A Reliable Liquid-Based CMOS MEMS Micro Thermal Convective Accelerometer With Enhanced Sensitivity and Limit of Detection

2021 ◽  
pp. 1-7
Author(s):  
Xiaoyi Wang ◽  
Yi-Kuen Lee ◽  
Wei Xu
Keyword(s):  
Limit Of Detection ◽  
Enhanced Sensitivity ◽  
Cmos Mems ◽  
Convective Accelerometer

scholarly journals Portable Real-Time Detection of Pb(II) Using a CMOS MEMS-Based Nanomechanical Sensing Array Modified with PEDOT:PSS

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2454
Author(s):  
Yi-Kuang Yen ◽  
Chao-Yu Lai
Keyword(s):  
Limit Of Detection ◽  
Quality Measurement ◽  
Complementary Metal Oxide Semiconductor ◽  
Cost Effective ◽  
Sample Volume ◽  
Small Sample ◽  
Oxide Semiconductor ◽  
Resistance Change ◽  
Site Water ◽  
Cmos Mems

Detecting the concentration of Pb2+ ions is important for monitoring the quality of water due to it can become a health threat as being in certain level. In this study, we report a nanomechanical Pb2+ sensor by employing the complementary metal-oxide-semiconductor microelectromechanical system (CMOS MEMS)-based piezoresistive microcantilevers coated with PEDOT:PSS sensing layers. Upon reaction with Pb2+, the PEDOT:PSS layer was oxidized which induced the surface stress change resulted in a subsequent bending of the microcantilever with the signal response of relative resistance change. This sensing platform has the advantages of being mass-produced, miniaturized, and portable. The sensor exhibited its sensitivity to Pb2+ concentrations in a linear range of 0.01–1000 ppm, and the limit of detection was 5 ppb. Moreover, the sensor showed the specificity to Pb2+, required a small sample volume and was easy to operate. Therefore, the proposed analytical method described here may be a sensitive, cost-effective and portable sensing tool for on-site water quality measurement and pollution detection.

CMOS MEMS Thermal Flow Sensor with Enhanced Sensitivity for HVAC Application

2020 ◽  
pp. 1-1 ◽  
Author(s):  
Wei Xu ◽  
Xiaoyi Wang ◽  
Ruijie Wang ◽  
Izhar ◽  
Jingcui Xu ◽  
...  
Keyword(s):  
Flow Sensor ◽  
Thermal Flow ◽  
Enhanced Sensitivity ◽  
Thermal Flow Sensor ◽  
Cmos Mems

From 2D to 3D FEM simulations of a CMOS MEMS convective accelerometer

2011 ◽  
Author(s):  
B. Mezghani ◽  
A. Brahim ◽  
F. Tounsi ◽  
M. Masmoudi ◽  
A. A. Rekik ◽  
...  
Keyword(s):  
3D Fem ◽  
Fem Simulations ◽  
Cmos Mems ◽  
Convective Accelerometer ◽  
2D To 3D

scholarly journals Development of an Enhanced Sensitivity Bead-Based Immunoassay for Real-Time In Vivo Detection of Pancreatic β-Cell Death

Endocrinology ◽  
2015 ◽  
Vol 156 (12) ◽  
pp. 4755-4760 ◽  
Author(s):  
Olivier R. Costa ◽  
Geert Stangé ◽  
Katrijn Verhaeghen ◽  
Benedicte Brackeva ◽  
Ellen Nonneman ◽  
...  
Keyword(s):  
Real Time ◽  
Limit Of Detection ◽  
Method Comparison ◽  
Analytical Sensitivity ◽  
Β Cells ◽  
Β Cell ◽  
Becton Dickinson ◽  
Time Resolved ◽  
Enhanced Sensitivity

There is a clinical need for plasma tests to detect and quantify the in vivo destruction of pancreatic β-cells in type 1 diabetes. We previously developed a time-resolved fluorescence immunoassay (TRFIA) to glutamate decarboxylase 65 kDa (GAD65) (GAD65-TRFIA) that was able to detect the synchronous necrotic destruction of transplanted β-cells in the hours after their infusion in the liver. This GAD65-TRFIA, however, lacked sensitivity to detect continued β-cell rejection beyond this acute phase. The aim of present study was to gain at least an order of magnitude in analytical sensitivity by switching to Becton Dickinson cytometric bead array (CBA) (GAD65-CBA) enhanced sensitivity format, using the same couple of monoclonal antibodies. We compared the performances of GAD65-CBA and GAD65-TRFIA using Clinical and Laboratory Standards Institute protocols for linearity, imprecision, specificity, limit of detection, and functional sensitivity. We conducted a method comparison and assessed the biologic potential on samples from human recipients of islet grafts. The GAD65-CBA showed acceptable linearity and imprecision. Switching from TRFIA to CBA lowered functional sensitivity by a factor 35 and lowered limit of detection by a factor 11 with minimal need for method optimization. The enhanced sensitivity greatly expands the application domain of our biomarker and allowed for the first time to detect ongoing β-cell destruction up to at least 1 day after islet transplantation. We conclude that the GAD65-CBA is suitable for biological and clinical assessment of the real-time destruction of β-cells in intraportal transplantation.

scholarly journals An SPR Sensor Chip Based on Peptide-Modified Single-Walled Carbon Nanotubes with Enhanced Sensitivity and Selectivity in the Detection of 2,4,6-Trinitrotoluene Explosives

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4461 ◽  
Author(s):  
Jin Wang ◽  
Sanyang Du ◽  
Takeshi Onodera ◽  
Rui Yatabe ◽  
Masayoshi Tanaka ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Limit Of Detection ◽  
Aromatic Amino Acids ◽  
Single Walled Carbon Nanotubes ◽  
Sensor Chip ◽  
Chip Surface ◽  
Single Walled Carbon ◽  
Spr Sensor ◽  
Enhanced Sensitivity ◽  
Walled Carbon Nanotubes

In this study, we developed a surface plasmon resonance (SPR) sensor chip based on 2,4,6-trinitrotoluene (TNT) recognition peptide-modified single-walled carbon nanotubes (SWCNTs). The carboxylic acid-functionalized SWCNTs were immobilized on a 3-aminopropyltriethoxysilane (APTES)-modified SPR Au chip surface. Through π-stacking between the aromatic amino acids and SWCNTs, the TNT recognition peptide TNTHCDR3 was immobilized onto the surface of the SWCNTs. The peptide–SWCNTs-modified sensor surface was confirmed and evaluated by atomic force microscope (AFM) observation. The peptide–SWCNTs hybrid SPR sensor chip exhibited enhanced sensitivity with a limit of detection (LOD) of 772 ppb and highly selective detection compared with commercialized carboxymethylated dextran matrix sensor chips.

scholarly journals Eco-Friendly Fluorescent ELISA Based on Bifunctional Phage for Ultrasensitive Detection of Ochratoxin A in Corn

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2429
Author(s):  
Weipeng Tong ◽  
Hao Fang ◽  
Hanpeng Xiong ◽  
Daixian Wei ◽  
Yuankui Leng ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Limit Of Detection ◽  
High Reliability ◽  
Ultra Performance Liquid Chromatography ◽  
Enzyme Linked Immunosorbent Assay ◽  
Enhanced Sensitivity ◽  
Great Specificity ◽  
Screening Platform ◽  
Mimic Peptide ◽  
Low Sensitivity

Conventional enzyme-linked immunosorbent assay (ELISA) is commonly used for Ochratoxin A (OTA) screening, but it is limited by low sensitivity and harmful competing antigens of enzyme-OTA conjugates. Herein, a bifunctional M13 bacteriophage with OTA mimotopes fused on the p3 protein and biotin modified on major p8 proteins was introduced as an eco-friendly competing antigen and enzyme container for enhanced sensitivity. Mercaptopropionic acid-modified quantum dots (MPA-QDs), which are extremely sensitive to hydrogen peroxide, were chosen as fluorescent signal transducers that could manifest glucose oxidase-induced fluorescence quenching in the presence of glucose. On these bases, a highly sensitive and eco-friendly fluorescent immunoassay for OTA sensing was developed. Under optimized conditions, the proposed method demonstrates a good linear detection of OTA from 4.8 to 625 pg/mL and a limit of detection (LOD) of 5.39 pg/mL. The LOD is approximately 26-fold lower than that of a conventional horse radish peroxidase (HRP) based ELISA and six-fold lower than that of a GOx-OTA conjugate-based fluorescent ELISA. The proposed method also shows great specificity and accepted accuracy for analyzing OTA in real corn samples. The detection results are highly consistent with those obtained using the ultra-performance liquid chromatography-fluorescence detection method, indicating the high reliability of the proposed method for OTA detection. In conclusion, the proposed method is an excellent OTA screening platform over a conventional ELISA and can be easily extended for sensing other analytes by altering specific mimic peptide sequences in phages.

Antibody immobilization techniques in mass sensitive immunosensor: enhanced sensitivity through limited mass load

2020 ◽  
Vol 16 ◽  
Author(s):  
Kübra Kırali ◽  
Nura Brimo ◽  
Dilek Çökeliler Serdaroğlu
Keyword(s):  
Acoustic Wave ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Load Level ◽  
Bulk Acoustic Wave ◽  
Receptor Interaction ◽  
Frequency Change ◽  
Antibody Immobilization ◽  
Enhanced Sensitivity ◽  
Mass Load

Background: Biosensors are analytical devices that include a sample-delivery approach between a biological recognition element and a transducer required to convert the physicochemical change produced from the interaction of biological molecules-receptor interaction into signal. The immunosensor is a special type of biosensors that includes an antibody as a biorecognition element to detect analyte as antigens. In mass-sensitive sensors, antigen-antibody interactions can be specified by measuring the frequency change and most commonly knowns are surface acoustic wave, bulk acoustic wave, quartz crystal microbalance and microcantilevers. Methods: Different methods for antibody immobilization including functionalization of the transducer surface with specific groups have been reported for antibody immobilization. This stage affects the limit of detection and overall performance. In this review, perspectives on immobilization strategies of mass sensitive immunosensors according to transducer types will be presented. The choice of immobilization methods and their impact on performance in terms of capture molecule loading, orientation and signal improvement is will also be discussed. Results: One of the most critical point during configuration of the biorecognition layer is to improve the sensitivity. Therefore, we initially focused on comparisons of the antibody immobilization strategies in the biorecognition layer in terms of mass load level and high sensitivity. Conclusion: The lack of significant data on the mass accumulations up to the functionalization and antibody immobilization steps, which are the basis of immusensor production, has been identified. However, mass sensitive immunosensors have the potential to become more common and effective analytical devices for many application areas.

scholarly journals Evaluation of 16S rRNA Hypervariable Regions for Bioweapon Species Detection by Massively Parallel Sequencing

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Victor H. G. Dias ◽  
Priscila da S. F. C. Gomes ◽  
Allan C. Azevedo-Martins ◽  
Bianca C. A. Cabral ◽  
August E. Woerner ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Limit Of Detection ◽  
Massively Parallel Sequencing ◽  
Bacterial Species ◽  
Massively Parallel ◽  
Rrna Gene ◽  
Parallel Sequencing ◽  
Enhanced Sensitivity ◽  
Hypervariable Regions

Molecular detection and classification of the bacterial groups in a sample are relevant in several areas, including medical research and forensics. Sanger sequencing of the 16S rRNA gene is considered the gold standard for microbial phylogenetic analysis. However, the development of massively parallel sequencing (MPS) offers enhanced sensitivity and specificity for microbiological analyses. In addition, 16S rRNA target amplification followed by MPS facilitates the combined use of multiple markers/regions, better discrimination of sample background, and higher sample throughput. We designed a novel set of 16S rRNA gene primers for detection of bacterial species associated with clinical, bioweapon, and biohazards microorganisms via alignment of 364 sequences representing 19 bacterial species and strains relevant to medical and forensics applications. In silico results indicated that the hypervariable regions (V1V2), (V4V5), and (V6V7V8) support the resolution of a selected group of bacteria. Interspecies and intraspecies comparisons showed 74.23%–85.51% and 94.48%–99.98% sequencing variation among species and strains, respectively. Sequence reads from a simulated scenario of bacterial species mapped to each of the three hypervariable regions of the respective species with different affinities. The minimum limit of detection was achieved using two different MPS platforms. This protocol can be used to detect or monitor as low as 2,000 genome equivalents of bacterial species associated with clinical, bioweapon, and biohazard microorganisms and potentially can distinguish natural outbreaks of pathogenic microorganisms from those occurring by intentional release.

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