Cytotoxic efficiency of pulsed electric field treated Plectranthus amboinicus leaf extract on lung (A549) cancer cell lines

Justicia gendarussamethanolic leaf extracts from five different locations in the Southern region of Peninsular Malaysia and two flavonoids, kaempferol and naringenin, were tested for cytotoxic activity. Kaempferol and naringenin were two flavonoids detected in leaf extracts using gas chromatography-flame ionization detection (GC-FID). The results indicated that highest concentrations of kaempferol and naringenin were detected in leaves extracted from Mersing with 1591.80 mg/kg and 444.35 mg/kg, respectively. Positive correlations were observed between kaempferol and naringenin concentrations in all leaf extracts analysed with the Pearson method. The effects of kaempferol and naringenin from leaf extracts were examined on breast cancer cell lines (MDA-MB-231 and MDA-MB-468) using MTT assay. Leaf extract from Mersing showed high cytotoxicity against MDA-MB-468 and MDA-MB-231 with IC50values of 23 μg/mL and 40 μg/mL, respectively, compared to other leaf extracts. Kaempferol possessed high cytotoxicity against MDA-MB-468 and MDA-MB-231 with IC50values of 23 μg/mL and 34 μg/mL, respectively. These findings suggest that the presence of kaempferol in Mersing leaf extract contributed to high cytotoxicity of both MDA-MB-231 and MDA-MB-468 cancer cell lines.

Download Full-text

Evaluation of Anti Cancer Activity of Kydia calycina Roxb. Leaf Extract on Different Cancer Cell Lines

Research Journal of Pharmacognosy and Phytochemistry ◽

10.5958/0975-4385.2017.00036.x ◽

2017 ◽

Vol 9 (4) ◽

pp. 197

Author(s):

Baburao Bhukya ◽

Harikiran Lingabathula ◽

Narsimhareddy Yellu

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Leaf Extract ◽

Cancer Cell Lines ◽

Anti Cancer ◽

Cancer Activity

Download Full-text

Ethanolic neem (Azadirachta indica A. Juss) leaf extract induces apoptosis and inhibits the IGF signaling pathway in breast cancer cell lines

Biomedicine & Preventive Nutrition ◽

10.1016/j.bionut.2011.12.008 ◽

2012 ◽

Vol 2 (1) ◽

pp. 59-68 ◽

Cited By ~ 13

Author(s):

Perumal Elumalai ◽

Dharmalingam Nandagopal Gunadharini ◽

Kalimuthu Senthilkumar ◽

Sivanantham Banudevi ◽

Ramachandran Arunkumar ◽

...

Keyword(s):

Breast Cancer ◽

Signaling Pathway ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Azadirachta Indica ◽

Leaf Extract ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Igf Signaling

Download Full-text

Phytochemical screening and evaluation of cytotoxic activity of Calotropis gigantea leaf extract on MCF7, HeLa, and A549 cancer cell lines

Journal of Natural Science Biology and Medicine ◽

10.4103/jnsbm.jnsbm_215_18 ◽

2019 ◽

Vol 10 (2) ◽

pp. 131 ◽

Cited By ~ 1

Author(s):

Venkataraman Prabhu ◽

Bindu Damodaran ◽

Prashantha Nagaraja ◽

Vivek Jain ◽

MP Manuja Viraj Wimalasiri ◽

...

Keyword(s):

Cytotoxic Activity ◽

Cell Lines ◽

Cancer Cell ◽

Leaf Extract ◽

Cancer Cell Lines ◽

Phytochemical Screening ◽

Calotropis Gigantea

Download Full-text

In-vitro Observations of Potassium Ion Effusion and Apoptosis with the External DC Electric Field in the Breast Cancer Cell Lines

World Congress on Medical Physics and Biomedical Engineering 2006 - IFMBE Proceedings ◽

10.1007/978-3-540-36841-0_704 ◽

2007 ◽

pp. 2786-2788

Author(s):

H. B. Kim ◽

K. W. Kim ◽

J. H. Chung ◽

S. B. Sim ◽

S. Ahn

Keyword(s):

Breast Cancer ◽

Electric Field ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Cancer Cell Lines ◽

Potassium Ion ◽

Breast Cancer Cell Lines ◽

Dc Electric Field

Download Full-text

Anti-proliferative activities of Byrsocarpus coccineus using ovarian cancer cell lines

10.21203/rs.3.rs-16751/v1 ◽

2020 ◽

Author(s):

Caroline Ukwade ◽

Osaretin Ebuehi ◽

Rashidat Adisa ◽

Santos Singh ◽

Rajesh Singh

Keyword(s):

Cell Cycle ◽

Ovarian Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Leaf Extract ◽

Ovarian Cancer Cell ◽

Cancer Cell Lines ◽

Dependent Manner ◽

Inhibition Of Proliferation ◽

Ovarian Cancer Cell Lines

Abstract Background: Ovarian cancer is one of the most lethal tumors of gynecologic malignancies, due to lack of early detection, and a high rate of metastasis. The standard treatment is surgery and cytotoxic chemotherapy, but due to its cost and side effects, medicinal plants are widely used in developing countries. Byrsocarpus coccineus plant preparation, has been administered to patients traditionally in the management of tumour in Nigeria. In this study, we investigated the anti-proliferative effects of B. coccineus ethanol leaf extract against OVCAR3 and SW626 ovarian cancer cell lines. After treatment of the two cell lines with the extracts, analyses were carried out to determine inhibition of proliferation and expression of cell cycle markers, pro-apoptotic and anti-apoptotic markers. Results: Results showed that B. coccineus ethanol leaf extract, significantly inhibited cell migration and colony formation in OVCAR3 and SW626 treated cells in a dose-dependent manner. Results also show that B. coccineus ethanol leaf extract modulated the expression of p53 gene, cell cycle progression, anti-apoptotic and pro-apoptotic genes. Conclusions: These results suggest that B. coccineus have anti-proliferative properties and could induce apoptosis. Further investigation will be carried out to isolate bioactive compounds for the treatment of ovarian cancer.

Download Full-text