Alpha‐lipoic acid may ameliorate testicular damage by targeting dox‐induced altered antioxidant parameters, mitofusin‐2 and apoptotic gene expression

Andrologia ◽  
2021 ◽  
Author(s):  
Elif Erdem Guzel ◽  
Nalan Kaya Tektemur ◽  
Ahmet Tektemur
Keyword(s):  
Gene Expression ◽  
Lipoic Acid ◽  
Alpha Lipoic Acid ◽  
Testicular Damage ◽  
Mitofusin 2 ◽  
Apoptotic Gene ◽  
Antioxidant Parameters

scholarly journals Effect of alpha-lipoic acid on antioxidant gene expression and kidney injury in alloxan-induced diabetic rats

2018 ◽  
Vol 8 (1) ◽  
pp. 6-6 ◽  
Author(s):  
Parisa Jamor ◽  
Hassan Ahmadvand ◽  
Hesam Ashoory ◽  
Esmaeel Babaeenezhad
Keyword(s):  
Gene Expression ◽  
Lipoic Acid ◽  
Kidney Injury ◽  
Diabetic Rats ◽  
Pcr Analysis ◽  
Control Group ◽  
Diabetic Patients ◽  
Alpha Lipoic Acid ◽  
The Impact ◽  
Gpx Activity

Background: Myeloperoxidase (MPO) is involved in the initiation, progression, and complications of atherosclerosis in diabetic patients. Objectives: In the current study, the impact of alpha-lipoic acid (LA), a natural antioxidant and a cofactor in the enzyme complexes on MPO, catalase (CAT) and glutathione peroxidase (GPx) activity, glutathione (GSH) and malondialdehyde (MDA) level, histopathology of kidney and expression of antioxidant enzymes, superoxide dismutase (SOD), GPx and CAT which are involved in the detoxification of reactive oxygen species (ROS), was evaluated in alloxan-induced diabetic rats. Materials and Methods: In this study, 30 male Rattus norvegicus rats randomly divided into three groups; control (C), non-treated diabetic (NTD), and LA-treated diabetics (LATD) was induced by alloxan monohydrate (100mg/kg; subcutaneous [SC]). Then treatment was performed with alphaLA (100 mg/kg intraperitoneal (i.p) daily to 6 weeks). Blood sample of animals collected to measure levels of MPO, CAT and GPx activity GSH and MDA. Kidney paraffin sections were prepared to estimate histological studies and to measure quantitative gene expression SOD, GPX and CAT in kidney. Results: Induction of diabetes led to a significant increase in MPO and MDA, reduced GSH level and GPx and CAT activities (P < 0.05). However, treatment with alpha-LA led to a significant elevation in GPx, CAT and GSH levels with a reduction in MPO activities and MDA levels (P < 0.05). Furthermore, the real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis results showed increased expressions of GPx, CAT and SOD enzyme in the treatment group compared with the diabetic control group. Histopathological lesions such as increased glomerular volume and lymphocyte infiltration were attenuated in the alpha-LA treated group. Conclusions: Our findings indicated that alpha-LA supplementation is effective in preventing complications induced by oxidative stress and atherosclerosis in diabetic rats.

Alpha-lipoic acid prevents arsenic toxicity in HepG2 cells by upregulation of GSH content, metabolic activity and HOX1 gene expression

2007 ◽  
Vol 172 ◽  
pp. S47-S48
Author(s):  
Selene Huerta-Olvera ◽  
José Macías-Barragán ◽  
Juan Armendáriz-Borunda ◽  
Fernando Diaz-Barriga ◽  
Fernando Siller-López
Keyword(s):  
Gene Expression ◽  
Metabolic Activity ◽  
Lipoic Acid ◽  
Hepg2 Cells ◽  
Arsenic Toxicity ◽  
Alpha Lipoic Acid

Alpha-lipoic acid regulates heme oxygenase gene expression and nuclear Nrf2 activation as a mechanism of protection against arsenic exposure in HepG2 cells

2010 ◽  
Vol 29 (2) ◽  
pp. 144-149 ◽  
Author(s):  
Selene G. Huerta-Olvera ◽  
José Macías-Barragán ◽  
Martha E. Ramos-Márquez ◽  
Juan Armendáriz-Borunda ◽  
Fernando Díaz-Barriga ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heme Oxygenase ◽  
Lipoic Acid ◽  
Hepg2 Cells ◽  
Arsenic Exposure ◽  
Alpha Lipoic Acid ◽  
Nrf2 Activation

scholarly journals α-Lipoic Acid Induces Intrinsic Apoptosis in Human Oral Squamous Carcinoma Cells

2021 ◽  
Author(s):  
Revathi Duraisamy ◽  
Ezhilarasan Devaraj ◽  
Elumalai Perumal
Keyword(s):  
Gene Expression ◽  
Lipoic Acid ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Oxygen Species ◽  
Intrinsic Apoptosis ◽  
Apoptotic Gene ◽  
Squamous Carcinoma Cells ◽  
Oral Squamous Carcinoma ◽  
Dual Staining

Abstract Oral squamous cell carcinoma is one of the leading cancers in India and it is responsible for significant morbidity and mortality. α -lipoic acid, a co-factor for several metabolic enzymes, suppresses the tumor growth. In this study, we investigated the α-lipoic acid-induced cytotoxicity and apoptosis in human oral squamous carcinoma (SCC-25) cells. α-lipoic acid treatments were given to SCC-25 cells for 24 h and cell proliferation was evaluated by MTT assay. The reactive oxygen species expression was examined by dichloro-dihydro-fluorescein diacetate assay. Apoptosis-related morphological changes were detected by dual staining. Cytochrome c and RAS (H-Ras) expression was measured by dual staining and RT-PCR respectively. Intrinsic apoptosis-related markers are analyzed using qPCR.α-lipoic acid inhibited SCC-25 cell proliferation in a concentration-dependent manner. This treatment also increased intracellular reactive oxygen species expression and the percentage of apoptotic cells (up to 70% of the cell population). Dual staining further confirms cytochrome c cytosolic expression. The oncogene H-Ras protein and gene expression was also down-regulated upon α-lipoic acid treatment in SCC-25 cells. qPCR analysis further confirms α-lipoic acid-induced an upregulation of bax, Apaf-1, caspase 3 and − 9, pro-apoptotic gene expressions and downregulation of bcl-2, an anti-apoptotic gene expression. The present results suggest that α-lipoic acid has cytotoxic and pro-apoptotic potential and it also downregulates H-Ras oncogene expression in human oral squamous carcinoma cells. α-lipoic acid may have promising role in the treatment of human oral squamous carcinoma.

scholarly journals Reduced alpha-lipoic acid synthase gene expression exacerbates atherosclerosis in diabetic apolipoprotein E-deficient mice

2012 ◽  
Vol 223 (1) ◽  
pp. 137-143 ◽  
Author(s):  
Xianwen Yi ◽  
Longquan Xu ◽  
Sylvia Hiller ◽  
Hyung-Suk Kim ◽  
Nobuyo Maeda
Keyword(s):  
Gene Expression ◽  
Apolipoprotein E ◽  
Lipoic Acid ◽  
Alpha Lipoic Acid ◽  
Deficient Mice

scholarly journals The effect of Alpha-lipoic acid in glyphosate treated granulosa cells on the human folliculogenesis genes

2021 ◽  
Author(s):  
Sakineh Kaboli Kafshgiri ◽  
Armin Attaranzadeh ◽  
Fahimeh Ghasemi ◽  
Ebrahim miri-moghaddam
Keyword(s):  
Gene Expression ◽  
Lipoic Acid ◽  
Granulosa Cells ◽  
Morphological Structure ◽  
Control Group ◽  
Alpha Lipoic Acid ◽  
Culture Cells ◽  
Wide Range ◽  
Male Patients ◽  
Star Gene

Abstract Pesticides have a wide range of infertility in female reproductive. GLP is the most common herbicide used worldwide. The present study tended to evaluate the effects of alpha-lipoic acid (ALA) on expression of folliculogenesis genes in human granulosa cells (GCs) treated with glyphosate. In this study, GC samples were taken from infertile male patients who had tubal factors and received ICSI or IVF treatment for the first time. The medium used to culture cells was DMEM-F12 containing FBS 10%, penicillin 1%, and streptomycin 1%. The cells were treated with glyphosate (GLP) (250 µg/ml), ALA (50 µg/ml), and their mixture (250 GLP + 50 ALA) for 24 hr. The FoxO1, NOX4, Vnn1, and STAR gene expression in GCs was determined by real-time PCR. Exposure to GLP decreased gene expression Vnn1 and STAR in the GCs, while in comparison to control group, FOXO1 and NOX4 expression insignificantly increased. Further, ALA treatment decreased FOXO1, NOX4, in the GCs, and increased VNN1 and STAR gene expression. Transcriptional expression of FoxO1, NOX4 and STAR in GCs also decreased in their combinations (GLY + ALA). At the same time, the level of Vnn1 mRNA in GCs was increased. The findings showed that exposure to GLP alters the morphological structure of GCs and the expression of follicogenic genes, leading to dysfunction of the reproductive system. Moreover, the results showed that cell characteristics were preserved efficiently in human GCs exposed to ALA as an antioxidant.

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