scholarly journals Analysis of 19 Highly Conserved Vibrio cholerae Bacteriophages Isolated from Environmental and Patient Sources Over a Twelve-Year Period

2018 ◽  
Author(s):  
Angus Angermeyer ◽  
Moon Moon Das ◽  
Durg Vijai Singh ◽  
Kimberley D. Seed
Keyword(s):  
Vibrio Cholerae ◽  
Bay Of Bengal ◽  
Core Genome ◽  
Coastal Areas ◽  
Lytic Bacteriophage ◽  
Large Core ◽  
Accessory Genome ◽  
El Tor ◽  
Endemic Cholera ◽  
Over Time

AbstractThe Vibrio cholerae biotype ‘El Tor’ is responsible for all current epidemic and endemic cholera outbreaks worldwide. These outbreaks are clonal and are hypothesized to originate from the coastal areas near the Bay of Bengal where the lytic bacteriophage ICP1 specifically preys upon these pathogenic outbreak strains. ICP1 has also been the dominant bacteriophage found in cholera patient stool since 2001. However, little is known about its genomic differences between ICP1 strains collected over time. Here we elucidate the pan-genome and phylogeny of ICP1 strains by aligning, annotating and analyzing the genomes of 19 distinct isolates collected between 2001 and 2012. Our results reveal that ICP1 isolates are highly conserved and possess a large core-genome as well as a smaller, somewhat flexible accessory-genome. Despite its overall conservation, ICP1 strains have managed to acquire a number of unknown genes as well as a CRISPR-Cas system, which is known to be critical for its ongoing struggle for co-evolutionary dominance over its host. This study describes a foundation on which to construct future molecular and bioinformatic studies of this V. cholerae-associated bacteriophages.

scholarly journals Genomics of the Argentinian cholera epidemic elucidate the contrasting dynamics of epidemic and endemic Vibrio cholerae

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Matthew J. Dorman ◽  
Daryl Domman ◽  
Tomás Poklepovich ◽  
Charlotte Tolley ◽  
Gisella Zolezzi ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Genome Sequences ◽  
Epidemic Disease ◽  
Epidemic Clone ◽  
Endemic Disease ◽  
Direct Relevance ◽  
Cholera Epidemic ◽  
Local Endemic ◽  
Endemic Cholera ◽  
Over Time

Abstract In order to control and eradicate epidemic cholera, we need to understand how epidemics begin, how they spread, and how they decline and eventually end. This requires extensive sampling of epidemic disease over time, alongside the background of endemic disease that may exist concurrently with the epidemic. The unique circumstances surrounding the Argentinian cholera epidemic of 1992–1998 presented an opportunity to do this. Here, we use 490 Argentinian V. cholerae genome sequences to characterise the variation within, and between, epidemic and endemic V. cholerae. We show that, during the 1992–1998 cholera epidemic, the invariant epidemic clone co-existed alongside highly diverse members of the Vibrio cholerae species in Argentina, and we contrast the clonality of epidemic V. cholerae with the background diversity of local endemic bacteria. Our findings refine and add nuance to our genomic definitions of epidemic and endemic cholera, and are of direct relevance to controlling current and future cholera epidemics.

scholarly journals Genomic characteristics of recently recognized Vibrio cholerae El Tor lineages associated with cholera in Bangladesh, 1991-2017

2021 ◽  
Author(s):  
Md Mamun Monir ◽  
Talal Hossain ◽  
Masatomo Morita ◽  
Makoto Ohnishi ◽  
Fatema-tuj Johura ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Genome Wide Association Study ◽  
Sequence Data ◽  
Genomic Analysis ◽  
Comparative Genomic ◽  
Nucleotide Polymorphisms ◽  
Fitness Advantage ◽  
Genome Phylogeny ◽  
El Tor ◽  
Endemic Cholera

Comparative genomic analysis of Vibrio cholerae El Tor associated with endemic cholera in Asia revealed two distinct lineages, one dominant in Bangladesh and the other in India. An in depth whole genome study of V. cholerae El Tor clinical strains isolated during endemic cholera in Bangladesh (1991 – 2017) included reference genome sequence data obtained online. Core genome phylogeny established using single nucleotide polymorphisms (SNPs) showed V. choleraeEl Tor strains comprised two lineages, BD-1 and BD-2, which, according to Bayesian phylodynamic analysis, originated from paraphyletic group BD-0 around 1981. BD-1 and BD-2 lineages overlapped temporally but were negatively associated as causative agents of cholera 2004-2017. Genome wide association study (GWAS) revealed 140 SNPs and 31 indels, resulting in gene alleles unique to BD-1 and BD-2. Regression analysis of root to tip distance and year of isolation indicated early BD-0 strains at the base, whereas BD-1 and BD-2 subsequently emerged and progressed by accumulating SNPs. Pangenome analysis provided evidence of gene acquisition by both BD-1 and BD-2, of which six crucial proteins of known function were predominant in BD-2. BD-1 and BD-2 diverged and have distinctively different genomic traits, namely heterogeneity in VSP-2, VPI-1, mobile elements, toxin encoding elements, and total gene abundance. In addition, the observed phage-inducible chromosomal island-like element (PLE1), and SXT ICE elements (ICETET) in BD-2 presumably provided a fitness advantage for the lineage to outcompete BD-1 as the etiological agent of the endemic cholera in Bangladesh, with implications for global cholera epidemiology.

scholarly journals Complete Genome Sequence of Lytic Bacteriophage VPUSM 8 against O1 El Tor Inaba Vibrio cholerae

2017 ◽  
Vol 5 (21) ◽  
Author(s):  
Ali Al-Fendi ◽  
Rafidah Hanim Shueb ◽  
Phiaw Chong Foo ◽  
Manickam Ravichandran ◽  
Chan Yean Yean
Keyword(s):  
Vibrio Cholerae ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Biocontrol Agent ◽  
Phage Therapy ◽  
Lytic Bacteriophage ◽  
Content Type ◽  
El Tor ◽  
Potential Use

ABSTRACT The complete genome sequence of bacteriophage VPUSM 8 against O1 El Tor Inaba Vibrio cholerae is reported here. The isolated VPUSM 8 has potential use in future phage therapy or as a biocontrol agent for the prevention and treatment of cholera.

scholarly journals An experimental study of the population and evolutionary dynamics of Vibrio cholerae O1 and the bacteriophage JSF4

2010 ◽  
Vol 277 (1698) ◽  
pp. 3247-3254 ◽  
Author(s):  
Yan Wei ◽  
Paolo Ocampo ◽  
Bruce R. Levin
Keyword(s):  
Vibrio Cholerae ◽  
Evolutionary Dynamics ◽  
Natural Habitat ◽  
Natural Populations ◽  
Resistant Bacteria ◽  
Lytic Phage ◽  
Lytic Bacteriophage ◽  
El Tor ◽  
Short Time

Studies of Vibrio cholerae in the environment and infected patients suggest that the waning of cholera outbreaks is associated with rise in the density of lytic bacteriophage. In accordance with mathematical models, there are seemingly realistic conditions where phage predation could be responsible for declines in the incidence of cholera. Here, we present the results of experiments with the El Tor strain of V. cholerae (N16961) and a naturally occurring lytic phage (JSF4), exploring the validity of the main premise of this model: that phage predation limits the density of V. cholerae populations. At one level, the results of our experiments are inconsistent with this hypothesis. JSF4-resistant V. cholerae evolve within a short time following their confrontation with these viruses and their populations become limited by resources rather than phage predation. At a larger scale, however, the results of our experiments are not inconsistent with the hypothesis that bacteriophage modulate outbreaks of cholera. We postulate that the resistant bacteria that evolved play an insignificant role in the ecology or pathogenicity of V. cholerae . Relative to the phage-sensitive cells from whence they are derived, the evolved JSF4-resistant V. cholerae have fitness costs and other characters that are likely to impair their ability to compete with the sensitive cells in their natural habitat and may be avirulent in human hosts. The results of this in vitro study make predictions that can be tested in natural populations of V. cholerae and cholera-infected patients.

Genomic Variability of Pathogenicity Islands in Nontoxigenic Strains of Vibrio cholerae O1 Biotype El Tor

2020 ◽  
Vol 56 (9) ◽  
pp. 1055-1069
Author(s):  
N. I. Smirnova ◽  
A. A. Kritsky ◽  
J. V. Alkhova ◽  
E. Yu. Agafonova ◽  
E. Yu. Shchelkanova ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Pathogenicity Islands ◽  
Genomic Variability ◽  
Vibrio Cholerae O1 ◽  
El Tor

RESISTANT VIBRIO CHOLERÆ EL TOR IN TANZANIA

The Lancet ◽  
1979 ◽  
Vol 314 (8134) ◽  
pp. 147-148 ◽  
Author(s):  
K.J. Towner ◽  
N.J. Pearson ◽  
F. O'Grady
Keyword(s):  
Vibrio Cholerae ◽  
El Tor

Vibrio cholerae O1 El Tor variant and emergence of Haitian ctxB variant in the strains isolated from South India

2015 ◽  
Vol 205 (2) ◽  
pp. 195-200 ◽  
Author(s):  
Debdutta Bhattacharya ◽  
Shuchismita Dey ◽  
Gururaja Perumal Pazhani ◽  
Thandavarayan Ramamurthy ◽  
Mahantesh V. Parande ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
South India ◽  
Vibrio Cholerae O1 ◽  
El Tor

scholarly journals Isolation of Vibrio cholerae O1 strains similar to pre-seventh pandemic El Tor strains during an outbreak of gastrointestinal disease in an island resort in Fiji

2006 ◽  
Vol 55 (11) ◽  
pp. 1559-1562 ◽  
Author(s):  
G. Balakrish Nair ◽  
Ashrafus Safa ◽  
N. A. Bhuiyan ◽  
Suraia Nusrin ◽  
Denise Murphy ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Gastrointestinal Disease ◽  
Vibrio Cholerae O1 ◽  
El Tor

The evolution of the pathogen and the clinical and epidemiological features of the recent cholera (el tor)

2012 ◽  
Vol 17 (5) ◽  
pp. 31-35
Author(s):  
V. N. Savelyev ◽  
I. V. Savelyeva ◽  
B. V. Babenyshev ◽  
A. N. Kulichenko
Keyword(s):  
Genetic Structure ◽  
Vibrio Cholerae ◽  
Causative Agent ◽  
Genetically Modified ◽  
Causative Factor ◽  
Prevention Measures ◽  
Cholera Outbreak ◽  
The Caucasus ◽  
Epidemiological Features ◽  
El Tor

In a comparative perspective studied cholera outbreak in the Caucasus due to typical toxigenic and genetically modified (hybrid) El Tor variant strains have been studied. Revealed features of the genetic structure of the genome, factors and ways of transmission of the causative agent of modern cholera El tor should be considered when improving the program of epidemiological supervision in terms of enhancing antiepidemic and prevention measures in cholera, the causative factor of which are of hybrid variants of Vibrio cholerae El tor.

Sign in / Sign up

Export Citation Format

Share Document