Silicon role on genipin-mediated crosslinking and in vitro apatite deposition over collagen hydrogels

2018 ◽  
Author(s):  
Dayana De Menezes ◽  
Gema Gonzalez ◽  
Karem Noris-Suarez
Keyword(s):  
Collagen Hydrogels ◽  
Apatite Deposition

Bioinspired mineralization of a functionalized injectable dense collagen hydrogel through silk sericin incorporation

2019 ◽  
Vol 7 (3) ◽  
pp. 1064-1077 ◽  
Author(s):  
Gabriele Griffanti ◽  
Wenge Jiang ◽  
Showan N. Nazhat
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Silk Sericin ◽  
Collagen Hydrogel ◽  
Biomineralization Process ◽  
Powerful Approach ◽  
Collagen Hydrogels ◽  
Stimulation Of

The incorporation of silk sericin into injectable dense collagen hydrogels represents a powerful approach to mimic the biomineralization process, together with the osteogenic stimulation of seeded mesenchymal stem cells, in vitro.

The negatively charged microenvironment of collagen hydrogels regulates the chondrogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo

2020 ◽  
Vol 8 (21) ◽  
pp. 4680-4693
Author(s):  
Jirong Yang ◽  
Yumei Xiao ◽  
Zizhao Tang ◽  
Zhaocong Luo ◽  
Dongxiao Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Protein Adsorption ◽  
Cell Morphology ◽  
Chondrogenic Differentiation ◽  
Collagen Hydrogels ◽  
Marrow Mesenchymal Stem Cells

The different negatively charged microenvironments of collagen hydrogels affect the protein adsorption, cell morphology, and chondrogenic differentiation of BMSCs in vitro and in vivo.

Apatite Deposition on Organic-Inorganic Hybrids Synthesized from Poly(Vinyl Alcohol) and Various Metal Oxides

2005 ◽  
Vol 284-286 ◽  
pp. 469-472 ◽  
Author(s):  
Caihong You ◽  
Toshiki Miyazaki ◽  
Eiichi Ishida ◽  
Masahiro Ashizuka ◽  
Chikara Ohtsuki ◽  
...  
Keyword(s):  
Metal Oxides ◽  
Vinyl Alcohol ◽  
Calcium Salt ◽  
Mechanical Performance ◽  
Bone Substitutes ◽  
Poly Vinyl Alcohol ◽  
Apatite Formation ◽  
Molybdenum Oxides ◽  
Apatite Deposition

Organic polymers with ability of apatite formation in body environment are expected as novel bone substitutes having not only bone-bonding ability, i.e. bioactivity, but also mechanical performance analogous to natural bone. Several metal oxides have been found to be effective for the apatite deposition in body environment. In addition, release of calcium ions from the materials significantly enhances it. In this study, we attempted to synthesize bioactive organic-inorganic hybrids from Poly(vinyl alcohol) (PVA) by incorporation of various metal oxides and calcium salt. Silica and molybdenum oxides were selected as metal oxides. Ability of apatite formation on the hybrids was examined in vitro using simulated body fluid (SBF, Kokubo solution). Apatite deposition were observed to occur on the surfaces of PVA/silica and PVA/molybdenum oxide hybrids in SBF, when their compositions were appropriately controlled.

Microstructure evolution in Stöber-type silica nanoparticles and their in vitro apatite deposition

2008 ◽  
Vol 48 (3) ◽  
pp. 322-335 ◽  
Author(s):  
Song Chen ◽  
Akiyoshi Osaka ◽  
Satoshi Hayakawa ◽  
Kanji Tsuru ◽  
Eiji Fujii ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Microstructure Evolution ◽  
Apatite Deposition

Bioactive Organic-Inorganic Hybrids Prepared from Poly(Vinyl Alcohol) via Modification with Metal Oxides and Calcium Salt

2006 ◽  
Vol 309-311 ◽  
pp. 1153-1156 ◽  
Author(s):  
Caihong You ◽  
Toshiki Miyazaki ◽  
Eiichi Ishida ◽  
Masahiro Ashizuka ◽  
Chikara Ohtsuki
Keyword(s):  
Metal Oxides ◽  
Titanium Oxide ◽  
Vinyl Alcohol ◽  
Calcium Salt ◽  
Mechanical Performance ◽  
Bone Substitutes ◽  
Poly Vinyl Alcohol ◽  
Apatite Formation ◽  
Apatite Deposition

Organic polymers with ability of apatite formation in body environment are expected as novel bone substitutes having not only bone-bonding ability, i.e. bioactivity, but also mechanical performance analogous to natural bone. Several metal oxides have been found to be effective for the apatite deposition in body environment. In addition, release of calcium ions from the materials significantly enhances it. In this study, we attempted to synthesize bioactive organic-inorganic hybrids from poly(vinyl alcohol) (PVA) by incorporation of titanium oxide or zirconium oxide as well as calcium salt. Ability of apatite formation on the hybrids was examined in vitro using simulated body fluid (SBF, Kokubo solution). Apatite deposition was observed to occur on the surfaces of PVA/titanium oxide hybrids in SBF, when their compositions were appropriately controlled.

scholarly journals Osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells encapsulated in Thai silk fibroin/collagen hydrogel: a pilot study in vitro

2019 ◽  
Vol 12 (6) ◽  
pp. 273-279 ◽  
Author(s):  
Jirun Apinun ◽  
Sittisak Honsawek ◽  
Somsak Kuptniratsaikul ◽  
Jutarat Jamkratoke ◽  
Sorada Kanokpanont
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Pilot Study ◽  
Osteogenic Differentiation ◽  
Collagen Hydrogel ◽  
Collagen Hydrogels ◽  
Rat Bone

AbstractBackgroundSilk fibroin (SF) can be processed into a hydrogel. SF/collagen hydrogel may be a suitable biomaterial for bone tissue engineering.ObjectivesTo investigate in vitro biocompatibility and osteogenic potential of encapsulated rat bone marrow-derived mesenchymal stem cells (rat MSCs) in an injectable Thai SF/collagen hydrogel induced by oleic acid–poloxamer 188 surfactant mixture in an in vitro pilot study.MethodsRat MSCs were encapsulated in 3 groups of hydrogel scaffolds (SF, SF with 0.05% collagen [SF/0.05C], and SF with 0.1% collagen [SF/0.1C]) and cultured in a growth medium and an osteogenic induction medium. DNA, alkaline phosphatase (ALP) activity, and calcium were assayed at periodically for up to 5 weeks. After 6 weeks of culture the cells were analyzed by scanning electron microscopy and energy dispersive spectroscopy.ResultsAlthough SF hydrogel with collagen seems to have less efficiency to encapsulate rat MSCs, their plateau phase growth in all hydrogels was comparable. Inability to maintain cell viability as cell populations declined over 1–5 days was observed. Cell numbers then plateaued and were maintained until day 14 of culture. ALP activity and calcium content of rat MSCs in SF/collagen hydrogels were highest at day 21. An enhancing effect of collagen combined with the hydrogel was observed for proliferation and matrix formation; however, benefits of the combination on osteogenic differentiation and biomineralization are as yet unclear.ConclusionRat MSCs in SF and SF/collagen hydrogels showed osteogenic differentiation. Accordingly, these hydrogels may serve as promising scaffolds for bone tissue engineering.

In Vitro Bioactivity of Hydrogen Peroxide Modified Titanium: Effects of Surface Morphology and Film Thickness

2006 ◽  
Vol 309-311 ◽  
pp. 407-410 ◽  
Author(s):  
Jin Ming Wu ◽  
Min Wang ◽  
Satoshi Hayakawa ◽  
Kanji Tsuru ◽  
Akiyoshi Osaka
Keyword(s):  
Hydrogen Peroxide ◽  
Surface Morphology ◽  
Film Thickness ◽  
Functional Groups ◽  
In Vitro Bioactivity ◽  
Apatite Deposition ◽  
Predominant Effect

Anatase films with various morphologies were formed on Ti surface through different approaches using hydrogen peroxide solutions. A porous anatase surface was found to favor apatite deposition in SBF, or in vitro bioactivity. Thicker films with wider crack gaps also showed enhanced in vitro bioactivity. However, the most predominant effect was the abundance of Ti-OH functional groups incorporated in the anatase films.

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