scholarly journals Enhancer Trap Expression Patterns Provide a Novel Teaching Resource

2002 ◽  
Vol 130 (4) ◽  
pp. 1747-1753 ◽  
Author(s):  
Matt Geisler ◽  
Barbara Jablonska ◽  
Patricia S. Springer
Keyword(s):  
Expression Patterns ◽  
Enhancer Trap ◽  
Teaching Resource

The Drosophila mushroom body is a quadruple structure of clonal units each of which contains a virtually identical set of neurones and glial cells

Development ◽  
1997 ◽  
Vol 124 (4) ◽  
pp. 761-771 ◽  
Author(s):  
K. Ito ◽  
W. Awano ◽  
K. Suzuki ◽  
Y. Hiromi ◽  
D. Yamamoto
Keyword(s):  
Glial Cells ◽  
Sensory Integration ◽  
Mushroom Body ◽  
Expression Patterns ◽  
Cell Lineage ◽  
Cell Types ◽  
Enhancer Trap ◽  
Adult Brain ◽  
A New Technique ◽  
Lineage Analysis

The mushroom body (MB) is an important centre for higher order sensory integration and learning in insects. To analyse the development and organisation of the MB neuropile in Drosophila, we performed cell lineage analysis in the adult brain with a new technique that combines the Flippase (flp)/FRT system and the GAL4/UAS system. We showed that the four mushroom body neuroblasts (MBNbs) give birth exclusively to the neurones and glial cells of the MB, and that each of the four MBNb clones contributes to the entire MB structure. The expression patterns of 19 GAL4 enhancer-trap strains that mark various subsets of MB cells revealed overlapping cell types in all four of the MBNb lineages. Partial ablation of MBNbs using hydroxyurea showed that each of the four neuroblasts autonomously generates the entire repertoire of the known MB substructures.

scholarly journals Subdivision of the drosophila mushroom bodies by enhancer-trap expression patterns

Neuron ◽  
1995 ◽  
Vol 15 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Ming Yao Yang ◽  
J.Douglas Armstrong ◽  
Ilya Vilinsky ◽  
Nicholas J. Strausfeld ◽  
Kim Kaiser
Keyword(s):  
Expression Patterns ◽  
Enhancer Trap ◽  
Mushroom Bodies

scholarly journals High-resolution analysis of central nervous system expression patterns in zebrafish Gal4 enhancer-trap lines

2015 ◽  
Vol 244 (6) ◽  
pp. 785-796 ◽  
Author(s):  
Hideo Otsuna ◽  
David A. Hutcheson ◽  
Robert N. Duncan ◽  
Adam D. McPherson ◽  
Aaron N. Scoresby ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
High Resolution ◽  
Expression Patterns ◽  
Enhancer Trap ◽  
High Resolution Analysis ◽  
Resolution Analysis ◽  
Enhancer Trap Lines

scholarly journals A Novel Drosophila Alkaline Phosphatase Specific to the Ellipsoid Body of the Adult Brain and the Lower Malpighian (Renal) Tubule

Genetics ◽  
2000 ◽  
Vol 154 (1) ◽  
pp. 285-297 ◽  
Author(s):  
Ming Yao Yang ◽  
Zongsheng Wang ◽  
Matthew MacPherson ◽  
Julian A T Dow ◽  
Kim Kaiser
Keyword(s):  
Alkaline Phosphatase ◽  
Expression Patterns ◽  
Fluid Secretion ◽  
Regulatory Elements ◽  
Enhancer Trap ◽  
Adult Brain ◽  
P Element ◽  
Renal Tubules ◽  
Genomic Locus ◽  
Ellipsoid Body

Abstract Two independent Drosophila melanogaster P{GAL4} enhancer-trap lines revealed identical GAL4-directed expression patterns in the ellipsoid body of the brain and in the Malpighian (renal) tubules in the abdomen. Both P-element insertions mapped to the same chromosomal site (100B2). The genomic locus, as characterized by plasmid rescue of flanking DNA, restriction mapping, and DNA sequencing, revealed the two P{GAL4} elements to be inserted in opposite orientations, only 46 bp apart. Three genes flanking the insertions have been identified. Calcineurin A1 (previously mapped to 21E-F) lies to one side, and two very closely linked genes lie to the other. The nearer encodes Aph-4, the first Drosophila alkaline phosphatase gene to be identified; the more distant gene [l(3)96601] is novel, with a head-elevated expression, and with distant similarity to transcription regulatory elements. Both in situ hybridization with Aph-4 probes and direct histochemical determination of alkaline phosphatase activity precisely matches the enhancer-trap pattern reported by the original lines. Although the P-element insertions are not recessive lethals, they display tubule phenotypes in both heterozygotes and homozygotes. Rates of fluid secretion in tubules from c507 homozygotes are reduced, both basally, and after stimulation by CAP2b, cAMP, or Drosophila leucokinin. The P-element insertions also disrupt the expression of Aph-4, causing misexpression in the tubule main segment. This disruption extends to tubule pigmentation, with c507 homozygotes displaying white-like transparent main segments. These results suggest that Aph-4, while possessing a very narrow range of expression, nonetheless plays an important role in epithelial function.

Analysis of an enhancer trap expressed in regenerating Drosophila imaginal discs

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 724-736 ◽  
Author(s):  
William R. Addison ◽  
William J. Brook ◽  
Laura D. Querengesser ◽  
Stanley Y. K. Tiong ◽  
Michael A. Russell
Keyword(s):  
Expression Patterns ◽  
Imaginal Discs ◽  
Enhancer Trap ◽  
Northern Analysis ◽  
Neural Cells ◽  
Cell Fates ◽  
Imprecise Excision ◽  
Selector Genes ◽  
Sensory Neural

In Drosophila, imaginal discs are the undifferentiated larval precursors of the pattern of epidermal and sensory neural cells in each adult segment. Although cell fates are already specified by late third instar, disc fragments can either regenerate or duplicate after growth in culture. The outcome depends on signaling between cells across the healed wound and involves a redeployment of the expression patterns of selector genes and other disc pattern genes. We recently used the enhancer-trap method to screen for such genes that are expressed ectopically at the wound-heal site in imaginal discs undergoing regeneration. Here we report the cloning by plasmid rescue of transcribed sequences adjacent to one such enhancer-trap insertion. Using Northern analysis and in situ hybridization we show that one transcript is expressed in the embryo and in imaginal discs in a pattern similar to that of the enhancer trap. We also, by imprecise excision of the enhancer-trap insertion, generated a series of flanking deletions that were mapped using Southern analysis and complementation.Key words: Drosophila, imaginal discs, enhancer traps, regeneration genes.

scholarly journals A Mammalian enhancer trap resource for discovering and manipulating neuronal cell types

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Yasuyuki Shima ◽  
Ken Sugino ◽  
Chris Martin Hempel ◽  
Masami Shima ◽  
Praveen Taneja ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Neuronal Cell ◽  
Cell Types ◽  
Enhancer Trap ◽  
Marker Genes ◽  
Cell Type ◽  
Reporter Expression ◽  
Other Information ◽  
Bac Transgenesis ◽  
Cell Type Specific

There is a continuing need for driver strains to enable cell-type-specific manipulation in the nervous system. Each cell type expresses a unique set of genes, and recapitulating expression of marker genes by BAC transgenesis or knock-in has generated useful transgenic mouse lines. However, since genes are often expressed in many cell types, many of these lines have relatively broad expression patterns. We report an alternative transgenic approach capturing distal enhancers for more focused expression. We identified an enhancer trap probe often producing restricted reporter expression and developed efficient enhancer trap screening with the PiggyBac transposon. We established more than 200 lines and found many lines that label small subsets of neurons in brain substructures, including known and novel cell types. Images and other information about each line are available online (enhancertrap.bio.brandeis.edu).

Distinct expression patterns detected within individual tissues by the GAL4 enhancer trap technique

Genome ◽  
1996 ◽  
Vol 39 (1) ◽  
pp. 174-182 ◽  
Author(s):  
Kerstin Gustafson ◽  
Gabrielle L. Boulianne
Keyword(s):  
Gene Expression ◽  
Transcriptional Control ◽  
Germ Line ◽  
Expression Patterns ◽  
Cell Types ◽  
Enhancer Trap ◽  
P Element ◽  
Immunocytochemical Staining ◽  
Specific Cell ◽  
Drosophila Genome

To identify genes that are expressed in specific cell types or tissues during development, we generated enhancer-trap lines in which the yeast transcriptional activator, GAL4, was mobilized throughout the Drosophila genome. The GAL4 lines are part of a two-part system involving GAL4 and its target, the upstream activating sequence (UAS). Detection of GAL4 expression patterns was achieved by crossing individual GAL4 lines with flies carrying the reporter gene lacZ under the transcriptional control of the UAS followed by histochemical and immunocytochemical staining. Here, we present the results of this screen and the characterization of GAL4 lines that show distinct patterns of gene expression during Drosophila development, including embryogenesis, oogenesis, and imaginai disc development. However, we were unable to identify GAL4 lines that were expressed within the germ line or during early embryogenesis. Furthermore, consistent with previous results, we found that the GAL4 enhancer trap technique had a much lower frequency of transposition than has been reported for lacZ enhancer trap screens. Taken together, these results demonstrate both the strengths and weaknesses of the GAL4 enhancer trap technique for identifying unique patterns of gene expression during development. Key words : GAL4, enhancer trap, Drosophila, P element.

Functional analysis of GUS expression patterns and T-DNA integration characteristics in rice enhancer trap lines

Plant Science ◽  
2005 ◽  
Vol 168 (6) ◽  
pp. 1571-1579 ◽  
Author(s):  
Hao Peng ◽  
Hongmei Huang ◽  
Yongzhi Yang ◽  
Ying Zhai ◽  
Jinxia Wu ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Expression Patterns ◽  
Gus Expression ◽  
Enhancer Trap ◽  
Dna Integration ◽  
Enhancer Trap Lines
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