Innate lymphoid cells in peripheral blood of patients with American Cutaneous Leishmaniasis

2021 ◽  
Author(s):  
Orquídea L. Rodríguez ◽  
Dennis A. Lugo ◽  
Maira Cabrera ◽  
Martín A. Sánchez ◽  
Olga Zerpa ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Peripheral Blood ◽  
Innate Lymphoid Cells ◽  
Lymphoid Cells ◽  
American Cutaneous Leishmaniasis

scholarly journals Microbiota instruct IL-17A-producing innate lymphoid cells to promote skin inflammation in cutaneous leishmaniasis

2021 ◽  
Author(s):  
Tej Pratap Singh ◽  
Augusto Carvalho ◽  
Elizabeth Grice ◽  
Phillip Scott
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Staphylococcus Epidermidis ◽  
Leishmania Major ◽  
Inflammatory Responses ◽  
Skin Inflammation ◽  
Innate Lymphoid Cells ◽  
Lymphoid Cells ◽  
Skin Microbiota ◽  
Infected Skin

p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica} span.s1 {color: #222222} span.s2 {font: 8.0px Helvetica} Innate lymphoid cells (ILCs) comprise a heterogeneous population of immune cells that maintain barrier function and can initiate a protective or pathological immune response upon infection. Here we show the involvement of IL-17A-producing ILCs in microbiota-driven immunopathology in cutaneous leishmaniasis. IL-17A-producing ILCs were RORgt + and were enriched in Leishmania major infected skin, and topical colonization with Staphylococcus epidermidis before L. major infection exacerbated the skin inflammatory responses and IL-17A-producing RORgt + ILC accumulation without impacting type 1 immune responses. IL-17A responses in ILCs were directed by Batf3 dependent CD103 + dendritic cells, and experiments using ILC deficient Rag1 -/- mice established that IL-17A + ILCs were sufficient in driving the inflammatory responses. As depletion of ILCs or neutralization of IL-17A diminished the microbiota mediated immunopathology. Taken together, this study indicates that the skin microbiota promotes RORgt + IL-17A-producing ILCs, which augment the skin inflammation in cutaneous leishmaniasis.

scholarly journals OMIP‐055: Characterization of Human Innate Lymphoid Cells from Neonatal and Peripheral Blood

2019 ◽  
Vol 95 (4) ◽  
pp. 427-430 ◽  
Author(s):  
Sabrina Bianca Bennstein ◽  
Angela Riccarda Manser ◽  
Sandra Weinhold ◽  
Nadine Scherenschlich ◽  
Markus Uhrberg
Keyword(s):  
Peripheral Blood ◽  
Innate Lymphoid Cells ◽  
Lymphoid Cells

scholarly journals Targeted deletion of NFAT-Interacting-Protein-(NIP) 45 resolves experimental asthma by inhibiting Innate Lymphoid Cells group 2 (ILC2)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sonja Koch ◽  
Lisa Knipfer ◽  
Julia Kölle ◽  
Hooman Mirzakhani ◽  
Anna Graser ◽  
...  
Keyword(s):  
T Cells ◽  
Transcription Factor ◽  
Peripheral Blood ◽  
Innate Lymphoid Cells ◽  
Lymphoid Cells ◽  
Mucus Production ◽  
Interacting Protein ◽  
Targeted Deletion ◽  
Airway Pathology ◽  
Group 2

Abstract Here we investigated the role of NFAT-interacting protein (NIP)-45, an Interleukin (IL)-4 inducing Transcription Factor, and its impact on the differentiation of Group 2 Innate -Lymphoid -Cells (ILC2s) in the pathogenesis of asthma. NIP45, a transcription factor regulating NFATc1 activity, mRNA was found to be induced in the Peripheral Blood mononuclear cells (PMBCs) of asthmatic pre-school children with allergies and in the peripheral blood CD4+ T cells from adult asthmatic patients. In PBMCs of asthmatic and control children, NIP45 mRNA directly correlated with NFATc1 but not with T-bet. Targeted deletion of NIP45 in mice resulted in a protective phenotype in experimental asthma with reduced airway mucus production, airway hyperresponsiveness and eosinophils. This phenotype was reversed by intranasal delivery of recombinant r-IL-33. Consistently, ILC2s and not GATA3+ CD4+ T-cells were decreased in the lungs of asthmatic NIP45−/− mice. Reduced cell number spleen ILC2s could be differentiated from NIP45−/− as compared to wild-type mice after in vivo injection of a microcircle-DNA vector expressing IL-25 and decreased cytokines and ILC2 markers in ILC2 differentiated from the bone marrow of NIP45−/− mice. NIP45 thus emerges as a new therapeutic target for the resolution of the airway pathology, down-regulation of ILC2s and mucus production in asthma.

scholarly journals Circulating Innate Lymphoid Cells Exhibit Distinctive Distribution During Normal Pregnancy

2022 ◽  
Author(s):  
Yiran Zhao ◽  
Yajie Zhu ◽  
Xi Chen ◽  
Hui Lin ◽  
Ningxin Qin ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Peripheral Blood ◽  
Progesterone Receptors ◽  
Normal Pregnancy ◽  
Innate Lymphoid Cells ◽  
Sex Hormone ◽  
Lymphoid Cells ◽  
Hormone Levels ◽  
Potential Association ◽  
Plasma Estradiol

AbstractOver the past decades, the investigation of innate lymphoid cells (ILCs) has revealed their significance in successful pregnancy. Sex hormones, such as estradiol and progesterone, show specific changes during pregnancy and modulate both adaptive and innate immune systems. ILC subset distribution in peripheral blood of pregnant women and its potential association with sex hormone levels have not been well revealed. Peripheral blood was obtained from healthy non-pregnant, early-pregnant, and late-pregnant women. Radioimmunoassay was performed to measure plasma estradiol and progesterone levels. The levels of type 1 ILCs (ILC1s), type 2 ILCs (ILC2s), type 3 ILCs (ILC3s), and total ILCs as well as estrogen and progesterone receptors of ILC2s in peripheral blood were analyzed using flow cytometry. The proportion of total ILCs and distribution of ILC subsets in peripheral blood changed dynamically during pregnancy. Compared to non-pregnant women, late-pregnant women displayed significantly higher proportion of circulating ILCs, among which ILC2s accounted for the majority in late-pregnant women while a smaller part in others, and ILC3s displayed the opposite. Plasma estradiol and progesterone levels elevated while pregnancy proceeded and the expression of their receptors in ILC2s increased consisted with the proportion of circulating ILC2s. Our work first observed the existence of progesterone receptors in human circulating ILC2s and revealed the distribution pattern of circulating ILC subsets and their interrelation with plasma sex hormone levels during pregnancy. Our results suggested that the estradiol and progesterone levels might partly influence the distribution of circulating ILC subsets and implied the interplay between circulating ILCs and pregnancy.

scholarly journals Increased expression of Fas on group 2 and 3 innate lymphoid cells is associated with an interferon signature in systemic lupus erythematosus and Sjögren’s syndrome

Rheumatology ◽  
2019 ◽  
Vol 58 (10) ◽  
pp. 1740-1745 ◽  
Author(s):  
Sofie L M Blokland ◽  
Lucas L van den Hoogen ◽  
Emmerik F A Leijten ◽  
Sarita A Y Hartgring ◽  
Ruth Fritsch ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Peripheral Blood ◽  
Innate Lymphoid Cells ◽  
Sjogren's Syndrome ◽  
Plasmacytoid Dendritic Cells ◽  
Lymphoid Cells ◽  
Type I ◽  
Healthy Controls ◽  
Type I Ifn ◽  
Group 2

Abstract Objective The role of innate lymphoid cells (ILCs) in the pathophysiology of rheumatic diseases is emerging. Evidence from animal studies implicate type I IFN, produced by plasmacytoid dendritic cells, to be involved in regulating the survival of group 2 and group 3 ILCs (ILC2s and ILC3s) via the upregulation of Fas (CD95) expression. For the first time, we explored the frequency and phenotype of circulating ILCs in SLE and primary Sjögren’s syndrome (pSS) in relationship to the IFN signature. Methods Frequencies and phenotypes of ILC subsets and plasmacytoid dendritic cells were assessed by flow cytometry in peripheral blood of patients with SLE (n = 20), pSS (n = 20) and healthy controls (n = 17). Patients were stratified by the presence or absence of an IFN signature as assessed by RT-qPCR on circulating mononuclear cells. Results ILC1 frequencies were increased in peripheral blood of patients with SLE as compared with healthy controls and correlate with disease activity in pSS patients. Overall, the frequencies of ILC2s or ILC3s did not differ between patients with SLE, pSS and healthy controls. However, patients with a high type I IFN signature expressed elevated levels of Fas on ILC2s and ILC3s, which coincided with decreased frequencies of these cells in blood. Conclusion The presence of a type I IFN signature is related to Fas expression and frequencies of circulating ILC2s and ILC3s in patients with SLE and pSS, potentially altering the homeostatic balance of ILCs.

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