patrick Ritz ◽  
Graham W. Horgan ◽  
R. James Stubbs
Keyword(s):  
Energy Balance ◽  
Energy Expenditure ◽  
Indirect Calorimetry ◽  
Activity Patterns ◽  
Activity Monitor ◽  
Whole Body ◽  
Similar Estimate ◽  
Free Living ◽  
Doubly Labelled Water ◽  
D 12

Objective estimates of activity patterns and energy expenditure (EE) are important for the measurement of energy balance. The Intelligent Device for Energy Expenditure and Activity (IDEEA) can estimate EE from the thirty-five postures and activities it can identify and record. The present study evaluated the IDEEA system's estimation of EE using whole-body indirect calorimetry over 24 h, and in free-living subjects using doubly-labelled water (DLW) over 14 d. EE was calculated from the IDEEA data using calibration values for RMR and EE while sitting and standing, both as estimated by the IDEEA system (IDEEAest) and measured by indirect calorimetry (IDEEAmeas). Subjects were seven females and seven males, mean age 38·1 and 39·7 years, mean BMI 25·2 and 26·2 kg/m2, respectively. The IDEEAest method produced a similar estimate of EE to the calorimeter (10·8 and 10·8 MJ, NS), while the IDEEAmeas method underestimated EE (9·9 MJ, P < 0·001). After removing data from static cycling, which the IDEEA was unable to identify as an activity, both the IDEEAest and IDEEAmeas methods overestimated EE compared to the calorimeter (9·9 MJ, P < 0·001; 9·1 MJ, P < 0·05 and 8·6 MJ, respectively). Similarly, the IDEEA system overestimated EE compared to DLW over 14 d; 12·7 MJ/d (P < 0·01), 11·5 MJ/d (P < 0·01) and 9·5 MJ/d for the IDEEAest, IDEEAmeas and DLW, respectively. The IDEEA system overestimated EE both in the controlled laboratory and free-living environments. Using measured EE values for RMR, sitting and standing reduced, but did not eliminate, the error in estimated EE.

CQPweb — combining power, flexibility and usability in a corpus analysis tool

2012 ◽  
Vol 17 (3) ◽  
pp. 380-409 ◽  
Author(s):  
Andrew Hardie
Keyword(s):  
Data Model ◽  
Ease Of Use ◽  
Corpus Analysis ◽  
Analysis Tool ◽  
Analysis Software ◽  
Web Based ◽  
Query System ◽  
Analysis System ◽  
High Degree ◽  
Very High

CQPweb is a new web-based corpus analysis system, intended to address the conflicting requirements for usability and power in corpus analysis software. To do this, its user interface emulates the BNCweb system. Like BNCweb, CQPweb is built on two separate query technologies: the IMS Open Corpus Workbench and the MySQL relational database. CQPweb’s main innovative feature is its flexibility; its more generalised data model makes it compatible with any corpus. The analysis options available in CQPweb include: concordancing; collocations; distribution tables and charts; frequency lists; and keywords or key tags. An evaluation of CQPweb against criteria earlier laid down for a future web-based corpus analysis tool suggests that it fulfils many, but not all, of the requirements foreseen for such a piece of software. Despite some limitations, in making a sophisticated query system accessible to untrained users, CQPweb combines ease of use, power and flexibility to a very high degree.

scholarly journals CalR: A Web-Based Analysis Tool for Indirect Calorimetry Experiments

2018 ◽  
Vol 28 (4) ◽  
pp. 656-666.e1 ◽  
Author(s):  
Amir I. Mina ◽  
Raymond A. LeClair ◽  
Katherine B. LeClair ◽  
David E. Cohen ◽  
Louise Lantier ◽  
...  
Keyword(s):  
Indirect Calorimetry ◽  
Analysis Tool ◽  
Web Based

scholarly journals Indirect calorimetry in laboratory mice and rats: principles, practical considerations, interpretation and perspectives

2012 ◽  
Vol 303 (5) ◽  
pp. R459-R476 ◽  
Author(s):  
Patrick C. Even ◽  
Nachiket A. Nadkarni
Keyword(s):  
Energy Balance ◽  
Food Intake ◽  
Energy Expenditure ◽  
Indirect Calorimetry ◽  
Very High Frequency ◽  
Metabolic Challenge ◽  
The Common ◽  
Free Feeding ◽  
Basal Energy Expenditure

In this article, we review some fundamentals of indirect calorimetry in mice and rats, and open the discussion on several debated aspects of the configuration and tuning of indirect calorimeters. On the particularly contested issue of adjustment of energy expenditure values for body size and body composition, we discuss several of the most used methods and their results when tested on a previously published set of data. We conclude that neither body weight (BW), exponents of BW, nor lean body mass (LBM) are sufficient. The best method involves fitting both LBM and fat mass (FM) as independent variables; for low sample sizes, the model LBM + 0.2 FM can be very effective. We also question the common calorimetry design that consists of measuring respiratory exchanges under free-feeding conditions in several cages simultaneously. This imposes large intervals between measures, and generally limits data analysis to mean 24 h or day-night values of energy expenditure. These are then generally compared with energy intake. However, we consider that, among other limitations, the measurements of V̇o2, V̇co2, and food intake are not precise enough to allow calculation of energy balance in the small 2–5% range that can induce significant long-term alterations of energy balance. In contrast, we suggest that it is necessary to work under conditions in which temperature is set at thermoneutrality, food intake totally controlled, activity precisely measured, and data acquisition performed at very high frequency to give access to the part of the respiratory exchanges that are due to activity. In these conditions, it is possible to quantify basal energy expenditure, energy expenditure associated with muscular work, and response to feeding or to any other metabolic challenge. This reveals defects in the control of energy metabolism that cannot be observed from measurements of total energy expenditure in free feeding individuals.

scholarly journals DNAproDB: an expanded database and web-based tool for structural analysis of DNA–protein complexes

2019 ◽  
Author(s):  
Jared M Sagendorf ◽  
Nicholas Markarian ◽  
Helen M Berman ◽  
Remo Rohs
Keyword(s):  
Structural Analysis ◽  
Protein Complexes ◽  
Structural Data ◽  
Data Bank ◽  
Structural Features ◽  
Analysis Tool ◽  
Web Based ◽  
Initial Release ◽  
Data Files ◽  
User Friendly

Abstract DNAproDB (https://dnaprodb.usc.edu) is a web-based database and structural analysis tool that offers a combination of data visualization, data processing and search functionality that improves the speed and ease with which researchers can analyze, access and visualize structural data of DNA–protein complexes. In this paper, we report significant improvements made to DNAproDB since its initial release. DNAproDB now supports any DNA secondary structure from typical B-form DNA to single-stranded DNA to G-quadruplexes. We have updated the structure of our data files to support complex DNA conformations, multiple DNA–protein complexes within a DNAproDB entry and model indexing for analysis of ensemble data. Support for chemically modified residues and nucleotides has been significantly improved along with the addition of new structural features, improved structural moiety assignment and use of more sequence-based annotations. We have redesigned our report pages and search forms to support these enhancements, and the DNAproDB website has been improved to be more responsive and user-friendly. DNAproDB is now integrated with the Nucleic Acid Database, and we have increased our coverage of available Protein Data Bank entries. Our database now contains 95% of all available DNA–protein complexes, making our tools for analysis of these structures accessible to a broad community.

Activity and energy expenditure

1990 ◽  
Vol 68 (1) ◽  
pp. 17-27 ◽  
Author(s):  
M. J. Dauncey
Keyword(s):  
Energy Balance ◽  
Environmental Factors ◽  
Energy Expenditure ◽  
Indirect Calorimetry ◽  
Individual Variability ◽  
Energy Requirements ◽  
Resting Metabolism ◽  
The Subject ◽  
Activity Energy ◽  
Minor Activity

The influence of small changes in activity on energy expenditure and hence on energy requirements and energy balance is assessed. Evidence from direct and indirect calorimetry suggests that differences in spontaneous minor activity could readily alter 24-h energy expenditure by as much as 20%. This compares with values in the order of 10% for moderate overfeeding and somewhat less than this during mild cold exposure. Individual variability in 24-h energy expenditure can therefore be accounted for not only by differences in resting metabolism and the thermic responses to energy intake and temperature but also by differences in minor activity. Interactions between activity and environmental factors such as nutrition and temperature can modify the effect of activity on energy balance. Very little is known about mechanisms that could account for differences in spontaneous activity and these need to be the subject of future investigations.Key words: activity, energy balance, nutrition, temperature, thermogenesis.

scholarly journals CalR: A Web-based Analysis Tool for Indirect Calorimetry Experiments

2018 ◽  
Author(s):  
Amir I. Mina ◽  
Raymond A. LeClair ◽  
Katherine B. LeClair ◽  
David E. Cohen ◽  
Louise Lantier ◽  
...  
Keyword(s):  
Indirect Calorimetry ◽  
Analysis Tool ◽  
Web Based

scholarly journals The Diagnostic-Measurement Method—Resting Energy Expenditure Assessment of Polish Children Practicing Football

Diagnostics ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 340
Author(s):  
Edyta Łuszczki ◽  
Anna Bartosiewicz ◽  
Katarzyna Dereń ◽  
Maciej Kuchciak ◽  
Łukasz Oleksy ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Body Composition ◽  
Energy Expenditure ◽  
Muscle Mass ◽  
Indirect Calorimetry ◽  
Energy Demand ◽  
Body Height ◽  
Resting Energy Expenditure ◽  
Blood Pressure Monitor ◽  
Resting Energy

Establishing the amount of energy needed to cover the energy demand of children doing sport training and thus ensuring they achieve an even energy balance requires the resting energy expenditure (REE) to be estimated. One of the methods that measures REE is the indirect calorimetry method, which may be influenced by many factors, including body composition, gender, age, height or blood pressure. The aim of the study was to assess the correlation between the resting energy expenditure of children regularly playing football and selected factors that influence the REE in this group. The study was conducted among 219 children aged 9 to 17 using a calorimeter, a device used to assess body composition by the electrical bioimpedance method by means of segment analyzer and a blood pressure monitor. The results of REE obtained by indirect calorimetry were compared with the results calculated using the ready-to-use formula, the Harris Benedict formula. The results showed a significant correlation of girls’ resting energy expenditure with muscle mass and body height, while boys’ resting energy expenditure was correlated with muscle mass and body water content. The value of the REE was significantly higher (p ≤ 0.001) than the value of the basal metabolic rate calculated by means of Harris Benedict formula. The obtained results can be a worthwhile suggestion for specialists dealing with energy demand planning in children, especially among those who are physically active to achieve optimal sporting successes ensuring proper functioning of their body.

scholarly journals Effect of raw and encapsulated policosanol on lipid profiles, blood biochemistry, activity, energy expenditure and macronutrient metabolism of adult cats

2021 ◽  
pp. 1098612X2110137
Author(s):  
James R Templeman ◽  
Kylie Hogan ◽  
Alexandra Blanchard ◽  
Christopher PF Marinangeli ◽  
Alexandra Camara ◽  
...  
Keyword(s):  
Body Weight ◽  
Energy Expenditure ◽  
Indirect Calorimetry ◽  
Respiratory Quotient ◽  
Blood Count ◽  
Complete Blood Count ◽  
Serum Biochemistry ◽  
Blood Collection ◽  
Adult Cats ◽  
Activity Energy

Objectives The objective of this study was to verify the safety of policosanol supplementation for domestic cats. The effects of raw and encapsulated policosanol were compared with positive (L-carnitine) and negative (no supplementation) controls on outcomes of complete blood count, serum biochemistry, energy expenditure, respiratory quotient and physical activity in healthy young adult cats. Methods The study was a replicated 4 × 4 complete Latin square design. Eight cats (four castrated males, four spayed females; mean age 3.0 ± 1.0 years; mean weight 4.36 ± 1.08 kg; mean body condition score 5.4 ± 1.4) were blocked by sex and body weight then randomized to treatment groups: raw policosanol (10 mg/kg body weight), encapsulated policosanol (50 mg/kg body weight), L-carnitine (200 mg/kg body weight) or no supplementation. Treatments were supplemented to a basal diet for 28 days with a 1-week washout between periods. Food was distributed equally between two offerings to ensure complete supplement consumption (first offering) and measure consumption time (second offering). Blood collection (lipid profile, complete blood count, serum biochemistry) and indirect calorimetry (energy expenditure, respiratory quotient) were conducted at days 0, 14 and 28 of each period. Activity monitors were worn 7 days prior to indirect calorimetry and blood collection. Data were analyzed using a repeated measures mixed model (SAS, v.9.4). Results Food intake and body weight were similar among treatments. There was no effect of treatment on lipid profile, serum biochemistry, activity, energy expenditure or respiratory quotient ( P >0.05); however, time to consume a second meal was greatest in cats fed raw policosanol ( P <0.05). Conclusions and relevance These data suggest that policosanol is safe for feline consumption. Further studies with cats demonstrating cardiometabolic risk factors are warranted to confirm whether policosanol therapy is an efficacious treatment for hyperlipidemia and obesity.

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