Adipose stem cell‐derived exosomes in combination with hyaluronic acid accelerate wound healing through enhancing re‐epithelialization and vascularization

Injectable silk nanofiber hdyrogels were used to load stem cells and regulate cell behaviors effectively. The stem cell–laden silk hydrogels accelerated wound healing with higher quality and hair follicle regeneration.

Download Full-text

Thiolated Carboxymethyl-Hyaluronic-Acid-Based Biomaterials Enhance Wound Healing in Rats, Dogs, and Horses

ISRN Veterinary Science ◽

10.5402/2011/851593 ◽

2011 ◽

Vol 2011 ◽

pp. 1-7 ◽

Cited By ~ 15

Author(s):

Guanghui Yang ◽

Glenn D. Prestwich ◽

Brenda K. Mann

Keyword(s):

Wound Healing ◽

Veterinary Medicine ◽

Hyaluronic Acid ◽

Wound Closure ◽

Keratinocyte Proliferation ◽

Accelerate Wound Healing ◽

Multiple Species ◽

Enhance Wound Healing

The progression of wound healing is a complicated but well-known process involving many factors, yet there are few products on the market that enhance and accelerate wound healing. This is particularly problematic in veterinary medicine where multiple species must be treated and large animals heal slower, oftentimes with complicating factors such as the development of exuberant granulation tissue. In this study a crosslinked-hyaluronic-acid (HA-) based biomaterial was used to treat wounds on multiple species: rats, dogs, and horses. The base molecule, thiolated carboxymethyl HA, was first found to increase keratinocyte proliferation in vitro. Crosslinked gels and films were then both found to enhance the rate of wound healing in rats and resulted in thicker epidermis than untreated controls. Crosslinked films were used to treat wounds on forelimbs of dogs and horses. Although wounds healed slower compared to rats, the films again enhanced wound healing compared to untreated controls, both in terms of wound closure and quality of tissue. This study indicates that these crosslinked HA-based biomaterials enhance wound healing across multiple species and therefore may prove particularly useful in veterinary medicine. Reduced wound closure times and better quality of healed tissue would decrease risk of infection and pain associated with open wounds.

Download Full-text

Wound‐healing effect of adipose stem cell‐derived extracellular matrix sheet on full‐thickness skin defect rat model: Histological and immunohistochemical study

International Wound Journal ◽

10.1111/iwj.13030 ◽

2018 ◽

Vol 16 (1) ◽

pp. 286-296 ◽

Cited By ~ 4

Author(s):

Yoon J. Lee ◽

Seung E. Baek ◽

Sujin Lee ◽

Yeon J. Jeong ◽

Ki J. Kim ◽

...

Keyword(s):

Extracellular Matrix ◽

Wound Healing ◽

Stem Cell ◽

Rat Model ◽

Immunohistochemical Study ◽

Skin Defect ◽

Full Thickness ◽

Adipose Stem Cell ◽

Thickness Skin ◽

Wound Healing Effect

Download Full-text

Stem Cell-Containing Hyaluronic Acid-Based Spongy Hydrogels for Integrated Diabetic Wound Healing

Journal of Investigative Dermatology ◽

10.1016/j.jid.2017.02.976 ◽

2017 ◽

Vol 137 (7) ◽

pp. 1541-1551 ◽

Cited By ~ 15

Author(s):

Lucília Pereira da Silva ◽

Tírcia Carlos Santos ◽

Daniel Barreira Rodrigues ◽

Rogério Pedro Pirraco ◽

Mariana Teixeira Cerqueira ◽

...

Keyword(s):

Wound Healing ◽

Stem Cell ◽

Hyaluronic Acid ◽

Diabetic Wound ◽

Diabetic Wound Healing

Download Full-text

Combination of Two Kinds of Medicated Microparticles Based on Hyaluronic Acid or Chitosan for a Wound Healing Spray Patch

Pharmaceutics ◽

10.3390/pharmaceutics13122195 ◽

2021 ◽

Vol 13 (12) ◽

pp. 2195

Author(s):

Angela Fabiano ◽

Chiara Migone ◽

Luca Cerri ◽

Anna Maria Piras ◽

Andrea Mezzetta ◽

...

Keyword(s):

Wound Healing ◽

Hyaluronic Acid ◽

Second Life ◽

Olive Leaves ◽

Waste Products ◽

Accelerate Wound Healing ◽

Scratch Wound ◽

Treatment Of Wounds ◽

Scratch Wound Healing Assay

Olive leaves extract (OLE) has been extensively studied as antioxidant and antibiotic and these characteristics make it particularly interesting for use on wounds. For this reason, the aim of this study was to introduce OLE in microparticles (MP) of hyaluronic acid (MPHA-OLE) or chitosan (MPCs-OLE) to obtain a spray patch for the treatment of wounds in anatomical areas that are difficult to protect with traditional patches. The MP were characterized for particle size and ability to protect OLE from degradation, to absorb water from wound exudate, to control OLE release from MP. The MPHA and MPCs medicated or not and mixtures of the two types in different proportions were studied in vitro on fibroblasts by the scratch wound healing assay. The MP size was always less than 5 µm, and therefore, suitable for a spray patch. The MPCs-OLE could slow down the release of OLE therefore only about 60% of the polyphenols contained in it were released after 4 h. Both MPHA and MPCs could accelerate wound healing. A 50% MPHA-OLE-50% MPCs-OLE blend was the most suitable for accelerating wound healing. The MPHA-OLE-MPCs-OLE blends studied in this work were shown to have the characteristics suitable for a spray patch, thus giving a second life to the waste products of olive growers.

Download Full-text

The Effects of Cytokines in Adipose Stem Cell-Conditioned Medium on the Migration and Proliferation of Skin FibroblastsIn Vitro

BioMed Research International ◽

10.1155/2013/578479 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 24

Author(s):

Jiajia Zhao ◽

Li Hu ◽

Jiarong Liu ◽

Niya Gong ◽

Lili Chen

Keyword(s):

Wound Healing ◽

Stem Cell ◽

Conditioned Medium ◽

Healing Process ◽

Dermal Fibroblasts ◽

Wound Healing Process ◽

Adipose Stem Cell ◽

And Migration ◽

Cell Conditioned Medium ◽

Proliferation And Migration

Although adipose stem cell-conditioned medium (ASC-CM) has demonstrated the effect of promoting the cutaneous wound healing, the mechanism for this response on the effector cells (e.g., dermal fibroblasts) during the process remains to be determined. In this study, we aim to investigate the types and contents of cytokines in ASC-CM and the effects of some kinds of common cytokines in ASC-CM, such as EGF, PDGF-AA, VEGF, and bFGF, on dermal fibroblasts proliferation and migration in wound healing process. Results showed that these four cytokines had high concentrations in ASC-CM. The migration of skin fibroblasts could be significantly stimulated by VEGF, bFGF, and PDGF-AA, and the proliferation could be significantly stimulated by bFGF and EGF in ASC-CM. Additionally, ASC-CM had more obvious promoting effect on fibroblasts proliferation and migration than single cytokine. These observations suggested that ASC-CM played an important role in the cutaneous injury partly by the synergistic actions of several cytokines in promoting dermal fibroblasts proliferation and migration, and ASC-CM was more adaptive than each single cytokine to be applied in promoting the wound healing.

Download Full-text

Adipose-derived stem cell-containing hyaluronic acid/alginate hydrogel improves vocal fold wound healing

The Laryngoscope ◽

10.1002/lary.24405 ◽

2013 ◽

Vol 124 (3) ◽

pp. E64-E72 ◽

Cited By ~ 45

Author(s):

Young-Mo Kim ◽

Se H. Oh ◽

Jeong-Seok Choi ◽

Songyi Lee ◽

Jeong C. Ra ◽

...

Keyword(s):

Wound Healing ◽

Stem Cell ◽

Hyaluronic Acid ◽

Vocal Fold ◽

Alginate Hydrogel ◽

Adipose Derived Stem Cell

Download Full-text

Adipose stem cell secretion combined with biomaterials facilitates large-area wound healing

Regenerative Medicine ◽

10.2217/rme-2020-0086 ◽

2020 ◽

Author(s):

Nan Li ◽

Baoshuai Bai ◽

Hairong Zhang ◽

Wei Zhang ◽

Shengjian Tang

Keyword(s):

Wound Healing ◽

Stem Cell ◽

Tissue Loss ◽

Entire Body ◽

Cell Secretion ◽

Adipose Stem Cell ◽

Large Area ◽

Fast Pace ◽

Biomaterial Scaffolds ◽

Adipose Derived Stem Cell

Adipose-derived stem cell (ADSC)-based therapeutic strategies are in fast-pace advancement in wound treatment due to their availability and the ability to self-renew, undergo multilineage differentiation and self-renewal. Existing studies have successfully explored ADSCs to facilitate scar-free healing of small wounds, but whether the healing of large-area wounds that exhibit over 50% of skin tissue loss in the entire body could be achieved remains controversial. This study sought to review the mechanism of physiological wound healing, and discuss the roles played by chemokines, biological factors and biomaterial scaffolds. The possibility of applying ADSC-conditioned medium or ADSC-released exosomes as ‘off-the-shelf’ tissue engineering products, integrated with biomaterial scaffolds to facilitate wound healing, was analyzed.

Download Full-text