scholarly journals Isolation and quantification of blood apoptotic bodies in neurological patients

2019 ◽  
Author(s):  
Gemma Serrano-Heras ◽  
Inmaculada Díaz-Maroto ◽  
Blanca Carrión ◽  
Ana B. Perona-Moratalla ◽  
Julia Gracia ◽  
...  
Keyword(s):  
Scientific Literature ◽  
Apoptotic Bodies ◽  
Blood Samples ◽  
Rapid Procedure ◽  
Drug Effectiveness ◽  
Non Invasive ◽  
Apoptotic Death ◽  
Neurological Patients ◽  
Human Blood Samples

AbstractDysregulation of apoptosis may contribute to the etiology and/or progression of several prevalent diseases, including stroke and neurodegenerative pathologies. So, detection of the apoptotic processes in patients would be useful in daily clinical practice, However, the in vivo analysis of apoptosis that occurs in tissue has limitations. We therefore propose to use circulating apoptotic bodies as biomarkers for measuring apoptotic death in patients. Since there is no scientific literature establishing the most appropriate method for measuring apoptotic bodies from human blood samples, we here describe a reproducible centrifugation-based method combined with electron microscopy, dynamic light scattering and flow cytometry studies to isolate, characterize and quantify plasma apoptotic bodies of patients with ischemic stroke, multiple sclerosis and Parkinson’s disease. The analysis revealed that our isolation protocol achieves notable recovery rates of highly-purified intact apoptotic bodies. This easy and rapid procedure would help physicians to implement the use of plasma apoptotic bodies analysis as a non-invasive tool to monitor apoptotic death in neurological patients for prognostic purposes and for following disease activity and assessing drug effectiveness.

scholarly journals Protein Adductomics: Methodologies for Untargeted Screening of Adducts to Serum Albumin and Hemoglobin in Human Blood Samples

2019 ◽  
Vol 8 (1) ◽  
pp. 6 ◽  
Author(s):  
Henrik Carlsson ◽  
Stephen Rappaport ◽  
Margareta Törnqvist
Keyword(s):  
Mass Spectrometry ◽  
Serum Albumin ◽  
Human Blood ◽  
Mass Spectrometry Data ◽  
Reaction Products ◽  
Blood Samples ◽  
Large Numbers ◽  
Human Blood Samples ◽  
Critical Aspects

The reaction products of electrophiles in vivo can be measured as adducts to the abundant proteins, hemoglobin (Hb), and human serum albumin (HSA), in human blood samples. During the last decade, methods for untargeted screening of such adducts, called “adductomics”, have used liquid chromatography-mass spectrometry to detect large numbers of previously unknown Hb and HSA adducts. This review presents methodologies that were developed and used in our laboratories for Hb and HSA adductomics, respectively. We discuss critical aspects regarding choice of target protein, sample preparation, mass spectrometry, data evaluation, and strategies for identification of detected unknown adducts. With this review we give an overview of these two methodologies used for protein adductomics and the precursor electrophiles that have been elucidated from the adducts.

A highly sensitive red-emitting probe for the detection of viscosity changes in living cells, zebrafish, and human blood samples

2020 ◽  
Vol 8 (6) ◽  
pp. 1310-1315 ◽  
Author(s):  
Manlin Fu ◽  
Wei Shen ◽  
Yajun Chen ◽  
Wenjun Yi ◽  
Chunhui Cai ◽  
...  
Keyword(s):  
Human Blood ◽  
Fluorescent Probes ◽  
Living Cells ◽  
The State ◽  
Blood Samples ◽  
Highly Sensitive ◽  
Human Blood Samples ◽  
Viscosity Changes

Intracellular viscosity can be measured to reflect the state of living cells. Fluorescent probes are powerful tools for viscosity detection in vivo.

scholarly journals Application of microRNA in Human Osteoporosis and Fragility Fracture: A Systemic Review of Literatures

2021 ◽  
Vol 22 (10) ◽  
pp. 5232
Author(s):  
Yen-Zung Wu ◽  
Hsuan-Ti Huang ◽  
Tsung-Lin Cheng ◽  
Yen-Mou Lu ◽  
Sung-Yen Lin ◽  
...  
Keyword(s):  
Human Blood ◽  
Diagnostic Value ◽  
Systemic Review ◽  
Blood Samples ◽  
In Vivo Experiments ◽  
Pubmed Database ◽  
Entry Points ◽  
Human Blood Samples

MicroRNAs (miRNAs) could serve as ideal entry points to the deregulated pathways in osteoporosis due to their relatively simple upstream and downstream relationships with other molecules in the signaling cascades. Our study aimed to give a comprehensive review of the already identified miRNAs in osteoporosis from human blood samples and provide useful information for their clinical application. A systematic literature search for relevant studies was conducted in the Pubmed database from inception to December 2020. We set two essential inclusion criteria: human blood sampling and design of controlled studies. We sorted the results of analysis on human blood samples according to the study settings and compiled the most promising miRNAs with analyzed diagnostic values. Furthermore, in vitro and in vivo evidence for the mechanisms of the identified miRNAs was also illustrated. Based on both diagnostic value and evidence of mechanism from in vitro and in vivo experiments, miR-23b-3p, miR-140-3p, miR-300, miR-155-5p, miR-208a-3p, and miR-637 were preferred candidates in diagnostic panels and as therapeutic agents. Further studies are needed to build sound foundations for the clinical usage of miRNAs in osteoporosis.

scholarly journals EPR and FTIR Spectroscopies Study of Human Blood after Irradiation

2012 ◽  
Vol 27 ◽  
pp. 367-371 ◽  
Author(s):  
M. Polakovs ◽  
N. Mironova-Ulmane ◽  
A. Pavlenko ◽  
E. Reinholds ◽  
M. Gavare ◽  
...  
Keyword(s):  
Human Blood ◽  
Hierarchical Cluster ◽  
Time Saving ◽  
Paramagnetic Resonance ◽  
Blood Samples ◽  
Before And After ◽  
Human Blood Samples ◽  
Concentration Changes ◽  
Electron Paramagnetic

In the present work we report results of investigations of human blood before and after radioisotopeTc99mdiagnosis by electron paramagnetic resonance (EPR) and Fourier transform infrared (FTIR) spectroscopies. It is shown that EPR can detect the concentration of methaemoglobin and transferrin ions more accurately than any other technique. FTIR spectra indicated that radiation caused conformational and concentration changes of proteins. Hierarchical cluster analysis (HCA) was created as time-saving tool for discrimination of initial and irradiatedin vivohuman blood samples.

The Effect of Alimentary Hyperlipemia on Thrombolysis in vivo

1960 ◽  
Vol 04 (02) ◽  
pp. 149-166 ◽  
Author(s):  
Nils U. Bang ◽  
Eugene E. Cliffton
Keyword(s):  
Fibrinolytic Activity ◽  
Fibrinolytic Enzyme ◽  
Enzyme Treatment ◽  
Enzyme Therapy ◽  
Fasting State ◽  
Fatty Meal ◽  
Complete Dissolution ◽  
Blood Samples ◽  
Specific Inhibitors

Summary1. The effect of a standard, potent fibrinolytic enzyme therapy has been compared in fasting and lipemic dogs.2. The standard fibrinolytic regimen resulted in the complete dissolution of all clots produced experimentally in the fasting state in 10 dogs.3. Clots formed during alimentary lipemia exhibited a markedly increased resistance to the standard fibrinolytic regimen in 6 dogs.4. An increase in anti plasmin fibrinolytic titer with concomitant decrease in spontaneous fibrinolytic activity was observed in 15 dogs following the administration of a fatty meal. No difference in fibrinolytic activity and APF titer was demonstrable in fasting and lipemic blood samples obtained during fibrinolytic enzyme treatment.5. The possibility of the presence of specific inhibitors against the fibrinolytic enzyme in clots formed during lipemia has been investigated and the evidence to support this theory is discussed.

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