scholarly journals Native state of natural proteins optimizes local entropy

2021 ◽  
Vol 104 (6) ◽  
Author(s):  
M. Negri ◽  
G. Tiana ◽  
R. Zecchina
Keyword(s):  
Local Entropy ◽  
Native State

Existence of DNA in yeast microbodies

1978 ◽  
Vol 36 (2) ◽  
pp. 232-233
Author(s):  
Masako Osumi ◽  
Misuzu Nagano ◽  
Hiroko Kazama
Keyword(s):  
Catalase Activity ◽  
Buoyant Density ◽  
Model System ◽  
Contour Length ◽  
Native State ◽  
Normal Alkane ◽  
Remarkable Increase ◽  
Dna Molecule ◽  
Mitochondrial Dnas

We have found that microbodies appeared profusely together with a remarkable increase in catalase activity in normal alkane-grown cells of hydrocarbon-utilizing Candida yeasts, and that the microbodies multiplied by division in these cells. These features of Candida yeasts seem to provide a useful model system for studies on the biogenesis of the microbody. Subsequently, we have succeeded in isolation of Candida microbodies in an apparently native state, as judged biochemically and morphologically. The presence of DNA in the purified microbody fraction thus obtained was proved by the diphenylamine method. DNA molecule of about 15 urn in contour length was released from an isolated microbody. The physicochemical analyses of the microbody DNA revealed that its buoyant density differed from nuclear and mitochondrial DNAs. All these results lead us to the possibility that there is a novel type of DNA in microbodies.

Electron Microscopic Observation of Biological Specimens in Their Native State by Employing Cryogenic Techniques

1972 ◽  
Vol 30 ◽  
pp. 410-411 ◽  
Author(s):  
Tokio Nei ◽  
Haruo Yotsumoto ◽  
Yoichi Hasegawa ◽  
Yuji Nagasawa
Keyword(s):  
Electron Microscopic Observation ◽  
Surface Structures ◽  
Specimen Preparation ◽  
Native State ◽  
Electron Microscopic ◽  
Biological Specimen ◽  
Specimen Holder ◽  
Cold Stage ◽  
Preparation Techniques ◽  
Scanning Electron

In order to observe biological specimens in their native state, that is, still containing their water content, various methods of specimen preparation have been used, the principal two of which are the chamber method and the freeze method.Using its recently developed cold stage for installation in the pre-evacuation chamber of a scanning electron microscope, we have succeeded in directly observing a biological specimen in its frozen state without the need for such conventional specimen preparation techniques as drying and metallic vacuum evaporation. (Echlin, too, has reported on the observation of surface structures using the same freeze method.)In the experiment referred to herein, a small sliced specimen was place in the specimen holder. After it was rapidly frozen by freon cooled with liquid nitrogen, it was inserted into the cold stage of the specimen chamber.

About definition of the local entropy rate of production in experiment of pulse electric heating

2020 ◽  
pp. 29-34
Author(s):  
Alexandr V. Kostanovskiy ◽  
Margarita E. Kostanovskaya
Keyword(s):  
Thermal Diffusivity ◽  
Electric Heating ◽  
Thermal Capacity ◽  
Entropy Rate ◽  
Initial Time ◽  
Local Entropy ◽  
Linear Mode ◽  
Electric Capacity ◽  
Definition Of ◽  
Pulse Electric

Work is devoted to studying of a linear mode thermodynamic – a mode which is actively investigated now. One of the main concepts of a linear mode – local entropy rate of production. The purpose of given article consists in expansion of a circle of problems for which it is possible to calculate a local entropy rate of production, namely its definition, using the experimental “time-temperature” curves of heating/cooling. “Time-temperature” curves heating or cooling are widely used in non-stationary thermophysical experiments at studying properties of substances and materials: phase transitions of the first and second sort, a thermal capacity, thermal diffusivity. The quantitative substantiation of the formula for calculation of the local entropy rate of production in which it is used thermogram (change of temperature from time) which is received by a method of pulse electric heating is resulted. Initial time dependences of electric capacity and temperature are measured on the sample of niobium in a microsecond range simultaneously. Conformity of two dependences of the local entropy rate of production from time is shown: one is calculated under the known formula in which the brought electric capacity is used; another is calculated, using the thermogram.

Biodegradable Polyurethanes Cross-Linked by Multifunctional Compounds

2017 ◽  
Vol 14 (6) ◽  
pp. 778-784 ◽  
Author(s):  
Joanna Brzeska
Keyword(s):  
Polymer Structure ◽  
Cross Linking ◽  
Plant Oils ◽  
Lignocellulosic Materials ◽  
Soft Or ◽  
Native State ◽  
Synthetic Compounds ◽  
Multifunctional Compounds ◽  
Hard Segments ◽  
Synthetic Origin

Background: Cross-linking structure of polyurethanes determines no degradability of these materials. However, introducing the hydrolysable substrates (of natural or synthetic origin) into the cross-linked polyurethanes structure makes them biodegradable. Moreover compounds (such as polycaprolactone triol, glycerin, lysine triisocyanate, etc.) that are used for polyurethane cross-linking are degraded in non-toxic products. All these kinds of compounds can be introduced into soft or hard segments via urethane bonds. Objective: The review focuses on kind of multifunctional polyols and isocyanates, and low molecular crosslinkers used for cross-linked polyurethanes obtaining. These compounds are natural substrates (in the native state or after modification) or are synthetic compounds with degradable linkages. They belong to polyesters, plant oils, proteins, saccharides, and others (e.g. lignocellulosic materials), and they are synthesized chemically or via biosynthesis by algae, plants, microorganisms, and by animals. Conclusion: Incorporation of degradable groups (such as ester moieties) into the polymer structure, and using of substrates with the structure known and metabolized by microorganisms for soft or hard segments building, facilitate degradation of cross-linked polyurethanes.

Optimal hydraulic design of an ultra-low specific speed centrifugal pump based on the local entropy production theory

2019 ◽  
Vol 233 (6) ◽  
pp. 715-726 ◽  
Author(s):  
Hucan Hou ◽  
Yongxue Zhang ◽  
Xin Zhou ◽  
Zhitao Zuo ◽  
Haisheng Chen
Keyword(s):  
Entropy Production ◽  
Centrifugal Pump ◽  
Orthogonal Design ◽  
High Energy ◽  
Geometrical Parameters ◽  
Production Theory ◽  
Local Entropy ◽  
Hydraulic Design ◽  
Low Specific Speed ◽  
Specific Speed

The ultra-low specific speed centrifugal pump has been widely applied in aerospace engineering, metallurgy, and other industrial fields. However, its hydraulic design lacks specialized theory and method. Moreover, the impeller and volute are designed separately without considering their coupling effect. Therefore, the optimal design is proposed in this study based on the local entropy production theory. Four geometrical parameters are selected to establish orthogonal design schemes including blade outlet setting angle, wrapping angle volute inlet width, and throat area. Subsequently, a 3D steady flow with Reynolds stress turbulent model and energy equation model is numerically conducted and the entropy production is calculated by a user-defined function code. The range analysis is made to identify the optimal scheme indicating that the combination of local entropy production and orthogonal design is feasible on pump optimization. The optimal pump is visibly improved with an increase of 1.08% in efficiency. Entropy production is decreased by 16.75% and 6.03% in impeller and volute, respectively. High energy loss areas are captured and explained in terms of helical vortex and wall friction, and the turbulent and wall entropy production are respectively reduced by 3.82% and 14.34% for the total pump.

scholarly journals Design of epidermal growth factor immobilization on 3D biocompatible scaffolds to promote tissue repair and regeneration

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Teodora Bavaro ◽  
Sara Tengattini ◽  
Refaya Rezwan ◽  
Enrica Chiesa ◽  
Caterina Temporini ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Tissue Repair ◽  
Peptide Mapping ◽  
Rnase A ◽  
Fibroblast Cells ◽  
Native State ◽  
Tissue Repair And Regeneration ◽  
Epidermal Growth ◽  
Cell Proliferative Activity

AbstractExogenous application of human epidermal growth factor (hEGF) stimulates epidermal wound healing. The aim of this study was to develop bioconjugates based on hEGF mimicking the protein in its native state and thus suitable for tissue engineering applications, in particular for treating skin-related disorders as burns. Ribonuclease A (RNase A) was used to investigate a number of different activated-agarose carriers: cyanogen bromide (CNBr)-activated-agarose and glyoxyl-agarose showed to preserve the appropriate orientation of the protein for receptor binding. EGF was immobilized on these carriers and immobilization yield was evaluated (100% and 12%, respectively). A peptide mapping of unbound protein regions was carried out by LC–MS to take evidence of the residues involved in the immobilization and, consequently, the flexibility and surface accessibility of immobilized EGF. To assess cell proliferative activities, 10, 25, 50, and 100 ng/mL of each immobilized EGF sample were seeded on fibroblast cells and incubated for 24, 48 and 72 h. The immobilized growth factor showed significantly high cell proliferative activity at 50 and 100 ng/mL compared to control and soluble EGF. Although both of the immobilized samples show dose-dependency when seeded with high number of fibroblast cells, CNBr-agarose-EGF showed a significantly high activity at 100 ng/mL and 72 h incubation, compared to glyoxyl-agarose-EGF.

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