Growth performance, robustness against stress, serum insulin, IGF‐1 and GLUT4 gene expression of red tilapia ( Oreochromi s sp.) fed diet containing graded levels of creatine

Background: This study aimed to investigate the effects of guluronic acid (G2013) on blood sugar, insulin, and gene expression profile of oxLDL receptors (SR-A, CD36, LOX-1, and CD68) in the experimental model of diabetes. Methods: 18 Sprague Dawley rats were randomly assigned to three groups of healthy control, diabetic control, and G2013 group. Diabetes was induced through intraperitoneal (IP) injection of 60 mg/kg streptozotocin. The subjects were IP treated with 25 mg/kg of G2013 per day for 28 days. The body weight, food intake, fasting blood glucose and insulin were measured. In addition, the expression of mentioned genes was investigated through quantitative real-time PCR. Results: The data showed that the final weight increased significantly in the G2013-treated subjects compared to the diabetic control (p < 0.05). The results indicated that final food intake significantly reduced in the G2013-treated subjects compared to the diabetic control (p < 0.05). The study findings also suggested that the final fasting blood glucose significantly reduced in the G2013-treated group, whereas the final fasting serum insulin level significantly increased in this group compared to the diabetic control (p < 0.05). Moreover, the gene expression levels of SR-A, CD36, LOX-1, and CD68 in the G2013 group significantly reduced compared to the diabetic control (p < 0.05). Conclusion: This study showed that G2013, could reduce blood glucose and increase insulin levels and reduce the gene expression level of oxLDL receptors. In addition, it may probably play an important role in reducing the severity of diabetes-induced inflammatory symptoms.

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Growth performance, intestinal histomorphology, gut microflora and ghrelin gene expression analysis of broiler by supplementing natural growth promoters: A nutrigenomics approach

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2021.03.008 ◽

2021 ◽

Author(s):

Syed Muddassar Hussain Gilani ◽

Zubia Rashid ◽

Saddia Galani ◽

Sahar Ilyas ◽

Shagufta Sahar ◽

...

Keyword(s):

Gene Expression ◽

Growth Performance ◽

Expression Analysis ◽

Gene Expression Analysis ◽

Gut Microflora ◽

Natural Growth ◽

Growth Promoters ◽

Ghrelin Gene

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Effect of cereal type and plant extract addition on the growth performance, intestinal morphology, caecal microflora, and gut barriers gene expression of broiler chickens

animal ◽

10.1016/j.animal.2020.100056 ◽

2020 ◽

pp. 100056

Author(s):

M. Farahat ◽

D. Ibrahim ◽

A.T.Y. Kishawy ◽

H.M. Abdallah ◽

A. Hernandez-Santana ◽

...

Keyword(s):

Gene Expression ◽

Growth Performance ◽

Plant Extract ◽

Broiler Chickens ◽

Intestinal Morphology

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PSIV-6 Effect of a Phytogenic Water Additive on Growth Performance, Blood Metabolites and Gene Expression of Amino Acids Transporters in Nursery Pigs Fed with Low Protein Diets

Journal of Animal Science ◽

10.1093/jas/skaa278.507 ◽

2020 ◽

Vol 98 (Supplement_4) ◽

pp. 281-282

Author(s):

Cedrick N Shili ◽

Mohammad Habibi ◽

Julia Sutton ◽

Jessie Barnes ◽

Jacob Burchkonda ◽

...

Keyword(s):

Gene Expression ◽

Amino Acids ◽

Growth Performance ◽

Mrna Abundance ◽

Protein Diet ◽

Low Protein Diet ◽

Blood Calcium ◽

Low Protein ◽

Protein Diets ◽

Standard Protein

Abstract Moderately low protein (MLP) diets can help decrease nutrient excretion from the swine production. However, MLP diets negatively impact growth performance. We hypothesized that supplementing MLP diets with phytogenics may reduce the negative effects of these diets on growth. The objective of this study was to investigate the effect of a phytogenic water additive (PWA; Herbanimal®) on growth performance, blood metabolite and gene expression of amino acids transporters in pigs fed with MLP diets. Forty-eight weaned barrows were allotted to six dietary treatments (n = 8) for 4 weeks: >CON-NS: standard protein diet-no PWA; CON-LS: standard protein diet-low PWA dose (4 ml/L); CON-HS: standard protein diet-high PWA dose (8 ml/L); LP-NS: low protein diet-no PWA; LP-LS: low protein diet-low PWA dose (4 ml/L); LP-HS: low protein diet- high PWA dose (8 ml/L). Feed intake and body weight were recorded daily and weekly, respectively. At week 4, blood and tissue samples were collected and analyzed for metabolites using a chemistry analyzer and amino acid transporters using qPCR, respectively. The data were analyzed by univariate GLM (SPSS®) and the means were separated using paired Student’s t-test corrected by Benjamini-Hochberg. Pigs fed CON-HS improved the average daily gain and serum calcium and phosphorus concentrations compared to CON-NS. Pigs fed LP-LS had higher serum phosphorus and blood urea nitrogen compared to the pigs fed with LP-NS. The mRNA abundance of SLC7A11 in the jejunum was lower in CON-LS and CON-HS compared to CON-NS. Additionally, mRNA abundance of SLC6A19 in the jejunum of pigs fed with LP-LS was higher compared to LP-NS and lower in CON-HS relative to pigs fed with CON-LS. In conclusion, PWA improved the growth performance of pigs fed standard protein diets but not low protein diets. Further, the PWA improved the concentrations of blood calcium and phosphorous in pigs fed MLP diets. Funding: Agrivida and Animal Health and Production and Animal Products: Improved Nutritional Performance, Growth, and Lactation of Animals from the USDA-NIFA.

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