Concentrations of HSP27 and αβ‐crystallin in Oula Tibetan sheep meat and their relationship with meat quality during postmortem aging

Author(s):  
Xixiong Shi ◽  
Jie Li ◽  
Jingjing Chen ◽  
Zhu Tian ◽  
Cheng Chen ◽  
...  
2005 ◽  
Vol 2005 ◽  
pp. 45-45
Author(s):  
E. Karamichou ◽  
G.R. Nute ◽  
R.I. Richardson ◽  
K. McLean ◽  
S.C. Bishop

The development of genetic markers and their application to farm animals has progressed rapidly, opening new prospects for identifying chromosomal regions that control quantitative traits (quantitative trait loci or QTL). However, there is less activity in QTL identification in sheep than in other livestock species. Surprisingly few QTL have been published for traits of direct relevance to sheep meat production, apart from studies of individual major genes such as the callipyge locus (Freking et al, 2002). This suggests there may be more QTL effects still to be found in sheep. Hence, this study aims to identify QTL for carcass composition and meat quality traits. This will provide a basis for targeting genomic regions to verify QTL in independent sheep populations.


2014 ◽  
Vol 3 (3) ◽  
pp. 57 ◽  
Author(s):  
Samantha A. Hawkins ◽  
Brian Bowker ◽  
Hong Zhuang ◽  
Gary Gamble ◽  
Ronald Holser

<p>Chicken meat undergoes significant chemical and structural changes with postmortem time that influence meat quality characteristics. The objective of this study was to measure the visible-near infrared (vis-NIR) spectral differences in broiler breast fillets at 0.5, 4, 24, and 120 h postmortem. Muscle samples were flash frozen and freeze-dried prior to spectra analysis. In the visible region of the spectra (400-700 nm) changes in myoglobin protein peaks were observed with postmortem time. Freeze-drying muscle samples provided additional information from the NIR region of the spectra (800-2500 nm) on muscle protein changes during postmortem aging. Alterations to the b-sheet and a-helix structures of myofibrillar proteins and changes in the amount of bound water were observed in the NIR region with postmortem aging. Data from this study demonstrate that changes in breast fillets with postmortem time that are related to meat quality traits are detectable using vis-NIR spectroscopy.</p>


2021 ◽  
pp. 131611
Author(s):  
Xue Zhang ◽  
Lijuan Han ◽  
Shengzhen Hou ◽  
Sayed Haidar Abbas Raza ◽  
Zhiyou Wang ◽  
...  

2005 ◽  
Vol 45 (5) ◽  
pp. 593 ◽  
Author(s):  
O. A. Young ◽  
D. L. Hopkins ◽  
D. W. Pethick

The sheep meat eating quality research program has identified a number of outcomes and critical control points in the supply chain from live sheep genetics to cooked meat. The critical control points, which are largely independent of each other, can be translated into quality management systems to increase average eating quality of all cuts and lower variability. The choice of sire was a critical control point in that selecting for high growth rate and muscling can adversely affect eating quality. The challenge is to make sure that high yield traits are not promoted at the expense of eating quality. Animal age was a critical control point but it was clear that the definition of hogget could be revised to include slightly older animals with teeth in eruption but not in wear. Moreover, M. longissumus dorsi from older animals had only slightly lower eating quality than that from lamb such that this cut could be positioned as a premium product at all maturities, complementing the universally tender muscle psoas major. There was no doubt, however, that over all muscles, lamb remained the premium product. The critical control point for nutrition is that it be adequate, typically to ensure growth of at least 50 g/animal.day. Meat quality is improved through higher glycogen concentrations in muscles at slaughter, higher intramuscular fat content, and possibly reduced collagen crosslinking. Critical control points between muster and slaughter are more difficult to define but are generally aimed at stress reduction to minimise occurrence of the high ultimate pH condition. They include avoiding temperature extremes, loud noises and use of dogs, implementation of good lairage design and the use of skilled animal handlers. Stress is best monitored by ultimate pH measurements in abattoirs, rather than at remote points down the supply chain. After slaughter, the use of electrical stimulation to accelerate post mortem glycolysis is a critical control point. Its use is indicated where 2 conditions are simultaneously met: carcasses are Achilles-hung throughout processing, and the meat is destined for early consumption, as would normally be the case for the domestic market. By accelerating glycolysis, the temperature at rigor can be optimised for rapid tenderisation of low-connective tissue muscles through ageing. The alternative to electrical stimulation of these muscles for the local market is Tenderstretch hanging where rapid ageing is less temperature dependent. Where electrical stimulation is applied the monitoring of its effects with a temperature probe and a pH metre is a critical control point. Even where stimulation is not applied, measurement of average muscle temperature and pH is useful for defining any process. This is because the first 24 h after slaughter sets the scene for later meat storage/distribution, which has its own critical control point: the temperature at which meat is held between abattoir and consumption. Where meat is destined for early sale, the temperature of processing and storage can and should be higher than where the meat is destined for long-term storage as in export markets. For early sale, rapid ageing to optimum eating quality is promoted by higher temperature (2–4°C), whereas for export sale, very cool meat will slowly age in the weeks before consumption while at the same time minimising spoilage and maximising display life. Thus, matching the time–temperature profile of processing and storage to a particular market is a critical control point. The retail end of the supply chain has its own control points, principally display temperature and choice of display packaging, which have major effects on chilled display life. The cooler the better. Compared with conventional overwrap packs, modified atmosphere packs extend display life by typically 80%. However, these more sophisticated packs cost more and will not suit all domestic retailers. The matching of muscles by age with recommended cooking method is a critical control point at retail for ensuring consumer satisfaction. The challenge is effective communication with consumers and is part of the wider challenge of effectively communicating quality-related information at all links of the supply chain.


2007 ◽  
Vol 47 (10) ◽  
pp. 1229 ◽  
Author(s):  
R. D. Warner ◽  
D. W. Pethick ◽  
P. L. Greenwood ◽  
E. N. Ponnampalam ◽  
R. G. Banks ◽  
...  

The combined effects of age and genetics and Poll Dorset sire and growth path were studied in two separate experiments (n = 595 and 627, respectively). In the first experiment, containing genotype crosses typically used in Australia (Merino, Poll Dorset, Border Leicester) and sires selected for growth or muscling, sheep were slaughtered at 4, 8, 14 and 22 months. The second experiment used Poll Dorset sires selected for high muscling, fat or growth with progeny having two levels of nutrition postweaning. Border Leicesters expressed higher levels of carcass fat percentage and intramuscular fat and produced the heaviest carcass. Merinos had the lowest subcutaneous fat depth and highest carcass lean percentage when compared at the same age. The progeny of Poll Dorset sires selected for high muscling (PDm) expressed a shift toward glycolytic fibres relative to those from Merino sires, and PDm sires produced progeny with reduced spine and limb length and higher carcass muscle : mineral ratios, suggesting skeletal stunting. Genotype meat quality differences were minimal except that PDm sire topsides were tougher and Merinos produced higher pH meat. With age (4–22 months), lambs became heavier and fatter, fibres shifted towards oxidative and away from glycolytic, muscle myoglobin increased, the meat became darker and redder and tenderness declined. Early weaning had no effect on the time to reach slaughter weight, provided nutrition was not restricted. The sire genetics influence on the carcass composition far outweighed the effect of nutrition postweaning. Lambs on a restricted diet tended to have less acceptable meat quality but this was not evident in lambs from sires selected for high fatness. Sensory tenderness was improved and intramuscular fat was higher in lamb progeny from sires selected for high fatness.


2021 ◽  
Author(s):  
Jacob R. Tuell ◽  
Yu Qianqian ◽  
Brad Kim

Tumbling of intact muscle foods has been widely applied toprocessed meats using brine solution. However, the use of tumbling withoutbrine on fresh beef muscles has not been fully examined. Therefore, this studyaimed to evaluate fresh beef tumbling on meat quality and proteolytic featuresof loin (longissimus lumborum)muscles. Moreover, interactions with the duration of postmortem aging wereinvestigated. Loins (n=9) at 7d postmortem were sectioned and allocated among twotumbling (T) treatment groups at 60 (T60) or 90 (T90) minutes, as well as a non-tumbledcontrol (T0) group. After treatment, sub-sections were made and divided among0d, 7d, or 14d of further aging. Meat quality was assessed by shear forcevalues, water-holding ability, and color attributes. The extent of proteolysiswas determined by quantification of desmin and troponin-T, myofibrilfragmentation index (MFI), and transmission electron microscopy. An interactionbetween fresh beef tumbling and aging duration was observed in shear forcevalues (P=0.032). At 0d, muscles fromT90 exhibited lower shear force (21.6 N) compared to T0 (34.8 N) and T60 (24.7N) groups. Muscles from T60 and T90 groups maintained lower shear force than T0controls at each respective aging duration.Higher cooking loss (P=0.011) but notpurge loss (P=0.412) was observed in theT60 and T90 groups compared to T0. Shear force results were supported by higherMFI in T60 and T90 groups than T0 controls (P&lt;0.001), as well as the disappearance of intact troponin-T withfurther aging (P=0.009). Transmissionelectron microscopy supported increased initial tenderness would owe primarily tophysical disruptions to myofibrillar structure, though fresh beef tumbling may facilitateproteolysis with further aging.


PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11295
Author(s):  
Xuejiao An ◽  
Shengwei Zhang ◽  
Taotao Li ◽  
Nana Chen ◽  
Xia Wang ◽  
...  

To date, utilization of feed grains is increasing, which competes for human food. It is imperative to develop and utilize unconventional feed materials. Broussonetia papyrifera L. (B. papyrifera) is a good feeding material with high crude protein, crude fat, and low crude fiber, which is widely distributed in China. In this study, 12 Dorper ♂×Hu ♀  crossbred weaned male lambs were seleted into four groups based on the feed that ratio of the B. papyrifera fermented feed in the total mixed diet (0%, 6%, 18%, and 100%), to character the lambs’ longissimus dorsi (LD) fatty acids, morphology and transcriptome. Results showed that the muscle fiber’s diameter and area were the smallest in the 100% group. The highest content of beneficial fatty acids and the lowest content of harmful fatty acids in group 18%. RNA-seq identified 443 differentially expressed genes (DEGs) in the LD of lambs from 4 groups. Among these genes, 169 (38.1%) were up-regulated and 274 (61.9%) were down-regulated. The DEGs were mostly enriched in in fatty acid metabolism, arginine and proline metabolism, and PPAR signaling pathways. Our results provide knowledge to understand effect of different ratios of B. papyrifera fermented feed on sheep meat quality traits, also a basis for understanding of the molecular regulation mechanism of B. papyrifera fermented feed affecting on sheep meat quality.


2007 ◽  
Vol 47 (10) ◽  
pp. 1155 ◽  
Author(s):  
D. L. Hopkins ◽  
D. F. Stanley ◽  
L. C. Martin ◽  
E. S. Toohey ◽  
A. R. Gilmour

A 5 × 4 factorial experiment was designed in which lambs representing five genotypes were slaughtered at four ages (110, 236, 412 and 662 days of age or 4, 8, 14 and 22 months of age). The genotypes represented were Poll Dorsetgrowth × Border Leicester Merino (PDg × BLM), Poll Dorsetgrowth × Merino (PDg × M), Poll Dorsetmuscling × Merino (PDm × M), Merino × Merino (M × M) and Border Leicester × Merino (BL × M). In total, 595 animals were slaughtered and carcass traits, composition and meat quality measured. As the animals grew older, intramuscular fat percentage (IMF) increased in the longissimus (LL) muscle and as the proportion of Border Leicester parentage increased so did the levels of IMF. There was a significant sire breed effect on LL pH at 24 hours after slaughter (pH24), with Merino-sired animals having a higher pH24 than both Poll Dorset and BL-sired animals. A significant dam breed effect was found for pH24 of the semitendinosus (ST) muscle, with lambs born to Merino ewes having a higher pH than those born to BLM ewes. A significant effect of sire estimated breeding value (EBV) for yearling muscling (YEMD) on LL pH was detected indicating an increase in pH as the sire EBV for muscling increased. There was a significant Merino effect for ultimate pH of the ST, with Merino-sired animals having a higher ultimate pH than both Poll Dorset and Border Leicester-sired animals. Sucker lambs had a significantly lower ST pH than older animals. As animal age at slaughter increased, the LL became darker based on L* values. There was a significant increase in a* values as animals became older, with 14 and 22-month-old animals having redder muscle. As LL pH and YEMD EBV increased, a* values decreased. There was a significant interaction between animal age and post mortem aging time such that sucker lambs (4 months old) produced LL with the lowest shear force after 5 days of aging and exhibited the greatest decline in shear force with aging. There was a significant increase in semimembranosus muscle shear force for PDm-sired animals (mean ± s.e., 66.5 ± 1.99 Newtons) compared with other genotypes at 61.4 ± 1.28 Newtons, but this could not be attributed to YEMD.


2020 ◽  
Vol 104 (4) ◽  
pp. 1010-1023 ◽  
Author(s):  
Jianxin Jiao ◽  
Ting Wang ◽  
Jianwei Zhou ◽  
Abraham Allan Degen ◽  
Nana Gou ◽  
...  

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