We present a lab-on-a-chip for the next generation of single-cell genomics, performing full-cycle single-cell analysis by demonstrating mega-base pair genomic DNAs in nanochannels extracted in situ.
Abstract
Developing the next generation of scanning helium microscopes requires the fabrication of optical elements with complex internal geometries. We show that resin stereolithography (SLA) 3D printing produces low-cost components with the requisite convoluted structures whilst achieving the required vacuum properties, even without in situ baking. As a case study, a redesigned pinhole plate optical element of an existing scanning helium microscope was fabricated using SLA 3D printing. In comparison to the original machined component, the new optical element minimised the key sources of background signal, in particular multiple scattering and the secondary effusive beam.
A quadrupole mass spectrometer unit was utilized to accurately detect the chemical
species present inside a SiC CVD reactor growth chamber before, during, and after epitaxial
deposition. The in-situ mass spectrometer has been able to confirm the presence of silane (SiH4)
and propane (C3H8) decomposition products (eg. Si and CH4) that were predicted from chemical
modelling, and give insight into specific reaction kinetics. Additionally, the mass spectrometer has
positively detected trace amounts of oxygen, which has helped to identify process weaknesses and
possible sources of vacuum leaks.
In-situ gamma-ray background measurements for next generation CDEX experiment in the China Jinping Underground Laboratory
