scholarly journals Environmental plasticity and colonisation history in the Atlantic salmon microbiome: a translocation experiment

2019 ◽  
Author(s):  
Tamsyn M. Uren Webster ◽  
Deiene Rodriguez-Barreto ◽  
Giovanni Castaldo ◽  
John Taylor ◽  
Peter Gough ◽  
...  
Keyword(s):  
Atlantic Salmon ◽  
Early Life ◽  
Mammalian Species ◽  
Common Garden ◽  
Skin Microbiome ◽  
Alpha And Beta Diversity ◽  
Historical Processes ◽  
Before And After ◽  
First Time

AbstractMicrobial communities associated with the gut and the skin are strongly influenced by environmental factors, and can rapidly adapt to change. Historical processes may also affect the microbiome. In particular, variation in microbial colonisation in early life has the potential to induce lasting effects on microbial assemblages. However, little is known about the relative extent of microbiome plasticity or the importance of historical colonisation effects following environmental change, especially for non-mammalian species. To investigate this we performed a reciprocal translocation of Atlantic salmon between captive and semi-natural conditions. Wild and hatchery-reared fry were transferred to three common garden experimental environments for six weeks: standard hatchery conditions, hatchery conditions with an enriched diet, and simulated wild conditions. We characterised the faecal and skin microbiome of individual fish before and after the environmental translocation, using a BACI (before-after-control-impact) design. We found evidence of extensive plasticity in both gut and skin microbiota, with the greatest changes in alpha and beta diversity associated with the largest changes in environment and diet. Microbiome richness and diversity were entirely determined by environment, with no detectable historical effects of fish origin. Microbiome structure was also strongly influenced by current environmental conditions but, for the first time in fish, we also found evidence of colonisation history, including a number of OTUs characteristic of captive rearing. These results may have important implications for host adaptation to local selective pressures, and also highlight how conditions during early life can have a long-term influence on the microbiome and, potentially, host health.

scholarly journals Early life stress causes persistent impacts on the microbiome of Atlantic salmon

2020 ◽  
Author(s):  
Tamsyn M. Uren Webster ◽  
Sofia Consuegra ◽  
Carlos Garcia de Leaniz
Keyword(s):  
Atlantic Salmon ◽  
Cold Stress ◽  
Microbial Communities ◽  
Life Stress ◽  
Early Life ◽  
Early Life Stress ◽  
Skin Microbiome ◽  
Farmed Fish ◽  
Fish Pathogens ◽  
Egg Shell

AbstractIntensively farmed fish are commonly stressed, often leading to immune impairment and increased susceptibility to disease. Microbial communities associated with the gut and skin are vital to host immune function, but little is known about how stress affects the fish microbiome, especially during the sensitive early life stages. We compared the effects of two aquaculture-relevant stressors on the gut and skin microbiome of Atlantic salmon fry: an acute cold stress during late embryogenesis, and a chronic environmental stress during the larval stage. Acute cold stress had a lasting effect on the structure of both the gut and the skin microbiome, likely due to disruption of the egg shell microbial communities which seed the initial colonisation of the teleost microbiome upon hatching. In contrast, chronic post hatch stress altered the structure of the gut microbiome, but not that of the skin. Both types of stressors promoted similar Gammaproteobacteria ASVs, particularly within the genera Acinetobacter and Aeromonas which include several important fish pathogens and, in the gut, reduced the abundance of Lactobacillales. This suggests that there may be common signatures of stress in the salmon microbiome, which may represent useful stress biomarkers in aquaculture.

scholarly journals Detection of a polymorphic site of the porcine <i>C8G</i> gene and evaluation of association with haemolytic complement activity

2012 ◽  
Vol 55 (3) ◽  
pp. 255-262
Author(s):  
V. A. K. Do ◽  
S. Ponsuksili ◽  
E. Muráni ◽  
H. T. P. Loan ◽  
R. M. Brunner ◽  
...  
Keyword(s):  
Mammalian Species ◽  
Membrane Attack Complex ◽  
Population Based ◽  
Target Cells ◽  
Classical Pathway ◽  
Complement Activity ◽  
Weak Evidence ◽  
Before And After ◽  
Family Based

Abstract. C8 is a component of the membrane attack complex (MAC) of the complement system, which causes lysis of the target cells. C8 consists of three subunits C8A, C8B, and C8G. This study focus on the porcine C8G gene aiming to identify its cDNA sequence, to detect single nucleotide polymorphism (SNP), and to analyse its association with haemolytic complement activity in the classical (CH50) and the alternative (AH50) pathway. The C8G is 840 bp in length encoding 202 amino acids. The C8G is similar (≥65 %) among mammalian species (pig, human, cattle, dog, and mouse) at both the cDNA and the protein level. One SNP was detected at nucleotide 423C→T (SNP c.423C>T; codon 124GAC→GAT). The SNP does not segregate among the European commercial breeds German Landrace and Pietrain but in the Vietnamese autochthonous breed Muong Khuong (Vietnamese potbelly pig) and an experimental F2 crossbreed population based on Duroc and Berlin Miniature Pig (DUMI). Haemolytic complement activity of animals of the DUMI populations obtained at about 6, 14 and 16 weeks of age before and after vaccinations showed short-termed increments due to the immune stimulation and long-term increase due to aging. Family based association tests indicate effects of C8G on AH50 and CH50 at 16 weeks of age immediately before PRRS vaccination (AH50_PRRS0, P=0.087; CH50_PRRS0, P=0.027). However, the results did not indicate a consistent effect of the respective alleles on haemolytic complement activity in the alternative and the classical pathway. The study provides weak evidence for the candidacy of the porcine C8G for innate immune response and disease resistance in pigs.

scholarly journals Component Permeabilities and Water Contents in periplaneta Integument: Role Of The Epidermis Re-Examined

1985 ◽  
Vol 117 (1) ◽  
pp. 155-169 ◽  
Author(s):  
J. MACHIN ◽  
G. J. LAMPERT ◽  
M. J. O'DONNELL
Keyword(s):  
In Vitro Techniques ◽  
Linear Relationships ◽  
Before And After ◽  
Pressure Lowering ◽  
First Time ◽  
Water Contents

Improved in vivo and in vitro techniques for measuring cuticular water permeability are described. Air flowing over a cuticle disc mounted in a holder, permitted elimination of unstirred layers, or corrections for them, for the first time. Conditions inside the holder were incompatible with the long-term health of the epidermal cells. Significantly, mean permeabilities of these discs did not differ from values obtained in vivo on the same cuticular plate. Overall cuticular permeability was apportioned between endocuticle and combined epicuticle and exocuticle on the basis of measurements made before and after solvent extraction of lipids. Under identical activity gradients, endocuticle permeability was 35 to 40 times greater than the value for the other layer. Permeability of both component layers showed strongly non-linear relationships with ambient activity, with empirical proportionality to the reciprocal of vapour pressure lowering. Cuticle water contents measured in activity gradient conditions showed significantly higher values in vivo than in vitro. The amount of water contained in the combined epicuticle and exocuticle was too small to measure. We conclude that neither permeability nor water content data support the existence of a significant water barrier in the region of the epidermis.

scholarly journals Environment-driven reprogramming of gamete DNA methylation occurs during maturation and is transmitted intergenerationally in Atlantic Salmon

2021 ◽  
Author(s):  
Kyle Wellband ◽  
David Roth ◽  
Tommi Linnansaari ◽  
R Allen Curry ◽  
Louis Bernatchez
Keyword(s):  
Dna Methylation ◽  
Atlantic Salmon ◽  
Early Life ◽  
Empirical Support ◽  
Differential Methylation ◽  
Transgenerational Plasticity ◽  
Phenotypic Differences ◽  
Genome Bisulfite Sequencing ◽  
First Time

Abstract An epigenetic basis for transgenerational plasticity in animals is widely theorized, but convincing empirical support is limited by taxa-specific differences in the presence and role of epigenetic mechanisms. In teleost fishes, DNA methylation generally does not undergo extensive reprogramming and has been linked with environmentally-induced intergenerational effects, but solely in the context of early life environmental differences. Using whole genome bisulfite sequencing, we demonstrate that differential methylation of sperm occurs in response to captivity during the maturation of Atlantic Salmon (Salmo salar), a species of major economic and conservation significance. We show that adult captive exposure further induces differential methylation in an F1 generation that is associated with fitness-related phenotypic differences. Some genes targeted with differential methylation were consistent with genes differential methylated in other salmonid fishes experiencing early-life hatchery rearing, as well as genes under selection in domesticated species. Our results support a mechanism of transgenerational plasticity mediated by intergenerational inheritance of DNA methylation acquired late in life for salmon. To our knowledge, this is the first-time environmental variation experienced later in life has been directly demonstrated to influence gamete DNA methylation in fish.

scholarly journals The Role of Every-Day Cosmetics in Altering the Skin Microbiome: A Study Using Biodiversity

Cosmetics ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 2 ◽  
Author(s):  
Christopher Wallen-Russell
Keyword(s):  
Independent Study ◽  
Skin Microbiome ◽  
Rrna Sequencing ◽  
Human Participants ◽  
Medical University ◽  
First Time ◽  
Larger Sample ◽  
Universal Standard

As described in previous work, the use of synthetic chemical ingredients in modern cosmetics is postulated to be a cause of damage to the skin microbiome. The discovery that biodiversity on the human skin is currently the only reliable indicator of skin health, meant that for the first time, a mechanism to test for healthy skin was possible. Using this mechanism and in collaboration with The Medical University of Graz, who carried out the independent study, this work aimed to help answer whether modern day synthetic cosmetics are a main cause of long-term damage to the skin microbiome. Thirty-two human participants tested three different face washes for their effect on the skin’s microbial diversity, along with skin pH, moisture and TEWL (trans-epidermal water loss), washing twice-a-day for four weeks. The upper volar forearm of the volunteers was swabbed at the beginning, two weeks in and at the end of the four weeks. 16S rRNA sequencing was used. One leading ‘natural’ brand full of synthetic ingredients, a leading synthetic brand and a 100% natural face wash were used. Results give the first indications of a link between synthetic ingredients in a cosmetics product and its effect on skin microbiome biodiversity. It paves the way for future studies on the topic with a larger sample group, longer test period and standardised methodology to create a universal standard for testing the health of skin using benchmark diversity values. This can be used in the future to test the effectiveness of cosmetics or ingredients on skin health, leading to the restriction in cosmetics of products proven to harm the skin’s natural environment.

scholarly journals Stable reprogramming of brain transcription profiles by the early social environment in a cooperatively breeding fish

2013 ◽  
Vol 280 (1753) ◽  
pp. 20122605 ◽  
Author(s):  
Barbara Taborsky ◽  
Linda Tschirren ◽  
Clémence Meunier ◽  
Nadia Aubin-Horth
Keyword(s):  
Early Life ◽  
Social Experience ◽  
Stress Axis ◽  
Expression Ratio ◽  
Transcription Profiles ◽  
Brain Expression ◽  
Cooperatively Breeding ◽  
Hpi Axis ◽  
First Time

Adult social behaviour can be persistently modified by early-life social experience. In rodents, such effects are induced by tactile maternal stimulation resulting in neuroendocrine modifications of the hypothalamic–pituitary–adrenal axis involved in stress responsiveness. Whether similar long-term alterations can occur in the hypothalamic–pituitary–interrenal (HPI) axis of poikilothermic vertebrates is unknown. We compared the expression of four genes of the HPI axis in adults of the cooperatively breeding cichlid Neolamprologus pulcher , which had been exposed to two early-life social treatments 1.5 years prior to brain sampling. Fish reared with parents and siblings had less brain expression of corticotropin-releasing factor and of the functional homologue of the mammalian glucocorticoid receptor (GR1) than individuals reared with same-age siblings only. Expression of the mineralocorticoid receptors (MR) did not differ between treatments, but the MR/GR1 expression ratio was markedly higher in fish reared with parents and siblings. Thus, we show here that early social experience can alter the programming of the stress axis in poikilothermic vertebrates, suggesting that this mechanism is deeply conserved within vertebrates. Moreover, we show for the first time that reprogramming of the stress axis of a vertebrate can be induced without tactile stimulation by parents.

scholarly journals Spatio-temporal variation of rainfall over Bihar State, India

2018 ◽  
Vol 36 (1) ◽  
pp. 183-197 ◽  
Author(s):  
Pratibha Warwade ◽  
Shalini Tiwari ◽  
Sunil Ranjan ◽  
Surendra K. Chandniha ◽  
Jan Adamowski
Keyword(s):  
Change Point ◽  
Winter Rainfall ◽  
Rainfall Trends ◽  
Before And After ◽  
Spatio Temporal ◽  
Bihar State ◽  
First Time ◽  
Cumulative Deviation ◽  
Shift Change

AbstractThis study detected, for the first time, the long term annual and seasonal rainfall trends over Bihar state, India, between 1901 and 2002. The shift change point was identified with the cumulative deviation test (cumulative sum – CUSUM), and linear regression. After the shift change point was detected, the time series was subdivided into two groups: before and after the change point. Arc-Map 10.3 was used to evaluate the spatial distribution of the trends. It was found that annual and monsoon rainfall trends decreased significantly; no significant trends were observed in pre-monsoon, monsoon, post-monsoon and winter rainfall. The average decline in rainfall rate was –2.17 mm·year−1and –2.13 mm·year−1for the annual and monsoon periods. The probable change point was 1956. The number of negative extreme events were higher in the later period (1957–2002) than the earlier period (1901–1956).

scholarly journals Bone formation and resorption biological markers in cosmonauts during and after a 180-day space flight (Euromir 95)

1998 ◽  
Vol 44 (3) ◽  
pp. 578-585 ◽  
Author(s):  
Anne Caillot-Augusseau ◽  
Marie-Héléne Lafage-Proust ◽  
Claude Soler ◽  
Josiane Pernod ◽  
Francis Dubois ◽  
...  
Keyword(s):  
Bone Loss ◽  
Bone Formation ◽  
Bone Remodeling ◽  
Bone Alkaline Phosphatase ◽  
Serum Concentrations ◽  
Intact Osteocalcin ◽  
Before And After ◽  
First Time

Abstract Long-term spaceflights induce bone loss as a result of profound modifications of bone remodeling, the modalities of which remain unknown in humans. We measured intact parathyroid hormone (PTH) and serum calcium; for bone formation, serum concentrations of bone alkaline phosphatase (BAP), intact osteocalcin (iBGP), and type 1 procollagen propeptide (PICP); for resorption, urinary concentrations (normalized by creatinine) of procollagen C-telopeptide (CTX), free and bound deoxypyridinoline (F and B D-Pyr), and Pyr in a 36-year-old cosmonaut (RTO), before (days −180, −60, and −15), during (from days 10 to 178, n = 12), and after (days +7, +15, +25, and +90) a 180-day spaceflight, in another cosmonaut (ASW) before and after the flight. Flight PTH tended to decrease by 48% and postflight PTH increased by 98%. During the flight, BAP, iBGP, and PICP decreased by 27%, 38%, and 28% respectively in CM1, and increased by 54%, 35%, and 78% after the flight. F D-Pyr and CTX increased by 54% and 78% during the flight and decreased by 29% and 40% after the flight, respectively. We showed for the first time in humans that microgravity induced an uncoupling of bone remodeling between formation and resorption that could account for bone loss.

scholarly journals The Role of Every-Day Cosmetics in Altering the Skin Microbiome: A Study Using Biodiversity

2018 ◽  
Author(s):  
Christopher Wallen-Russell
Keyword(s):  
Independent Study ◽  
Skin Microbiome ◽  
Rrna Sequencing ◽  
Human Participants ◽  
Medical University ◽  
First Time ◽  
Larger Sample ◽  
Universal Standard

As described in previous work, the use of synthetic chemical ingredients in modern cosmetics is postulated to be a cause of damage to the skin microbiome. The discovery that biodiversity on the human skin is currently the only reliable indicator of skin health, meant that for the first time, a mechanism to test for healthy skin was possible. Using this mechanism and in collaboration with The Medical University of Graz, who carried out the independent study, this work aimed to help answer whether modern day synthetic cosmetics are a main cause of long term damage to the skin microbiome. Thirty-two human participants tested three different face washes for their effect on the skin&rsquo;s microbial diversity, along with skin pH, moisture and TEWL (trans-epidermal water loss), washing twice a day for four weeks. The upper volar forearm of the volunteers was swabbed at the beginning, two weeks in and end (four weeks). 16S rRNA sequencing was used. One leading &lsquo;natural&rsquo; brand full of synthetic ingredients, a leading synthetic brand and a 100% natural face wash were used. Results give the first indications of a link between synthetic ingredients in a cosmetics product, and its effect on skin microbiome biodiversity. It paves the way for future studies on the topic with a larger sample group, longer test period and standardised methodology to create a universal standard for testing the health of skin using benchmark diversity values. This can be used in the future to test the effectiveness of cosmetics or ingredients on skin health, leading to the banning of products proven to harm the skin&rsquo;s natural environment.

Untersuchungen zur Quantifizierung der Änderung der Skelettmuskeldurchblutung nach Gefäßrevaskularisationen mit einem 99mTechnetiummarkierten Tracer

1990 ◽  
Vol 29 (05) ◽  
pp. 215-220 ◽  
Author(s):  
R. Benning ◽  
K. Nagel ◽  
M. Jugenheimer ◽  
S. Fischer ◽  
S. Worthmann ◽  
...  
Keyword(s):  
Transluminal Angioplasty ◽  
Lower Limbs ◽  
Surgical Patients ◽  
Treadmill Test ◽  
Negative Results ◽  
Clinical Observations ◽  
Before And After ◽  
Comparative Results ◽  
First Time ◽  
Percutaneous Transluminal

A new 99mTc-labelled tracer (99mTc-Sestanriibi) was used for the first time to demonstrate the perfusion of the skeletal muscle. In 16 patients with obstructive atherosclerosis of the lower limbs the change of perfusion of thigh and lower leg was studied with SPECT before and after vascular surgery (n = 11) or percutaneous transluminal angioplasty (n = 5). Comparative results of scintigraphic measurements and clinical observations (ancle-arm pressure, treadmill test) in 10 surgical patients (14 operated legs) showed correct positive or negative results in 86% (12/14).

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