Efficacy of titanium brush, 915 nm diode laser, citric acid for eradication of Staphylococcus aureus from implant surfaces

Abstract Background This study aimed to assess the efficacy of titanium brush, 915 nm diode laser, citric acid and the combination of latter two with titanium brush for decontamination of SLA surface mini-implants. Methods Seventy-five mini-implants contaminated with Staphylococcus aureus (S. aureus) were randomly divided into five experimental groups (n = 12) of titanium brush (TiB), laser, citric acid (CA), brush-laser, and brush-acid, positive [n = 12; chlorhexidine mouthwash (CHX)] and negative [n = 2; phosphate buffered saline (PBS)] control groups and one no-treatment group (n = 1). After counting the colony forming units (CFUs), data were analyzed using the Kruskal–Wallis and Dunn post-hoc tests. Results Regardless of the no-treatment and negative control groups, maximum and minimum CFUs were noted in the titanium brush and positive control groups. After CHX, minimum CFUs were noted in brush-acid group followed by brush-laser, laser, and acid groups. Generally, the Kruskal–Wallis test revealed a significant difference between the groups regarding the colony count (P < 0.001). Dunn post-hoc test showed that the difference between the titanium brush and acid-brush group was significant (P < 0.001) while the differences between the brush and laser groups with the brush-laser group were not significant (P > 0.077). Conclusions Combined use of titanium brush and citric acid yielded superior results compared to other groups in reduction of S. aureus on implant surface.

Download Full-text

Efficacy of Titanium Brush, 915 nm Diode Laser, Citric Acid for Eradication of Staphylococcus Aureus from Implant Surfaces

10.21203/rs.3.rs-408196/v1 ◽

2021 ◽

Author(s):

Mohammad Reza Karimi ◽

Behshad Farkhondemehr ◽

Motahare Ghaeni Najafi ◽

Ardavan Etemadi ◽

Nasim Chiniforush

Keyword(s):

Staphylococcus Aureus ◽

Citric Acid ◽

Diode Laser ◽

Positive Control ◽

Colony Count ◽

Control Group ◽

Control Groups ◽

Mini Implants ◽

Significant Difference ◽

Post Hoc

Abstract Background: This study aimed to assess the efficacy of titanium brush, 915 nm diode laser, citric acid and the combination of latter two with titanium brush for decontamination of SLA surface mini-implants.Materials and Methods: Seventy-five mini-implants contaminated with Staphylococcus aureus (S. aureus) were randomly divided into five experimental groups (n=12) of titanium brush (TiB), laser, citric acid (CA), brush-laser, and brush-acid, positive [n=12; chlorhexidine mouthwash (CHX)] and negative [n=2; phosphate buffered saline (PBS)] control groups and one no-treatment group (n=1). After counting the colony forming units (CFUs), data were analyzed using the Kruskal-Wallis and Dunn post-hoc tests.Results: Regardless of the no-treatment and negative control groups, maximum and minimum CFUs were noted in the titanium brush and positive control groups. After CHX, minimum CFUs were noted in brush-acid group followed by brush-laser, laser, and acid groups. Generally, the Kruskal-Walli test revealed a significant difference between the groups regarding the colony count (P<0.001). Dunn post-hoc test showed that the difference between the titanium brush and acid-brush group was significant (P<0.001) while the differences between the brush and laser groups with the brush-laser group were not significant (P>0.077).Conclusions: Maximum reduction in colony count was noted in the positive control group. Combination of titanium brush and laser was also effective for decreasing the colony count; however, combined use of titanium brush and citric acid yielded superior results compared to other groups.

Download Full-text

The effect of different final irrigation solutions on apical impermeability

International Dental Research ◽

10.5577/intdentres.2021.vol11.suppl1.28 ◽

2021 ◽

Vol 11 (Suppl. 1) ◽

pp. 201-205

Author(s):

Gizem Akın Tartuk ◽

Sadullah Kaya ◽

Özkan Adıgüzel

Keyword(s):

Citric Acid ◽

Phytic Acid ◽

Ethylenediaminetetraacetic Acid ◽

Acid Group ◽

Negative Control ◽

Gutta Percha ◽

Nail Polish ◽

Mechanical Preparation ◽

Significant Difference ◽

Apical Leakage

Aim: This study aimed to compare ethylenediaminetetraacetic acid (EDTA), phytic acid, and citric acid as chelation agents in final irrigation procedures on teeth with apical impermeability. Methodology: We used 66 mandibular premolar teeth with a single root and single canal extracted for periodontal or orthodontic reasons. All teeth crowns were removed to achieve a 14-mm root length. Mechanical preparation of the root canal was completed with a Reciproc R25 (VDW, Munich, Germany) file system. Teeth were randomly separated into three groups: Group 1: 5 ml 17% EDTA, Group 2: 5 ml 1% phytic acid, and Group 3: 5 ml 20% citric acid. We used 6 ml 5.25% sodium hypochlorite (NaOCl) in the mechanical preparation of all groups. Three teeth were selected for both the positive and negative control groups. All teeth in the experimental groups were filled by the lateral condensation method using AH Plus canal sealer and gutta-percha. For hardening of the canal sealer, samples were kept at 37 oC and at 100% humidity in a drying oven for 7 days. Later, following removal from the drying oven, two layers of nail polish were used to cover all but the apical 2 mm of each tooth. The teeth were then kept in 2% methylene blue solution at 37 oC for 7 days. The root was divided by cutting in the buccolingual direction and gutta-percha was removed. The dye leakage was measured linearly in millimeters (mm) under a stereomicroscope. Results: As a result of the apical leakage assessment, the lowest apical leakage is observed in the phytic acid group. There was a statistically significant difference between the phytic acid group and citric acid group. There was no statistically significant difference between other groups. Conclusion: While phytic acid showed similar results with EDTA on apical impermeability, it was more effective than citric acid. How to cite this article: Akın Tartuk G, Kaya S, Adıgüzel Ö. The effect of different final irrigation solutions on apical impermeability. Int Dent Res 2021;11(Suppl.1):201-5. https://doi.org/10.5577/intdentres.2021.vol11.suppl1.28 Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

Download Full-text

Survival of Macrobrachium amazonicum embryos submitted to cooling

Zygote ◽

10.1017/s0967199417000119 ◽

2017 ◽

Vol 25 (3) ◽

pp. 288-295 ◽

Cited By ~ 2

Author(s):

Arthur Vinícius Lourenço Ferreira ◽

Moisés Fernandes Martins ◽

Míriam Luzia Nogueira Martins de Sousa ◽

Aldeney Andrade Soares Filho ◽

Célia Maria de Souza Sampaio

Keyword(s):

Survival Rate ◽

Storage Time ◽

Survival Rates ◽

Negative Control ◽

Control Groups ◽

Treatment Groups ◽

Significant Difference ◽

Macrobrachium Amazonicum ◽

Cryoprotectant Solution ◽

Positive Controls

SummaryCooling techniques have several applications for reproduction in aquaculture. However, few studies have sought to create protocols for cooling and cryopreservation of Macrobrachium amazonicum embryos. Thus, the objective of this work was to verify the survival of M. amazonicum embryos and the correlation between embryonic volume and mortality of M. amazonicum embryos after cooling. Embryo pools were collected from three females and divided into two treatment groups: dimethyl sulfoxide (DMSO) 3% and ethylene glycol (EG) 0.5%, both of them associated with 2 M sucrose. Positive and negative control groups consisted of seawater 10%. Aliquots of 10 µg of embryos were placed in Falcon® tubes containing a cryoprotectant solution and submitted directly to the test temperature of 2°C for 2 and 6 h of cooling. Further analysis of survival and embryonic volume were performed under a stereoscopic microscope. Data were subjected to analysis of variance (ANOVA), and means were compared using the Tukey test at 5%. The highest embryonic survival rate was observed after the shortest storage time for both the DMSO 3% and the 0.5% EG groups, with survival rates of 84.8 ± 3.9 and 79.7 ± 2.8%, respectively. There was a reduction in survival after 24 h, with the DMSO 3% group presenting a survival rate of 71.7 ± 6.6%, and the EG 0.5% group, 66 ± 6.9%. Survival showed a statistically significant difference when compared with the positive controls after 2 h and 24 h of cooling, with 99 ± 0.5% and 95.8 ± 1.5% survival rates, respectively. There was no significant statistical difference in the embryonic volume, but it was possible to observe a change in the appearance of the embryos, from a translucent coloration to an opaque white or brownish coloration, after 24 h in incubators. Thus, it can be concluded that survival is inversely proportional to storage time and that, although there was no change in the embryonic volume after cooling, a change in the appearance of embryos could be observed.

Download Full-text

Effect of Different Intermediary Bases on Microleakage of a Restorative Material in Class II Box Cavities of Primary Teeth

The International Journal of Artificial Organs ◽

10.5301/ijao.5000566 ◽

2017 ◽

Vol 40 (2) ◽

pp. 82-87 ◽

Cited By ~ 1

Author(s):

Faika Y. Abdelmegid ◽

Fouad S. Salama ◽

Waleed M. Al-Mutairi ◽

Saud K. Al-Mutairi ◽

Sultan O. Baghazal

Keyword(s):

Primary Teeth ◽

In Vitro Study ◽

Class Ii ◽

Positive Control ◽

Negative Control ◽

The Other ◽

Control Groups ◽

Significant Difference ◽

The Mean

Introduction The aim of this in vitro study was to assess and compare the effect of different intermediary bases on microleakage between tooth and a nanocomposite interface in Class II box cavities in primary teeth. Methods Standard Class II box cavities were prepared in 52 primary molars and randomly divided into 9 groups according to the intermediary base used (Multicore Flow, Fuji II LC, SDR, Smart Dentin Replacement, and Biodentine). All specimens were subjected to thermocycling and prepared for microleakage testing and evaluation. Results There was significant difference in the mean ranks of microleakage between the 9 groups, which was observed in the gingival side (p<0.0001) and the occlusal side (p<0.0001). The mean ranks microleakage was significantly higher with experimental SDR, experimental Multicore Flow, and positive control materials when compared with the other 6 groups. The microleakage mean ranks were statistically significantly lower in experimental Fuji II LC, experimental Biodentine, and all negative control groups when compared with the other 3 groups. Conclusions Microleakage is affected by the application of intermediate material. Experimental Biodentine and Fuji II LC showed the lowest microleakage while experimental SDR and experimental Multicore Flow showed the highest microleakage.

Download Full-text

Histopathological Profile of Therapeutic Doses of Mango Mistletoe Methanolic Extract (MMME) in Cardiac of Hypertensive Rats (DOCA-Salt)

JSMARTech ◽

10.21776/ub.jsmartech.2021.002.02.48 ◽

2021 ◽

Vol 2 (2) ◽

pp. 048-054

Author(s):

Annisatul Hakimah ◽

◽

Nour Sjakoer ◽

Nurul Mubarakati ◽

◽

...

Keyword(s):

Methanolic Extract ◽

Muscle Cells ◽

Positive Control ◽

Negative Control ◽

P Value ◽

Hypertensive Rats ◽

Control Groups ◽

Significant Difference ◽

Heart Muscle Cells ◽

And Control

This study determined the effect of Mango mistletoe Methanolic Extract (MMME) on the cardiac's histopathological profile of hypertensive rats (DOCA-Salt) treated. The MMME was treated on fifty rats for 14 days, divided into five treatments: negative control, positive control, doses 50, 100, and 200 mg/kg BW with five replications. One-way ANOVA analysis was used, namely JAMOVI with version 1.1.9.0, and for cell calculation, diameter using the IMAGE J application. Results showed that there were no significant differences in the histopathological profile of the cardiac in hypertensive rats (DOCA-Salt) treated with MMME for 14 days on the diameter of the heart muscle cells between the control (+) and control groups (-), treatments 1, 2, and 3. This is evidenced by the analysis of p-value> 0.05, namely 0.187 millimeters. Therefore, we concluded that MMME does not affect the diameter of heart/cardiac organ muscle cells. However, there was a significant difference in the amount of necrosis in the cardiac of hypertensive rats between control (+) and control (-) groups, treatments 1, 2, and 3. Based on the results, MMME reduces the number of necrosis in the heart/cardiac organ.

Download Full-text

Pengaruh Ozonated Water sebagai Antiseptik dalam Menghambat Pertumbuhan Staphilococcus Aureus (in vitro)

Majalah Kedokteran Gigi Indonesia ◽

10.22146/majkedgiind.15647 ◽

2012 ◽

Vol 19 (1) ◽

pp. 25

Author(s):

Yulita Kristanti ◽

D. Dessy

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Effect ◽

Negative Control ◽

Strong Negative Correlation ◽

Group I ◽

Significant Difference ◽

Group Ii ◽

One Way Anova ◽

Ozonated Water

Latar Belakang. Penelitian seputar ozonazed water akhir-akhir ini cukup banyak diminati karena bahan ini mempunyai potensi antibakteria yang menjanjikan. Tujuan Penelitian ini adalah untuk mengetahui pengaruh ozonated water pada pertumbuhan bakteri Staphylococcus Aureus. Metode Penelitian. Penelitian ini dilakukan dengan cara mencampurkan 1 ml larutan bakteri 106 CFU/ml dengan 10 ml ozonated water konsentrasi 4 ppm selama 10 detik (kelompok I), 20 detik (kelompok II), 30 detik (kelompok III), dan 40 detik (kelompok IV), akuades (kontrol negatif) kemudian semua diencerkan dengan akuabides 10ml. selanjutnya dari masing-masing kelompok diambil 0,01 ml untuk ditanam pada MHA, inkubasi 24 jam pada suhu 370 C dalam anaerobic jar kemudian dilakikan perhitungan koloni. Data dianalisis dengan Avana satu jalur dilanjutkan dengan uji LSD. Hasil. Avana satu jalur memperlihatkan adanya perbedaan yang bermakna antar kelompok perlakuan. Uji LSD menunjukkan perbedaan rerarta yang bermakna (p<0,05) antar kelompok I, II, III, IV dengan kelompok V (akuedes) terdapat perbedaan rerata yang bermakna antara kelompok I dan kelompok VI (iod). Tidak terdapat perbedaan bermakna antara kelompok II, III, IV terdapat kelompok VI (oid). Uji korelasi Produk Momen Pearson menunjukkan adanya korelasi negatif yang sangat kuat. Kesimpulan. Ozonated Water 4 ppm memiliki daya antibakreri dalam menghambat pertumbuhan Staphylococcus Aureus. Semakin lama waktu kontak, daya anti bakterinya semakain besar. Background. Some previous research show that the antibacterial effect of ozonated water is very promi-sing. The aim of this study was to know the antibacterial effect of ozonated water on staphylococcus aureus. Method. One ml of 106 CFU/ ml S. aureus suspension was mixed with 10 ml ozonated water for 10 second (group I), 20 second (group II), 30 second (group III), 40 second (group IV)As negative control S. aureus was mixed with aquadest (group V), and as positive control S. aureus was mixed with iod (groupVI). Furthermore, 0,01 ml from each group was cultivated on MHA, incubated for 24 hours-37C followed by colony caunting. Data was analyzed using one way anova followed by LSD. Result. One way anova show significant difference among the group and LSD test show significant mean different between group I, II, III, IV, and V. Significant difference can also be seen between group I and V. No significant difference between group II, III, IV and VI. Product momen test show strong negative correlation. Conclusion. Ozonated water 4 ppm has antibacterial effect on S. aureus. The longer the contact time, the stronger the antibacterial effect.

Download Full-text

The Effect of Strontium Ranelate Gel on Bone Formation in Calvarial Critical Size Defects

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2017.164 ◽

2017 ◽

Vol 5 (7) ◽

pp. 994-999 ◽

Cited By ~ 2

Author(s):

Hani El Nahass ◽

Nada Nour El Din ◽

Sherine Adel Nasry

Keyword(s):

Bone Formation ◽

Strontium Ranelate ◽

Cost Effective ◽

Negative Control ◽

Female Rats ◽

Control Groups ◽

Critical Size Defects ◽

Cost Effective Method ◽

Significant Difference ◽

Median Area

AIM: The current study was designed to investigate the effectiveness of locally applied Strontium ranelate to induce bone formation.MATERIALS AND METHODS: Forty-eight female rats were divided into six groups (eight rats in each group): The three test groups included Strontium (SR) 2.5 mg, 5 mg and 10 mg that was dissolved in methylcellulose gel. The control groups included methylcellulose, simvastatin 5 mg and a negative control where the defect was left to heal without any intervention. At 44 days the groups were sacrificed, and the bone defects were assessed histomorphometically to assess bone formation. The data was statistically analysed.RESULTS: There was a statistically significant difference in the amount of new bone formation between all groups, where the 2.5 mg SR group showed the highest median bone percentage, is 41.95 %, followed by the 5, and 10 mg SR demonstrating a median bone are a percentage of 39.89%, and 30.19% respectively. Simvastatin showed a median bone percentage of 36.07 %, while the methylcellulose and the negative control groups demonstrated the lowest median area percentage of 23.12 and 20.70 % respectively.CONCLUSIONS: The study showed that the local application of an SR could up-regulate the bone formation and may prove to be a cost-effective method of bone regeneration.

Download Full-text

Low-Level Laser Therapy with a 635 nm Diode Laser Affects Orthodontic Mini-Implants Stability: A Randomized Clinical Split-Mouth Trial

Journal of Clinical Medicine ◽

10.3390/jcm9010112 ◽

2019 ◽

Vol 9 (1) ◽

pp. 112 ◽

Cited By ~ 9

Author(s):

Rafał Flieger ◽

Tomasz Gedrange ◽

Kinga Grzech-Leśniak ◽

Marzena Dominiak ◽

Jacek Matys

Keyword(s):

Total Energy ◽

Laser Irradiation ◽

Diode Laser ◽

Control Group ◽

Laser Application ◽

Pain Level ◽

Mini Implants ◽

Attached Gingiva ◽

Significant Difference ◽

Secondary Stability

Background: The study aimed to clinically estimate an influence of a 635 nm diode laser on the stability of orthodontic mini-implants, to assess mini-implants loss, and to evaluate a pain level after the treatment. Materials and Methods: The randomized clinical split-mouth trial included 20 subjects (13 women and 7 men; age: 32.5 ± 6.1 years), 40 implants (RMO, West Colfax Ave., Denver, CO, USA) with a diameter 1.4 mm and length of 10 mm. Mini-implants were placed in the area of the attached gingiva between the second premolar and first molar teeth, 2 mm below the mucogingival junction of both sides of the maxilla. Each implant on the right side (G1, n = 20) of the maxilla was irradiated with a diode laser, and the implants on the opposite side (left, G2, n = 20) were a control group (without laser irradiation). The 635-nm laser parameters; dose: 10 J per point (20 J/cm2), time: 100 s per point, two points (irradiation on a buccal, and a palatal side of the alveolus/implant), the total energy per session 20 J. Laser application protocol: immediately and 3, 6, 9, 12, 15, and 30 days after surgery. The total energy after all therapeutic sessions was 140 J. The implants’ stability was measured employing a Periotest device (Periotest Test Value—PTV) immediately and 3, 6, 9, 12, 15, 30, and 60 days after the insertion of the mini-implants. Results: We found significantly higher secondary stability, lower mean PTV (6.18 ± 5.30) and (1.51 ± 2.25), for self-drilling mini-implants (G1, test group) in contrast to the control, G2 group (9.17 ± 8.25) and (5.00 ± 3.24), after 30 (p = 0.0003) and 60 days (p = 0.0000). Moreover, the analysis of the mini-implants stability after 635-nm diode laser application revealed significant higher stability in comparison with none irradiated implants (G2 group) after 3 days. (p = 0.0000) There was no significant difference in pain level measured on the NRS-11 scale on both sides of the maxilla. (p = 0.3665) An important finding was that all inserted mini-implants survived during a two-month observation period. Conclusions: 635-nm diode laser at laser irradiation increases the secondary stability of orthodontic mini-implants.

Download Full-text

Effect of Liquid Polishing System on Color Stability of Bleached Enamel

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.804.141 ◽

2013 ◽

Vol 804 ◽

pp. 141-145

Author(s):

Xiu Lan Chen ◽

Huang Qin Chen

Keyword(s):

Red Wine ◽

Color Change ◽

Color Stability ◽

Control Groups ◽

Significant Difference ◽

Before And After ◽

Total Color Change ◽

Post Hoc ◽

And Control ◽

One Way Anova

The purpose of this study was to investigate the effect of liquid polishing system (BisCover) on color stability of the bleached enamel upon exposure to a staining agent-red wine. Forty specimens were prepared. They were divided into 2 groups and different procedures, including only bleaching with Opalescence PF (PF) or Opalescence regular (R) gels, and application of BisCover after blaeching (PF-B, R-B) were used. Unbleached specimens were served as control groups (control-1 and control-2). The specimens were stored for 48 h in red wine. The color of all specimens was measured before and after exposure with a spectrophotometer, and total color change (ΔE*) were calculated. The data were analyzed with a one-way ANOVA and the means were compared by Tukeys post hoc analysis (α=0.05). PF-B and R-B groups demonstrated significantly less color change than PF and R groups. the control-1 and control-2 groups had the least color change. No significant difference were observed between PF and R group. BisCover improved the color stability of bleached enamel in red wine.

Download Full-text

The In Situ Effect of Titanium Tetrafluoride Gel on Erosion/Abrasion Progression in Human Dentin

Brazilian Dental Journal ◽

10.1590/0103-6440201601247 ◽

2017 ◽

Vol 28 (3) ◽

pp. 337-345

Author(s):

Taís Fonseca Mantilla ◽

Cecilia Pedroso Turssi ◽

Thayanne Monteiro Ramos-Oliveira ◽

Camila Vieira da Silva ◽

Luis Cláudio Suzuki ◽

...

Keyword(s):

Citric Acid ◽

Repeated Measures ◽

Environmental Scanning Electron Microscopy ◽

Control Groups ◽

Titanium Tetrafluoride ◽

Surface Loss ◽

Significant Difference ◽

Human Dentin

Abstract Erosion incidence is increasing and its control is still a challenge in clinical practice. This study evaluated 4% TiF4-gel effects on eroded human dentin subjected to in situ erosive/abrasive episodes. Seventy-two previously eroded dentin slabs (0.05 M citric acid, pH 2.3, 20 min) were allocated to 6 groups (n=12) according to the treatment to be performed during the in situ phase and number of erosive/abrasive cycles, as follows: 4% TiF4-gel applied once (TiF41), twice (TiF42) or three times (TiF43) followed by 1, 2 and 3 erosive/abrasive cycles, respectively. Gel was applied before the beginning of the next cycle. Control groups were subjected to 1 (C1), 2 (C2) and 3 (C3) erosive/abrasive cycles only. A seventh group (n=12) comprised in vitro uneroded samples (UN) subjected to 3 erosive/abrasive cycles. Each cycle corresponded to 2 days of erosive (citric acid 0.5%, pH 2.6, 6x/day) and abrasive (electric toothbrush, 10 s/sample, 1 x/day) challenges. Samples were evaluated under profilometry and environmental scanning electron microscopy (ESEM). Atomic force microscopy images (AFM) were also made (n=3). Repeated measures 2-way ANOVA and Tukey test (p<0.001) showed that TiF42, which did not differ from TiF41 and TiF43, revealed a significant reduction in surface loss compared to all control groups. TiF41 and TiF43 showed no significant difference from C1, but both groups demonstrated significantly smaller surface loss than C2 and C3. ESEM and AFM micrographs suggested alterations on treated surfaces compared to samples from control groups, showing reduced diameters of dentinal tubules lumens. Therefore, TiF4 was able to reduce the progression of erosive/abrasive lesions.

Download Full-text