scholarly journals Sperm quality parameters are increased and asymmetric in house mouse hybrids

2019 ◽  
Author(s):  
Iva Martincová ◽  
Ľudovít Ďureje ◽  
Stuart J. E. Baird ◽  
Jaroslav Piálek
Keyword(s):  
Y Chromosome ◽  
House Mouse ◽  
Sperm Quality ◽  
Quality Parameters ◽  
F1 Hybrids ◽  
Secondary Contact ◽  
Male Fitness ◽  
Cross Direction ◽  
Sperm Trait ◽  
The Difference

AbstractSpermatogenesis is a tuned cascade of processes producing sperm; impairment of any phase of this process can affect fitness of males. The level of impairment can be pronounced in hybrids between genetically divergent populations. To explore the effect of hybridization on sperm quality we produced F1 hybrids from 29 wild derived strains of two house mouse subspecies, M. m. musculus and M. m. domesticus, which diverged 0.5 MY ago. The measured sperm quality traits did not significantly differ between intrasubspecific crosses. Effects of intersubspecific hybridization were dependent on sperm trait and cross direction. The proportion of sperm head abnormalities was increased in F1 intersubspecific hybrids. The frequency of dissociated sperm heads was increased in the M. m. musculus × M. m. domesticus (♀×♂) F1 but decreased in M. m. domesticus × M. m. musculus (♀×♂) F1 hybrids, with the difference in medians being more than 180%. We deduce that the dissociated sperm heads trait is associated with the X chromosome and modulated by interaction with the Y chromosome; nevertheless, the high proportion of unexplained variance (55.46 %) suggests the presence of polymorphic autosomal interactions. The reported differences in sperm quality between cross types may be highly relevant to male fitness in zones of secondary contact between the two subspecies. The cross direction asymmetry in frequency of dissociated sperm heads should favour the M. m. musculus Y chromosome. This is consistent with the spread of the M. m. musculus Y chromosome in nature across the hybrid zone between these two subspecies.

Is p53 controlling spermatogenesis in male mice with the deletion on the Y chromosome?

Zygote ◽  
2011 ◽  
Vol 21 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Tomasz Lech ◽  
Aniela Golas ◽  
Jozefa Styrna
Keyword(s):  
Y Chromosome ◽  
Semen Quality ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Quality Parameters ◽  
Quality Parameter ◽  
Sperm Head ◽  
Testis Weight ◽  
Male Mice ◽  
Chromosome Y

SummaryThe aim of the study was to evaluate the influence of the chromosome Y structure and Trp53 genotype on semen quality parameters. Mice with partial deletion of the Y chromosome (B10.BR-Ydel) have severely altered sperm head morphology when compared with males that possess the complete Y chromosome (B10.BR). Control males from B10.BR and B10.BR-Ydel mice, and mutant males from B10.BR-p53−/− and B10.BR-Ydel-p53−/− experimental groups were used. We assessed testis weight, sperm head abnormalities, viability of spermatozoa (eosin test), percentage of motile and immature sperm, and performed a hypo-osmotic test to detect abnormal tail membrane integrity. Sperm morphology and maturation were controlled by the genes within the deleted region of the Y chromosome. Testis weight was higher in the mutants than in the control males, possibly due to cell accumulation in Trp53-deficient males as the concentration of sperm was significantly increased in the mutants. An elevated percentage of abnormal sperm was noted in B10.BR-p53−/− and B10.BR-Ydel-p53−/− male mice. We suggest that, in Trp53-deficient mice, the sperm cells that escape apoptosis are the ones that have abnormal morphology. The only sperm quality parameter affected by the interplay between Trp53 and chromosome Y genes was sperm motility, which was elevated in B10.BR-p53−/− males, but remained unchanged in B10.BR-Ydel-p53−/− males.

scholarly journals Sperm quality, aggressiveness and generation turnover may facilitate unidirectional Y chromosome introgression across the European house mouse hybrid zone

Heredity ◽  
2020 ◽  
Vol 125 (4) ◽  
pp. 200-211 ◽  
Author(s):  
Barbora Vošlajerová Bímová ◽  
Miloš Macholán ◽  
Ľudovít Ďureje ◽  
Kateřina Berchová Bímová ◽  
Iva Martincová ◽  
...  
Keyword(s):  
Y Chromosome ◽  
House Mouse ◽  
Hybrid Zone ◽  
Sperm Quality ◽  
Chromosome Introgression

scholarly journals Budidaya ikan mas (Cyprinus carpio) sistem akuaponik dengan padat penebaran berbeda

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Darwis Darwis ◽  
Joppy D. Mudeng ◽  
Sammy N.J. Londong
Keyword(s):  
Water Quality ◽  
Cyprinus Carpio ◽  
Stocking Density ◽  
Quality Parameters ◽  
Water Volume ◽  
Daily Growth ◽  
Growth And Survival ◽  
Experimental Fish ◽  
The Difference

This research aimed to determine the stocking density that support the best growth and survival rate of carp cultivated in aquaponic systems, and to study the water quality of carp culture with different stocking densities in aquaponic systems. The study used 12 aquaria measuring 40x40x40 cm each (water volume of 50 liters). The experimental fish are carp (Cyprinus carpio) weighing in average of 3.5 g/individual.  The fish were cultivated with different stocking densities as treatment, including A = 4; B = 7; C = 10 and D = 13 individuals/aquarium.  The fish were fed with pellet at 5%/body weight/day with a feeding frequency of two times a day. The study was conducted for 4 weeks. Data consisting of daily growth, survival and water quality parameters (temperature, pH, dissolved oxygen, ammonia, nitrite and nitrate) were measured once a week. The data obtained were analyzed by ANOVA. The results of the study showed that the difference in stocking density had no significant effect on the growth and survival of carp.  Water quality was in a reasonable range to support the growth and survival of carp cultivated with aquaponic systems.Keywords: cultivation, carp, aquaponics, stocking density, growth

scholarly journals Evaluating bull fertility based on non-return method

2012 ◽  
Vol 66 (1-2) ◽  
pp. 27-40
Author(s):  
Igor Prka ◽  
Dragan Vukovic ◽  
Stevan Perkovic
Keyword(s):  
Semen Quality ◽  
Sperm Quality ◽  
Quality Parameters ◽  
Semen Parameters ◽  
Service Period ◽  
Microscopic Evaluation ◽  
Live Sperm ◽  
Return Method ◽  
The Impact ◽  
First Time

In order to evaluate the results of reproductive cows and heifers, different parameters of fertility are used, such as the service period, insemination index, intercalving time and others, and of the breeding bulls the values obtained through non-return. An ejaculate is taken up for further processing by veterinary centres only provided it meets the prescribed quality parameters. Rating semen parameters includes a macroscopic (volume, colour, consistency, smell and pH) and a microscopic evaluation (mobility, density, percentage of live sperm and abnormal and damaged sperm). In addition to sperm quality and the fertility of the female animal, the results of the non-return method are also influenced by a number of exogenous causes (season, age, race, insemination techniques) that have no small impact on the end result of insemination - pregnancy. In order to obtain more objective results of the fertility of bulls the following tasks were undertaken, namely: 1. to calculate with the non-return method the fertility of bulls in over 10,000 cows inseminated for the first time during a period of 6 years; and 2. to analyze the impact of semen quality, season, age of cow and bull, and the bull breed on the results of fertility.

Fluoxetine treatment of prepubertal male rats uniformly diminishes sex hormone levels and, in a subpopulation of animals, negatively affects sperm quality

2018 ◽  
Vol 30 (10) ◽  
pp. 1329 ◽  
Author(s):  
María E. Ayala ◽  
Ayari Gonzáles ◽  
Rodrigo M. Olivarez ◽  
Andrés Aragón-Martínez
Keyword(s):  
Adult Stage ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Graphical Analysis ◽  
Quality Parameters ◽  
Male Rats ◽  
Serum Concentrations ◽  
Sperm Density ◽  
Sexual Steroids ◽  
Fluoxetine Treatment

Fluoxetine (Flx) is a selective serotonin reuptake inhibitor that alters the male reproductive system when administered at the adult stage or after maternal exposure. In the present study we evaluated the effects of Flx administration on reproductive parameters during juvenile–peripubertal development when treated male rats reached adulthood. Groups of rats were treated daily with Flx (5 mg kg−1, i.p.) or saline (0.9% NaCl), or were left untreated. Rats were treated between 30 and 53 days of age and were killed at 65 days of age. Serotonin concentrations were determined in the hypothalamus, hypophysis and testis. Gonadotrophins, sex steroids and sperm quality (membrane integrity, sperm with functional mitochondria, sperm density, sperm motility and morphological abnormalities) were also evaluated. Flx did not affect bodyweight, but significantly diminished LH, FSH, progesterone and testosterone serum concentrations. After graphical analysis, a subgroup of rats was identified whose sperm quality parameters were greatly affected by Flx. In the present study we show that Flx administered to juvenile rats disrupts the hypothalamic–hypophyseal–testicular axis and its effects on sperm quality are not homogeneous in adults. In contrast, Flx altered concentrations of gonadotrophins and sexual steroids in all treated rats. These results suggest caution should be exercised in the prescription of Flx to prepubertal males.

scholarly journals Whole-genome sequencing analysis of Y chromosome microdeletion: a case report

2020 ◽  
Vol 48 (12) ◽  
pp. 030006052097649
Author(s):  
Zhixiang Gao ◽  
Feng Yuan ◽  
Qiaoqiao Li ◽  
Renlan Xia ◽  
Kai Fu ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Y Chromosome ◽  
Genome Sequencing ◽  
Sperm Quality ◽  
Sperm Count ◽  
Therapeutic Targets ◽  
Camp Signaling ◽  
Whole Genome ◽  
Sequencing Analysis ◽  
Y Chromosome Microdeletion

The mechanisms by which Y chromosome microdeletions cause infertility have been well described; however, the therapeutic targets remain a challenge. Here, we used whole-genome sequencing to explore the mechanism of Y chromosome deletion and potential therapeutic targets in a patient with infertility. There were no abnormalities in the patient’s medical history. Routine semen analysis showed immotile sperm and only two motile spermatozoa were occasionally see after centrifugation, indicating that the direct cause of infertility was an abnormal sperm count and motility. A Y chromosome microdeletion test revealed partial deletion of the AZFc region, including AZFc1, AZFc2, AZFc3, and AZFc4. Whole-genome sequencing showed that the patient had seven harmful mutations, with only one significant epigenetic mutation, SH3KBP1. Gene Ontology analysis of these meaningful mutations indicated involvement of cAMP signaling pathways. The patient’s wife became pregnant following in vitro fertilization, and no significant abnormalities were found during prenatal examination. This case suggests that Y chromosome microdeletion and gene mutation may affect the cAMP signaling pathway, leading to reduced sperm quality and male infertility.

scholarly journals A new approach for the removal of unfixed dyes from reactive dyed cotton by Fenton oxidation

2019 ◽  
Vol 9 (2) ◽  
pp. 133-141
Author(s):  
Sana Islam ◽  
Irfan Ahmed Shaikh ◽  
Nabeela Firdous ◽  
Azhar Ali ◽  
Yumna Sadef
Keyword(s):  
Treatment Time ◽  
Color Difference ◽  
Quality Parameters ◽  
Water Levels ◽  
Fenton Oxidation ◽  
Color Strength ◽  
New Approach ◽  
Reactive Dyeing ◽  
The Difference ◽  
Day By Day

Abstract The use of fresh water in the textile wash-off process is becoming more expensive day by day due to declining water levels in the region. In this study, the potential of using Fenton oxidation in wash-off cotton reactive dyeing was investigated. The spent wash-off wastewater from one dyeing was first treated with Fenton oxidation, and then reused in several washing-offs employing widely used reactive dyes, C.I. Reactive Yellow 145, C.I. Reactive Blue 21, and C.I. Reactive Red 195. Experimental results showed that at acidic pH (3) using optimized quantities of FeSO4 and H2O2, Fenton process yielded a significant reduction (90–95%) of color in 30 minutes of treatment time. New washing-offs were then carried out in Fenton decolorized wash-off wastewater, and dyed cotton fabric samples were subjected to quality evaluations in terms of color difference properties (ΔL*, Δc*,Δb*, Δa*, ΔE*cmc) and wash fastness properties. This study concluded that after Fenton oxidation, treated liquor can be effectively reused subsequent washing-offs without compromising fabric quality parameters as ΔE*cmc was less than 1, and washing and crocking was also in the range of 4.5–5 which is commercially acceptable. Moreover, the difference in color strength in terms of k/s was also negligible.

scholarly journals New Approach in COD Fractionation Methods

Water ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1484 ◽  
Author(s):  
Ewelina Płuciennik-Koropczuk ◽  
Sylwia Myszograj
Keyword(s):  
Degradation Rate ◽  
Oxygen Demand ◽  
Quality Parameters ◽  
Treated Wastewater ◽  
Detailed Knowledge ◽  
New Approach ◽  
High Compliance ◽  
The Difference ◽  
Biochemical Degradation ◽  
Raw Wastewater

Conventional quality parameters such as Chemical Oxygen Demand (COD) or Biochemical Oxygen Demand (BOD) give information about the quantity of organic matter present in wastewater, but do not give a clear indication of the biodegradability of the pollutants flowing in the WWTP. Detailed knowledge can be obtained by dividing the total COD into fractions. Fractionation and balancing of COD can be determined in various ways and with varying accuracy. Good wastewater characteristics are obtained on the basis of COD fractionation in accordance with ATV-A 131 guidelines, especially when the wastewater characteristics are in high compliance with the assumptions of the method. The article proposes a modification of the ATV-A131 method that increases the accuracy of determining the COD fraction. In order to reduce errors in the calculation of COD fractions, the value of fraction XS was calculated on the basis of the biochemical degradation rate determined in studies (k) for raw wastewater, whereas the SI fraction was calculated from the difference between SCOD and BODTot of filtered treated wastewater. BODTot of the treated wastewater was calculated taking into account the rate of biochemical degradation determined in the studies (k) for treated wastewater. The shares of individual COD fractions in raw wastewater calculated on the basis of the standard and modified procedure differed by approx. 10% in the case of suspension fractions. Modification of the methodology to determine the COD of the treated wastewater SS fraction significantly influenced the contents of all fractions in treated wastewater.

scholarly journals Retrieval and Validation of Water Turbidity at Metre-Scale Using Pléiades Satellite Data: A Case Study in the Gironde Estuary

2020 ◽  
Vol 12 (6) ◽  
pp. 946 ◽  
Author(s):  
Yafei Luo ◽  
David Doxaran ◽  
Quinten Vanhellemont
Keyword(s):  
Spatial Variability ◽  
Satellite Data ◽  
Near Infrared ◽  
Atmospheric Correction ◽  
Field Measurements ◽  
Quality Parameters ◽  
Satellite Constellations ◽  
Water Turbidity ◽  
Gironde Estuary ◽  
The Difference

This study investigated the use of frequent metre-scale resolution Pléiades satellite imagery to monitor water quality parameters in the highly turbid Gironde Estuary (GE, SW France). Pléiades satellite data were processed and analyzed in two representative test sites of the GE: 1) the maximum turbidity zone and 2) the mouth of the estuary. The main objectives of this study were to: (i) validate the Dark Spectrum Fitting (DSF) atmospheric correction developed by Vanhellemont and Ruddick (2018) applied to Pléiades satellite data recorded over the GE; (ii) highlight the benefits of frequent metre-scale Pléiades observations in highly turbid estuaries by comparing them to previously validated satellite observations made at medium (250/300 m for MODIS, MERIS, OLCI data) and high (20/30 m for SPOT, OLI and MSI data) spatial resolutions. The results show that the DSF allows for an accurate retrieval of water turbidity by inversion of the water reflectance in the near-infrared (NIR) and red wavebands. The difference between Pléiades-derived turbidity and field measurements was proven to be in the order of 10%. To evaluate the spatial variability of water turbidity at metre scale, Pléiades data at 2 m resolution were resampled to 20 m and 250 m to simulate typical coarser resolution sensors. On average, the derived spatial variability in the GE is lower than or equal to 10% and 26%, respectively, in 20-m and 250-m aggregated pixels. Pléiades products not only show, in great detail, the turbidity features in the estuary and river plume, they also allow to map the turbidity inside ports and capture the complex spatial variations of turbidity along the shores of the estuary. Furthermore, the daily acquisition capabilities may provide additional advantages over other satellite constellations when monitoring highly dynamic estuarine systems.

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