scholarly journals Analysis of neurite outgrowth on electrospun fiber scaffolds in the presence of blebbistatin and paclitaxel

2015 ◽  
Author(s):  
A.R. D'Amato ◽  
N.J. Schaub ◽  
C.D.L. Johnson ◽  
J. Cardenas ◽  
R.J. Gilbert
Keyword(s):  
Neurite Outgrowth ◽  
Electrospun Fiber ◽  
Fiber Scaffolds

scholarly journals Electrospun Fiber Scaffolds for Engineering Glial Cell Behavior to Promote Neural Regeneration

2020 ◽  
Vol 8 (1) ◽  
pp. 4
Author(s):  
Devan L. Puhl ◽  
Jessica L. Funnell ◽  
Derek W. Nelson ◽  
Manoj K. Gottipati ◽  
Ryan J. Gilbert
Keyword(s):  
Nervous System ◽  
Glial Cell ◽  
Cell Response ◽  
Electrospun Fiber ◽  
Neural Regeneration ◽  
Cell Phenotype ◽  
Comprehensive Overview ◽  
Fiber Alignment ◽  
Wide Range ◽  
Fiber Scaffolds

Electrospinning is a fabrication technique used to produce nano- or micro- diameter fibers to generate biocompatible, biodegradable scaffolds for tissue engineering applications. Electrospun fiber scaffolds are advantageous for neural regeneration because they mimic the structure of the nervous system extracellular matrix and provide contact guidance for regenerating axons. Glia are non-neuronal regulatory cells that maintain homeostasis in the healthy nervous system and regulate regeneration in the injured nervous system. Electrospun fiber scaffolds offer a wide range of characteristics, such as fiber alignment, diameter, surface nanotopography, and surface chemistry that can be engineered to achieve a desired glial cell response to injury. Further, electrospun fibers can be loaded with drugs, nucleic acids, or proteins to provide the local, sustained release of such therapeutics to alter glial cell phenotype to better support regeneration. This review provides the first comprehensive overview of how electrospun fiber alignment, diameter, surface nanotopography, surface functionalization, and therapeutic delivery affect Schwann cells in the peripheral nervous system and astrocytes, oligodendrocytes, and microglia in the central nervous system both in vitro and in vivo. The information presented can be used to design and optimize electrospun fiber scaffolds to target glial cell response to mitigate nervous system injury and improve regeneration.

scholarly journals Poly-ε-Caprolactone/Halloysite Nanotube Composites for Resorbable Scaffolds: Effect of Processing Technology on Homogeneity and Electrospinning

Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3772
Author(s):  
Muriel Józó ◽  
Róbert Várdai ◽  
Nóra Hegyesi ◽  
János Móczó ◽  
Béla Pukánszky
Keyword(s):  
Electrospun Fiber ◽  
Fiber Spinning ◽  
Film Casting ◽  
Melt Mixing ◽  
Indirect Methods ◽  
Nanotube Composites ◽  
Simple Compression ◽  
Fiber Scaffolds ◽  
Direct And Indirect Methods

Polycaprolactone (PCL)/halloysite composites were prepared to compare the effect of homogenization technology on the structure and properties of the composites. Halloysite content changed from 0 to 10 vol% in six steps and homogeneity was characterized by various direct and indirect methods. The results showed that the extent of aggregation depends on technology and on halloysite content; the size and number of aggregates increase with increasing halloysite content. Melt mixing results in more homogeneous composites than the simple compression of the component powders or homogenization in solution and film casting. Homogeneity and the extent of aggregation determines all properties, including functionality. The mechanical properties of the polymer deteriorate with increasing aggregation; even stiffness depends on homogeneity. Strength and deformability decreases drastically as the number and size of aggregates increase. Not only dispersed structure, but also the physical state and crystalline structure of the polymer influence homogeneity and properties. The presence of the filler affects the preparation of electrospun fiber scaffolds as well. A part of the filler is excluded from the fibers while another part forms aggregates that complicates fiber spinning and deteriorates properties. The results indicate that spinning is easier and the quality of the fibers is better if a material homogenized previously by melt mixing is used for the production of the fibers.

scholarly journals Exploring the effects of electrospun fiber surface nanotopography on neurite outgrowth and branching in neuron cultures

PLoS ONE ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. e0211731 ◽  
Author(s):  
Anthony R. D’Amato ◽  
Devan L. Puhl ◽  
Alexis M. Ziemba ◽  
Christopher D. L. Johnson ◽  
Janneke Doedee ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Electrospun Fiber ◽  
Fiber Surface

Biointerface control of electrospun fiber scaffolds for bone regeneration: Engineered protein link to mineralized surface

2014 ◽  
Vol 10 (6) ◽  
pp. 2750-2761 ◽  
Author(s):  
Jae Ho Lee ◽  
Jeong-Hui Park ◽  
Ahmed El-Fiqi ◽  
Joong-Hyun Kim ◽  
Ye-Rang Yun ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Electrospun Fiber ◽  
Fiber Scaffolds

scholarly journals Modular assembly of thick multifunctional cardiac patches

2017 ◽  
Vol 114 (8) ◽  
pp. 1898-1903 ◽  
Author(s):  
Sharon Fleischer ◽  
Assaf Shapira ◽  
Ron Feiner ◽  
Tal Dvir
Keyword(s):  
Cardiac Tissue ◽  
Electrospun Fiber ◽  
Signal Propagation ◽  
Electrical Signal ◽  
Modular Assembly ◽  
Cardiac Tissues ◽  
Structure Morphology ◽  
Biomaterial Scaffolds ◽  
Fiber Scaffolds ◽  
And Function

In cardiac tissue engineering cells are seeded within porous biomaterial scaffolds to create functional cardiac patches. Here, we report on a bottom-up approach to assemble a modular tissue consisting of multiple layers with distinct structures and functions. Albumin electrospun fiber scaffolds were laser-patterned to create microgrooves for engineering aligned cardiac tissues exhibiting anisotropic electrical signal propagation. Microchannels were patterned within the scaffolds and seeded with endothelial cells to form closed lumens. Moreover, cage-like structures were patterned within the scaffolds and accommodated poly(lactic-co-glycolic acid) (PLGA) microparticulate systems that controlled the release of VEGF, which promotes vascularization, or dexamethasone, an anti-inflammatory agent. The structure, morphology, and function of each layer were characterized, and the tissue layers were grown separately in their optimal conditions. Before transplantation the tissue and microparticulate layers were integrated by an ECM-based biological glue to form thick 3D cardiac patches. Finally, the patches were transplanted in rats, and their vascularization was assessed. Because of the simple modularity of this approach, we believe that it could be used in the future to assemble other multicellular, thick, 3D, functional tissues.

scholarly journals Mueller Matrix Measurement of Electrospun Fiber Scaffolds for Tissue Engineering

Polymers ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2062 ◽  
Author(s):  
Dierk Fricke ◽  
Alexander Becker ◽  
Lennart Jütte ◽  
Michael Bode ◽  
Dominik de Cassan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Fiber Orientation ◽  
Electrospun Fiber ◽  
Mueller Matrix ◽  
Relative Orientation ◽  
Sem Images ◽  
Production Parameters ◽  
Fiber Scaffolds ◽  
Non Destructive

Electrospun fiber scaffolds are gaining in importance in the area of tissue engineering. They can be used, for example, to fabricate graded implants to mimic the tendon bone junction. For the grading of the tensile strength of the fiber scaffolds, the orientation of the fibers plays a major role. This is currently measured by hand in scanning electron microscope (SEM) images. In this work, a correlation between polarimetric information generated by measuring the Mueller matrix (MM) and the orientation of the fibers of electrospun fiber scaffolds is reported. For this, the MM of fiber scaffolds, which were manufactured with different production parameters, was measured and analyzed. These data were correlated with fiber orientation and mechanical properties, which were evaluated in an established manner. We found that by measurement of the MM the production parameters as well as the relative orientation of the fibers in space can be determined. Thus, the MM measurement is suitable as an alternative tool for non-contact, non-destructive determination of the production parameters and, thus, the degree of alignment of electrospun fiber scaffolds.

scholarly journals Solvent Retention in Electrospun Fibers Affects Scaffold Mechanical Properties

2018 ◽  
Vol 2 (1) ◽  
pp. 15-28 ◽  
Author(s):  
Anthony R. D’Amato ◽  
Michael T. K. Bramson ◽  
David T. Corr ◽  
Devan L. Puhl ◽  
Ryan J. Gilbert ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Electrospun Fiber ◽  
Ambient Air ◽  
Yield Strain ◽  
Scanning Calorimetry ◽  
Young’S Moduli ◽  
Solvent Retention ◽  
Fine Control ◽  
Fiber Scaffolds ◽  
The Impact

Abstract Electrospinning is a robust material fabrication method allowing for fine control of mechanical, chemical, and functional properties in scaffold manufacturing. Electrospun fiber scaffolds have gained prominence for their potential in a variety of applications such as tissue engineering and textile manufacturing, yet none have assessed the impact of solvent retention in fibers on the scaffold’s mechanical properties. In this study, we hypothesized that retained electrospinning solvent acts as a plasticizer, and gradual solvent evaporation, by storing fibers in ambient air, will cause significant increases in electrospun fiber scaffold brittleness and stiffness, and a significant decrease in scaffold toughness. Thermogravimetric analysis indicated solvent retention in PGA, PLCL, and PET fibers, and not in PU and PCL fibers. Differential scanning calorimetry revealed that polymers that were electrospun below their glass transition temperature (Tg) retained solvent and polymers electrospun above Tg did not. Young’s moduli increased and yield strain decreased for solventretaining PGA, PLCL, and PET fiber scaffolds as solvent evaporated from the scaffolds over a period of 14 days. Toughness and failure strain decreased for PGA and PET scaffolds as solvent evaporated. No significant differences were observed in the mechanical properties of PU and PCL scaffolds that did not retain solvent. These observations highlight the need to consider solvent retention following electrospinning and its potential effects on scaffold mechanical properties.

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