Effect of chitosan solutions with or without fluoride on the protection against dentin erosion in vitro

2020 ◽  
Vol 128 (6) ◽  
pp. 495-500
Author(s):  
Beatriz M Souza ◽  
Paula F Machado ◽  
Luiz RP Vecchia ◽  
Ana C Magalhães
Keyword(s):  
2021 ◽  
Author(s):  
Lethycia Almeida Santos ◽  
Tatiana Martini ◽  
João Victor Frazão Câmara ◽  
Fabiana Navas Reis ◽  
Adriana de Cássia Ortiz ◽  
...  

The effect of solutions and gels containing a sugarcane-derived cystatin (CaneCPI-5) on the protection against enamel and dentin erosion in vitro was evaluated. Bovine enamel and dentin specimens were divided into two groups (n=135 and 153/group for enamel and dentin, respectively) that were treated with solutions or chitosan gels containing 0.1 or 0.25 mg/ml CaneCPI-5. The positive controls for solutions and gels were Elmex Erosion Protection™ solution and NaF gel (12,300 ppm F), respectively. Deionized water and chitosan gel served as controls, respectively. The solutions were first applied on the specimens for 1 min and the gels for 4 min. Stimulated saliva was collected from 3 donors and used to form a 2 h acquired pellicle on the specimens. Then, the specimens were submitted to an erosive pH cycling protocol 4 times/day for 7 days (0.1% citric acid pH 2.5/90s, artificial saliva/2h, artificial saliva overnight). The solutions and gels were applied again during pH cycling, 2 times/day for 1 min and 4 min, respectively, after the first and last erosive challenges. Enamel and dentin losses (µm) were assessed by contact profilometry. Data were analyzed by 2-way ANOVA and Tukey´s test (p <0.05). All the treatments significantly reduced enamel and dentin loss in comparison with controls. Both CaneCPI-5 concentrations had a similar protective effect against enamel erosion, but only the higher concentration was as effective against dentin erosion as the positive control. Regarding the vehicles, only the 0.1 mg/ml gel performed worse than the positive control for dentin. CaneCPI-5 reduced enamel and dentin erosion to a similar extent as the fluoride-containing vehicles. However, dentin requires higher CaneCPI-5 concentrations, in the case of gels. Solutions or gels containing CaneCPI-5 might be a new approach to protect against dental erosion.


2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Lívia Picchi Comar ◽  
Marina Franciscon Gomes ◽  
Naiana Ito ◽  
Priscila Aranda Salomão ◽  
Larissa Tercília Grizzo ◽  
...  

The aim of this study was to compare the effect of toothpastes containing TiF4, NaF, and SnF2on tooth erosion-abrasion. Bovine enamel and dentin specimens were distributed into 10 groups (n=12): experimental placebo toothpaste (no F); NaF (1450 ppm F); TiF4(1450 ppm F); SnF2(1450 ppm F); SnF2(1100 ppm F) + NaF (350 ppm F); TiF4(1100 ppm F) + NaF (350 ppm F); commercial toothpaste Pro-Health (SnF2—1100 ppm F + NaF—350 ppm F, Oral B); commercial toothpaste Crest (NaF—1.500 ppm F, Procter & Gamble); abrasion without toothpaste and only erosion. The erosion was performed 4 × 90 s/day (Sprite Zero). The toothpastes’ slurries were applied and the specimens abraded using an electric toothbrush 2 × 15 s/day. Between the erosive and abrasive challenges, the specimens remained in artificial saliva. After 7 days, the tooth wear was evaluated using contact profilometry (μm). The experimental toothpastes with NaF, TiF4, SnF2, and Pro-Health showed a significant reduction in enamel wear (between 42% and 54%). Pro-Health also significantly reduced the dentin wear. The toothpastes with SnF2/NaF and TiF4/NaF showed the best results in the reduction of enamel wear (62–70%) as well as TiF4, SnF2, SnF2/NaF, and TiF4/NaF for dentin wear (64–79%) (P<0.05). Therefore, the experimental toothpastes containing both conventional and metal fluoride seem to be promising in reducing tooth wear.


2014 ◽  
Vol 2 (2) ◽  
pp. 159
Author(s):  
Ana Luiza Ubinha Moino ◽  
Catarina Ribeiro Barros Alencar ◽  
Maisa Camilo Jordão ◽  
Marília Afonso Rabelo Buzalaf ◽  
Ana Carolina Magalhães ◽  
...  

AIM: To compare the erosive effect of different light and sugar free beverages in relation to their regular version, on dentin. MATERIALS AND METHODS: Bovine root dentin samples were randomized into 9 different types of drinks (each n=12): Coca-cola®, Light Coca-cola®, Coca-cola® Zero, Guaraná Antartica®, Guaraná Antartica® Zero, Sprite®, Sprite® Zero, Del Valle® Passion fruit juice and Del Valle® Light Passion fruit juice. During each one of the 10 experimental days, the samples were subjected to 3 erosive demineralization (by immersion of the sample in the studied drink) and remineralization (by immersion of the sample in artificial saliva) cycles. Profilometry was evaluated at the end of the 1st, 5th and 10th days. For each type of drink, the data were tested using 2 way ANOVA and Bonferroni test (p<0.05). RESULTS: Erosive cycling with regular cola resulted in a higher wear on the first day, on the 5th day the regular drink was similar to zero and greater than light version, on the 10th day there was no difference between the versions of cola drink. For the Sprite drink there was difference between the versions just in the 10th day, in which the higher wear was promoted by the zero version. For guaraná and juice beverages there was no differences between versions in relation to the resulted erosive wear. CONCLUSION: Erosive tooth wear promoted by light and sugar-free or regular versions of soft drinks and juices showed distinct behaviours over the cycling time.


2013 ◽  
Vol 21 (6) ◽  
pp. 597-600 ◽  
Author(s):  
Andre de Carvalho SALES-PERES ◽  
Juliane Avansini MARSICANO ◽  
Rudan Paraiso GARCIA ◽  
Moacir Rossi FORIM ◽  
Maria Fatima das Gracas Fernandes da SILVA ◽  
...  
Keyword(s):  

2015 ◽  
Vol 29 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Amnah Abdullah ALGARNI ◽  
Frank LIPPERT ◽  
Anderson Takeo HARA

2010 ◽  
Vol 38 (2) ◽  
pp. 153-157 ◽  
Author(s):  
Ana Carolina Magalhães ◽  
Flávia Mauad Levy ◽  
Daniela Rios ◽  
Marília Afonso Rabelo Buzalaf

2015 ◽  
Vol 26 (3) ◽  
pp. 263-267
Author(s):  
Stella S. Ferreira ◽  
Tais Scaramucci ◽  
Anderson T. Hara ◽  
Idalina V. Aoki ◽  
Maria Angela Pita Sobral

Protein supplementation may be an alternative to reduce the erosive potential of acidic drinks. The aim of this in vitro study was to evaluate the erosive potential of an orange juice modified by dietary proteins. A commercially available orange juice was added 0.2 g/L casein, 2.0 g/L ovalbumin and their combination. The juice with no additives and a commercially available calcium-modified juice were used as negative and positive controls, respectively. Human enamel and dentin specimens (n=11) were tested in an erosion-remineralization cycling model. Enamel was analyzed by surface microhardness and profilometry, whilst dentin by profilometry only. Statistical analyses were performed using one-way ANOVA followed by Tukey's test (p<0.05). Calcium-modified juice showed the lowest erosive potential for both analyses (p<0.05). For enamel, the protein-added groups did not differ from each other (p>0.05) and showed significantly lower enamel loss compared to negative control (p<0.05). Regarding surface microhardness, casein showed the highest values compared to negative control (p<0.05). For dentin, none of the protein-added groups showed lower values of surface loss compared to negative control (p>0.05). In conclusion, for enamel the protein-modified orange juices presented reduced erosion of enamel, with casein showing a trend for better protection. For dentin, no reduction in the erosive potential was observed for the tested protein-modified orange juices.


2020 ◽  
Vol 54 (3) ◽  
pp. 292-296
Author(s):  
Marcelle Danelon ◽  
Juliano Pelim Pessan ◽  
Kamila Miranda Prado ◽  
Jéssica Paula Ramos ◽  
Nayara Gonçalves Emerenciano ◽  
...  

This in vitro study evaluated the protective effect of fluoride varnishes containing sodium trimetaphosphate (TMP) against dentin erosion and abrasion. Specimens of coronal dentin were divided into: placebo, 2.5% NaF, 5% NaF, 2.5% NaF + 5% TMP, and 5% NaF + 5% TMP groups (n =24/group). After single application of the varnishes, the samples were immersed in citric acid (0.05 mol/L, pH = 3.2, 5 min) followed or not by brushing, and the dentin wear was assessed after 5 days. Varnishes containing fluoride + TMP led to the lowest wear. TMP varnishes showed a superior effect against dentin erosive wear.


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