scholarly journals Efficacy of CNC-diode laser combine with chlorophylls to eliminate staphylococcus aureus biofilm

2016 ◽  
Author(s):  
Suryani D. Astuti ◽  
Deni Arifianto ◽  
Nike D. G. Drantantiyas ◽  
Aulia M. T. Nasution ◽  
Abdurachman
Keyword(s):  
Staphylococcus Aureus ◽  
Diode Laser

scholarly journals Efficacy of titanium brush, 915 nm diode laser, citric acid for eradication of Staphylococcus aureus from implant surfaces

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mohammad Reza Karimi ◽  
Behshad Farkhondemehr ◽  
Motahare Ghaeni Najafi ◽  
Ardavan Etemadi ◽  
Nasim Chiniforush
Keyword(s):  
Staphylococcus Aureus ◽  
Citric Acid ◽  
Diode Laser ◽  
Acid Group ◽  
Negative Control ◽  
Control Groups ◽  
Implant Surface ◽  
Mini Implants ◽  
Significant Difference ◽  
Post Hoc

Abstract Background This study aimed to assess the efficacy of titanium brush, 915 nm diode laser, citric acid and the combination of latter two with titanium brush for decontamination of SLA surface mini-implants. Methods Seventy-five mini-implants contaminated with Staphylococcus aureus (S. aureus) were randomly divided into five experimental groups (n = 12) of titanium brush (TiB), laser, citric acid (CA), brush-laser, and brush-acid, positive [n = 12; chlorhexidine mouthwash (CHX)] and negative [n = 2; phosphate buffered saline (PBS)] control groups and one no-treatment group (n = 1). After counting the colony forming units (CFUs), data were analyzed using the Kruskal–Wallis and Dunn post-hoc tests. Results Regardless of the no-treatment and negative control groups, maximum and minimum CFUs were noted in the titanium brush and positive control groups. After CHX, minimum CFUs were noted in brush-acid group followed by brush-laser, laser, and acid groups. Generally, the Kruskal–Wallis test revealed a significant difference between the groups regarding the colony count (P < 0.001). Dunn post-hoc test showed that the difference between the titanium brush and acid-brush group was significant (P < 0.001) while the differences between the brush and laser groups with the brush-laser group were not significant (P > 0.077). Conclusions Combined use of titanium brush and citric acid yielded superior results compared to other groups in reduction of S. aureus on implant surface.

scholarly journals The efficacy of photodynamic inactivation with laser diode on Staphylococcus aureus biofilm with various ages of biofilm

2020 ◽  
Author(s):  
Suryani Dyah Astuti ◽  
Hafidiana ◽  
Riries Rulaningtyas ◽  
Abdurachman ◽  
Alfian P Putra ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Energy Density ◽  
Diode Laser ◽  
Laser Diode ◽  
Elisa Test ◽  
Control Group ◽  
Photodynamic Inactivation ◽  
Bacterial Cell Death ◽  
Irradiation Energy ◽  
Blue Diode Laser

Biofilms are able to casue microorganisms to be 80% more resistant to antibiotics. The extracelullar polymeric substance (EPS) in biofilm functions to protect bacteria, making it difficult for antibiotics to penetrate the biofilm layer. This study aims to determine the effective- ness of photodynamic inactivation with blue diode laser to reduce Staphylococcus aureus biofilm at various ages of biofilms. The light source is a 403 nm blue diode laser with an energy power of about 27.65±0.01 mW. The study was designed with two groups: Group C was the untreated control group with variations in age of biofilms (0; 6; 11; 17; 24; 32; 40 and 48) hours; Group T was a laser treatment group with variations in age of biofilm and energy density (4.23; 8.46; 12.70; 16.93 and 21.16) J/cm2. Biofilm reduction measurement method using ELISA test was performed to calculate OD595 value. The statistical analysis results of variance showed that there was an influence of biofilm age and irradiation energy density of laser on biofilm reduction. Optical density analysis showed the most optimum biofilm reduction happened when biofilm age is perfectly constructed (about 17 hours) and with 91% reduction. The longer biofilm age lived among those biofilms, the greater the reduction. The results of the Scanning Microscope Electron and fluorescent microscope measurement showed destruction site of the EPS biofilm and bacterial cell death. So, the activated photodynamic with 403 nm laser diode is effective to reduce the Staphylococcus aureus biofilm in the maturation phase.

scholarly journals Antimicrbial Effect of Diode Laser and Biossynthesis Silver Nanoparticles on Bactrium Staphylococcus Aureus in Vitro

2018 ◽  
Vol 7 (4.36) ◽  
pp. 290
Author(s):  
Raad Sh. Alnayli ◽  
Adnan H. Al Hamadani ◽  
Safaa A. Yaseen
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Diode Laser ◽  
Bacterial Growth ◽  
Antibacterial Agent ◽  
Laser Energy ◽  
Nutrient Broth ◽  
Biological Method ◽  
Effective Result

The present work was aimed to illustrate how the use of Diode laser or silver nanoparticles (AgNPs) each of them individually, as an antibacterial agent against  Staphylococcus aureus (S. aureus) as well as study the effective result from the absorption of laser energy by these nanoparticles to kill or inhibition bacterial growth. The silver nanoparticles are prepared by biological method. S. aureus are isolated and identified in the Central Health Laboratory in Al Najaf city. Nanoparticles are tested against S. aureus cultured on Muller Hinton agar but Diode laser or laser with nanoparticles are tested against S. aureus cultured in Nutrient broth. S. aureus are irradiating by Diode laser with different irradiation times, moreover there are different concentrations of AgNPs have been employed for killing and inhibition bacterial growth. Results showed that S. aureus is affected by AgNPs (mainly highest concentration of nanoparticles) also detected that decrease bacterial availability with increase time of irradiation to laser. 

The effects of lasers and nanoparticles to kill and inactivate bacteria

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Huda K Mohsen ◽  
Sadiq H Lafta ◽  
Qaiser N Mathlum
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Diode Laser ◽  
Modern Method ◽  
Biological Science ◽  
Absorption Property ◽  
Gram Positive ◽  
Gold And Silver Nanoparticles ◽  
Gram Positive Bacteria ◽  
Conventional Antibiotics

The laser and nano-biological technical, representing the concourse biological science and nanotechnology,is considered modern method used as an anti-bacterial instead of conventional antibiotics. The present study is an attempt to illustrate how the use of lasers and silver nanoparticles each of them individually, as an anti-bacterial against the Staphylococcus aureus which gram positive bacteria. Bacteria (S. aureus) are irradiating by Nd: YVO4 and Diode laser with different powers and wavelengths,gold and silver nanoparticles it has been employed in the killing and inhibition of bacteria,by the effect of nanoparticles on its own,as well as using the absorption property of these nanoparticles for laser and subsequent mechanism this feature interact with bacteria.

scholarly journals Inactivation of Staphylococcal Virulence Factors Using a Diode Laser light-Activated Antimicrobial Agent

2015 ◽  
Vol 9 (2) ◽  
pp. 94-101
Author(s):  
Norrya A. Ali ◽  
Dheyaa Khaleel Ismael ◽  
Rafeef Yousif Rasheed
Keyword(s):  
Staphylococcus Aureus ◽  
Diode Laser ◽  
Virulence Factors ◽  
Laser Light ◽  
Meca Gene ◽  
Antibiotic Sensitivity ◽  
Toxin Production ◽  
Confirmatory Test ◽  
Before And After ◽  
Titration Assay

Total of 120 samples were collected from different body sites and lesions for patients from bothsexes. There were 78 isolates (65%) Staphylococcus aureus from them 37 isolates were Methicillinresistant Staphylococcus aureus (MRSA). Each isolate was exposed to diode laser 632nmwavelength with 300µM of methylene blue (MB) at 458.6 watt/cm2for 15 minutes, bothphenotypic and genotypic changes in virulence factors and the antibiotic-resistance wereevaluated before and after irradiation with laser light. It was observed that the effect of sub-lethaldose on the antibiotic sensitivity was isolate-dependent. In general, results showed large variationsin the susceptibility of antibiotic. Exposure to diode laser increased resistance to Vancomycin, incontrast increased sensitivity to Methicillin, Ciprofloxacin, and Norfloxacin. Also Results showeddecreases the activity of β-haemolysin, with 33 (90.3%) isolates of S. aureus in comparison to thecontrol as shown in blood agar method and haemolytic titration assay, but there was no effect onthermonuclease enzyme after irradiation. Detection of three genes represented in MRSA isolatesby a confirmatory test was carried out using PCR technique. The results of the PCR amplificationof mecA gene noted that it was present in 27(72.2%) S.aureus isolates and 14(83.3%) isolatesshowed reduction in β-toxin production after exposure to laser light, with no altered or deficiencyin thermonuclease gene (nuc).

scholarly journals Efficacy of Titanium Brush, 915 nm Diode Laser, Citric Acid for Eradication of Staphylococcus Aureus from Implant Surfaces

2021 ◽  
Author(s):  
Mohammad Reza Karimi ◽  
Behshad Farkhondemehr ◽  
Motahare Ghaeni Najafi ◽  
Ardavan Etemadi ◽  
Nasim Chiniforush
Keyword(s):  
Staphylococcus Aureus ◽  
Citric Acid ◽  
Diode Laser ◽  
Positive Control ◽  
Colony Count ◽  
Control Group ◽  
Control Groups ◽  
Mini Implants ◽  
Significant Difference ◽  
Post Hoc

Abstract Background: This study aimed to assess the efficacy of titanium brush, 915 nm diode laser, citric acid and the combination of latter two with titanium brush for decontamination of SLA surface mini-implants.Materials and Methods: Seventy-five mini-implants contaminated with Staphylococcus aureus (S. aureus) were randomly divided into five experimental groups (n=12) of titanium brush (TiB), laser, citric acid (CA), brush-laser, and brush-acid, positive [n=12; chlorhexidine mouthwash (CHX)] and negative [n=2; phosphate buffered saline (PBS)] control groups and one no-treatment group (n=1). After counting the colony forming units (CFUs), data were analyzed using the Kruskal-Wallis and Dunn post-hoc tests.Results: Regardless of the no-treatment and negative control groups, maximum and minimum CFUs were noted in the titanium brush and positive control groups. After CHX, minimum CFUs were noted in brush-acid group followed by brush-laser, laser, and acid groups. Generally, the Kruskal-Walli test revealed a significant difference between the groups regarding the colony count (P<0.001). Dunn post-hoc test showed that the difference between the titanium brush and acid-brush group was significant (P<0.001) while the differences between the brush and laser groups with the brush-laser group were not significant (P>0.077).Conclusions: Maximum reduction in colony count was noted in the positive control group. Combination of titanium brush and laser was also effective for decreasing the colony count; however, combined use of titanium brush and citric acid yielded superior results compared to other groups.

Cytochemical Studies of Cell Wall Biosynthesis in Staphylococcus Aureus

1972 ◽  
Vol 30 ◽  
pp. 328-329
Author(s):  
Masaatsu Koike ◽  
Koichi Nakashima ◽  
Kyoko Iida
Keyword(s):  
Staphylococcus Aureus ◽  
Periodate Oxidation ◽  
Early Time ◽  
The Other ◽  
Penicillin G ◽  
Cell Wall Biosynthesis ◽  
Septal Wall ◽  
Peptidoglycan Biosynthesis ◽  
Gram Positive Bacteria ◽  
Cross Linkage

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).

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